• The Korean Journal of Mycology
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• v.25 no.3 s.82
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• pp.233-237
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• 1997

A PABA auxotroph of Pleurotus sajor-caju were transformed to prototrophy by using a plasmid containing pab 1 gene from Coprinus. The efficiencies of transformation of Pleurotus sajor-caju was five transformants per ${\mu}g$ of plasmid DNA. Southern blot analysis of DNA extracted from transformants demonstrated that plasmid DNA was integrated into the chromosomal DNA in multiple tandem copies. Progenies of heterokaryons between transformants of PABA and other auxotropic strains produced pab-progeny, which indicated that integration occurred at a site(s) other than the resident pab biosynthetic gene.

• Korean Journal of Microbiology
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• v.29 no.1
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• pp.16-24
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• 1991

The yeast gene THR1 encodes the homoserine kinase (EC 2.7.1.39: HKase) which catalyses the first step of the threonine specific arm at the end of the common pathway for methionine and threonine biosynthesis. A recombinant plasmid pMC3 (12.6 kilobase pairs, vector YCp50) has been cloned into E. coli HB101 from a yeast genomic library through its complementing activity of a thr1 mutation in a yeast recipient strain M39-1D. When subcloned into pMC32 (8.6kbp, vector YRp7) and pMC35 (8.3 kbp, vector YIp5), the HindIII fragment (2.7 kbp) of pMC3 insery was positive in the thrI complementing activity in both yeast and E. coli auxotrophic strains. The linearized pMC35 was introduced into the original recipient yeast strain and the mitotically stable chromosomal integrant was identified among the transformants. Through the tetrad analysis, the integration site of the pMC35 was localized to the region of THR1 structural gene at an expected genetic distance of approximately 11.1 cM from the ARG4 locus on the right arm of the yeast chromosome VIII. When episomically introduced into the auxotrophic cells and cultured in Thr omission liquid medium, the cloned gene overexpressed the HKase in the order of thirteen to fifteenfold, as compared with a wildtype. HKase levels are repressed by addition of threonine at the amount of 300 mg/l and 1, 190 mg/l for pMC32 and pMC3, respectively. Data from genetic analysis and HKase response thus support that the cloned HindIII yeast DNA fragment contains the yeast thr1 structural gene, along with necessary regulatory components for control of its proper expression.

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1503-1509
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• 2014

With the purpose of facilitating the process of stable strain generation, a shuttle vector for integration of genes via a double recombination event into two ectopic sites on the Sulfolobus acidocaldarius chromosome was constructed. The novel chromosomal integration and expression vector pINEX contains a pyrE gene from S. solfataricus P2 ($pyrE_{sso}$) as an auxotrophic selection marker, a multiple cloning site with histidine tag, the internal sequences of malE and malG for homologous recombination, and the entire region of pGEM-T vector, except for the multiple cloning region, for propagation in E. coli. For stable expression of the target gene, an ${\alpha}$-glucosidase-producing strain of S. acidocaldarius was generated employing this vector. The malA gene (saci_1160) encoding an ${\alpha}$-glucosidase from S. acidocaldarius fused with the glutamate dehydrogenase ($gdhA_{saci}$) promoter and leader sequence was ligated to pINEX to generate pINEX_malA. Using the "pop-in" and "pop-out" method, the malA gene was inserted into the genome of MR31 and correct insertion was verified by colony PCR and sequencing. This strain was grown in YT medium without uracil and purified by His-tag affinity chromatography. The ${\alpha}$-glucosidase activity was confirmed by the hydrolysis of $pNP{\alpha}G$. The pINEX vector should be applicable in delineating gene functions in this organism.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.09a
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• pp.695-699
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• 2009

3D and BIM technology are presumed to be used more and more in the construction industry. The scope of this paper arose from a general wondering of how the constructor in general can benefit from the use of 3D models. Presented herewith is the first application of BIM in Korea to a actual bridge construction site located in Cheongpoong in Chooncheongbook-Do. This paper tries to integrate design, cost and schedule under location-based conditions with industry-specific solution for 3D modeling. 292 activities constitute the cable-stayed bridge of 442m long with a main span of 327m long. Integration of 3D model, cost and schedule is shown by comparing the measurements of works at a specific time by use of 2 different construction sequencing scenarios and cost breakdown structures.

• Journal of Positioning, Navigation, and Timing
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• v.11 no.4
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• pp.327-332
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• 2022

The Korea Augmentation Satellite System (KASS) is the Satellite-Based Augmentation System (SBAS) under development in Korea. KASS navigation service support navigation Safety of Life (SoL) service. KASS signal provides corrections to Global Positioning System (GPS) data received from KASS Reference Stations (KRS) and is broadcast form Geostationary Earth Orbiting (GEO) satellites to KASS users and is used by GPS/SBAS user equipment to improve the accuracy, availability, continuity and integrity of the navigation solution. Seven KRS's collect the satellite data and send them to the KASS Processing Stations (KPS) for the generation of the corrections and the monitoring the integrity. For performing its computation the KPS needs to know accurate and reliable KRS antennas coordinates. These coordinates are provided as configuration parameters to the KPS. This means that the reference frame in which the KPS work is the one represented by the set of coordinates provided as input. Therefore, the activity to maintain the accuracy of the KRS antenna coordinates is necessary, knowing that coordinates can evolve due to earth plates movements or earthquakes. In this paper, we analyzed the geodetic survey results for KRS antenna coordinates from Site Acceptance Test (SAT) #1 in December 2020 to August 2022. In the future, it is expected that these activities and planning for KRS coordinates maintenance will be produced and provided to KASS system operators for KPS configuration updates during the KASS lifetime of 15 years. Through these maintenance activities, it is expected that monitoring and analysis of unpredictable events such as earthquakes and seism will be possible in the future.

• Journal of Fashion Business
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• v.13 no.6
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• pp.20-30
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• 2009

The purpose of this study is to develop the effective customized elderly fashion marketing process based on the web site, where older customer will be able to choose various fabrics and to try them out. This aims to establish new prototype of internet shopping mall for customized elderly fashion clothing. In this study, new method of product presentation on the online shopping mall is proposed to offer product information through 3D virtual reality. With the online shopping mall(SATC Mall) as a showcase, we presented virtual mapping system so that it enable the customers to select the fabrics and to see exactly how chosen fabric will look when applied to image of clothing. As an initial test of the application of simulation to measure 3D visualization of product, mapping software Vision Easy Map Pro Version 6.0(NedGraphics) Vision Easy Map Viewer Version 5.0(NedGraphics) were chosen and applied. By using this mapping system, the fabric change of the apparel product could be made on the internet shopping web site. However, this approach has been successful applied for presenting and customizing garment products. Future research will focus on the integration of mapping coordination into SATC Mall.

• Structural Engineering and Mechanics
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• v.30 no.3
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• pp.331-350
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• 2008

A framework for multi-platform analytical and multi-component hybrid (testing-analysis) simulations is described in this paper and illustrated with several application examples. The framework allows the integration of various analytical platforms and geographically distributed experimental facilities into a comprehensive pseudo-dynamic hybrid simulation. The object-oriented architecture of the framework enables easy inclusion of new analysis platforms or experimental models, and the addition of a multitude of auxiliary components, such as data acquisition and camera control. Four application examples are given, namely; (i) multi-platform analysis of a bridge with soil and structural models, (ii) multiplatform, multi-resolution analysis of a high-rise building, (iii) three-site small scale frame hybrid simulation, and (iv) three-site large scale bridge hybrid simulation. These simulations serve as illustrative examples of collaborative research among geographically distributed researchers employing different analysis platforms and testing equipment. The versatility of the framework, ease of including additional modules and the wide application potential demonstrated in the paper provide a rich research environment for structural and geotechnical engineering.

• Journal of Environmental Impact Assessment
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• v.4 no.1
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• pp.1-8
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• 1995

Environmental Impact Assessment(EIA) in Korea has developed as a tool for environmental conservation and decision making since Environmental Impact Statement(EIS) preparation in 1981. For effective implementation of it, we do system development and method enhancement continuously. The recently introduced GIS (Geographic Information System) can integrate geographic and attribute data, which will be applied to scoping, assessment, and alternative assessment, etc. in EIA. This study has three major components. First, it explains building of EIA factors of natural environment, living environment, and socio-economic environment of the study area defined in EIS preparation regulation. Second, the study presents applications of assessment method concerning suitability analysis of landfill site using GIS. Finally, it shows integration of GIS and odor impact assessment. Based on these analysis, the study makes some recommendations and conclusions.

• Korean Institute of Interior Design Journal
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• v.26 no.5
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• pp.16-24
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• 2017

This study examines Safdie's design philosophy: place, purpose, and process and design strategies found in museum buildings. Safdie, influenced by personal background as immigrant, education of McGil University in Canada, apprenticeship from Louis Kahn, is one of the most recognized architects who designed renowned public and cultural buildings after Habitat 67 in Montreal, Canada. In order to reveal design strategies his eight museum buildings has been selected and analyzed in terms of interpretation of context, program presented in floor plan and section design, and the process of integrity found in structure and material. The results of this paper can be summarized as follows: 1) Reflection of existing context and regeneration of the locality has been in a creative way to reinforce the sense of place. 2) The layout of major and gallery space is determined according to the Safdie's interpretation of the site context and this can be categorized into three types. 3) Integration of the innovative structural frame and material has been experimented by using tartan grid and toroid form in the major and gallery spaces. This study is intended to reevaluate the significance of Safdie's design approach to building uniqueness of the site, program and tectonic.

• Journal of Fashion Business
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• v.17 no.3
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• pp.1-14
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• 2013

This study is about the evaluation of the color arrangements of working clothes that are harmonized with working environment and assist in the protection of workers in industrial sites. This study attempted color arrangement design with the intention of giving order to the colors of working environment and working clothes so as to help improve the safety of work and the management aspects including human resource management, efficiency improvement, and the practice of business ethics. The study method consisted of literature research, empirical research, and evaluation research. The literature research performed theoretical examination about colors, industrial safety, and visibility. Three actual outdoor work sites in the machine industry were chosen and pictures were taken at 20-30 m from workers. The background colors of the work environment of each work site and the colors of the working clothes that were actually worn were analyzed through color values measured with a color-difference meter(CR-400). As a result, it was found that between value and chroma which affect visibility, color arrangement based on differences in chroma could be a greater influence than color arrangement based on differences in value. The results of this study could assist the integration of color schemes among working environment colors, people and working clothes by suggesting color arrangements with improved visibility that are applicable to the working clothes of domestic machine manufacturers that are playing a pivotal role in domestic industries and by presenting appropriate guidelines.