• Title/Summary/Keyword: Integration method

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Transformation of Chinese Cabbage Glutathione Reductase (GR) gene into Lettuce (Lactuca sativa L.) with Particle Bombardment (유전자총을 이용한 상추 내로의 배추 Glutathione Reductase (GR)유전자의 도입)

  • 정재동;이부자;이효신;김창길
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.6
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    • pp.475-478
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    • 2000
  • The cytosolic glutathione reductase(GR) gene of chinese cabbage was introduced into Lettuce (Lactuca sativa L.) with particle bombardment method. When cotyledon explants were treated with osmoticum-conditioning medium (0.6 M sorbitol/mannitol) 4 hours prior to and 16 hours after bombardment, it was identified by GUS assay that this condition was the most efficient one for introduction of foreign genes into cotyledon tissue of lettuce with particle bombardment. The stable integration of a GR gene was confirmed by the PCR analysis. 0.3, 0.6, 1.5 kbp PCR fragments of transgenes were obtained by three types of primers designed on the basis of GR sequence. To know whether the expression of the GR gene of pBKs-GR 1 can be stably maintained in the next generation, T$_2$selfing seeds obtained from the transformed mother plants were sowed on MS medium supplemented with 200 mg/L kanamycin sulfate. 70% of seedlings showed resistance to kanamycin.

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Integration of Ba0.5Sr0.5TiO3Epitaxial Thin Films on Si Substrates and their Dielectric Properties (Si기판 위에 Ba0.5Sr0.5TiO3 산화물 에피 박막의 집적화 및 박막의 유전 특성에 관한 연구)

  • Kim, Eun-Mi;Moon, Jong-Ha;Lee, Won-Jae;Kim, Jin-Hyeok
    • Journal of the Korean Ceramic Society
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    • v.43 no.6 s.289
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    • pp.362-368
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    • 2006
  • Epitaxial $Ba_{0.5}Sr_{0.5}TiO_3$ (BSTO) thin films have been grown on TiN buffered Si (001) substrates by Pulsed Laser Deposition (PLD) method and the effects of substrate temperature and oxygen partial pressure during the deposition on their dielectric properties and crystallinity were investigated. The crystal orientation, epitaxy nature, and microstructure of oxide thin films were investigated using X-Ray Diffraction (XRD) and Transmission Electron Microscopy (TEM). Thin films were prepared with laser fluence of $4.2\;J/cm^2\;and\;3\;J/cm^2$, repetition rate of 8 Hz and 10 Hz, substrate temperatures of $700^{\circ}C$ and ranging from $350^{\circ}C\;to\;700^{\circ}C$ for TiN and oxide respectively. BSTO thin-films were grown on TiN-buffered Si substrates at various oxygen partial pressure ranging from $1{\times}10^{-4}$ torr to $1{\times}10^{-5}$ torr. The TiN buffer layer and BSTO thin films were grown with cube-on-cube epitaxial orientation relationship of $[110](001)_{BSTO}{\parallel}[110](001)_{TiN}{\parallel}[110](001)_{Si}$. The crystallinity of BSTO thin films was improved with increasing substrate temperature. C-axis lattice parameters of BSTO thin films, calculated from XRD ${\theta}-2{\theta}$ scans, decreased from 0.408 m to 0.404 nm and the dielectric constants of BSTO epitaxial thin films increased from 440 to 938 with increasing processing oxygen partial pressure.

Design and Implementation of DMB Device Driver based on the Windows CE 5.0 (Windows CE 5.0 기반의 DMB 디바이스 드라이버 설계 및 구현)

  • Park, Kwang-Hee;Kim, Deok-Hwan;Kim, Young-Hoon;Chang, Joon-Hyuk
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.5
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    • pp.29-35
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    • 2007
  • Recently, as the demand of mobile multimedia devices increases and T-DMB is started in Korea, the need of research for integration of mobile devices such as cellular phone, navigation, and portable multimedia player becomes higher. In order to integrate mobile devices, it is necessary to support microprocessor with fast speed and various devices with multimedia service. In this paper, we construct Windows CE 5.0 platform whose BSP supports the embedded system board with ARM11 core and various devices and applications. We also implement the DMB device driver which supports busy waiting and interrupt driven I/O techniques, compare their performance, and then suggest the method to efficiently use the resources of embedded system.

Design of OTA Circuit for Current-mode FIR Filter (Current-mode FIR Filter 동작을 위한 OTA 회로 설계)

  • Yeo, Sung-Dae;Cho, Tae-Il;Shin, Young-Chul;Kim, Seong-Kweon
    • The Journal of the Korea institute of electronic communication sciences
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    • v.11 no.7
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    • pp.659-664
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    • 2016
  • In this paper, we suggest operational trans-conductance amplifier(OTA) for current-mode FIR filter that can be used in a digital circuit system requiring high operating frequency and low power consumption. The current-mode signal processing is one of the very innovative design method for a low power consumption system with high operating frequency because it shows a constant power regardless of frequency. From the simulation result using 0.35um CMOS process, when Vdd is 2V, it is confirmed that the proposed circuit showed the dynamic range of the about 1V, about 50% of supply voltage and output current swing of about 0~200uA. Also, the power consumption was evaluated with about 21uW and the active size for an integration was measured with $71um{\times}166um$.

An Analysis of Change on the Apartment Unit Plans and the Interior Spaces Related to Women (여성관련 공간을 중심으로 본 서울지역 아파트의 공간구조 변화)

  • Choi, Byung-Sook;Park, Jung-A
    • Journal of the Korean housing association
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    • v.20 no.4
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    • pp.39-47
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    • 2009
  • The purpose of this study is to find out the changes on the metropolitan condominium apartment unit plans based on the interior spaces related to women such An-bang (master bedroom), bathroom and attached spaces to the master bedroom, living room, diningroom, kitchen, utility room, and balcony. This study used the content analysis method and space syntax. Contents were limited to the floor plans with 3 rooms type and about 105 m2 in the Korean apartment encyclopedia. The unit plans of 2,278, built from October 1975 to January 2008, were analyzed and basically SPSS package 16.0 was employed. The results were as followed. 1) Living room was a core space and an increasing tendency. After 2001, the design trend of living room connected with kitchen and dining room, and dining room changed into one space combining with women's working space such kitchen. It meaned women's working activities became into family public activities as to depending open access. 2) Kitchen had a characteristics that was connected to utility room or back balcony, and the number of extra kitchen increased. Also, the size of DK decreased, but utility size little increased. It was showed the characteristic of this space was still for women. 3) An-bang had a little strong characteristics of married coulpe or women's personal space with bathroom or dress room/powder room. 4) In addition, the values of integration and control of living room and DK were highest. It meant these space were centered in unit plans. Next, the An-bang and dress room/powder room were high. Utility room or balcony's were next, and these were women's working space. Therefore, family's public space was most important, and married couple or women's personal and working space were later in planning.

Construct of Cooperative Security System for Secure MANET (안전한 MANET을 위한 협력적인 보안체계 구축)

  • Yang, Hwan Seok;Yoo, Seung Jae
    • Convergence Security Journal
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    • v.13 no.3
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    • pp.33-38
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    • 2013
  • Security between mobile nodes and efficient communication is one of the most important parts of the MANET. In particular, the wireless network is significantly higher for the attack threats because of collaborative structure for open communication media and communication. However, application of existing security mechanisms and intrusion detection system is not easy due to the characteristics of MANET. It is because collection and integration of adult data by the dynamic topology due to the mobility of nodes and many network sensors is difficult. In this study, we propose cooperative security system technique that can improve the reliability based on authentication assessing confidence about the whole nodes which joins to network and detect effectively this when intrusion occurs. Cluster head which manages the cluster performs CA role for the certificate issue and the gateway node performs role of intrusion detection system. Intrusion detection is performed by cooperating with neighboring nodes when attack is not detected in one intrusion detection node. The performance of the proposed method was confirmed through experiments comparing with the SRP technique.

A Study on the Dematerializing Tendency in Contemporary Space Design (공간디자인의 비(非)물질화 경향에 관한 연구)

  • 권영걸
    • Korean Institute of Interior Design Journal
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    • no.22
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    • pp.61-68
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    • 2000
  • This paper illustrates what the reality of architectural concepts, materials, notions and phenomena are as central questions of contemporay space design. These issues form the goal of modern space design which should attain to the reality of an era when non-real values prevail. Despite the trends of an everchanging and ephemeral dominating quality in architecture for the last ten years, architects still aim to contruct everlasting space on earth. The trends of dematerialization in today's space design can be substantiated in spatial-temporal dimensions as follows. First of all, ephemeral architecture with concepts of hypothetical temporality, everchanging architecture in fluidity, and the transparent architecture with the floating and overlapping image can be analyzed in the dimension of 'time'. In terms of 'space', void quality for the expression of emptiness, neutral space by the simplified and summarized forms, expanded space through ambiguous boundaries and spatial repetition can becharacterized and also be intended strategies for lightness, state of flux, ambiguity, paradox etc., lead modern space design along that path. As this point, we need to pay attention to the so-called 'hypersurface' concept proposed by Stephen Perella. Hypersurface is a sort of cladding sheathing existence independent from the primary structure. With it, the integration between form and image can be achieved. Sometimes hypersurface can be a strategical screen for image projection, a cognitive receptor for surroundings as well as a catalyst for information and communication systems. When the situation dematerializes more and more as the years go on, the concept of hypersurface can be an inclusive method between the phenomenological form in architecture and its self recipient image. Permissive atmospheres created between them in contemporary space design and new paradigms emerged with digital technology will further reinforce the human space's dynamism.

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Interprofessional Education Programs for Nursing Students: A Systematic Review (간호대학생을 위한 전문직 간 교육 프로그램에 대한 체계적 문헌고찰)

  • Park, Hayoung;Cho, Jinyoung;Chu, Sang Hui
    • The Journal of Korean Academic Society of Nursing Education
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    • v.24 no.3
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    • pp.235-249
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    • 2018
  • Purpose: The purpose of this study was to investigate how interprofessional education has been designed, implemented, and evaluated in undergraduate programs in nursing through a systematic review. Methods: The literature was searched using the PubMed, CINAHL, EMBASE, and Cochrane central databases to identify interventional studies including teaching-learning activities among nursing students and other disciplines in English between January 2000 and May 2017. Thirty studies were selected for the analysis. Results: Twenty-four studies out of 30 were designed as a pre-post, no control group, quasi-experimental study design. Interprofessional education learners were primarily engaged in medicine, physical therapy, dentistry, occupational therapy, pharmacy, and respiratory therapy. Patient care related activity was the most frequently selected topic and simulation was the most common teaching-learning method. Evaluation of learning outcomes was mainly based on the aspects of teams and collaboration, professional identity, roles and responsibilities, patient care, and communication skills. Nursing students in 26 out of the 30 reviewed studies were found to benefit from interprofessional education, with outcome effects primarily related to changes in learning outcomes. Conclusion: The development and integration of interprofessional education with collaborative practices may offer opportunities in nursing education for training professional nurses of the future.

Fatigue Behavior and Probabilistic Fatigue Analysis of Concrete Offshore Structures (콘크리트 해양구조물(海洋構造物)의 피로거동(疲勞擧動)과 확률론적(確率論的) 피로해석(疲勞解析))

  • Oh, Byung Hwan;Kim, Jee Sang
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.8 no.3
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    • pp.31-41
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    • 1988
  • Recently, the offshore structures are increasingly constructed to explore the natural resources. These offshore structures are to be designed to resist the repetitive wave forces. A probabilistic method for the fatigue analysis of offshore concrete structures is presented in this study. The present spectral fatigue analysis calculates wave forces first and then the transfer functions for unit waves from which stress spectra are determined. The calculated fatigue stresses may then be used to evaluate the fatigue damage of concrete structures. A simplified model for the estimation of fatigue damage of the structures, which employs only the probabilistic moments of the peak stress distribution without direct integration, is also proposed. The present study allows more realistic fatigue analysis of offshore concrete structures.

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Effectiveness of Isolation-System on Reduction of Seismic Response of Primary and Secondary Structures (주구조물 및 부구조물에 대한 감진장치의 지진응답 감소 효율성)

  • Kim, Young Sang;Lee, Dong Guen
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.12 no.4_1
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    • pp.9-21
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    • 1992
  • The effectiveness of the isolation system installed at the base of the primary structure and at the support of the substructure mounted on the primary structure is evaluated for reducing of structural responses under different earthquakes in this paper. The structural responses are analyzed to identify its behavior due to the input motion characteristics such as various peak acceleration and frequency content. Three analytical models are used to evaluate the effectiveness of the isolation system in this study as follows: fixed-base primary structure with support-fixed substructure, base-isolated primary structure with support-fixed substructure, and fixed-base primary structure with support-isolated substruciure. A computer code (KBISAP) is used for numerical integration of equation of motion considering the interaction between the primary structure and the secondary structure. The matrix condensation technique and constant average acceleration method are utilized in this program. And also, the effective stiffness of the base-isolator on reducing the structural response are evaluated for various earthquakes through the relationship of the acceleration - displacement.

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