• Journal of Microbiology and Biotechnology
• /
• v.16 no.1
• /
• pp.118-125
• /
• 2006

The region responsible for replication of the megaplasmid pAtC58 in the nopaline-type Agrobacterium tumefaciens strain C58 was determined. A derivative ofa Co1E1 vector, pBluscript SK-, incapable of autonomous replication in Agrobacterium spp, was cloned with a 7.6-kb Bg1II-HindIII fragment from a cosmid clone of pAtC58, which contains a region adjacent to the operon for the utilization of deoxyfructosyl glutamine (DFG). The resulting plasmid conferred resistance to carbenicillin on the A. tumefaciens strain UIA5 that is a plasmidfree derivative of C58. The plasmid was stably maintained in the strain even after consecutive cultures for generations. Analysis of nested deletions of the 7.6-kb fragment showed that a 4.3-kb BglII-XhoI region sufficiently confers replication of the derivative of the ColE1 vector on UIA5. The region comprises three ORFs, which have high homologies with repA, repB, and repC of plasm ids in virulent Agrobacterium spp. including pTiC58, pTiB6S3, pTi-SAKURA, and pRiA4b as well as those of symbiotic plasmids from Rhizobium spp. Phylogenie analysis showed that rep genes in pAtC58 are more closely related to those in pRiA4 than to pTi plasmids including pTiC58, suggesting that the two inborn plasmids, pTiC58 and pAtC58, harbored in C58 evolved from distinct origins.

• Composites Research
• /
• v.31 no.1
• /
• pp.37-41
• /
• 2018

The aim of the current work is to characterise a piecewisely-integrated composite bumper beam based on the IIHS bumper crash protocol. IIHS bumper crash FE analysis for an aluminium type bumper beam was carried out to get the information about the dominant loading types at several regions in the bumper beam during crash. In the meantime, robust stacking sequences against tension and compression have been searched for using FE analysis of a coupon type model. After determining most effective stacking sequences for tension and compression, three-point bending simulation was preliminarily carried out to investigate the combination performance of them. Finally, IIHS bumper crash FE analysis for the piecewisely-integrated composite bumper beam, which consisted of the combination of tension effective stacking sequence and compression efficacious stacking sequence, was conducted and the result was compared with other types of composite bumper beams. It was found that the newly suggested piecewisely-integrated composite bumper beam showed superior crashworthy behaviour to those of uni-modal stacking sequence composite bumper beams.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.38C no.3
• /
• pp.247-253
• /
• 2013

This paper analyzes ship area network(SAN) with integrated gateway. For the analysis, this paper analyzes reference network architecture, end-to-end connection model, traffic model, and requirement of SAN. Also, the type of network element and minimum performance of integrated gateway is derived by ship area network simulator. The performance evaluation results show that a integrated gateway operating as network switch with performance over than 30,000 packets/sec can provides end-to-end connection with satisfaction of requirement of each end-to-end connection.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.15 no.1
• /
• pp.15-23
• /
• 2015

This thesis proposes how data security has changed since the emergence of 'Big Data' in 2012 and the type of Integrated Security Management System that needs to be built against security threats, based on an analysis of Big Data. Much research has been conducted in Big Data. I need to think about what an Integrated Security Management System requires in order to safeguard against security threats such as APT. I would like to draw a comparison between the current Integrated Security Management System and one that is based on Big Data, including its limitations and improvements, so that I can suggest a much improved version of Integrated Security Management System.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.20 no.3
• /
• pp.262-272
• /
• 2015

An integrated two-in-one transformer applicable to PSUs for a 120 W LED TV is proposed. This transformer comprises a PFC inductor and an LLC transformer placed and integrated on an E-I-E type magnetic core. Performance is evaluated by observing the coupling coefficients of the proposed two-in-one transformer under various air gap topologies. Among the topologies studied, an integrated transformer with centered air gap shows stable operational characteristics with a minimized mutual coupling (interference). Furthermore, applicability of the proposed integrated transformer to PSUs for a 120 W LED TV is studied from the viewpoint of integrating different magnetic components into one core, resulting to low weight, low cost, and high power density.

• Current Photovoltaic Research
• /
• v.11 no.2
• /
• pp.54-57
• /
• 2023

Recently, requirements for improving the convenience of constructing BIPV (Building Integrated Photo Voltaic) modules had increased. To solve this problem, we fabricated shingled PV modules integrated with bent steel plates for building integrated photovoltaics. These PV modules could be constructed directly on the roof without the installation structure. We found optimal lamination conditions with supporting structures to fabricate a module on a bent steel plate. Moreover, we applied a shingled design to PV modules integrated with bent steel plates to achieve a high electrical output power. The shingled module with bent steel plates shows 142.80 W of solar-to-power conversion in 0.785 m² area.

• The Journal of the Korea Contents Association
• /
• v.20 no.7
• /
• pp.316-330
• /
• 2020

The study analyzes reviews of hardware products, customer service products, and products that take the form of a convergence of hardware and cloud services in ICT using text mining. We derive keywords of each review and find the differentiation of words that are used to derive topics. A cluster analysis is performed to categorize reviews into their respective clusters. Through this study, we observed which keywords are most often used for each product type and found topics that express the characteristics of products and services using topic modeling. We derived keywords such as "professional" and "technician" which are topics that suggest the excellence of the service provider in the review of service products. Further, we identified adjectives with positive connotations such as "favorite", "fine", "fun", "nice", "smart", "unlimited", and "useful" from Amazon Eco review, an integrated product and service. Using the cluster analysis, the entire review was clustered into three groups, and three product type reviews exclusively resulted in belonging to each different cluster. The study analyzed the differences whereby consumer needs are expressed differently in reviews depending on the type of product and suggested that it is necessary to differentiate product planning and marketing promotion according to the product type in practice.

• Journal of Life Science
• /
• v.34 no.2
• /
• pp.79-85
• /
• 2024

SlyA is known as a transcriptional regulator that regulates the expression of hemolysin (HlyE) in E. coli, a member of the Enterobacteriaceae family such as Salmonella. However, Salmonella has the slyA gene but lacks the hlyE gene. Then, because we were curious about the role of SlyA in Salmonella, we constructed and explored a mutant strain with a deletion of the slyA gene. S. Typhimurium CK295 (ΔslyA) was constructed using an allelic exchange approach. In a comparative analysis between the wild-type and the CK295 strain, no significant differences were observed in growth characteristics, motility, total protein analyses, and secreted protein analyses. However, the CK295 strain exhibited slightly reduced biofilm formation compared to the wild-type. Interestingly, as a result of comparing the survival ability in macrophages, the mutant strain showed a 60% decrease in survival ability compared to the wild-type. To evaluate toxicity in mice, mortality was measured after oral administration to 6-week-old BALB/c mice. As a result, the LD₅₀ value of the CK295 (ΔslyA) was more than 100 times higher than that of wild-type S. Typhimurium 𝜒3339 in BALB/c. In conclusion, SlyA is presumed to regulate the expression of genes encoding virulence factors involved in the in vivo survival of Salmonella.

• Journal of the Korean Chemical Society
• /
• v.59 no.2
• /
• pp.164-178
• /
• 2015

The purpose of this study was to design a teaching and learning method conductive to the development of creative thinking skills and investigate its effects. It has been developed integrated mind map with feature of visualizing the divergent thinking to the aspects of Science (S), Technology (T) & Engineering (E), Arts (A), Mathematics (M). Integrated mind map can be divided into four types of STEAM type, STEA type, STEM type, STE type depending on the category of key words in the first branch. And Integrated mind map can be divided into three levels of guided, intermediate, open depending on the teacher's guide degree. And also integrated mind map activities were carried out in the form of group, class share as well as individual. This study was implemented during a semester and students in experiment group experienced individual-integrated mind map activity 10 times, group-integrated mind map activity 10 times, class share-integrated mind map activity 3 times. The results indicated that the experimental group presented statistically meaningful improvement in creative thinking skills (p<.05). And there was a statistically meaningful improvement in fluency, flexibility, originality as a sub-category of creative thinking skills(p <.05). Also creative thinking skills are not affected by the level of cognitive, academic performance, gender (p<.05). In conclusion, it was found that 'integrated mind map activity' improved student's creative thinking skills. There was no interaction effect about creative thinking skills between the teaching strategy and cognitive level, achivement, gender of those students.

• Korean Journal of Applied Biomechanics
• /
• v.20 no.3
• /
• pp.277-284
• /
• 2010

The purpose of this study was to investigate the trunk rotation type by wheel and axle. In order to analysis, 3D-motion analysis and electromyography were conducted on kinematic variables, impulse, average-EMG and integrated-EMG. Twelve healthy (age: $21.8{\pm}2.2$ yrs, height: $175.4{\pm}5.0cm$, weight: $66.7{\pm}6.4kg$) participated in the experiment. The results were as follows; in hand's velocity and acceleration, wheel and axial rotating movement using kinematic chain(type 3) were much faster. In impulse, type 3 was much stronger. In average-EMG, right and left, latissimus dorsi muscles was much stronger. In integrated-EMG, left erector spinae, right/left latissimus dorsi, and left external oblique muscles was much stronger. These results considered that, in the trunk rotation utilizing the kinematic chains action, latissimus dorsi muscles highly contribute to the muscle utilization that makes the rotating movement maximally effective.