• KSBB Journal
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• v.21 no.5
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• pp.325-330
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• 2006

This study presents the characterization of an integrated portable microfluidic electrical detection system for fast and low volume immunoassay using polystyrene microbead, which are used as immobilization surfaces. In our chip, a filtration method using the microbead was adopted for sample immobilization and immunogold silver staining(IGSS) was used to increase the electrical signal. The chip is composed of an inexpensive and biocompatible Polydimethylsiloxane(PDMS) layer and Pyrex glass substrate. Platinum microelectrodes for electric signal detection were fabricated on the substrate and microchannel and pillar-type microfilters were formed in the PDMS layer. With a fabricated chip, we reacted antigen and antibody according to the procedures. Then, silver enhancer was injected to increase the size of nanogold particles tagged with the second antibody. As a result, microbeads were connected to each other and formed an electrical bridge between microelectrodes. Resistance measured through the electrodes showed a difference of two orders of magnitude between specific and nonspecific immuno-reactions. The detection limit was 10 ng/ml. The developed immunoassay chip reduced the total analysis time from 3 hours to 50 min. Fast and low-volume biochemical analysis has been successfully achieved with the developed microfilter and immuno-sensor chip, which is integrated to the microfluidic system.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.19 no.3
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• pp.179-184
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• 2019

In many countries, such as developing countries where electricity is scarce, small wind turbines in the form of Off Grid are an effective solution to solve power supply problems. In some countries, the expansion of power systems and the decline of electricity-intensive areas have led to the use of small wind power in urban road lighting, mobile communications base stations, aquaculture and seawater desalination. With this change, the size of the small wind power industry is expected to have greater potential than large-scale wind power. In the case of small wind power generators, the generator is controlled at a variable speed, and the voltage and current generated by the generator have many harmonic components. To solve this problem, the AC to DC converter to be studied in this paper is a three-phase step-up type converter with a single switch. The inductor current is controlled in discontinuous mode, and has a characteristic of having a unit power factor by eliminating the harmonic of the input current. The proposed converter is composed of LCL filter and three phase rectification boost converter at the input stage and a single phase full bridge for grid connection. It is a control system with energy storage system(ESS) that the system stabilization can be pursued against the electric power.

• KIEAE Journal
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• v.12 no.3
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• pp.41-46
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• 2012

The building envelope is important specially for saving energy consumption of residential buildings. but Apartment houses in Korea commonly have inside insulation system which have constantly arisen thermal bridges, the risk of heat loss, as a necessity. This study aims to evaluate integrated insulation performance according to the different shapes of external walls, adjacent to windows. The thermal performance analysis was carried out by Equivalent U-value and using the three-dimensional heat transfer computer simulation (TRISCO-RADCON), under nine different cases of comparing among three each of different bases(current standard model, 30percent energy saving model and 60percent energy saving model). The heating and the cooling load were also compared between two cases (standard U-value and Equivalent U-value) of three each of different bases, using the Building energy simulation which is based on DOE-2.1 analysis. As results, it turns out that if the Equivalent U-value is considered on the envelope analysis, the heat flow loss will be increasing more than the standard U-value, and if heat insulation property of the residential building reinforced rather than current, the rate of influences on the thermal bridges would be extremely expanded. In addition, it is shown that annual heating loads of the apartment house with applied Equivalent U-value substantially increased by more than 15 percent compared to those with the existing U-value, but annual cooling loads were negligibly affected.

• Spatial Information Research
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• v.22 no.5
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• pp.27-41
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• 2014

Recently, BIM(Building Information Modeling) has been applied to the infrastructure such as road and bridge, and information about the outside environment of buildings is needed for maintaining and managing the large urban facilities. In addition, the convergence between spatial information and Big-data has a large potentiality, in respect that considerable profits and developments in other application problems such as various simulations and urban plans, national land security, may be brought about on the basis of the interoperability of information between BIM and GIS. Therefore, this study attempted to suggest the development direction of a model integrating building for spatial information analysis and city on the subject by comparing and analyzing difference between information system and shape expression of IFC, CityGML and LandXML to efficiently link information between IFC as a standard model of BIM and CityGML as a standard model in the GIS sector and to prepare a basic fusion strategy and a method of utilization between BIM and GIS. The result of the study are as follow. Firstly, contents and structure of IFC, CityGML and LandXML are compared and analyzed. Secondly, the development direction of iBUM(Integrated Building and Urban Model) suggested, which is based on convergence technology for analysis of space information. Finally, a strategy and method of the BIM and GIS are proposed in the iBUM environment.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.301-310
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• 2010

Bacillus subtilis strains produce a broad spectrum of bioactive peptides. The lipopeptide surfactin belongs to one well-known class, which includes amphiphilic membrane-active biosurfactants and peptide antibiotics. Both the srfA promoter and the ComP-ComA signal transduction system are an important part of the factor that results in the production of surfactin. Bs-M49, obtained by means of low-energy ion implantation in wild-type Bs-916, produced significantly lower levels of surfactin, and had no obvious effects against R. solani. Occasionally, we found strain Bs-M49 decreased spore formation and the development of competence. Blast comparison of the sequences from Bs-916 and M49 indicate that there is no difference in the srfA operon promoter PsrfA, but there are differences in the coding sequence of the comA gene. These differences result in three missense mutations within the M49 ComA protein. RT-PCR analyses results showed that the expression levels of selected genes involved in competence and sporulation in both the wild-type Bs-916 and mutant M49 strains were significantly different. When we integrated the comA ORF into the chromosome of M49 at the amyE locus, M49 restored hemolytic activity and antifungal activity. Then, HPLC analyses results also showed the comA-complemented strain had a similar ability to produce surf actin with wild-type strain Bs-916. These data suggested that the mutation of three key amino acids in ComA greatly affected the biological activity of Bacillus subtilis. ComA protein 3D structure prediction and motif search prediction indicated that ComA has two obvious motifs common to response regulator proteins, which are the N-terminal response regulator receiver motif and the C-terminal helix-turn-helix motif. The three residues in the ComA N-terminal portion may be involved in phosphorylation activation mechanism. These structural prediction results implicate that three mutated residues in the ComA protein may play an important role in the formation of a salt-bridge to the phosphoryl group keeping active conformation to subsequent regulation of the expression of downstream genes.

• The Journal of the Convergence on Culture Technology
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• v.6 no.2
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• pp.535-541
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• 2020

A conventional elastic material replacement and performance evaluation are very complicated and time-consuming, and it is difficult to know when to replace the elastic material in advance. By comparing with the product limit and the functional limit, the necessity of elastic material replacement and the improvement of track support stiffness according to replacement can be immediately demonstrated based on experimental data. Using an evaluation system of track support stiffness, the performance evaluation data for elastic materials obtained through field tests using software for track support stiffness is integrated and managed on the administrator's computer. Therefore, the replacement plan is established and maintenance history is managed by identifying the replacement time and location of elastic materials. It is possible to evaluate the performance and condition of the elastic material at the various points during the working time of the track inspection and the track performance (track support stiffness) and durability of the elastic material (aging level, spring stiffness variation rate, etc.) at the operation condition. The elastic material could be replaced timely, and the deterioration of the elastic material can be continuously monitored.

• Journal of Wetlands Research
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• v.6 no.1
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• pp.167-178
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• 2004

The southern tidal flat of Kanghwa Island is one of the biggest flats on the west coast of Korea. Tide is typically a semidiurnal with maximum range of about 10m. The tidal flat receives large amount of sediments from Han River system. Surface sediments for sedimentary analyses were sampled at 83 stations in the study area in August 2003. The surface sediments consisted of five sedimentary facies. Generally, sandy mud sediments dominated in the southern tidal flat of Kanghwa Island, whereas sand sediments dominated in channel and subtidal zones of the western part of Kanghwa Island. The area of sandy mud sediment extended to eastward tidal flat compared to sedimentary facies in August 1997. Sedimentary facies analysis of three core sediments from the tidal flat to the south of the Kangwha Island revealed three sedimentary facies: trough-cross-bedded sand, laminated silt, and bioturbated silt. Distribution of the facies in the cores suggested that sedimentation rates has been generally high in the margin of main tidal channel, especially in the east of the Donggeum Island. Twelve-and-half-hour anchoring survey was carried out for measurements of hydrodynamic parameters at Yeomha channel near Choji Bridge(K1) and channel near Donggeum Island(K2) in June 2003. Residual flows indicated strong ebb-dominated tidal currents. Depth-integrated net suspended sediment loads for one tidal cycle were seaward movement with 309,217.9kg/m and 128,123.1kg/m at station K1 and K2, respectively. The higher value of net suspended sediment loads at station K1 suggested that lots of suspended sediments from Han River deposited in the eastern part of tidal flat.

• Journal of Korea Water Resources Association
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• v.55 no.12
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• pp.1041-1052
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• 2022

This study is to estimate the optimal ecological flow and analysis the spatial distribution of fish habitat for Andong dam downstream reach (4,565.7 km²) using PHABSIM (Physical Habiat Simulation System) and River2D. To establish habitat models, the cross-section informations and hydraulic input data were collected uisng the Nakdong river basic plan report. The establishment range of PHABSIM was set up about 410.0 m from Gudam streamflow gauging station (GD) and about 6.0 km including GD for River2D. To select representative fish species and construct HSI (Habitat Suitability Index), the fish survey was performed at Pungji bridge where showed well the physical characteristics of target stream located downstream of GD. As a result of the fish survey, Zacco platypus was showed highly relative abundance resulting in selecting as the representative fish species, and HSI was constructed using physical habitat characteristics of the Zacco platypus. The optimal range of HSI was 0.3~0.5 m/s at the velocity suitability index, 0.4~0.6 m at the depth suitability index, and the substrate was sand to fine gravel. As a result of estimating the optimal ecological flow by applying HSI to PHABSIM, the optimal ecological flow for target stream was 20.0 m³/sec. As a result of analysis two-dimensional spatial analysis of fish habitat using River2D, WUA (Weighted Usable Area) was estimated 107,392.0 m²/1000 m under the ecological flow condition and it showed the fish habitat was secured throughout the target stream compared with Q₃₅₅ condition.