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Microfluidic Immuno-Sensor Chip using Electrical Detection System  

Maeng, Joon-Ho (Department of Biochemistry, Graduate School, Hanyang University)
Lee, Byung-Chul (Division of Molecular and Life Science, Hanyang University)
Cho, Chul-Ho (Department of Precision Mechanical Engineering, Graduate School, Hanyang University)
Ko, Yong-Jun (Division of Molecular and Life Science, Hanyang University)
Ahn, Yoo-Min (Department of Mechanical Engineering, Hanyang University)
Cho, Nahm-Gyoo (Division of Molecular and Life Science, Hanyang University)
Lee, Seoung-Hwan (Division of Molecular and Life Science, Hanyang University)
Hwang, Seung-Yong (Division of Molecular and Life Science, Hanyang University)
Publication Information
KSBB Journal / v.21, no.5, 2006 , pp. 325-330 More about this Journal
Abstract
This study presents the characterization of an integrated portable microfluidic electrical detection system for fast and low volume immunoassay using polystyrene microbead, which are used as immobilization surfaces. In our chip, a filtration method using the microbead was adopted for sample immobilization and immunogold silver staining(IGSS) was used to increase the electrical signal. The chip is composed of an inexpensive and biocompatible Polydimethylsiloxane(PDMS) layer and Pyrex glass substrate. Platinum microelectrodes for electric signal detection were fabricated on the substrate and microchannel and pillar-type microfilters were formed in the PDMS layer. With a fabricated chip, we reacted antigen and antibody according to the procedures. Then, silver enhancer was injected to increase the size of nanogold particles tagged with the second antibody. As a result, microbeads were connected to each other and formed an electrical bridge between microelectrodes. Resistance measured through the electrodes showed a difference of two orders of magnitude between specific and nonspecific immuno-reactions. The detection limit was 10 ng/ml. The developed immunoassay chip reduced the total analysis time from 3 hours to 50 min. Fast and low-volume biochemical analysis has been successfully achieved with the developed microfilter and immuno-sensor chip, which is integrated to the microfluidic system.
Keywords
Immunoassay; IGSS; nanogold; PDMS; microfilter;
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1 Kissinger, P. T. (2005), Biosensors a perspective, Biosensors and Bioelectronics, 20, 2512-2516   DOI   ScienceOn
2 Khandurina, J. and A. Guttman (2002), Bioanalysis in microfluidic devices, Chromatography 943, 159-183   DOI   ScienceOn
3 Seydack, M. (2005), Nanoparticle labels in immunosensing using optical detection methods, Biosensors and bioelectronics, 20, 2454-2469   DOI   ScienceOn
4 Figeys, D. (2002), Adapting arrays and lab-on-a-chip technology for proteomics, Proteomics 2, 373-382   DOI   ScienceOn
5 Velev, O. D. and E. W. Kaler (1999), In Situ Assembly of Colloidal Particles into Miniaturized Biosensors, Langmuir 15, 11-17
6 Xue, M., J. Li, and Z. Lu, et al. (2002), Array-Based Electrical Detector of Integrated DNA Identification System for Genetic Chip Applications, ESSDERC, 483-486
7 Rosenzweig, Z. (2002), Development of an Aggregation-Based immunoassay for Anti-Protein A using Gold Nanoparticles, Analytical Chemistry 74, 1624-1628   DOI   ScienceOn
8 Hacker, G. W. and W. H. Muss (1996), Electron Microscopical Autometallo- graphy:Immunogold-Silver Staining (IGSS) and Heavy-Metal Histochemistry, Enzymology 10, 257-269
9 Luppa, P. B., L. J. Sokoll, and D. W. Chan (2001), Immunosensorsprinciples and applications to clinical chemistry, Clinica Chimica Acta 314, 1-26   DOI   ScienceOn
10 Rossier, J. S. and H. H. Girault (2001), Enzyme Linked immunosorbent assay on a microchip with electrochemical detection, Lab on a chip 1, 153-157   DOI   ScienceOn
11 Hagelsieb, L. M., P. E. Lobert, and R. Pampin, et al. (2004), Sensitive DNA electrical detection based on interdigitated Al/Al2O3 microelectrodes, Sensors and Actuators B 98, 269-274   DOI   ScienceOn
12 Hayes, M A., N. A. Polson, A. N. Phayre, et al. (2001), Flow-based Microimmunoassay, Analytical Chemistry 73, 5896-5902   DOI   ScienceOn