• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 제39회 하계학술대회
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• pp.1466-1467
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• 2008

We describe here a novel micro total analysis system for the purification and identification of the affinity-captured proteins. Also we demonstrated the mass analysis of the Carcinoembrionic antigen (CEA) and Alpha femtoprotein which were chosen as the target cancer marker. For MALDI-TOF analyses, the proteins should to be separated from a protein mixture and be concentrated when needed. This procedure usually takes a long time even before protease-digested samples are to be obtained from them. Here, we describe integrated and efficient micro chip for protein purification and digestion for MALDI-TOF analyses. At first, disease protein is purified by passing the micro chamber from a protein mixture or human whole serum and released from the micro affinity beads by thermal heating. Purified protein is then transfer to the hole for trypsin digestion. The final sample is analyzed by MALDI-TOF. All the processes could be finished successfully within one hour, which renders MALDI-TOF analyses of a target protein quite simple.

• Agricultural and Biosystems Engineering
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• 제1권2호
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• pp.106-110
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• 2000

An integrated on-line inspection system was constructed with seven cameras, half mirrors to split images. 720 nm and 970 nm band pass filters, illumination chamber having several tungsten-halogen lamps, one main computer, one color frame grabber, two 4-channel multiplexors, and flat plate conveyer, etc. A total of seven images, that is, one color image form the top of an apple and two B/W images from each side (top, right and left) could be captured and displayed on a computer monitor through the multiplexor. One of the two B/W images captured from each side is 720nm filtered image and the other is 970 nm. With this system an on-line grading software was developed to evaluate appearance quality. On-line test results with Fuji apples that were manually fed on the conveyer showed that grading accuracies of the color, defect and shape were 95.3%, 86% and 88.6%, respectively. Grading time was 0.35 second per apple on an average. Therefore, this on-line grading system could be used for inspection of the final products produced from an apple sorting system.

• Journal of Biosystems Engineering
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• 제17권4호
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• pp.344-353
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• 1992

This study was carried out to develop a fully-controlled plant growth system for studying the effects of environmental factors on plant growth. The plant growth system consists of the growth chamber, the measurement system for sensing and signal conditioning the environmental factors and the control system which includes control algorithm required for optimum operation and on-line monitoring. The results of the study on the performance of the controlled plant growth system are summarized as follows : 1. Under the light intensity of 18,000~20,000 lux, the temperature was controlled in the range of $22{\pm}0.4^{\circ}C$, the relative humidity of $70{\pm}5%$ respectively. 2. The $CO_2$ concentration was controlled in the range of $1,000{\pm}40ppm$(from 1st to 4th day) and $1,500{\pm}40ppm$(from 4th to 9th day). 3. The electric conductivity(EC) and pH of the nutrient solution were controlled in the range of $1.9{\pm}0.2mS/cm$ and $6.8{\pm}0.5pH$ respectively. 4. Under the above environmental conditions, the average fresh weight of leaf lettuce increased from 10g to 74g in 9 days with 24 hour lighting.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2009년도 추계학술대회 논문집
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• pp.594-599
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• 2009

Recent developments such as concern over global warming, depletion of fossil fuels and increase in energy demands by the increasing world population has eventually lead to mass production of electricity using renewable sources. Apart from wind and solar, ocean holds tremendous amount of untapped energy in forms such as geothermal vents, tides and waves. The current study looks at generating power using waves and the focus is on the primary energy conversion (first stage conversion) of incoming waves for different models. Observation of flow characteristics and the velocity in the augmentation channel as well as the front guide nozzle are presented in the paper. A numerical wave tank was used to simulate the waves and after obtaining the desired wave properties; the augmentation channel plus the front guide nozzle and rear chamber were integrated to the numerical wave tank. The waves in the numerical wave tank were generated by a piston type wave maker which was located at the wave tank inlet. The inlet which was modeled as a plate wall moved sinusoidally with the general function, x=asin$\omega$t The augmentation channel consisted of a front nozzle, rear nozzle and an internal fluid region representing the turbine housing. The analysis was performed using the commercial CFD code ANSYS-CFX.

• Bulletin of the Korean Chemical Society
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• 제26권6호
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• pp.939-942
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• 2005

For the detection of cyanobacterial toxin, an Enzyme-linked immunosorbent assay (ELISA) was integrated into a PDMS microchip. The conjugates of microcystin-LR (MCLR) and keyhole limpet hemocyanin (KLH) were adsorbed on the surface of polystyrene beads and these MCLR-KLH polystyrene beads were introduced into a microchamber. MCLR on the surface of polystyrene beads reacted with horseradish peroxides (HRP) conjugated anti-MCLR monoclonal antibody (mAb) which had a competitive reaction with MCLR in water sample. After the enzyme substrate 3,3,5,5-tetramethyl benzidine (TMB) was injected into the chamber and catalyzed by HRP, the color change was detected with a liquid-cord waveguide. This integration shortened the conventional ELISA analysis time from several hours to about 30 min with only 4.2 $\mu$L MCLR sample consuming which was useful for the environmental analysis. More over, troublesome operations required for ELISA could be replaced by simple operations. The microchip based detection system showed a good sensitivity of 0.05 $\mu$g/L and maintained good reliability through its quantitative range with low coefficients of variation (2.5-10.5%).

• 한국추진공학회지
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• 제7권2호
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• pp.44-53
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• 2003

KSR-III의 추진시스템 종합성능시험과 단인증시험을 위한 수직형 연소시험시설이 구축되었다. 이러한 시험은 발사체에 준하는 단품을 사용하는 시험으로, 상대적으로 낮은 수준의 안전율 하에서 시험이 진행되게 된다. 이에 연소시험 안전대책의 하나로 엔진부에 대한 비상정지 시스템이 검토되었으며, 정확하고 빠른 판단을 위하여 연소실 압력과 가속도신호를 사용하는 비상정지 시스템이 구축되었다. 이러한 측정변수를 통하여 점화지연 및 실패, 소화, 추진제 공급상태, 불안정 연소, 구조물 과도진동 등을 감시할 수 있었으며, 이상 상황인지 후 빠르게 시험을 중단할 수 있었다. 이처럼 빠른 판단과 후속조치로 시험의 안전을 확보할 수 있었으며, 목적한 개발시험을 안전하게 마칠 수 있었다.

• Nuclear Engineering and Technology
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• 제50권5호
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• pp.690-697
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• 2018

Criticality alarm systems (CASs) are mandatory in nuclear plants for prompt alarm in the event of any criticality incident. False criticality alarms are not desirable as they create a panic environment for radiation workers. The present article describes the design enhancement of the CAS at each stage and provides maximum availability, preventing false criticality alarms. The failure mode and effect analysis are carried out on each element of a CAS. Based on the analysis, additional hardware circuits are developed for early fault detection. Two different methods are developed, one method for channel loop functionality test and another method for dose alarm test using electronic transient pulse. The design enhancement made for the external systems that are integrated with a CAS includes the power supply, criticality evacuation hooter circuit, radiation data acquisition system along with selection of different soft alarm set points, and centralized electronic test facility. The CAS incorporating all improvements are assembled, installed, tested, and validated along with rigorous surveillance procedures in a nuclear plant for a period of 18,000 h.

• Nuclear Engineering and Technology
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• 제35권2호
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• pp.165-170
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• 2003

We developed an improved 3-PM liquid scintillation counting (3-PM LSC) method in which three detectors can be displaced to back and forth directions, and a data acquisition system being able to provide the values for all parameters required for the method. The detectors are entirely located in a 20-mm lead chamber of an inner diameter of 500 mm. A saw-toothed gear ties up all detectors so as to move them uniformly, up to 50 mm with unit of 1 mm. The data acquisition system was designed in an integrated circuit to perform the necessary works such as fast amplification, discrimination, coincidence and logic analysis. It generates values of nine parameters among twelve's generated in the 3-PH LSC method. The dead time of each counting channel is of extending type, valving from 10 to 100 $mutextrm{s}$. We measured the TDCR values with an unquenched liquid scintillation source 1"C by displacing the detectors with a step of 2.5 mm away from counting vial. Their values were derived on the range from 0.9 to 0.6. The extent is three times wider than those regions observed by applying the defocalization technique.ique.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 THE THIRD INTERNATIONAL CONFERENCE ON AGRICULTURAL MACHINERY ENGINEERING. V.III
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• pp.551-559
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• 2000

An integrated on-line inspection system was constructed with seven cameras, half mirrors to split images, 720 nm and 970 nm band pass filters, illumination chamber having several tungsten-halogen lamps, one main computer, one color frame grabber, two 4-channel multiplexors, and flat plate conveyer, etc., so that a total of seven images, that is, one color image from the top side of an apple and two B/W images from each side (top, right and left) could be captured and displayed on a computer monitor through the multiplexor. One of the two B/W images captured from each side is 720nm filter image and the other is 970nm. With this system an on-line grading software was developed to evaluate appearance quality. On-line test results to the Fuji apples that were manually fed on the conveyer showed that grading accuracies of the color, defective and shape were 95.3%, 86% and 91%, respectively. Grading time was 0.35 sec per apple on an average. Therefore, this on-line grading system could be used for inspection of the final products produced from an apple sorting system.

• 산업기술연구
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• 제43권1호
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• pp.15-23
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• 2023

Lithium-ion batteries are predominantly employed in electric vehicles and energy storage devices, offering the advantage of high energy density. However, they are susceptible to efficiency degradation when operated at high temperatures due to their sensitivity to the external environment. In this study, we conducted experiments using an indirect cooling method to prevent thermal runaway and explosions in lithium-ion batteries. The results were validated by comparing them with heat transfer simulations conducted through a commercial finite element analysis program. The experiments included single-cell exothermic tests and cooling experiments on a battery pack with 10 cells connected in series, utilizing 21700 lithium-ion batteries. To block external temperature influences, the experimental environment featured an extrusion method insulation in the environmental chamber. The cooling system, suitable for indirect cooling, was constructed with copper tubes and pins. The heat transfer analysis began by presenting a single-cell heating model using commercial software, which was then employed to analyze the heating and cooling of the battery pack.