Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Ultra-Sensitive Analysis of Microcystin LR Using Microchip Based Detection System

  • Pyo, Dong-Jin (Department of Chemistry, Kangwon National University) ;
  • Huang, Yan (Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology) ;
  • Kim, Young-Min (Central Research Institute of BodiTech Inc.) ;
  • Hahn, Jong-Hoon (Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology)
  • Published : 2005.06.20
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Abstract

For the detection of cyanobacterial toxin, an Enzyme-linked immunosorbent assay (ELISA) was integrated into a PDMS microchip. The conjugates of microcystin-LR (MCLR) and keyhole limpet hemocyanin (KLH) were adsorbed on the surface of polystyrene beads and these MCLR-KLH polystyrene beads were introduced into a microchamber. MCLR on the surface of polystyrene beads reacted with horseradish peroxides (HRP) conjugated anti-MCLR monoclonal antibody (mAb) which had a competitive reaction with MCLR in water sample. After the enzyme substrate 3,3,5,5-tetramethyl benzidine (TMB) was injected into the chamber and catalyzed by HRP, the color change was detected with a liquid-cord waveguide. This integration shortened the conventional ELISA analysis time from several hours to about 30 min with only 4.2 $\mu$L MCLR sample consuming which was useful for the environmental analysis. More over, troublesome operations required for ELISA could be replaced by simple operations. The microchip based detection system showed a good sensitivity of 0.05 $\mu$g/L and maintained good reliability through its quantitative range with low coefficients of variation (2.5-10.5%).

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References

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