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http://dx.doi.org/10.5012/bkcs.2005.26.6.939

Ultra-Sensitive Analysis of Microcystin LR Using Microchip Based Detection System  

Pyo, Dong-Jin (Department of Chemistry, Kangwon National University)
Huang, Yan (Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Kim, Young-Min (Central Research Institute of BodiTech Inc.)
Hahn, Jong-Hoon (Department of Chemistry, Division of Molecular and Life Sciences, Pohang University of Science and Technology)
Publication Information
Abstract
For the detection of cyanobacterial toxin, an Enzyme-linked immunosorbent assay (ELISA) was integrated into a PDMS microchip. The conjugates of microcystin-LR (MCLR) and keyhole limpet hemocyanin (KLH) were adsorbed on the surface of polystyrene beads and these MCLR-KLH polystyrene beads were introduced into a microchamber. MCLR on the surface of polystyrene beads reacted with horseradish peroxides (HRP) conjugated anti-MCLR monoclonal antibody (mAb) which had a competitive reaction with MCLR in water sample. After the enzyme substrate 3,3,5,5-tetramethyl benzidine (TMB) was injected into the chamber and catalyzed by HRP, the color change was detected with a liquid-cord waveguide. This integration shortened the conventional ELISA analysis time from several hours to about 30 min with only 4.2 $\mu$L MCLR sample consuming which was useful for the environmental analysis. More over, troublesome operations required for ELISA could be replaced by simple operations. The microchip based detection system showed a good sensitivity of 0.05 $\mu$g/L and maintained good reliability through its quantitative range with low coefficients of variation (2.5-10.5%).
Keywords
Microchip; Microcystin; Sensitive analysis;
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