• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2190-2198
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• 2017

Thermoactinomyces sp. strain YT06 was isolated from poultry compost and observed to degrade integral chicken feathers completely at $60^{\circ}C$, resulting in the formation of 3.24 mg/ml of free amino acids from 50 ml of culture containing 10 g/l chicken feathers. Strain YT06 could grow and secrete keratinase using feather as the only carbon and nitrogen sources without other supplement, but complementation of 10 g/l sucrose and 4 g/l $NaNO_3$ increased the production of the keratinolytic enzyme. The maximum protease activity obtained was 110 U/ml and for keratinase was 42 U/ml. The keratinase maintained active status over a broad pH (pH 8-11) and temperature ($60-75^{\circ}C$). It was inhibited by serine protease inhibitors and most metal ions; however, it could be stimulated by $Mn^{2+}$ and the surfactant Tween-20. A reductive agent (${\beta}$-mercaptoethanol) was observed to cleave the disulfide bond of keratin and improve the access of the enzyme to the keratinaceous substrate. Zymogram analysis showed that strain YT06 primarily secreted keratinase with a molecular mass of approximately 35 kDa. The active band was assessed by MALDI-TOF mass spectrometry and was observed to be completely identical to an alkaline serine protease from Thermoactinomyces sp. Gus2-1. Thermoactinomyces sp. strain YT06 shows great potential as a novel candidate in enzymatic processing of hard-to-degrade proteins into high-value products, such as keratinous wastes.

• 복식
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• 제65권6호
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• pp.1-14
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• 2015

This study is aims to study the background of how the shape of the wooden shoes developed and worn by Koreans and Japanese in the ancient times by sorting and examining the types and shapes. Relevant bibliography, which are mainly related to the excavated wooden shoes from 3 B.C.E to C.E. 8., were used as research materials. The formation of wooden shoes required easy access to raw materials -which is trees- for production. According to the analysis, both Korea and Japan made the flat wooden shoes using cuboid wood. Both countries bore a hole on a specific location to distinguish the right foot and left foot, however the style of the heel was different in the two countries. The slip-on(Undu-hyeong) wooden shoes were also common in both countries. The slip-on had no-heels and was made by digging-out a piece of the cuboid wood. Some slip-ons made by the Japanese had furrows on the bottom, and they were known to make different types of the slip-on wooden shoes depending on the purpose. Observation of the wearing methods show that commonality can be found between the wooden shoes of Korea and Japan, and this indicates that cultural exchanges between the Silla/Baekje and Japan took place from the 4th to the 6th century. Also, the flat wooden shoes in Japan developed rapidly, as the shoes became an integral part of its life and culture, which was closely tied to agriculture. Eventually, due to the difference in climate and life-style, the slip-ons became the primary type of wooden shoes in Korea, while the flats became the main type of wooden shoes in Japan. It is, however, clear that as the relationship between the two countries became closer, the cultural exchanges regarding the wooden shoes were considerable.

• 천문학회보
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• 제40권2호
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• pp.32.3-33
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• 2015

There exist scaling relations that link the mass of supermassive black holes with both the velocity dispersion and the mass of the central stellar cusp of their host galaxies. This implies that these two components grow in tandem. Feedback from actively accreting supermassive black holes (AGN), in the form of multi-phase gas outflows, has been argued to be the agent of this co-evolution. Here we employ the powerful GMOS integral field spectroscopy unit on the 8.2m Gemini-North telescope to investigate ionized gas outflows of luminous Type 2 AGN in the local Universe (z<0.1). Our sample of 6 galaxies is drawn from the Sloan Digital Sky Survey (SDSS) and was selected based on their [OIII] dust-corrected luminosity (>1042 erg/s) and signatures of outflows in the [OIII] line profile of their spatially integrated SDSS spectra. These are arguably the best candidates to explore AGN feedback in action since they are < 1% of a large local type 2 AGN SDSS sample selected based on their [OIII] kinematics. We combine a careful spectral decomposition of the [OIII] and $H{\alpha}$ line profiles with spatial information on ~0.5kpc scales to understand the outflow kinematics and energetics in these objects. We find clear evidence for strong outflows in [OIII] and occasionally $H{\alpha}$ that are clearly driven by the ionizing radiation of the AGN. We kinematically and spatially decompose outflowing and rotating ionized gas components. We find [OIII] to be a better tracer of AGN outflows, while $H{\alpha}$ appears to be strongly affected by both stellar rotation and outflows induced by ongoing star formation. The observed kinematics and spatial distribution of the ionized gas imply a large opening angle for the outflow. Finally, we find the projected outflow velocity to decrease as a function of distance, while its dispersion shows a more complex structure with a potentially initially increasing trend (out to 0.5-1kpc distances).

• Nuclear Engineering and Technology
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• 제52권8호
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• pp.1706-1713
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• 2020

Functionally gradient material (FGM) is promisingly effective in mitigating the thermal stress between plasma facing materials (PFM) and structural materials. However, the corresponding research with respect to W/V FGM has not been reported yet. In this work, we firstly report the successful fabrication of W/V FGM by a combined technology of mechanical alloying (MA) and spark plasma sintering (SPS). The microhardness and microstructure of the consolidated sample were both investigated. W/V stacks show significantly enhanced microhardness (>100%) compared with pure W plate, which is beneficial to the integral strength of the hybrid structure. Furthermore, we clarify that the different ductility of W and V should be carefully considered, otherwise W/V powder might aggregate and lead to the formation of compositional segregation, and simultaneously unmask the impact of V proportion on the distribution of second phase in W-V binary alloy system. This work provides an innovative approach for obtaining W-V connections with much better performance.

• 천문학회보
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• 제39권2호
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• pp.95-95
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• 2014

The mass measurement of neutron stars or black holes is of fundamental importance in our understanding of the evolution of massive stars and core-collapse supernova explosions as well as some exotic physics of the extreme conditions. Despite the importance, however, it's very difficult to measure mass of these objects directly. One way to do this, if they are in binary systems, to measure their binary motions (i.e., Doppler shifts) which can give us direct information on their mass. Recently many new highly-obscured massive X-ray binaries have been discovered by new hard X-ray satellites such as INTEGRAL and NuSTAR. The new highly-obscured massive X-ray binaries are faint in the optical, but bright in the infrared with many emission lines. Based on the near-infrared spectroscopy, one can first understand the nature of stellar companions to the compact objects, determining its spectral types and luminosity classes as well as mass losses and conditions of (potential) circumstellar material. Next, spectroscopic monitoring of these objects can be used to estimate the mass of compact objects via measuring the Doppler shifts of the lines. For the former, broad-band spectroscopy is essential; for the latter, high-resolution spectroscopy is critical. Therefore, IGRINS appears to be an ideal instrument to study them. An IGRINS survey of these new highly-obscured massive X-ray binaries can give us a rare opportunity to carry out population analyses for understanding the evolution of massive binary systems and formation of compact objects and their mass ranges. In this talk, we will present a sample near-infrared high resolution spectra of HMXB, IGR J19140+0951 and discuss about its spectral feature. These spectra are obtained on 13th July, 2014 from IGRINS commissioning run at McDonald 2.7m telescope. And at final, we will introduce the upgrade plan of IGRINS Operation Software (IGOS), to gather the input from IGRINS observer.

• Human Ecology Research
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• 제52권5호
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• pp.457-471
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• 2014

Family instability has a negative effect on not only an individual household but also the society. The government continues to operate family support centers as a countermeasure to family instability issues. A healthy and multicultural family support center is considered an effective support system that extends from a family unit to the local community. In this study, the satisfaction and demand levels of each center, located in Seoul and Incheon, were examined with respect to items such as operation contents, physical space, and organizational structure. The results showed that various efforts for creating a family-friendly community were implemented in healthy family support centers and multicultural family support centers. It was found that the center organizers and operators focused more on software and programs than on the physical environment. There was mostly a shortage of physical and environmental space. This space shortage limited the planned and ongoing activities in terms of the residents' self-directed participation and space formation for a family-friendly educational culture. Therefore, it was necessary to set up the space-related criteria for each center in order to solve this environmental issue. We identified the baseline data of the criteria for a center's physical and spatial design and size. In particular, it was suggested that a more strategic physical and spatial design is needed to achieve an integral and effective operation for the connection of the family with the local society.

• 천문학회보
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• 제42권1호
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• pp.28.1-28.1
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• 2017

To investigate AGN outflows as a tracer of AGN feedback on the host galaxies, we perform integral-field spectroscopy of 20 type 2 AGNs at z<0.1 using the Magellan/IMACS and the VLT/VIMOS. The observed objects are luminous AGNs with the [O III] luminosity >$10^{41.5}erg/s$, and exhibit strong outflow signatures in the [O III] kinematics. We obtain the maps of the narrow and broad components of [O III] and $H{\alpha}$ lines by decomposing the emission-line profile. The broad components in both [O III] and $H{\alpha}$ represent the non-gravitational kinematics, (i.e., gas outflows), while the narrow components represent the gravitational kinematics (i.e., rotational disks), especially in $H{\alpha}$. By using the spatially integrated spectra within the flux-weighted size of the narrow-line region, we estimate the outflow energetics. The ionized gas mass is $(1.0-38.5){\times}10^5M_{\odot}$, and the mean mass outflow rate is $4.6{\pm}4.3M_{\odot}/yr$, which is a factor of ~260 higher than the mean mass accretion rate $0.02{\pm}0.01M_{\odot}/yr$. The mean energy injection rate is $0.8{\pm}0.6%$ of the AGN bolometric luminosity Lbol, while the mean momentum flux is $(5.4{\pm}3.6){\times}L_{bol}/c$, except for two most kinematically energetic AGNs. The estimated energetics are consistent with the expectations for energy-conserving outflows from AGNs, yet we do not find any supporting evidence of instantaneous star-formation quenching due to the outflows.

• 한국세라믹학회지
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• 제45권7호
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• pp.411-417
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• 2008

Aerosol Deposition Method (ADM) is a novel technique to grow ceramic thick films with high density and nano-crystal structure at room temperature. $^{1,2)}$ For these unique advantages of ADM, it would be applied to the fabrication process of 3-D integration ceramic modules effectively. However, it is critical to control the properties of starting powders, because a film formation through ADM is achieved by impaction and consolidation of starting powders on the substrates. We fabricated alumina thick films by ADM for the application to integral substrates for RF modules. When the as-received alumina powders were used as a starting material without any treatments, it was observed that the dielectric properties of as-deposited alumina films, such as relative permittivity and loss tangent, showed high dependency on the frequency. In this study, some techniques of powder pre-treatments to improve the dielectric properties of alumina thick films will be shown and the effects of starting powders on the properties of AD films will be discussed.

• 복식
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• 제59권7호
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• pp.127-139
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• 2009

The purpose of this study is to research the change of Tibet's commercial district following Tibet's globalization(traditional clothing to modern clothing). Tibet's traditional culture is fastly changing for two major reasons: Chinese government's persistent assimilation policy on minority ethnic groups and globalization, a powerful trend worldwide. Therefore this investigation was conducted on the most preeminent feature of life, clothing culture, at capital city Lhasa where modernization is most prominent and fast in Tibet. For this, the first field investigation was conducted between February 5th and 15th, 2007. and the second investgation was between January 16th and 25th, 2008. As a result, the study on clothing globalization in Lhasa, Tibet, reaches the conclusion as follows: Based on such developments, commerce of Lhasa has been modernized, bringing about great change in composition and formation of its commercial district. Stores have been modernized and their service quality has improved. While the number of traditional clothes shops has decreased, various types of modern clothes shops have emerged. Modern clothes stores mostly consist of quality men's wear shops, casual clothing shops targeting those in their 20s, and sportswear shops reflecting global trend. This composition indicates that it is men and younger generation who first adopt new culture emerged through globalization. Tibet's modernization and social development have become an integral part of globalization and public sentiment. Therefore, its modernization will be driven by power and capability of the public, rather than by policy control of the central government.

• Journal of Periodontal and Implant Science
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• 제38권sup2호
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• pp.273-298
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• 2008

Purpose: The aim of this review is to introduce a novel bone-graft material for hard-tissue regeneration based on the calcium phosphate glass(CPG). Materials and Methods: CPG was synthesized by melting and subsequent quenching process in the system of CaO-$CaF_2-P_2O_5$-MgO-ZnO having a much lower Ca/P ratio than that of conventional calcium phosphates such as HA or TCP. The biodegradability and bioactivity were performed. Effects on the proliferation, calcification and mineralization of osteoblast-like cells were examined in vitro. Influence in new bone and cementum formations was investigated in vivo using calvarial defects of Sprague-Dawley rats as well as 1-wall intrabony defect of beagle dogs. The application to the tissue-engineered macroporous scaffold and in vitro and in vivo tests was explored. Results: The extent of dissolution decreased with increasing Ca/P ratio. Exposure to either simulated body fluid or fetal bovine serum caused precipitation on the surface. The calcification and mineralization of osteoblast-like cells were enhanced by CPG. CPG promoted new bone and cementum formation in the calvarial defect of Sprague-Dawley rats after 8 weeks. The macroporous scaffolds can be fabricated with $500{\sim}800{\mu}m$ of pore size and a three-dimensionally interconnected open pore system. The stem cells were seeded continuously proliferated in CPG scaffold. Extracellular matrix and the osteocalcin were observed at the $2^{nd}$ days and $4^{th}$ week. A significant difference in new bone and cementum formations was observed in vivo (p<0.05). Conclusion: The novel calcium phosphate glass may play an integral role as potential biomaterial for regeneration of new bone and cementum.