• 제목/요약/키워드: Instrumental analyses

검색결과 145건 처리시간 0.021초

호염세균 Haloarcular sp. EH-1으로부터 추출한 카로테노이드 색소의 특성 (Characteristic of Carotenoid Component from Halophilic Bacteria, Haloarcular sp. EH-1)

  • 정영기;최병대;강석중;정성훈;이용규;김해윤;정명주
    • KSBB Journal
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    • 제15권6호
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    • pp.673-676
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    • 2000
  • 호염성 세균이 갖는 특성 즉, 다른 균에 의한 오염 감소, 세포벽 파괴의 수월성, 용매를 이용하지 않는 색소추출 등을 응용하기 위한 기초 자료를 얻기 위하여 호염성 세균인 Haloarcular sp. EH-1 으로부터 추출한 천연색소를 bioreactor 에서 배양한 후 색소의 함량을 조사한 결과 3 L 용량에서는 8 83.1 mg%, 5 L 용량에서는 82.7 mg%의 카로테노이드를 함 유하는 것으로 나타났다 이들의 주된 성분은 $\beta$-c따otene (8.1 %), $\beta$-hydroxyechinenone (42.0%) 및 astaxanthin(25.0% )으로 동정 되었으며, 이들 성분을 동정하기 위하여 표품과의 co- TLC, co-HPLC, 가시부 홉수스펙트럼, 화학적 변화 등을 조사하였다.

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소셜네트워크 활용이 사회자본 형성에 미치는 영향 : 트위터, 페이스북, 카카오스토리를 중심으로 (The Effect of Social Network Usage on the Formation of Social Capital : Based on Twitter, Facebook, KakaoStory)

  • 홍삼열;오재철
    • 한국전자통신학회논문지
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    • 제7권6호
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    • pp.1471-1477
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    • 2012
  • 소셜네트워크는 다양한 콘텐츠와의 결합을 통하여 네트워크를 통해 형성된 지인 관계를 기반으로 공유와 배포를 원활하게 하는 주요 플랫폼으로 활용되는 등 몇 가지의 공통적인 기능을 가지고 있다. 이에 트위터, 페이스북, 카카오스토리를 모두 사용하고 있는 사용자들을 대상으로 설문조사를 진행하였으며, 설문에 응답한 자료를 통계 내어 각각의 서비스가 영향을 주는 요인들은 어떤 것인지 완성적 사회자본과 도구적 사회자본으로 나누어 분석한 결과를 제시하였다. 이 연구는 사용자들의 목적에 따라 소셜네트워크를 선택하는 기준이 되고, 새로운 소셜네트워크를 개발하거나 기존의 서비스를 발전시키는데 기여할 수 있을 것이다.

대학교육에서의 CHANGE 플립러닝(Flipped Learning) 수업모형 개발 -교육방법및교육공학교과를 중심으로- (The Development of CHANGE Flipped Learning Instructional Model in Higher Education - base on the 'educational method and technology')

  • 정주영
    • 수산해양교육연구
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    • 제28권6호
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    • pp.1834-1847
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    • 2016
  • Main objectives of the this study are: to develop a model of "Flipped Leaning" that is designed to enhance self-directed learning, learning motivation and self-control, and to verify its effectiveness-in higher education. The verification process initially concentrated on the feasibility study of the model with a thorough literature review and case analyses; then, its general and practical applicability were tested with a field study. As a result, first, the CHANGE Class Model, specifically designed for effective and efficient "Flipped Learning", was developed. It is thus named for the stages that the learning process takes place in the model-i.e., (1) Check ${\rightarrow}$ (2) Ask ${\rightarrow}$ (3) Notice ${\rightarrow}$ (4) Group presentation ${\rightarrow}$ (5) Evaluation, and it emphasizes the dynamic, questions centered (i.e. back and forth between the students and the instructor as well as between the students) learning process. Second, the Model was instrumental in enhancing self-directed learning, learning motivation and self-control; thus, as a result, it significantly improved the effectiveness, the level of concentration and the attractiveness of the learning process. The value of this study lies in pointing to a clear plan to allow a student in higher learning to set-up a self-directed learning plan, to be able to control it while being continuously motivated to complete it.

Rapid Determination of Volatile Organic Compounds in Human Whole Blood Using Static Headspace Sampling with Gas Chromatography and Mass Spectrometry

  • Lee, Ji-Young;Kim, Seungki;Lee, Jong-Tae;Choi, Jong-Ho;Lee, Jeongae;Pyo, Heesoo
    • Bulletin of the Korean Chemical Society
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    • 제33권12호
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    • pp.3963-3970
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    • 2012
  • Headspace (HS) and headspace solid-phase microextraction (HS-SPME) were studied for extracting volatile organic compounds (VOCs) from whole blood, with chemical and instrumental variables being optimized for maximum sensitivity: incubation at $60^{\circ}C$, equilibration for 30 min, pH 11, and 2 mL injection volume. Both techniques provided accurate analyses, with detection limits of 0.05-0.1 ng $mL^{-1}$ and 0.05-0.5 ng $mL^{-1}$. HS showed better sensitivity, reproducibility, and analysis times than HS-SPME. Overall levels of chloroform in whole blood were found to be 0.05-5.84 ng $mL^{-1}$; detected levels of benzene were 0.05-2.20 ng $mL^{-1}$.

Streptomyces sp. DMCJ-49 균주가 생산하는 Alpha-Amylase 저해제 MB4-03의 분리와 구조분석 (Isolation and Structural Analysis of MB4-03, an $\alpha$-Amylase Inhibitor Produced by Streptomyces sp. DMCJ-49)

  • 염대현;최응철;김병각;김진웅
    • 한국미생물·생명공학회지
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    • 제18권4호
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    • pp.338-343
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    • 1990
  • Streptomyces sp. DMCJ-49 균주의 배양액으로부터 분리된 $\alpha$-amylase 저해물질 MB4-03은 구조상 질소원자와 methyl기를 포함하는 일종의 직선형 Alpha(1-4) 결합으로 연결된 glucose의 변형된 oligomer 형태로서 구성 단당류는 12 정도로 추정된다.

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Identification of New Urinary Metabolites of Byakangelicin, a Component of Angelicae dahuricae Radix, in Rats

  • Kwon, Oh-Seung;Song, Yun-Seon;Shin, Kuk-Hyun;Ryu, Jae-Chun
    • Archives of Pharmacal Research
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    • 제26권8호
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    • pp.606-611
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    • 2003
  • Byakangelicin, 9-(2,3-dihydroxy-2-methylbutoxy)-4-methoxy-7H- furo[3,2-g][l]benzopyran-7-one (BKG), a component of Angelicae dahuricae Radix, is considered to be an inhibitor of aldose reductase for the treatment of diabetic cataract. An analytical method for the isolation of BKG developed by high-performance liquid chromatography has been reported. No literature on the metabolism of BKG, however, has been found. With the purpose of identifying new metabolites of BKG, BKG (100 mg/kg) was orally administered to Sprague-Dawley rats via a gavage. Using a metabolic cage, urine was collected for 24 h, and the urine samples were extracted by liquid-liquid extraction. For structural identification of new urinary metabolites of BKG, various instrumental analyses were conducted by gas-chromatography/mass spectrometry, high-performance liquid chromatography/diode array detector, liquid chromatography/mass spectroscopy with thermospray interface and $^1H$ nuclear magnetic resonance spectroscopy. Two metabolites produced from the Ο-demethylation or Ο-dealkylation of BKG were newly identified, and another new but unknown metabolite was assumed to be the hydroxylated form of BKG. These results indicate that the major metabolic products of BKG are formed by Ο-demethylation or Ο-dealkylation of BKG side chains.

A case study for determination of seismic risk priorities in Van (Eastern Turkey)

  • Buyuksarac, Aydin;Isik, Ercan;Harirchian, Ehsan
    • Earthquakes and Structures
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    • 제20권4호
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    • pp.445-455
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    • 2021
  • Lake Van Basin, located in Eastern Turkey, is worth examining in terms of seismicity due to large-scale losses of property and life during the historical and instrumental period. The most important and largest province in this basin is Van. Recent indicators of the high seismicity risk in the province are damage occurring after devastating earthquakes in 2011 (Mw=7.2 and Mw=5.6) and lastly in 2020 Khoy (Mw=5.9). The seismic hazard analysis for Van and its districts in Eastern Turkey was performed in probabilistic manner. Analyses were made for thirteen different districts in Van. In this study, information is given about the tectonic setting and seismicity of Van. The probabilistic seismic hazard curves were obtained for a probability of exceedance of 2%, 10% and 50% in 50-year periods. The PGA values in the Van province vary from 0.24 g - 0.43 g for earthquakes with repetition period of 475 years. Risk priorities were determined for all districts. The highest risk was calculated for Çaldıran and the lowest risk was found for Gürpınar. Risk priorities for buildings in all districts were also determined via rapid seismic assessment for reinforced-concrete and masonry buildings in this study.

Assessment of seismic risk of a typical RC building for the 2016 Gyeongju and potential earthquakes

  • Jee, Hyun Woo;Han, Sang Whan
    • Earthquakes and Structures
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    • 제20권3호
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    • pp.337-351
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    • 2021
  • On September 12, 2016, the Gyeongju earthquake occurred in the south-eastern region of the Korean peninsula. The event was ranked as the largest magnitude earthquake (=5.8) since instrumental recording was started by the Korean Metrological Administration (KMA) in 1978. The objective of this study is to provide information obtained from the 2016 Gyeongju earthquake and to propose a procedure estimating seismic risk of a typical old RC building for past and potential earthquakes. Ground motions are simulated using the point source model at 4941 grid locations in the Korean peninsula that resulted from the Gyeongju earthquake and from potential future earthquakes with the same hypocenter considering different soil conditions. Nonlinear response history analyses are conducted for each grid location using a three-story gravity-designed reinforced concrete (RC) frame that most closely represents conventional old school and public buildings. Then, contour maps are constructed to present the seismic risk associated with this building for the Gyeongju earthquake and potential future scenario earthquakes. These contour maps can be useful in the development of a mitigation plan for potential earthquake damage to school and public buildings at all grid locations on the Korean peninsula.

Ti-Al-Si-Cu-N 후막의 Cu 조성에 따른 기계적 특성과 미세구조 변화에 관한 연구 (Influence of Cu Composition on the Mechanical Properties and Microstructure of Ti-Al-Si-Cu-N thick films)

  • 이연학;허성보;박인욱;김대일
    • 한국표면공학회지
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    • 제56권5호
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    • pp.335-340
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    • 2023
  • Quinary component of 3㎛ thick Ti-Al-Si-Cu-N films were deposited onto WC-Co and Si wafer substrates by using an arc ion plating(AIP) system. In this study, the influence of copper(Cu) contents on the mechanical properties and microstructure of the films were investigated. The hardness of the films with 3.1 at.% Cu addition exhibited the hardness value of above 42 GPa due to the microstructural change as well as the solid-solution hardening. The instrumental analyses revealed that the deposited film with Cu content of 3.1 at.% was a nano-composites with nano-sized crystallites (5-7 nm in dia.) and a thin layer of amorphous Si3N4 phase.

기혼여성의 비정규 노동과 일/가족 갈등 -학습지 교사를 중심으로 (Married Women's contingent Work and Work/Family Conflicts - Concentrating on Study Paper Instructors)

  • 김경화
    • 가족자원경영과 정책
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    • 제10권2호
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    • pp.87-105
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    • 2006
  • The goal of this study is to analyze the complicated and diverse nature of the relationship between work and family life for the study paper instructors of whom are married women. The survey data for these analyses were from an in depth interview which was conducted with the 21 contingent female workers as study samples. They are the married women, who started to work or returned to work after an absence to raise a family. The results showed that in reality the contingent female worker faced worse working conditions than the full time female labour force, even if women were determined to be a study paper instructor to meet work and family demand. It was the contingent women worker with a short time work experience who were in the worst position tlo adjust their working arrangements to suit their family needs and were confronted with the greater inter-role friction as a vulnerable group. The study revealed that instrumental and emotional support of husband has a positive effect on maintaining job role and lessening role conflicts and stress of the women. The work/family relationship indicated its double side, conflict and support. The women simply could not afford to depend on the psychological support form family in the midst of the inferior employment conditions and the absence of social support. This strategy, however, was based on the family ideology and the patriarchal gender division of labor. It caused the overload, stress and poor health of women involving some risk to give up the work.

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