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Studies on Cryopreservation of D-shaped and Umbo Larvae of Arkshel1(Scapharca broughtonii)

  • K.H. Kang;K. H. Kho;Z.T. Chen;Kim, Y.H.;Kim, J.M.
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.72-72
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    • 2003
  • The present study examined the possibility of cryopreservation of the D-shaped and umbo larvae of arkshell (Scapharca broughtonii), in terms of the survival rates after freezing and thawing. D-shaped and umbo larvae of arkshells were obtained from a shellfish farming on Yosu city. The average shell lengths were $93.3 \pm 10.1 \mu$m and $201.7 \pm 13.5 \mu$, respectively. Five cryoprotectants (CPAs), dimethyl sulfoxide (DMSO), glycerol, ethylene glycol (EG), propylene glycol (PG), and methanol, were tested at the concentrations of 1.5, 2.0 and 2.5 M. After larvae suspended in CPAs, cryoprotectants were loaded in 0.5 ml straws at a larval density of 50-100 larvae per straw, and epuilibrated for 10 and 20 minute at room temperature ($23^{\circ}C$), repectively. Straws were cooled at a rate of $1^{\circ}C$/min from $0^{\circ}C$ to $-12^{\circ}C$, held for 5 min at $-12^{\circ}C$, and then cooled at $2^{\circ}C$/min to $-35^{\circ}C$ and equilibrated for 5 min followed by plunging in liquid nitrogen. After storage in liquid nitrogen for 1 day, straws were thawed in a $30^{\circ}C$ water. As soon as straws were observed to melt, larvae were diluted with an equal volume of ASW and then washed twice with a large volume of ASW at an interval of 2 min to unload the CPAs. The results showed that after equilibration for 10 and 20 minute at room temperature, no larvae survived using methanol as CPAs, and it was observed that larval shells all open slightly, and larval flesh broke down and slopped over the shells. The highest survival rates (D-shaped larvae: 77.6%, umbo larvae: 59.3%) were obtained with 2M DMSO, and 1.5M glycerol yielded survival rates of 53.8% for D-shaped larvae and 37.5% for umbo larvae. The surviving D-shaped larvae showed active rotary motion and perfect membrane integrity and cytoplasmic normality, and the vigorous movement of veliger cilia was observed inside the closed shells. The breakdown of tissue occurred in the abnormal larvae, and the isolated cell often run out of shells.

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Estimation of the Mechanical Properties of the Concrete Tunnel Lining by Drilling Resistance Test (천공저항시험에 의한 콘크리트 터널라이닝의 역학적 특성 추정)

  • Choi, Soon-Wook;Sung, Yun-Chang;Cheong, Ho-Seop;Chang, Soo-Ho
    • Journal of the Korean Geotechnical Society
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    • v.23 no.11
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    • pp.87-98
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    • 2007
  • For the quick rehabilitation of a fire-damaged tunnel structure, it is the most important procedure to investigate the fire-induced damaged zone rapidly. This study aims to propose a new drilling resistance testing method by which mechanical properties of tunnel concrete lining altered by high temperature can be estimated easily and continuously. Especially, it alms to derive the relationships to estimate mechanical properties of mortar and concrete materials from drilling parameters. To obtain the optimum testing condition, a series of drilling resistance tests were carried out for mortar specimens. When the rotation per minute of drill bit, tile penetration rate and the bit diameter were 1,300 rpm, 1.40 mm/sec, and 10 mm respectively, the deviation of measured drilling resistance forces was minimal. Under the optimum testing condition, the relationships between drilling resistance and mechanical properties of mortar specimens were shown to be very favorable. The concept of replacing a mean value of resistance farces measured during drilling with the resistance energy was proposed to consider the effects of randomly distributed aggregates inside a concrete material on drilling resistance. When the concept was applied to concrete materials, a favorable relationship between actual compressive strength and drilling resistance energy was also successfully derived.

Restrained Effect of End Plate on Plane Strain Test Evaluated by Digital Image Correlation Method (디지털 이미지 코릴레이션 기법으로 평가한 평면변형률 시험의 단부 구속 효과)

  • Jang, Eui-Ryong;Choo, Yoon-Sik;Lee, Won-Taeg;Chung, Choong-Ki
    • Journal of the Korean Geotechnical Society
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    • v.24 no.7
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    • pp.25-36
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    • 2008
  • The plane strain test can reproduce the real field condition and failure behavior precisely over other laboratory shear tests. Accordingly, this test has been utilized to investigate the shearing behaviors associated with overall failure behavior and local deformation of soils. However, most plane strain tests have been carried out with restrained end plates due to difficulties in manufacturing the equipment and also performing it. This restraint induces different results with real field because of shear stress on end plates. In this study, plane strain tests with/without bottom plate restraint were performed on Jumunjin-sand. The measurement of overall and local deformation was accomplished by digital image correlation technique as well as external LVDT. By applying digital image correlation method using two consecutive images captured through the transparent wall, local deformation behavior of various parts inside the specimen was estimated. And the formation and development of shear band caused by the restrained effect of end plate and the deformation mechanism of sand under plane strain condition were examined.

An Experimental Study on the Load-settlement Behavior and Settlement-reducing Effect of the Disconnected Piled Raft Foundation (말뚝보강기초의 하중-침하량 거동 및 침하감소효과에 대한 실험적 연구)

  • Lee Yeong-Saeng;Hong Seung-Hyeun
    • Journal of the Korean Geotechnical Society
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    • v.22 no.4
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    • pp.95-104
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    • 2006
  • For the interests in the economical and safe design of foundation system, the concern on the piled raft or disconnected piled raft foundation system is increasing now. In this study, the behavior and the effects of the disconnected piled raft foundation not studied actively in this country were examined using the triaxial compression tests in place of laboratory model tests. The triaxial test samples were prepared with Jumunjin standard sand and the carbon rods, which simulate the ground soil and piles respectively. After the sample in which carbon rods were arranged was laid inside the triaxial chamber, the confining pressure was applied and then loading test was conducted. To analyze the reinforcing effects of the disconnected piled raft foundation, a few number of tests were carried out by changing the number, the diameter and the length of the model piles. As a result of this study, in the disconnected piled raft foundation system, even though the number of pile is few and the diameter of pile is small, the settlement of the foundation system decreased greatly.

A Study on the Production Techniques and Raw Material Characteristics of Clay Bodhisattva Excavated from the Neungsan-ri Temple Site Using CT (CT 조사를 통한 부여 능산리사지 출토 소조보살상의 제작 기법과 재료적 특성 연구)

  • Shin Yeonhong;Ro Jihyun;Kim Jiho;Park Haksoo
    • Conservation Science in Museum
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    • v.29
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    • pp.153-162
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    • 2023
  • Clay figure of Baekje, produced by forming and molding various shapes using clay, are mainly excavated from temple sites and provide essential information for studying the Buddhist art of Baekje. Research on clay figures of Baekje primarily focuses on the characteristics of Baekje-era temples in which such figures are excavated, as well as the role and production techniques of clay figures, by comparing regional and morphological characteristics. In particular, research on the manufacturing method of clay figures is mainly carried out by visual observation, whereas precise scientific analysis is required to understand production techniques and characteristics of raw materials in greater detail. In this study, to confirm such production techniques and material characteristics, computed tomography (CT) scans were conducted on the Clay Bodhisattva excavated from the Neungsan-ri Temple site in Buyeo. As a result, it was found that the Clay Bodhisattva was made using a cylindrical core of fine clay, tied together with several thin branches or reeds with straws. The clay used in the figure bore traces indicating the presence of herbaceous plants, which increase adhesion between clay and prevent cracks in the contraction process. On the other hand, the density of the fine clay differs on the inside and outside of the clay figure. Based on this, it is presumed that the clay was applied around the cylindrical core to shape the Clay Bodhisattva. The clay was reapplied on top of the figure to express the detailed shape and pattern.

A Study on the Contents to Vitalize the Space for Making Traditional Gwangheemun A Tourism Resource (문화유산 광희문(光熙門)의 관광자원화를 위한 공간 활성화 콘텐츠 연구)

  • Kim, Ji Eun;Park, Eun Soo
    • Korea Science and Art Forum
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    • v.23
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    • pp.95-109
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    • 2016
  • The background and objective of this study are as follows. Gwangheemun, one of the 4 small gates of Seoul Castle is a space to represent ordinary people and it is a valuable cultural heritage that shows the process of technical transition of fortification technology during Chosun Dynasty. It is a place that we can expect to play a role as a field where history and culture mix and communicate together. But currently, the environment and facilities around Gwangheemun have fallen behind and become old, so they need to be reorganized as their local feature is not shown distinctly. We need to vitalize the new traditional space that shows local feature. This study has drawn out the method, contents and the result of study like as follows. This study aims to establish an identity based on the historical and cultural backgrounds and suggest the contents to vitalize the space of Gwangheemun as a traditional cultural heritage. By this, this study aims to create a historical and cultural space where people can enjoy, eat and look around. Therefore, based on the historical and cultural feature, it gives an identity as moonlight street, and it has developed and suggested 5 contents to vitalize space: Gwangheemun maintenance, plan, castle restoration plan, village inside the castle, village outside the castle and fashion art street. Contents to vitalize space has a meaning as a specific developmen method of urban restoration, and we can expect to be used as a direction to develop the area to enhance the cultural quality of life of both inhabitants and visitors by forming the brand identity of surrounding area with traditional cultural heritage.

Hierarchical Circuit Visualization for Large-Scale Quantum Computing (대규모 양자컴퓨팅 회로에 대한 계층적 시각화 기법)

  • Kim, JuHwan;Choi, Byung-Soo;Jo, Dongsik
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2021.05a
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    • pp.611-613
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    • 2021
  • Recently, research and development of quantum computers, which exceed the limits of classical computers, have been actively carried out in various fields. Quantum computers, which use quantum mechanics principles in a way different from the electrical signal processing of classical computers, have various quantum mechanical phenomena such as quantum superposition and quantum entanglement. It goes through a very complicated calculation process compared to the calculation of a classical computer for performing an operation using its characteristics. In order to utilize each element efficiently and accurately, it is necessary to visualize the data before driving the actual quantum computer and perform error verification, optimization, reliability, and verification. However, when visualizing all the data of various elements configured inside the quantum computer, it is difficult to intuitively grasp the necessary data, so it is necessary to visualize the data selectively. In this paper, we visualize the data of various elements that make up a quantum computer, and hierarchically visualize the internal circuit components of a quantum computer that are complicatedly configured so that the data can be observed and utilized intuitively.

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Interpretation of Making Techniques through Surface Characteristic Analysis and Non-destructive Diagnosis for the Gilt-bronze Seated Buddha in Dangjin Sinamsa Temple, Korea (당진 신암사 금동여래좌상의 표면특성 분석과 비파괴 정밀진단을 통한 제작기술 해석)

  • CHOI Ilkyu ;YANG Hyeri ;HAN Duru;LEE Chan Hee
    • Korean Journal of Heritage: History & Science
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    • v.56 no.1
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    • pp.100-116
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    • 2023
  • The Sinamsa Temple was built in the late Goryeo Dynasty and a gilt-bronze seated Buddha is enshrined in Geungnakjeon hall in the precinct. Various damages occurred in the gilt layer of the Buddha, such as peeling of the gilt layer and deteriorating gloss. In the study, the conservation conditions of the inside and outside on the statue were accurately investigated, and the making technique was interpreted through the material characteristics and non-destructive diagnosis of the statue. As a result, it is estimated that gold-gilding layer is pure gold, coloration pigment of black is carbon, green is malachite, atacamite and verdigris, red is red lead and cinnabar, respectively. In the deterioration evaluation, peeling, cracking, break out and exfoliation of the gilt layer are confirmed as damages, but the conservation condition is relatively wholesome. However, the gloss of the gilt layer is calculated to be wider in the poorer part than the maintenance part. The ultrasonic velocity of the statue was calculated to be 1,230 to 3,987 (mean 2,608) m/s and showed a relatively wide range. In infrared thermography, peeling was not confirmed, and no special bonding marks were found. In endoscope, some biological damage and corrosion were observed on the surface of the internal metal, and sealed artifacts were identified. Manufacturing technique based on the study, it is considered that the gilt-bronze seated Buddha was cast at once, and the mold was inverted to inject molten metal.

Characteristics of the Expanded Road Embankment Constructed by Lightweight Air-Mixed Soils for a Short-Term (경랑기포혼합토로 단기간에 시공된 확폭도로성토체의 특성)

  • Hwang, Joong Ho;Ahn, Young Kyun;Lee, Young-Jun;Kim, Tae-Hyung
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.4D
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    • pp.377-386
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    • 2010
  • This study was conducted to find out the characteristics of the expanded road embankment constructed by the lightweight air-mixed soil (slurry density $10kN/m^3$) for a short-term without any ground improvement. Compression strength, capillary rise height of the lightweight air-mixed soil and settlement behavior of soft ground were studied. Compression strengths of the specimens sampled at the site after 1 and 5 months of construction were all satisfied the required strength 500 kPa. However, it was not convinced the homogeneity construction, because the values of strength were depending on the sampled location. Also, strength difference between laboratory and site specimens were found about 19%, and thus it should be considered for mixing design. Capillary rise reached about 20 cm for 70 hours because of a numerous tiny pores existed inside the lightweight air-mixed soil. Relationship between settlement and time of the soft ground placed underneath the expanded embankment was estimated by using the measured data and back analysis technique. The current average consolidation ratio and the final settlement after 120 months later were estimated about 32% and 4.5cm, respectively. This settlement is much less value than the allowable settlement 10cm for this structure.

Factor Analysis on Psychological Cause of Speed Reduction in Expressway Tunnel Section Utilizing Importance-Performance Analysis (IPA) (고속도로 터널부 속도 감소에 관한 심리적 요인분석)

  • Lee, Ki Young;Kim, Tae Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.2D
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    • pp.127-134
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    • 2010
  • Tunnel sections on the highway are different from rest of sections on the highway in terms of velocity, the number of passing cars, and vehicle density which, in particular, affect drivers' behavior before and after drivers pass through the tunnel. However, literature review reveals that former studies are too focused on quantitative indicator to consider qualitative aspects. Therefore, this paper conducts survey questionnaire and IPA (Importance Performance Analysis) to find out qualitative improvements on velocity drop on the tunnel sections. The results show as follows: First, drivers require improvements of tunnel form (length and curved form inside tunnel) which is derived from long distance tunnel. Second, experts primarily ask for amendment of geometric characteristics. With comparison of requirements of both drivers and experts, there are many differences in length of tunnel and form of curved tunnel. This also presents that drivers don't satisfy with both length of tunnel and form of curved tunnel that are provided as a part of highway design factors.