• Title/Summary/Keyword: Insect-insect interaction

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Interactions between Insect Species Feeding on Rumex obtusifolius: the Effect of Philaenus spumarius Feeding on the Ecology of Gastrophysa viridula

  • Kwon, Oh-Seok;Nam, Sang-Ho
    • The Korean Journal of Ecology
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    • v.23 no.2
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    • pp.175-180
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    • 2000
  • In order to study the insect-insect interaction of the insect community associated with Rumex obtusifolius. this experiment was designed in such a way that the feeding of one insect could indirectly affect the subsequent insect species through the changes in host plant (plant mediated insect-insect interaction ). Philaenus spumarius and Gastrophysa viridula were selected for the experiment. To investigate the effect of P. spumarius feeding on the ecology of G. viridula, first, statistical analyses were carried out. As results. no significant difference between Control and Experimental was found in the development patterns (Repeated Measures ANOVA, F=0.744, p=0.667) and survivorships (F=0.373. p=0.990). As the results from this experiment show, there was no effect on the ecology of G. viridula due to the previous feeding by P. spumarius on R. obtusifolius leaves.

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Insect Damage to Plants and Structure of Ecological Community: Indirect Interaction Mediated by Insect Damaged Plants (곤충의 가해와 생물군집 구조- 피해식물이 개재된 간접적 상호관계를 중심으로 -)

  • Hyun, Jai-sun
    • Korean journal of applied entomology
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    • v.54 no.2
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    • pp.137-144
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    • 2015
  • Plants can affect adversely the interaction among herbivores by inducing insect resistance chemicals and change of attack behavior of natural enemies. Also, plants may induce favorable effects to herbivores by production of allelochemicals, nutritional variation, or morphological changes. In this review, we examined the effects of the interaction among herbivores mediated by plants and plant-response induced by insect attack, or the life history strategies of insects on the community structure of herbivore insects, and discussed its ecological significance in community level.

Evaluation of Gelation Properties of Salt-Soluble Proteins Extracted from Protaetia brevitarsis Larvae and Tenebrio molitor Larvae and Application to Pork Myofibrillar Protein Gel System

  • Ji Seon Choi;Geon Ho Kim;Ha Eun Kim;Min Jae Kim;Koo Bok Chin
    • Food Science of Animal Resources
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    • v.43 no.6
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    • pp.1031-1043
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    • 2023
  • The purpose of this study was to investigate the functional properties of salt-soluble proteins obtained from Protaetia brevitarsis (PB) and Tenebrio molitor (TM) larvae, the interaction between these proteins and pork myofibrillar protein (MP) in a gel system. The gel properties of salt-soluble protein extracts showed that the PB had a higher viscosity than the TM protein. However, the TM protein had higher gel strength compared with the PB protein. The gelation characteristics of the pork MP gel systems added with lyophilized insect salt-soluble protein powder showed to decrease slightly viscosity compared with MP alone. Adding the TM or PB protein powder did not affect the pork MP's hydrophobicity and sulfhydryl group levels. Furthermore, the protein bands of the MP did not change with the type or amount of insect salt-soluble protein. The cooking yields of the pork MP gels containing PB or TM protein powder were higher than those without insect protein. Regardless of the type of insect salt-soluble protein added, the pork MP's gel strength decreased. Furthermore, as the level of insect powder increased, the surface protein structure became rough and porous. The results demonstrated that proteins extracted from PB and TM larvae interfered with the gelation of pork MP in a gel system.

Landscape Structure Analysis Based on Insect Spatial Distribution in Rural Area (곤충류 공간 분포를 활용한 농경지 경관구조 분석)

  • Lee, Dong-Kun;Yoon, Eun-Joo;Bae, Jung-Hoon
    • Journal of Korean Society of Rural Planning
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    • v.14 no.1
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    • pp.23-32
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    • 2008
  • Landscape structure is important to understand a complex patterns and interaction with adjacent habitat in rural area. The aim of this study is to analyze relationship between landscape structure and insect spatial distribution in rural area to suggest applicable possibility of landscape structure as biological indicator. For this purpose, first, four landscape structure criteria such as distance from the forest; density of farmland-forest ecotone; landscape continuity; and field size are selected. Secondly, these criteria are applied to Gangsang-myeon, Yangpyeong-gun where mosaic feature are conserved at various spatial scale. Thirdly, application of landscape structure criteria is verified using correlation with species number, species diversity, and species richness of insect. As a result, it could be suggested that the landscape structure criteria are useful for explaining insect spatial distribution.

Mass Spectrometry-Based Screening Platform Reveals Orco Interactome in Drosophila melanogaster

  • Yu, Kate E.;Kim, Do-Hyoung;Kim, Yong-In;Jones, Walton D.;Lee, J. Eugene
    • Molecules and Cells
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    • v.41 no.2
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    • pp.150-159
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    • 2018
  • Animals use their odorant receptors to receive chemical information from the environment. Insect odorant receptors differ from the G protein-coupled odorant receptors in vertebrates and nematodes, and very little is known about their protein-protein interactions. Here, we introduce a mass spectrometric platform designed for the large-scale analysis of insect odorant receptor protein-protein interactions. Using this platform, we obtained the first Orco interactome from Drosophila melanogaster. From a total of 1,186 identified proteins, we narrowed the interaction candidates to 226, of which only two-thirds have been named. These candidates include the known olfactory proteins Or92a and Obp51a. Around 90% of the proteins having published names likely function inside the cell, and nearly half of these intracellular proteins are associated with the endomembrane system. In a basic loss-of-function electrophysiological screen, we found that the disruption of eight (i.e., Rab5, CG32795, Mpcp, Tom70, Vir-1, CG30427, Eaat1, and CG2781) of 28 randomly selected candidates affects olfactory responses in vivo. Thus, because this Orco interactome includes physiologically meaningful candidates, we anticipate that our platform will help guide further research on the molecular mechanisms of the insect odorant receptor family.

Parasporin-4, A Novel Cancer Cell-killing Protein Produced by Bacillus thuringiensis

  • Inouye, Kuniyo;Okumura, Shiro;Mizuki, Eiichi
    • Food Science and Biotechnology
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    • v.17 no.2
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    • pp.219-227
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    • 2008
  • Bacillus thuringiensis was isolated as a pathogen of the sotto disease of silkmoth larvae about a hundred years ago. Since then, this bacterium has attracted attentions of not only insect pathologists but also many other scientists who are interested in its strong and specific insecticidal activity. This has led to the recent worldwide development of B. thuringiensis-based microbial insecticides and insect-resistant transgenic plants, as well as a landmark discovery of par asp orin, a cancer cell-specific cytotoxin produced by B. thuringiensis. In this review, we describe examination of interaction between inclusion proteins of B. thuringiensis and brush border membrane of insects using a surface plasmon resonance-based biosensor, identification and characterization of parasporin-4, the latest parasporin produced by the B. thuringiensis A1470 strain, and an effective method for preparing the parasporin-4 from inclusion bodies expressed in the recombinant Escherichia coli cells.

Expression of Recombinant Human Bone morphogenetic protein 2 (hBMP2) in Insect cells

  • Kim, Seong-Wan;Kim, Seong-Ryul;Park, Seung Won;Goo, Tae-Won;Choi, Kwang-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.34 no.1
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    • pp.1-5
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    • 2017
  • Bone morphogenetic protein 2 (BMP2) plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. It is involved also in cardiac cell differentiation and epithelial to mesenchymal transition. In this study, We expressed human BMP2 (hBMP2) recombinant protein using Baculovirus Expression Vector System (BEVS) in Sf9 insect cells. The hBMP2 cDNA was cloned into baculovirus transfer vector, pBacgus-4x-1 and recombinant baculovirus was screened out through X-gal and GUS-fusions assay. Western blot analysis shown that molecular weight of hBMP2 recombinant protein was about 44.71 kDa.

NUMERICAL STUDY ON THE UNSTEADY FLOW PHYSICS OF INSTECTS' FLAPPING FLIGHT USING FLUID-STRUCTURE INTERACTION (FSI를 활용한 2차원 곤충날개 주위 유동장 해석)

  • Lee, K.B.;Kim, J.H.;Kim, C.
    • 한국전산유체공학회:학술대회논문집
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    • 2009.11a
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    • pp.151-158
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    • 2009
  • To implement the insects' flapping flight for developing flapping MAVs(micro air vehicles), the unsteady flow characteristics of the insects' forward flight is investigated. In this paper, two-dimensional FSI(Fluid-Structure Interaction) simulations are conducted to examine realistic flow features of insects' flapping flight and to examine the flexibility effects of the insect's wing. The unsteady incompressible Navier-Stokes equations with an artificial compressibility method are implemented as the fluid module while the dynamic finite element equations using a direct integration method are employed as the solid module. In order to exchange physical information to each module, the common refinement method is employed as the data transfer method. Also, a simple and efficient dynamic grid deformation technique based on Delaunay graph mapping is used to deform computational grids. Compared to the earlier researches of two-dimensional rigid wing simulations, key physical phenomena and flow patterns such as vortex pairing and vortex staying can still be observed. For example, lift is mainly generated during downstroke motion by high effective angle of attack caused by translation and lagging motion. A large amount of thrust is generated abruptly at the end of upstroke motion. However, the quantitative aspect of flow field is somewhat different. A flexible wing generates more thrust but less lift than a rigid wing. This is because the net force acting on wing surface is split into two directions due to structural flexibility. As a consequence, thrust and propulsive efficiency was enhanced considerably compared to a rigid wing. From these numerical simulations, it is seen that the wing flexibility yields a significant impact on aerodynamic characteristics.

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