• Molecules and Cells
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• v.26 no.2
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• pp.146-151
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• 2008

The brown planthopper (BPH) is a major insect pest in rice, and damages these plants by sucking phloem-sap and transmitting viral diseases. Many BPH resistance genes have been identified in indica varieties and wild rice accessions, but none has yet been cloned. In the present study we report fine mapping of the region containing the Bph1 locus, which enabled us to perform marker-aided selection (MAS). We used 273 F8 recombinant inbred lines (RILs) derived from a cross between Cheongcheongbyeo, an indica type variety harboring Bph1 from Mudgo, and Hwayeongbyeo, a BPH susceptible japonica variety. By random amplification of polymorphic DNA (RAPD) analysis using 656 random 10-mer primers, three RAPD markers (OPH09, OPA10 and OPA15) linked to Bph1 were identified and converted to SCAR (sequence characterized amplified region) markers. These markers were found to be contained in two BAC clones derived from chromosome 12: OPH09 on OSJNBa0011B18, and both OPA10 and OPA15 on OSJNBa0040E10. By sequence analysis of ten additional BAC clones evenly distributed between OSJNBa0011B18 and OSJNBa0040E10, we developed 15 STS markers. Of these, pBPH4 and pBPH14 flanked Bph1 at distances of 0.2 cM and 0.8 cM, respectively. The STS markers pBPH9, pBPH19, pBPH20, and pBPH21 co-segregated with Bph1. These markers were shown to be very useful for marker-assisted selection (MAS) in breeding populations of 32 F6 RILs from a cross between Andabyeo and IR71190, and 32 F5 RILs from a cross between Andabyeo and Suwon452.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.297-302
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• 2002

This study was carried out to compare the agronomic characteristics, forage yield, and quality of four sorghum $\times$ sudangrass hybrids at Yonam College of Agriculture Experimental Livestock Farm, Sunghwan in 2001. The four recommended hybrids used in this study were ‘SXl7’ and ‘877F’(heading type), and ‘TE Evergreen’ and ‘Turbo 9’(headless type). sugar content or heading hybrids(SX17 and 877F) were higher then that of headless hybrids(TE Evergreen and Turbo 9). SX17 had resistance to lodging, while all hybrids had resistance to foliar disease and insect. Dry matter(DM) and total digestible nutrients (TDN) yields of heading hybrids were higher then those of headless hybrids. Among sudangrass hybrids, DM and TDN yields of 877F were the highest(21,007 and 12,276kg/ha). The crude protein of Turbo 9(10.5%) was higher than other hybrids. Neutral detergent fiber(NDF) content of headless hybrids were higher than that of heading hybrids, while acid detergent fiber(ADF) and acid detergent lignin(ADL) contents of headless hybrids were lower than those of heading hybrids. Calculated TDN of headless hybrids were higher than that at heading hybrids. Cellulase digestible of organic matter of dry matter (CDOMD) of headless hybrids also were higher then that of heading hybrids. Results of this experiment indicates that heading hybrid was better than headless hybrid in the agronomic characteristics and forage yield of sorghum $\times$ sudangrass. However heading hybrid was lower than leafless hybrid in quality of sorghum $\times$ sudangrass.

• Korean journal of applied entomology
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• v.52 no.1
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• pp.35-43
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• 2013

All tested Korean populations of the diamondback moth, Plutella xylostella, are known to be resistant especially against pyrethroid insecticides by mutation in its molecular target, para-sodium channel. Moreover, P. xylostella is able to develop resistance against most commercial insecticides. This study was performed to develop an efficient control technique against P. xylostella by a combined treatment of an endoparasitoid wasp, Cotesia plutellae, and a microbial insecticide, Bacillus thuringiensis. To investigate any parasitism preference of C. plutellae against susceptible and resistant P. xylostella, five different populations of P. xylostella were compared in insecticide susceptibilities and parasitism by C. plutellae. These five P. xylostella populations showed a significant variation against three commercial insecticides including pyrethroid, organophosphate, neonicotinoid, and insect growth regulator. However, there were no significant differences among five P. xylostella populations in their parasitic rates by C. plutellae. Moreover, parasitized larvae of P. xylostella showed significantly higher susceptibility to B. thuringiensis. As an immunosuppressive agent, viral ankyrin genes (vankyrins) encoded in C. plutellae were transiently expressed in nonparasitized larvae. Expression of vankyrins significantly enhanced the efficacy of B. thuringiensis against the third instar larvae of P. xylostella. Thus an immunosuppression induced by C. plutellae enhanced the insecticidal efficacy of B. thuringiensis. These results suggest that a combined treatment of C. plutellae and B. thuringiensis may effectively control the insecticide-resistant populations of P. xylostella.

• Journal of Plant Biotechnology
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• v.41 no.3
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• pp.127-133
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• 2014

Four transgenic rice lines harboring insect-resistant gene cry3A showed ideal field performances characterized by high considerable resistance to rice water weevil (Lissorhoptrus oryzophilus Kuschel). In this study, we estimated the insertion number of foreign genes, and analyzed the flanking sequences of T-DNAs in rice genome. As a result, T-DNA of BT12R1 line was inserted in exon region of rice chromosome 10. Two copies of T-DNAs were inserted in line BT12R2. BT12R3 line was analyzed at only left border flanking sequence. BT12R4 line was confirmed one copy of foreign gene insertion at the position 24,516,607 ~ 24,516,636 of rice chromosome 5, accompanied by a deletion of 30 bp known genomic sequences. This intergenic position was confirmed none of expressed gene and any deletion/addition of T-DNA sequence. In conclusion, these molecular data of rice water weevil resistant Bt rice would be used to conduct the biosafety and environment risk assessment for GM crop commercialization.

• The Korean Journal of Pesticide Science
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• v.11 no.3
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• pp.210-215
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• 2007

This study aims to investigate if the properly use of pesticide in Ginseng farmland at Gangwon-do, Korea. Questionnaire included 36 questions such as control method for plant diseases, pests and weeds and pattern of pesticide use was answered by 271 Ginseng farmers lived in 4 cities and 8 counties at Gangwon-do. Ginseng farmers have noxious plant diseases, insects and weeds such as leaf spot, anthracnose, snail, stem-mining fly, horseweed and hairy crabgrass. To control of these, 35% of farmers relied on various type of pesticides and 31.5% of farmers used pesticides mingling with natural enemy, microorganism and organic materials. Farmers selected the pesticide based on the their own experiences or by recommendation of market dealers and neighbors, resulting in the use of inappropriate pesticides in the Ginseng farmland(78.8 % ). They followed standard dosage of the pesticide from Handbook of Pesticide Application(96.3%). They, however, used the same pesticides from 2 to 5 years(44.1 %). This consecutive use of pesticide could be induced resistance. This survey resulted that study for pesticide resistance and systematic educational program for proper use and selection of pesticide to Gingseng farmers should be conducted in farmland at Gangwon-do, Korea.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.295-298
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• 2008

"Dongjin2" is a new japonica rice cultivar developed from the cross between Milyang165 with short culm and lodging resistance and $F_1$ plant of Iksan438, HR14018-B-1-1 and Iksan435 with high palatability at Honam Agricultural Research Institute (HARI), NICS, RDA in 2005. This cultivar has short grain shape and about 143 days growth duration from direct seeding to harvesting under Korean climate condition. The milled kernels of "Dongjin2" is translucent with non-glutinous endosperm. It has about 19.3% of amylose content and better palatability of cooked rice compared with "Nampyeongbyeo". This cultivar shows high resistance reaction to the bacterial blight pathogene race from $K_1$ to $K_3$, blast and stripe virus but susceptible to insect pests. "Dongjin2" yields about 5.71 and 5.74 MT/ha under the wet direct seeding and the transplanting at standard fertilizer level. "Dongjin2" would be adaptable for the southern plain area of Korea.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.645-648
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• 2009

"Deuraechan" is a new japonica rice variety developed and registered by the rice breeding team of Department of Rice and Winter Cereal Crop, NICS, RDA in 2008. This variety was derived from a three way cross Iksan438, YR19105Acp222 and Milyang165. This variety has about 122 days of growth duration from transplanting to harvesting in Honam and Youngnam plain of Korea. It is about 76 cm in culm length and tolerance to lodging. In reaction to biotic stresses, it shows moderately resistance to blast, resistance to bacterial blight pathogen races from $K_1$ to $K_3$, and Rice stripe virus, but susceptible to other major diseases and insect pests. The milled rice of "Deuraechan" has high Quality, midium short grain. The milled rice yield of this variety is about 6.52 MT/ha in local adaptability test for three years. "Deuraechan" would be adaptable to Middle plain, Honam plain and Youngnam plain area of Korea.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.314-317
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• 2008

"Dami" is a new japonica rice variety developed and registered by the rice breeding team of Honam Agricultural Research Institute, NICS, RDA in 2006. This variety was derived from a cross between Iksan438 with lodging tolerance and high yield potential, and Iksan426 This variety has about 122 days of growth duration from transplanting to harvesting in Honam and Youngnam plain of Korea. It is about 83 cm in culm length and tolerance to lodging. In reaction to biotic stresses, it shows moderately resistance to blast, and resistance to bacterial blight pathogen races from $K_1$ to $K_3$, but susceptible to other major diseases and insect pests. The milled rice of "Dami" has high Quality, midium short grain. The milled rice yield of this variety is about 5.92 MT/ha in local adaptability test for three years. "Dami" would be adaptable to Middle plain, Honam plain and Youngnam plain area of Korea.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.452-455
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• 2008

"Baegseolchal" is a new Japonica type waxi rice variety developed and registered by the rice breeding team of Department of Rice and Winter Cereal Crop, NICS, RDA in 2007. This variety was derived from a across between Iksan435 which is highly tolerant to lodging and high yield potential, and Iksan425 which is resistance to multiple diseases. This variety has about 120 days of growth duration from transplanting to harvesting in middle, Honam and Youngnam plain of Korea. It is about 80 cm in culm length and tolerant to lodging. It is moderately resistant to blast, and resistance to bacterial blight pathogen races from $K_1$ to $K_3$, but susceptible to other major diseases and insect pests. "Baegseolchal" is midium-short grain and the milled rice exhibits high whiteness. The milled rice yield was about 5.37 MT/ha in local adaptability test for three years. "Baegseolchal" would be adaptable to plain areas south of the Chungnam Province of Korea.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.522-528
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• 2018

Saemimyeon, a Tongil type, medium-late maturing rice variety, is especially used for preparing rice noodles. Its high amylose content was developed to fit market demands and to be affordable for rice processing industries. One of the high yielding lines, Milyang181 (Hanareum), was used in the final three-way cross of $IR50^*2$/YR18241-B-B-115-1-1 for yield improvement and cultivation stabilization, including disease resistance. YR24235-10-1-3, a high yielding and compact plant type, was selected and named Milyang278 after yield test at NICS (RDA, Miryang) in 2010. It was subjected to regional yield test at six sites in the middle and southern plain areas of South Korea. Saemimyeon heading occurs on August 12 and is a mid-late maturing cultivar, with resistance to leaf blast, rice stripe virus, and bacterial blight (K1-K3a), but it is susceptible to major diseases and insect pest infestation. Saemimyeon showed a high amylose content of 26.7%, with a relatively low KOH digestion value of 3.5, which are key factors in rice noodles and pasta processing. In the local adaptability tests, the yield of Saemimyeon was 7.08 MT/ha-an increase of approximately 106% compared to that of Dasan. Thus, Saemimyeon is suitable for cultivation in the southern and middle plain areas of South Korea.