• Title/Summary/Keyword: Inonotus mikadoi

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Physiological Characteristics and Optimized Culture Condition of the Mycelia of Inonotus mikadoi. (황갈색시루뻔버섯(Inonotus mikadoi)의 균사체 배양 최적 조건 및 생리학적 특성)

  • 최수정;김성준;한영환
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.100-103
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    • 2004
  • The culture condition and medium composition for the enhanced mycelial growth of Inonotus mikadoi IMSNU 32058 were investigated. The optimal temperature and pH for the mycelial growth were $27^{\circ}C$ and pH 4.5, respectively. Among the complex media tested, the malt extract medium and Phellinus igniarius medium were very good for mycelial growth of I. mikadoi. When Czapek-Dox medium was used as a minimal medium for cultivation of mycelia, xylose, raffinose and carboxymethyl cellulose were very excellent as a carbon and energy source. With respect to carbohydrate, sucrose and glucose were very good carbon sources. In general, organic complex nitrogen sources were better than other inorganic ones. As the organic complex nitrogen sources tested, yeast extract, soytone, proteose peptone and bacto peptone were the best as a source of organic nitrogen. When ammonium sulfate as an inorganic source of nitrogen was used, the enhanced mycelial growth was shown. p-Aminobenzoic acid was proved to be most appropriate source of vitamin. After the mycelia of I. mikadoi was cultivated at $27^{\circ}C$ for 5 days in MEM broth (pH 4.5), the activities of both exomycelial and endo-mycelial enzymes were determined. Among endomycelial enzymes assayed, the specific activity of laccase was much higher than those of other enzymes. When the fungus was grown in MEM broth, exomycelial specific enzyme activity of laccase was comparatively high. However, little or no enzyme activities of protease, chitinase and lipase were found.

First Record of the Fungivorous Tenebrionid, Bolitotrogus kurosonis Miyatake (Coleoptera: Tenebrionidae), from Korea with a New Host Fungus (한국산 균식성 미기록종, 갈색가시거저리의 분류학적 검토와 숙주버섯)

  • Jung, Boo-Hee;Lee, Jong-Wook
    • Korean journal of applied entomology
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    • v.50 no.4
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    • pp.279-282
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    • 2011
  • A taxonomic review of Bolitotrogus kurosonis Miyatake 1964 is presented. B. kurosonis is a fungivorous tenebrionid beetle and is a rare inhabitant of fungi on deciduous trees (Quercus, Carpinus laxiflora, etc.) in Korea. Sporophores of Inonotus mikadoi (Lloyd) Imaz. was the obligate feeding and breeding sites for this species in Jeju-do. A description, an additional key to the Korean Bolitophagini, fungal host, photographs of adult, and illustrations of diagnostic characteristics are provided.

Taxonomical Studies on Korean Higher Fungi for the Publication of Colored Illustrations (원색도감(原色圖鑑) 발간(發刊)을 위한 한국산(韓國産) 고등균류(高等菌類)의 분류학적(分類學約) 연구(硏究))

  • Lee, Ji-Yul
    • The Korean Journal of Mycology
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    • v.9 no.2
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    • pp.77-91
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    • 1981
  • This study has been carried out for the publication of colored illustrations of Korean fungi. The fresh higher fungi were collected and photographed, for the most part, at Gwangneung, Kyonggi Province, Mt. Yongmun in Kyonggi Province, Mt. Sokri in Chungcheong Province, Mt. Jiyee in Kyeongsang Province, Mt. Mudeug in Jeonra Province, and Mt. Hanra in Jeuju Island from September 1, 1980 to August 31, 1981. These higher fungi were made colored slides and dried specimens, then classified. According to the investigated result, colored slides and classification for the common 151 species were completed among 620 spp. which were known all of the fungi in Korea. They were included 2 classes, 37 families, 89 genera, 149 species, 1 variety and 1 forma. Their world distributions were investigated. Fifteen species of them, that is, Aleuria aurantia (Fr.) Fuckel, Patella scutellata (St. Amans) Lambotte, Calocora viscosa Fr., Hygrocybe turunda (Fr.) Karst, Suillus pictus (Peck) Smith et Thiers, Marasmius purpureostriatus Hongo, Pleurocybella porrigens (Fr.) Sing., Lepiota bresadoiae Schulzer, Russula aurata Fr., Lactarius hygrophoroides Berk. et Br., Thelephora multipartita Fr., Inonotus mikadoi (Lloyd) Imaz, Antrodia mollis (Sommerf.) Karst, Ganoderma neo-japonicum Imaz. and Tyromyces lacteus (Fr.) Murr. were found to be new in Korea. In addition their common names and descriptons were prepared. According to study plan, this paper pressed only one page in colored plate among 151 species.

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Lipoxygenase Inhibitory Activity of Korean Indigenous Mushroom Extracts and Isolation of an Active Compound from Phellinus baumii

  • Lee, Seung Woong;Song, Ja-Gyeong;Hwang, Byung Soon;Kim, Dae-Won;Lee, Yoon-Ju;Woo, E-Eum;Kim, Ji-Yul;Lee, In-Kyoung;Yun, Bong-Sik
    • Mycobiology
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    • v.42 no.2
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    • pp.185-188
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    • 2014
  • We investigated a total of 335 samples of Korean native mushroom extracts as part of our lipoxygenase (LOX) inhibitor screening program. Among the mushroom-methanolic extracts we investigated, 35 exhibited an inhibitory activity greater than 30% against LOX at a concentration of 100 ${\mu}g/mL$. Especially, Collybia maculata, Tylopilus neofelleus, Strobilomyces confusus, Phellinus gilvus, P. linteus, P. baumii, and Inonotus mikadoi exhibited relatively potent LOX inhibitory activities of 73.3%, 51.6%, 52.4%, 66.7%, 59.5%, 100.0%, and 85.2%, respectively. Bioassay-guided fractionation led to the isolation of inoscavin A from the methanolic extract of P. baumii, which showed the most potent activity and was identified by spectroscopic methods. Specifically, inoscavin A exhibited potent LOX inhibitory activity with an $IC_{50}$ value of $6.8{\mu}M$.