• Journal of Veterinary Science
• /
• v.21 no.3
• /
• pp.48.1-48.18
• /
• 2020

Background: Mature oocytes at the metaphase II status (MII-stage oocytes) played an important role in assisted reproductive technology in non-human primates. Objectives: In order to improve the proportion of MII-stage oocytes retrieval, three different superovulation protocols were performed on 24 female cynomolgus monkeys. Methods: All the monkeys received once-daily injection of follicle-stimulating hormone (25 international unit [IU]) on day 3 of the menstruation, 3-day intervals, twice daily for 8-12 days until the time of human chorionic gonadotropin (1,500 IU) injection, on the 14-17th day of menstruation collecting oocytes. The difference between protocol I and protocol II was that 0.1 mg the gonadotropin-releasing hormone agonist was injected on day 1 of the menstruation, while the difference between personalized superovulation protocol and protocol II was that oocytes could be collected on the 14-17th day of menstrual cycle according to the length of each monkey. Results: The total number of oocytes harvested using the personalized superovulation protocol was much higher than that using protocol I (p < 0.05), and the proportion of MII-stage oocytes was significantly greater than that from either superovulation protocol I or II (p < 0.001 and p < 0.01 respectively), while the proportion of immature oocytes at the germinal vesicle was less than that from superovulation protocol I (p < 0.05). Conclusions: The personalized superovulation protocol could increase the rate of MII-stage oocytes acquired, and successfully develop into embryos after intracytoplasmic sperm injection, and eventually generated fetus.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.21 no.7
• /
• pp.84-90
• /
• 2022

The purpose of the cover housing component of a car door is to protect the terminals of the plug housing that connects the electric control unit on the door side to the car body. Therefore, for a smooth assembly with the plug housing and to prevent contaminants from penetrating into the gaps that occur after assembly, the warpage of the cover housing should be minimized. In this study, to minimize the warpage of the cover housing, optimization was performed for design factors related to the mold and processes based on the injection molding simulation. These design factors include gate location, gate diameter, injection time, resin temperature, mold temperature, and packing pressure. To optimize the design factors, Taguchi's approach to the design of experiments was adopted. The optimal combination of the design factors and levels that minimize warpage was predicted through L₁₈-orthogonal array experiments and main effects analysis. Moreover, the warpage under the optimal design was estimated by the additive model, and it was confirmed through the simulation experiment that the estimated result was quite consistent with the experimental result. Additionally, it was found that the warpage under the optimal design was significantly improved compared to both the warpage under the initial design and the best warpage among the orthogonal array experimental results, which numerically decreased by 36.9% and 23.4%, respectively.

• The Korean Journal of Community Living Science
• /
• v.25 no.4
• /
• pp.557-566
• /
• 2014

This study examines he trend in the development of protective clothing for pesticide spraying based on materials with domestic patents and proposes pesticide protection clothing using a cooling blower unit effective for reducing heat stress in pesticide spraying. There was a total of 54 domestic patents on protective clothing related to pesticide spraying, reflecting a sharp increase based on the increasing demand for protective clothing since 2000. Protective clothing with a lower level of heat stress as the core technology accounted for 35.2% of these patents, and recent years have witnessed the increased development of protective clothing supplying cold air to the interior of the clothing through a separate device. However, this may cause some inconvenience in the activity of farmers. Therefore, this study proposes a lightweight cooling blower unit that does not hinder the user's appearance and activity. In the cooling blower unit, contaminated air from outside is purified through a filter and cools down as it passes a cooling device with refrigerant in the copper pipe. This chilled air is supplied to the interior of the clothing through a bidirectional inhaling blower. The proposed protective clothing is an overall with raglan sleeves. Its chill injection site has an area where the most conspicuous change in temperature is selected, and at the back, there is a large pocket for a cooling blower unit.

• Biomedical Science Letters
• /
• v.8 no.2
• /
• pp.71-75
• /
• 2002

Paraquat (N,N'-dimethyl-4,4'-bipyrrimidinium-dichlorides (PQ): MW 186.6) has been used to killing verierty of hubside plants. For this study were use Sprague-dawely rats (190$\pm$10 gm) and injection 40 mg/kg BW of paraquat (LD$_{50}$) to 24 hrs PQ and 4 days PQ group excepte normal control. For the measurement of blood calcium Ino-phosphorus and activity of alkaline phosphatase (ALP) by HITTACH 746. In serum phosphoruse of normal control were 8.0$\pm$1.2 mg/dl and 24 hrs PQ group were 8.9$\pm$1.0 mg/dl (P<0.05) and 4 days PQ group were 8.8$\pm$0.42 gmm/dl (P<0.05). In serum phosphorus were very sensitive uptaked to 10% within only 24 hours, but 4 days of PQ were similar uptake level than 24 hrs PQ. So this 8.9 mg/dl of sem phosphorus may be thought threshold level becouse does not more encrease. In blood calcium normal of rats were measured 9.51$\pm$0.3 mg/dl and 24 hrs of PQ group were 9.9$\pm$0.51 mg/dl (uptaked 4.5%, p>0.05). This uptake cannot fined meaning mathmatic statistics but 4 days PQ groups of calcium were 10.43$\pm$0.37 gm/dl (uptaked 10%, p<0.05). That 24 hrs PQ groups of caclium dose not reacted sensitive to irritated by PQ. So, when use oxidants of PQ, the blood calcium and Ino-phosphorus were linear correlated uptake that reasone thought may be move out form hardness bone tissue to in blood it does not take feeding to hunger for 4 days. In ALP normal of rats were measured 991$\pm$106 unit/L. In 24 hrs irritated PQ rats were fall down by 629$\pm$91 unit/L (P<0.001) but in 4 days of PQ rats were 792$\pm$85 unit/L (P<0.0011) that the activity of level were mild recovered activity from 629 unit (63%) to 792 unit (80%). So, that the reasone of ALP were very sensitive activity and reverse correlated to blood calcium or phosphorus irritated by PQ.

• Child Health Nursing Research
• /
• v.16 no.4
• /
• pp.352-359
• /
• 2010

Purpose: To explore premature infants' pain response to routine procedures in the neonatal intensive care unit (NICU). Methods: The participants were 56 preterm infants who showed 149 pain responses to 8 high frequency routine procedures which were evaluated using the Premature Infant Pain Scale (PIPS). Videotaped recording was used for data collection. Data were analyzed with descriptive analysis, paired t-test, and Pearson's correlation coefficient. Results: PIPS scores for each procedure were as follows; for removal of central catheter dressing, 6.17 (2.04), venous sampling, 6.12 (2.87), intramuscular injection, 6.05 (2.38), insertion of a peripheral line, 5.38 (2.16), insertion of feeding tube, 4.40 (1.34), heel stick, 4.33 (1.23), insertion of central line, 4.00 (2.12), and endotracheal suctioning, 2.90 (1.25). PIPS score was negatively correlated with gestational age (r=-.218, p=.007) and birth weight (r=-.249, p=.002) among general characteristics of the infants. Conclusion: The majority of 8 routine procedures were found to be painful for premature infants in the NICU. Therefore, adequate pain management related to procedures should be provided to premature infant in the NICU.

• Advances in biomechanics and applications
• /
• v.1 no.1
• /
• pp.41-55
• /
• 2014

Acellular intra-myocardial biomaterial injections have been shown to be therapeutically beneficial in inhibiting ventricular remodelling of myocardial infarction (MI). Based on a biventricular canine cardiac geometry, various finite element models were developed that comprised an ischemic (II) or scarred infarct (SDI) in left ventricular (LV) antero-apical region, without and with intra-myocardial biomaterial injectate in layered (L) and bulk (B) distribution. Changes in myocardial properties and LV geometry were implemented corresponding to infarct stage (tissue softening vs. stiffening, infarct thinning, and cavity dilation) and injectate (infarct thickening). The layered and bulk injectate increased ejection fraction of the infarcted LV by 77% (II+L) and 25% (II+B) at the ischemic stage and by 61% (SDI+L) and 63% (SDI+B) at the remodelling stage. The injectates decreased the mean end-systolic myofibre stress in the infarct by 99% (II+L), 97% (II+B), 70% (SDI+L) and 36% (SDI+B). The bulk injectate was slightly more effective in improving LV function at the remodelling stage whereas the layered injectate was superior in functional improvement at ischemic stage and in reduction of wall stress at ischemic and remodelling stage. These findings may stimulate and guide further research towards tailoring acellular biomaterial injectate therapies for MI.

• Research in Vestibular Science
• /
• v.17 no.4
• /
• pp.142-151
• /
• 2018

Objectives: Excitability o medial vestibular nucleus (MVN) in the brainstem can be affected by changes in the arterial blood pressure. Several animal studies have demonstrated that acute hypotension results in the alteration of multiunit activities and expression of cFos protein in the MVN. In the field of extracellular electrophysiological recording, tetrode technology and spike sorting algorithms can easily identify single unit activity from multiunit activities in the brain. However, detailed properties of electrophysiological changes in single unit of the MVN during acute hypotension have been unknown. Methods: Therefore, we applied tetrode techniques and electrophysiological characterization methods to know the effect of acute hypotension on single unit activities of the MVN of rats. Results: Two or 3 types of unit could be classified according to the morphology of spikes and firing properties of neurons. Acute hypotension elicited 4 types of changes in spontaneous firing of single unit in the MVN. Most of these neurons showed excitatory responses for about within 1 minute after the induction of acute hypotension and then returned to the baseline activity 10 minutes after the injection of sodium nitroprusside. There was also gradual increase in spontaneous firing in some units. In contrast small proportion of units showed rapid reduction of firing rate just after acute hypotension. Conclusions: Therefore, application of tetrode technology and spike sorting algorithms is another method for the monitoring of electrical activity of vestibular nuclear during acute hypotension.

• Journal of Korean Neurosurgical Society
• /
• v.66 no.4
• /
• pp.400-408
• /
• 2023

Objective : Nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2) is a crucial factor for the survival of neuron. The role of NMNAT2 in damage following traumatic brain injury (TBI) remains unknown. This study was designed to investigate the role of NMNAT2 in TBI-induced neuronal degeneration and neurological deficits in rats. Methods : The TBI model was established in Sprague-Dawley rats by a weight-dropping method. Real-time polymerase chain reaction, western blot, immunofluorescence, Fluoro-Jade C staining, and neurological score analyses were carried out. Results : NMNAT2 mRNA and protein levels were increased in the injured-side cortex at 6 hours and peaked 12 hours after TBI. Knocking down NMNAT2 with an injection of small interfering RNA in lateral ventricle significantly exacerbated neuronal degeneration and neurological deficits after TBI, which were accompanied by increased expression of BCL-2-associated X protein (Bax). Conclusion : NMNAT2 expression is increased and NMNAT2 exhibits neuroprotective activity in the early stages after TBI, and Bax signaling pathway may be involved in the process. Thus, NMNAT2 is likely to be an important target to prevent secondary damage following TBI.

• Archives of Plastic Surgery
• /
• v.41 no.6
• /
• pp.620-629
• /
• 2014

Keloid scars are often considered aesthetically unattractive and frustrating problems that occur following injuries. They cause functional and cosmetic deformities, displeasure, itching, pain, and psychological stress and possibly affect joint movement. The combination of these factors ultimately results in a compromised quality of life and diminished functional performance. Various methods have been implemented to improve keloid scars using both surgical and non-surgical approaches. However, it has proven to be a challenge to identify a universal treatment that can deliver optimal results for all types of scars. Through a PubMed search, we explored most of the literature that is available about the intralesional injection treatment of hypertrophic scars and keloids and highlights both current (corticosteroid, 5-fluorouracil, bleomycin, interferon, cryotherapy and verapamil) and future treatments (interleukin-10 and botulinum toxin type A). The reference lists of retrieved articles were also analysed. Information was gathered about the mechanism of each injection treatment, its benefits and associated adverse reactions, and possible strategies to address adverse reactions to provide reliable guidelines for determining the optimal treatment for particular types of keloid scars. This article will benefit practitioners by outlining evidence-based treatment strategies using intralesional injections for patients with hypertrophic scars and keloids.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.8 no.3
• /
• pp.163-170
• /
• 2000

Accident statistics shows that the portion of fatal occupant injuries due to side impacts is considerably high. The side impact usually leads to a severe intrusion of side structure into the passenger compartment. Furthermore, the safety zone for the side impact is relatively small compared to the front impact. Those kinds of physics for side impact frequently result in a fatal injury for the occupant. Therefore, NHTSA and EEVC are trying to intensify the regulation for the occupant protection against side impact. Both the regulation and recent market trends are asking for an installation of side airbag. There are several types of system configuration for side impact sensing. In this paper, we adopt the acceleration-based remote sensing method for the side airbag control system. We mainly focus on the development of hardware and crash discrimination algorithm of remote sensing unit. The crash discrimination algorithm needs fast decision of airbag firing especially for high-speed side impact such as FMVSS 214 and EEVC tests. It is also required to distinguish between low-speed fire and no-fire events. The algorithm should have a sufficient safety margin against any misuse situation such as hammer blow, door slam, etc. This paper introduces several firing criteria such as acceleration. velocity and energy criteria that use physical value proportional to crash severity. We have made a simulation program by using Matlab/Simulink to implement the proposed algorithm. We have conducted an algorithm calibration by using real crash data for 2,500cc vehicle. The crash performance obtained by the simulation was verified through a pulse injection method. It turned out that the results satisfied the system requirements well.