• Journal of Microbiology and Biotechnology
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• v.24 no.1
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• pp.59-69
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• 2014

One of the major challenges in metabolic engineering for enhanced synthesis of value-added chemicals is to design and develop new strains that can be translated into well-controlled fermentation processes using bioreactors. The aim of this study was to assess the influence of various fed-batch strategies in the performance of metabolically engineered Pseudomonas putida strains, ${\Delta}gcd$ and ${\Delta}gcd-pgl$, for improving production of medium-chain-length polyhydroxyalkanoates (mcl-PHAs) using glucose as the only carbon source. First we developed a fed-batch process that comprised an initial phase of biomass accumulation based on an exponential feeding carbon-limited strategy. For the mcl-PHA accumulation stage, three induction techniques were tested under nitrogen limitation. The substrate-pulse feeding was more efficient than the constant-feeding approach to promote the accumulation of the desirable product. Nonetheless, the most efficient approach for maximum PHA synthesis was the application of a dissolved-oxygen-stat feeding strategy (DO-stat), where P. putida ${\Delta}gcd$ mutant strain showed a final PHA content and specific PHA productivity of 67% and $0.83g{\cdot}l^{-1}{\cdot}h^{-1}$, respectively. To our knowledge, this mcl-PHA titer is the highest value that has been ever reported using glucose as the sole carbon and energy source. Our results also highlighted the effect of different fed-batch strategies upon the extent of realization of the intended metabolic modification of the mutant strains.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.9 no.3
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• pp.155-164
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• 2009

Digital Content Marketplace based on Interactive Media is defmed as the marketplace for content service between contents supplier and consumer through iDTV environment. This Marketplace is increasing interest to u-Life service with Digital Environment. To Interactive Media, it can contribute to enhance its effectiveness by developing various contents and service model in the initial phase of broadcasting-communication convergence. This study designed metadata using Digital Content marketplace based on Interactive Media. Specially the matadata designing include recommendation-tag for supply supplementary content. It can support self-directed action. Through basic metadata with weight value, it is designed to support supplementary content customer to want on the marketplace. Recommendation-System can be built by many method and to recommend the service content including explicit properties using collaborative filtering method can solve limitations in existing content recommendation.

• Korean Business Review
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• v.11
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• pp.15-38
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• 1998

This study explores the evolution of technical innovation over time. It focuses on sectors of the information technology because this industry can be referred to as one of the most dynamic industries of all times. Following evolutionary theorists, we argue that technological change is gradual and that superior firms and technologies are reward by the' selection' environment. In the initial phase of the industry life cycle, technological change is expected to be radical and uncertainty is high. Over time a product or technology is likely to arise which stands out above all other products or technologies. These so-called 'basic designs' serve as sorts of 'technological guideposts' for further developments in the technology. Once a basic design established, technological progress tends to follow consistent paths or trajectories. The cumulative character of technological progress facilitates a rapid expansion of the boundaries of the technology until the natural limits of the technology are approached and technological progress slows down. Following ecological theories, supply-side developments in the industry are described on the basis of five different organizational types. On the basis of this pattern of market and technological evolution we came up with seven basic propositions.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.49 no.7
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• pp.30-38
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• 2012

This paper presents packet detection, frequency offset estimation architecture and performance analysis for OFDM-based wireless personal area network (WPAN) systems. Packet detection structure is used to find the start point of a packet exactly in WPAN system as the correlation value passes the constant threshold value. The applied autocorrelation structure of the algorithm can be implemented simply compared to conventional packet detection algorithms. The proposed frequency offset estimation architecture is designed for phase rotation process structure, internal bit reduction to reduce hardware size and the frequency offset adjustment block to reduce look-up table size unlike the conventional structure. If the received signal can be compensated by estimated frequency offset through the correction block, it can reduce the impact on the frequency offset. Through the performance result, the proposed structure has lower hardware complexity and gate count compared to the conventional structure. Thus, the proposed structure for OFDM-based WPAN systems can be applied to the initial synchronization process and high-speed low-power WPAN chips.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.34 no.6
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• pp.1837-1844
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• 2014

The study of safety culture estimate has received a wide attentions in many other industries, rather in the contexture of railroad stands at an initial phase. A factor analysis and internal reliability test were conducted to check if the survey results are reliable by looking at how the factors are dimensioned and how the questionnaires are sub-grouped in accordance with such factors, and further if there are any other common attributes being extracted as a new factor. The designed 5 factors were expanded to 7 through the test, and they were renamed accordingly. The questionnaires are reorganized according to the new 7 factors. It implicates that thorough investigation of the common attributes is important in reducing the gaps between interviewers' and interviewees' understanding. Moreover, the results of internal reliability test represent the new design of questionnaire is reasonably acceptable with the high reliable values. We expect such approaches can be applied for the railway culture survey study to be more reliable and applicable.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.6
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• pp.523-535
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• 2012

Solar powered aircraft are becoming more and more interesting for future long endurance missions at hight altitudes, because they could provide surveillance, earth monitoring, telecommunications, etc. without any atmospheric pollution and hopefully in the near future with competitive costs compared with satellites. However, traditional aircraft sizing methods currently employed in the conceptual design phase are not immediately applicable to solar powered aircraft. Hence, energy balance and constraint analyses were performed to determine how various power system components effect the sizing of a solar powered long endurance aircraft. The primary power system components considered in this study were photovoltaic (PV) modules for power generation and regenerative fuel cells for energy storage. To verify current research results, these new sizing methods were applied to HALE aircraft and results were presented.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.47 no.4
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• pp.289-299
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• 2019

Attitude control modeling and simulation (M&S) can be extensively applied in overall development process, from simple algorithm design to on-board software verification. This paper introduces CMG-based attitude control M&S software, which consists of 6-DOF modeling (CMG and space environments modeling), and attitude control algorithm. The M&S software is divided into three modules, from an inner CMG motor control module to an outer earth observation mission module. While an application of this developed software is currently limited to the initial-phase attitude controller development, its application area can be extended to the later-phases by considering sophisticated model information in future.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.18-23
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• 2021

There has been demand for many magnetic sensor applications, and to develop low-cost devices, it is critical to accurately understand the behavior of the magnetic field and the characteristics of magnetic sensors and target devices during initial development phase. The magnetic field has been known to have very complicated nonlinear data to calculate, so it has required expensive computing machines or research to accurately calculate the magnetic sensor values. However, this paper introduces a characteristic of a magnetic sensor called the giant magnetoresistance (GMR) and proposes simple and sufficient approaches to develop a wearable joystick device using a magnetic sensor. Particularly, this paper introduces the design factors for how to properly develop a low-cost wearable joystick device using magnetic sensors after carefully considering the mechanism of a real joystick and the characteristics of magnetic sensors. As a result, user test results are provided to show how users can operate this new wearable joystick device.

• Journal of KIBIM
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• v.12 no.2
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• pp.49-59
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• 2022

The main idea of this study is to propose a BIM-based automation system drawing up a report of energy conservation plan in the architecture division. In order to obtain a building permit, an energy conservation plan must be prepared for buildings with a total floor area of 500m² or more under the current law. Currently, it is adopted as a general method to complete a report by obtaining data and drawings necessary for an energy conservation plan through manual work and input them directly into the verification system. This method takes a lot of effort and time in the design phase which ultimately increases the initial cost of the business, including the services of companies specialized in the environmental field. However, in preparation for mandatory BIM work process in the future, it is necessary to introduce BIM-based automatic creation system that has an advantage for shortening the whole process to enable rapid permission of energy-saving designs for buildings. There may be many methods of automation, but this study introduces how to build an application using Dynamo of Revit, in terms of utilizing BIM, and write an energy conservation plan by automatic completion of report through Dynamo and Excel's VBA algorithm, which can save time and cost in preparing the report of energy conservation plan compared with the manual process. Also we have insisted that the digital transformation of architectural process is a necessary for an efficient use of our automation system in the current energy conservation plan workflow.

• Nuclear Engineering and Technology
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• v.54 no.11
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• pp.4329-4337
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• 2022

Jet impinging device is designed for decay heat removal on horizontal fuel rods in a low temperature heating reactor. An experimental system with a fuel rod simulator is established and experiments are performed to evaluate water film covering capacity, within 0.0287-0.0444 kg/ms mass flow rate, 0-164.1 kW/m² heating flux and 13.8-91.4℃ feeding water temperature. An effective method to obtain the film coverage rate by infrared equipment is proposed. Water film flowing patterns are recoded and the film coverage rates at different circumference angles are measured. It is found the film coverage rate decreases with heating flux during single-phase convection, while increases after onset of nucleate boiling. Besides, film coverage rate is found affected by Marangoni effect and film accelerating effect, and surface wetting is significantly facilitated by bubble behavior. Based on the observed phenomenon and physical mechanism, dry-out depth and initial dry-out rate are proposed to evaluate film covering potential on a heating surface. A model to predict film coverage rate is proposed based on the data. The findings would have reliable guide and important implications for further evaluation and design of decay heat removal system of new reactors, and could be helpful for passive containment cooling research.