• Korean Journal of Acupuncture
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• 제28권1호
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• pp.61-69
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• 2011

Objectives : The purpose of this study is to investigate effects of White Ginseng-Ejung-tang acupuncture solution (EJ) on nitric oxide (NO) and of hydrogen peroxide production in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). Methods : Cell viability was measured by modified MTT assay. NO production was measured by Griess reagent assay. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. Significant differences were examined by using a Student's t-test. Results : The results of the experiment are as follows. 1. EJ did not show cell toxicity against RAW 264.7 cells for 24 hr incubation at the concentrations of up to $200\;{\mu}g$/mL in RAW 264.7 cells. 2. EJ significantly inhibited NO production for 24 hr incubation in RAW 264.7 cells (p <0.05). 3. EJ significantly inhibited the LPS-induced production of NO for 24 hr incubation in RAW 264.7 cells (p <0.05). 4. EJ significantly inhibited the LPS-induced production of hydrogen peroxide for 16, 24, 40, 48, 64, and 72 hr incubation in RAW 264.7 cells (p <0.05). Conclusions : These results suggest that EJ has an anti-inflammtory property related with its inhibition of NO and hydrogen peroxide production in LPS-induced macrophages.

• Preventive Nutrition and Food Science
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• 제15권2호
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• pp.92-97
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• 2010

Luteolin and chicoric acid are the most abundant phytochemicals in dandelion (Taraxacum officinale) leaf. In this study, four kinds of extraction methods [hot water, ambient temperature (AT) water, ethanol, and methanol] were applied to analyze the contents of both phytochemicals and verify their anti-inflammatory and antioxidative activities. The methanol extract showed the most potent nitric oxide (NO) inhibitory effect. The luteolin and chicoric acid concentrations were 3.42 and $12.86\;{\mu}g/g$ dandelion leaf in the methanol extract. The NO-suppressive effect of luteolin and chicoric acid was identified in a dose-dependent manner with $IC_{50}$ values of $21.2\;{\mu}M$ and $283.6\;{\mu}M$, respectively, in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells without cytotoxicity. Malondialdehyde (MDA) concentration, as an index for free radical injury on cell membrane, was also dose-dependently inhibited by the two compounds. The suppressive effect was further examined using mRNA and protein expression levels, which were attributable to the inhibition of inducible nitric oxide synthase (iNOS). These results suggest that two phytochemicals in dandelion leaf, luteolin and chicoric acid, may play an important role in the amelioration of LPS-induced oxidative stress and inflammation.

• 한국응용과학기술학회지
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• 제35권2호
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• pp.325-335
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• 2018

본 연구는 화장품 소재로서 인삼복합물의 가능성을 확인하기 위한 것이다. 이를 위해 우리는 인삼복합물 에탄올 추출물을 사용하여 항염증, 미백 효과에 대한 생물학적 활성 평가를 수행하였다. 시료는 인삼(A), 상황(B), 리기다소나무(C)를 각 70% 에탄올로 추출한 후 (A)1 : (B)1 : (C)0.5의 비율로 혼합한 시료를 사용하였다. 대식세포(RAW 264.7 cells)내에서 시료의 항염증 효과를 평가하기 위해 MTT assay를 이용한 샘플의 독성평가와 nitric oxide 생성 저해 활성 및 염증관련 단백질 및 유전자의 발현량을 확인하였다. 시료의 미백 효과를 평가하기 위해서 멜라노마(B16F10 cell)를 이용해 MTT assay를 통한 샘플의 독성평가, cellular tyrosinase 저해율 측정 및 멜라닌 생합성량을 측정하였다. LPS로 유도된 RAW 264.7 세포내에서 시료의 nitric oxide 저해 활성 결과는 $25{\mu}g/mL$ 농도에서 71.2%의 우수한 효능을 나타내었으며 western blot 실험결과 iNOS, COX-2 단백질의 발현은 농도 의존적으로 감소하는 것을 확인하였다. Tyrosinase 활성 억제 실험에서 인삼복합물의 농도 $50{\mu}g/mL$에서 36.8%의 감소를 나타냈으며 멜라닌 생합성 억제 평가 결과에서 농도 $50{\mu}g/mL$에서 47.8%의 감소를 나타냈다. 상기 실험 결과로부터 인삼복합물의 우수한 항염증, 미백 효능을 확인하였으며 향후 안전한 천연 화장품 원료로 사용될 수 있음을 확인하였다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Signal transduction in Toxicology
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• pp.139-139
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• 2001

Arctigenin and demethyltraxillagenin, dibenzylbutyrolactone lignans, are phenylpropanoid plant metabolites with antioxidative and anti-inflammatory activities. The effects of arctigenin and demethyltraxillagenin on the nuclear factor-kB (NF-kB)-mediated inducible nitric oxide synthase (iNOS) gene expression were studied in Raw264.7 cells.(omitted)

• Biomolecules & Therapeutics
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• 제9권3호
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• pp.188-193
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• 2001

Free radical scavengers or quenching agents for reactive oxygen species (ROS) present in consumable fruits, vegetables, and beverages have received considerable attention as potential antioxidants, and thus uses for treatment of several human diseases. In this study, grapevine shoot extract (GSE) containing high concentration of resveratrol and viniferine was evaluated for antioxidant potential and inhibition of pro-inflam-matory mediator production. Utilizing 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and xanthine oxidase (XOD) inhibition assay the GSE showed inhibitory effects of DPPH radical scavenging and XOD activity with the $IC_{50}$/ values of 34.5 and 155 $\mu\textrm{g}$/ml, respectively. In addition, GSE also exhibited the inhibition of prostaglandin E$_2$ (PGE$_2$) and nitric oxide (NO) production in lipopolysaccharide (LPS)-induced mouse macrophage RAW264.7 cells with the $IC_{50}$/ value of 6.4 and 14.5 $\mu\textrm{g}$/ml, respectively. This result suggests that grapevine shoot extract has the potential activity as a natural antioxidant or antiinflammatory agent.

• 약학회지
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• 제54권5호
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• pp.387-391
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• 2010

Polygonum cuspidatum which is widely distributed in Korea has been used as treatments of dermatitis, gonorrhea and inflammation in traditional medicine. We examined anti-inflammatory activities by the inhibition of NO production in RAW264.7 murine macrophages cells. Phytochemical examination of Polygonum cuspidatum led to the isolation and characterization of emodin (1), emodin 8-O-${\beta}$-D-glucopyranoside (2), emodin 1-O-${\beta}$-D-glucopyranoside (3). Antioxidative activities of these compounds were determined by measuring the radical scavenging effects on DPPH radicals. Compounds 1 and 3 showed potent activities compared with L-NMMA. These results suggested that the antraquinone compounds isolated from Polygonum cuspidatum might be used as antiinflammatory agents.

• 약학회지
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• 제58권1호
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• pp.12-15
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• 2014

Polygonum cuspidatum which is widely distributed in Korea has been used as treatments of dermatitis, gonorrhea, favus athlete's foot, hyperlipidemia and inflammation in traditional medicine. We examined anti-oxidant and antiinflammatory activity by measuring DPPH radical scavenging activity and the inhibition of IFN-${\gamma}$ and LPS-induced NO production in RAW 264.7 murine macrophage cells. We isolated and characterized resveratrol (1), trans-resveratrol 3-O-${\beta}$-Dglucopyranoside (2). Compounds 1 and 2 showed potent activities compared with L-NMMA ($N^G$-monomethyl-L-arginine). These results suggested that the stilbene compounds isolated from Polygonum cuspidatum might be used as antiinflammatory agents.

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of International Convention of the Pharmaceutical Society of Korea
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• pp.145.1-145.1
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• 2001

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 NEW STRATEGY FOR DRUG DEVELOPMENT IN POST-GENOMIC ERA(대한약학회)
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• pp.215.2-215.2
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• 2000

• 한국자원식물학회지
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• 제32권4호
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• pp.264-269
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• 2019

쉽싸리(L. lucidus)가 가지는 효능의 하나로 알려진 항염증효과의 활성물질을 파악하기 위하여 본 연구를 수행하였다. 항염효과는 LPS로 활성화한 macrophage 264.7이 생산하는 NO의 감소효과를 측정함으로써 평가하였다. 쉽싸리 추출물에서 얻은 비극성 분획물인 $CHCl_3$ 분획물은 농도의존적으로 현저히 NO 생산을 감소시켰다. 이에 비해 극성 분획물인 BuOH 분획물은 그 효과가 약하였다. Silica gel column chromatography에 의해 이 $CHCl_3$ 분획물로부터 주요 화합물인 ursolic acid를 분리하고 분광학적 방법으로 동정할 수 있었다. 효과가 약하였던 BuOH 분획물로부터 diaion HP-20 column chromatography와 sephadex LH-20 column chromatography로 이 분획의 주요 화합물인 rosmarinic acid를 분리하고 역시 분광학적 방법으로 동정하였다. Ursolic acid는 농도의존적으로 NO 생산을 억제하였으나 rosmarinic acid는 그 효과가 상대적으로 약하였다. 이러한 사실로부터 쉽싸리의 항염효과는 주로 ursolic acid의 존재 때문임을 알 수 있었다.