• Asian-Australasian Journal of Animal Sciences
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• 제30권8호
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• pp.1183-1189
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• 2017

Objective: Owing to the public availability of complete genome sequences, including avian species, massive bioinformatics analyses may be conducted for computational gene prediction and the identification of gene regulatory networks through various informatics tools. However, to evaluate the biofunctional activity of a predicted target gene, in vivo and in vitro functional genomic analyses should be a prerequisite. Methods: Due to a lack of quail genomic sequence information, we first identified the partial genomic structure and sequences of the quail SH3 domain containing ring finger 2 (SH3RF2) gene. Subsequently, SH3RF2 was knocked out using clustered regularly interspaced short palindromic repeat/Cas9 technology and single cell-derived SH3RF2 mutant sublines were established to study the biofunctional activity of SH3RF2 in quail myoblast (QM7) cells during muscle differentiation. Results: Through a T7 endonuclease I assay and genotyping analysis, we established an SH3RF2 knockout (KO) QM7#4 subline with 61 and 155 nucleotide deletion mutations in SH3RF2. After the induction of myotube differentiation, the expression profiles were analyzed and compared between regular QM7 and SH3RF2 KO QM7#4 cells by global RNA sequencing and bioinformatics analysis. Conclusion: We did not detect any statistically significant role of SH3RF2 during myotube differentiation in QM7 myoblast cells. However, additional experiments are necessary to examine the biofunctional activity of SH3RF2 in cell proliferation and muscle growth.

• 대한간호학회지
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• 제43권4호
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• pp.526-535
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• 2013

Purpose: This study was done to compare clinical nurses' knowledge and visual differentiation diagnostic ability for the pressure ulcer classification system (PUCS) and incontinence-associated dermatitis (IAD). Methods: A convenience sample of 602 nurses took the pressure ulcer classification system and incontinence-associated dermatitis knowledge test (PUCS & IAD KT) and completed the visual differentiation tool (VDT), consisting of 21 photographs with clinical information. Results: The overall mean score for correct answers was 14.5 (${\pm}3.2$) in PUCS & IAD KT and 11.15 (${\pm}4.9$) in PUCS & IAD VDT. Incorrect responses were most common for statements related to stage III, IAD for PUCS & IAD KT, and suspected deep tissue injury (SDTI), unstageable, and stage III for PUCS & IAD VDT. Significant correlations were found between PUCS & IAD KT and VDT (r=.48, p<.001). Factors affecting scores for PUCS & IAD VDT were PUCS & IAD KT, frequency of pressure ulcer, IAD management and participation in wound care education programs. Conclusion: Results indicate that nurses have an overall understanding of PUCS & IAD, but low visual differentiation ability regarding stage III, SDTI, and unstageable ulcers. Continuing education is needed to further improve knowledge and visual differentiation ability for PUCS & IAD.

• Current Optics and Photonics
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• 제1권4호
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• pp.412-420
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• 2017

Multinucleated bone resorptive osteoclasts differentiate from bone marrow-derived monocyte/macrophage precursor cells. During osteoclast differentiation, mononuclear pre-osteoclasts change their morphology and biochemical characteristics. In this study, Raman spectroscopy with multivariate techniques such as Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were used to extract biochemical information related to various cellular events during osteoclastogenesis. This technique allowed for label-free and noninvasive monitoring of differentiating cells, and clearly discriminated four different time points during osteoclast differentiation. The Raman band intensity showed significant time-dependent changes that increased up to day 4. The results of Raman spectroscopy agreed with results from atomic force microscopy (AFM) and tartrate-resistant acid phosphatase (TRAP) staining, a conventional biological assay. Under AFM, normal spindle-like mononuclear pre-osteoclasts became round and smaller at day 2 after treatment with a receptor activator of nuclear $factor-{\kappa}B$ ligand and they formed multinucleated giant cells at day 4. Thus, Raman spectroscopy, in combination with PCA-LDA, may be useful for noninvasive label-free quality assessment of cell status during osteoclast differentiation, enabling more efficient optimization of the bioprocesses.

• 대한한방부인과학회지
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• 제30권4호
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• pp.175-202
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• 2017

Objectives: This study was conducted towards developing a screening tool for syndrome differentiation in the diagnosis of menopause in menopausal and perimenopausal women. Methods: We conducted a literature review of studies on menopausal diagnosis based on syndrome differentiation, and examined well-founded differentiated syndromes and their respective clinical symptoms. Based on the findings, we created a questionnaire through consultations with Oriental medicine experts in physiology, pathology, and diagnostics. Finally, the research team conducted an expert Delphi study on differentiated syndromes and the associated clinical symptoms. Results: Seven differentiated syndromes were selected, including Liver Depression (肝鬱), Kidney Yin Deficiency (腎陰虛), Kidney Yang Deficiency (腎陽虛), Liver and Kidney Yin Deficiency (肝腎陰虛), Kidney Yin and Yang Deficiency (腎陰陽兩虛), Heart-Kidney Noninteraction (心腎不交), and Dual Deficiency of Heart and Spleen (心脾兩虛); 4 disease locations, including liver (肝), heart (心), spleen (脾), and kidney (腎); and 3 disease natures, including Yin Deficiency (陰虛), Qi Stagnation (氣滯), and Blood Deficiency (血虛). In addition, we added 3 supplemental disease natures, including Yang Deficiency (陽虛), Qi Deficiency (氣虛), and Heat (火熱), in consideration of syndrome differentiation categories that may possibly be added in a follow-up clinical questionnaire. Conclusions: This resulted in a total of 7 differentiated syndromes, 4 disease locations, and 6 disease natures. We translated the clinical symptoms of these 17 categories into Korean Hangeul. After consulting with 5 Oriental medicine experts and a psychology expert, we produced a questionnaire for use in diagnosing menopause based on syndrome differentiation. The calculation of scores for the syndrome differentiation screening tool will be confirmed through clinical research based on the results of a review of existing literature.

• 경영과학
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• 제17권3호
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• pp.31-47
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• 2000

The widespread diffusion of Internet has enables companies to conduct business in quite a different way. marketing on the Internet becomes more important as firms adopt electronic commerce as a means to implement competitive strategies. One hundred and four BC shopping mall companies responded to an e-mail and web server survey about the anticipated benefits of electronic commerce and related strategies. The survey also asked about improvements in value chain activities and impediments to the implementation of electronic commerce in Korea. The results indicated that Korean shopping mall companies followed differentiation strategy significantly more than the other two strategies. In addition, six benefits factors were drawn from this study - information, productivity, cost savings, customers, management information, and applications. Productivity factor was related to cost leadership strategy, and customers factor was related with cost leadership, differentiation and focus strategies. Several benefits factors were also correlated with value chain activity improvements. The results of this study were compared with those of research conducted in the United States.

• Journal of Mechanical Science and Technology
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• 제19권spc1호
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• pp.350-356
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• 2005

A method of dynamic analysis of mechanical systems considering probabilistic properties is proposed in this paper. Probabilistic properties that result from manufacturing tolerances can be represented by means and standard deviations (or variances). The probabilistic characteristics of dynamic responses of constrained multi-body systems are obtained by two ways : the proposed analytical approach and the Monte Carlo simulation. The formerpaper, necessitates sensitivity information to calculate the standard deviations. In this a direct differentiation method is employed to find the sensitivities of constrained multi-body systems. To verify the accuracy of the proposed method, numerical examples are solved and the results obtained by using the proposed method are compared to those obtained by Monte Carlo simulation.

• 대한가정학회지
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• 제37권11호
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• pp.19-32
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• 1999

This study analyzed the curricula of fashion related majors at 4-year colleges in the USA, UK, Japan and Hong Kong as well as in Korea and compared the characteristics of the Korean curriculum with those of the four countries. A total of 124 curricula from 103 colleges were collected. Courses were divided into eight categories (including industry and market information; materials; merchandise planning; design; production; distribution and selling; basics; and consumption) representing the production and distribution process of fashion business. There were differences in course offerings among the five countries. Overall, curricula in Korea emphasized design and production (construction) areas and showed not much differentiation among colleges. While industry and market information, merchandise planning, and production (management) areas were emphasized in the USA , differentiation/specialization by major was observed . UK and Hong Kong had well-specialized curricula by major Japan seemed to offer traditional and consumption oriented courses. Based on the findings, some propositions for the Korean fashion curriculum were discussed.

• 한국통신학회논문지
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• 제30권2B호
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• pp.11-20
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• 2005

Strict priority scheduling scheme is a good candidate for the implementation of service differentiation in an Internet because of simplicity in implementation and the capability to guarantee the delay requirement of the highest class of traffic. However, it is also blown that strict priority starves the lower-class traffic at the cost of prioritizing the higher-class traffic. The purpose of this work is to propose an analytic method which can estimate the average delay performance of Diffserv service architecture and shows that strict priority scheme does not sacrifice the lower class traffic over a diverse condition of the load. From the numerical experiments for three-class Diffserv network we validate our argument that strict priority scheme may be applied to a service differentiation scheme for the future Internet.

• Journal of information and communication convergence engineering
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• 제4권4호
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• pp.141-146
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• 2006

In these days, wireless data services are becoming ubiquitous in our daily life because they offers several fundamental benefits including user mobility, rapid installation, flexibility, and scalability. Moreover, the requests for various multimedia services and the Quality of Service (QoS) support have been one of key issues in wireless data communications. Therefore the research relative to Medium Access Control (MAC) has been progressing rapidly. Especially a number of QoS-aware MAC schemes have been introduced to extend the legacy IEEE 802.11 MAC protocol which has not guaranteed any service differentiation. However, none of those schemes fulfill both QoS features and channel efficiency although these support the service differentiation based on priority. Therefore this paper studies a novel MAC scheme, referred to as Enhanced Distributed Coordination Function with Network Adaptation (EDCF-NA), for enhancements of both QoS and medium efficiency. It uses a smart factor denoted by ACK rate and Network Load Threshold (TH). In this paper, we study how the value of TH has effect on MAC performance and how the use of optimal TH pair improves the overall MAC performance in terms of the QoS, channel utilization, collision rate, and fairness. In addition, we evaluate and compare both the performance of EDCF-NA depending on several pairs of TH and the achievement of various MAC protocols through simulations by using Network Simulator-2 (NS-2).

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제5권4호
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• pp.720-740
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• 2011

Wi-Fi-enabled portable devices have recently been introduced into the consumer electronics market. These devices download or upload content, from or to a host machine, such as a personal computer, a laptop, a home gateway, or a media server. This paper investigates the fairness among multiple Wi-Fi-enabled portable devices in a home network when they are simultaneously communicated with the host machine. First, we present that, a simple IEEE 802.11-based home network suffers from unfairness, and the fairness is exaggerated by the wireless link errors. This unfairness is due to the asymmetric response of the TCP to data-packet loss and to acknowledgment-packet loss, and the wireless link errors that occur in the proximity of any node; the errors affect other wireless devices through the interaction at the interface queue of the home gateway. We propose a QoS-provisioning framework in order to achieve per-device fairness and service differentiation. For this purpose, we introduce the medium access price, which denotes an aggregate value of network-wide traffic load, per-device link usage, and per-device link error rate. We implemented the proposed framework in the ns-2 simulator, and carried out a simulation study to evaluate its performance with respect to fairness, service differentiation, loss and delay. The simulation results indicate that the proposed method enforces the per-device fairness, regardless of the number of devices present and regardless of the level of wireless link errors; furthermore it achieves high link utilization with only a small amount of frame losses.