• KSBB Journal
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• v.17 no.6
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• pp.577-581
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• 2002

In an attempt to produce recombinant proteins using the pollen enriched in some plant species, a 1.7 kb DNA encoding urease subunit B (UreB) amplified by PCR from Helicobacter pylori urease gene cluster in pH808 plasmid was cloned to be expressed under CaMV35S promoter in lily (Lilium longiflorum) pollen tubes elongated in vitro. Lily pollen at early germinating stage was transformed with the ureB DNA using Agrobacterium via vacuum infiltration and, incubated for a full pollen tube growth 16 - 24 h in the dark in the presence of kanamycin. DNA integration and expression in the transgenic pollen were analyzed by the standard molecular techniques and the results suggest that the pollen in vitro may be employed as a protein factory in a disposable fashion.

• Nuclear Engineering and Technology
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• v.32 no.3
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• pp.274-282
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• 2000

The simulation of water balance was conducted for suggested four alternative multi-layer cover design of near-surface radioactive waste disposal facility under domestic climate condition. The analysis was also conducted for the most favorable one out of four alternative cover design under conservative scenarios. Until 100 years after closure of disposal vault, the infiltration flux for the most favorable cover design was negligible even under doubling of the ambient precipitation condition. When the degradation of asphalt and geomembrane after 100 years of closure was considered, the infiltration flux significantly increased almost to the design criteria of cover system in I' Aube disposal facility. And it was found that the hydraulic conductivity of bentonite/sand as a bottom barrier should be no greater than 1$\times$10$^{-7}$ cm/sec recommended by U.S. EPA.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.3
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• pp.9-17
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• 2009

Most of the current drainage criteria have been developed on the basis of experimental field results and theoretical analyses of infiltration under saturated conditions. The objective of this study is to extend the understanding of pavement drainage systems by considering unsaturated condition in the sublayers. Analyses of unsaturated flows through pavements was performed by running finite element program(SEEP/W) with a range of pavement materials and drainage parameters. Meanwhile, the widely used DRIP program developed by FHWA is based on assumption of saturated condition of pavements. Differences between saturated and unsaturated condition in the sublayers of the pavements are verified. It is verified that for unsaturated conditions time to drain would take longer time compared to saturated condition.

• KSBB Journal
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• v.23 no.6
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• pp.554-556
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• 2008

Fresh Oenanthe javanica DC. leaves still attached to stem architecture were immersed in NaOH solution for 3 min before agroinfiltration and co-cultivation. MTT assay revealed that NaOH solution containing up to 0.7% was still safe for the leaf viability. Fluorometric GUS enzyme analysis showed that 0.5% NaOH-treated leaf tissues were efficiently transformed by vacuum infiltration for 20 min with Agrobacterium cells at a density of $OD_{600}=0.5$ to 1.0. These conditions worked well for the expression of HBsAg, which was confirmed by western blotting and ELISA.

• Journal of Korean Society of Water and Wastewater
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• v.25 no.1
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• pp.75-84
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• 2011

This study was conducted to provide a basic information for the establishment of operation and treatment processes in sewer system of Nakdong river basin to minimize the overall pollutants loading to water body. Sewage flowrates were regularly measured and monitored at various sampling points of newly-built separated sewer system located in G City GA sites. To assess the inflow sewage flowrate, various calculating methods such as water-use evaluation, average-minimum daily flow quality evaluation, minimum daily flow evaluation, night water-use evaluation were used. Average I/Is were calculated except water-use evaluation. Average I/Is were found to be 6.5 $m^{3}/d$, 3.5 $m^{3}/d$, 7.7 $m^{3}/d$ at GA-1, GA-2, GA-3 points respectively. I/I ratios of three areas were found to be 4.8 %, 2.0 % and 2.7 % respectively and were obviously lower than those of the other separated sewer systems as shown in the previous studies.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.181-181
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• 2017

Low impact development (LID) practices has become important to mitigate the damage from natural disasters in urban areas. Thereby many hydrological simulation models can simulate the hydrological impact of LID practices. However, commonly used models are not able to provide specific information to most users such as where LIDs should be placed and what kind of LID should be designed. In this study, a decision support system which can be used with the EPA's SWMM was developed for the determination of LID types and locations of LID practices, named Water Management Prioritization Module (WMPM), was applied to a urbanized university campus. Eight sub-catchments were selected as feasible candidate areas for the planning of LID practices. Pre-designated infiltration trenches and permeable pavements were applied to each selected sub-catchments, followed by peak and total runoffs comparison between before/after planning of LIDs. Moreover, TOPSIS, one of a multi-criteria decision analysis method was used in the procedure of selecting target sub-catchment areas and final prioritization of LID types and locations. As a result, sub-catchments S4 with permeable pavements and S16 with infiltration trenches has shown the most decrease in total and peak runoffs, respectively. Therefore, WMPM was found to be effective in determining the best alternative among various scenarios generated and simulated.

• Biomedical Science Letters
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• v.25 no.3
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• pp.282-287
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• 2019

Skin flap necrosis remains a major complication of reconstructive surgery. Euterpe oleracea Mart., popularly known as "acai berry" contains hydroxybenzoic acid, antioxidant polyphenolics and anthocyanins. These and other compounds within the acai berry confer anti-inflammatory and anti-oxidative effects. In this current study, we evaluated the protective effect of acai berry extracts on survival of random-pattern skin flaps in a murine model by histologic analysis. ICR mice were subjected to skin elevation surgery and orally administered acai berry extract (100 mg/kg) daily for 7 days. Tissues were stained with hematoxylin-eosin or Masson's trichrome to observe tissue integrity and collagen deposition. In addition, $TGF-{\beta}$ and VEGF was stained by immunofluorescence to determine anti-inflammatory cell infiltration and neovascularization, respectively. We found a decrease in inflammatory cell infiltration and increase in collagen deposition in the acai berry extract treated mice compared to control mice. Immunofluorescence staining reveal a higher number of $TGF-{\beta}$ positive cells and enhanced VEGF staining in the acai berry extract treated mice. The results from this study indicate that oral uptake of acai berry extract can promote healing and survival of surgical skin flaps in mice providing an augmentative therapeutic approach to enhancing skin flap survival.

• The Journal of Engineering Geology
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• v.22 no.3
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• pp.343-351
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• 2012

This study proposes a modified equation to calculate the factor of safety for an infinite slope considering the saturation depth ratio as a new variable calculated from rainfall infiltration into unsaturated soil. For the proposed equation, this study introduces the concepts of the saturation depth ratio and subsurface flow depth. Analysis of the factor of safety for an infinite slope is conducted by the sequential calculation of the effective upslope contributing area, subsurface flow depth, and the saturation depth ratio based on quasi-dynamic wetness index theory. The calculation process makes it possible to understand changes in the factor of safety and the infiltration behavior of individual rainfall events. This study analyzes stability changes in an infinite slope, considering the saturation depth ratio of soil, based on the proposed equation and the results of soil column tests performed by Park et al. (2011 a). The analysis results show that changes in the factor of safety are dependent on the saturation depth ratio, which reflects the rainfall infiltration into unsaturated weathered gneiss soil. Under continuous rainfall with intensities of 20 and 50 mm/h, the time taken for the factor of safety to decrease to less than 1.3 was 2.86-5.38 hours and 1.34-2.92 hours, respectively; in the case of repeated rainfall events, the time taken was between 3.27 and 5.61 hours. The results demonstrate that it is possible to understand changes in the factor of safety for an infinite slope dependent on the saturation depth ratio.

• Journal of the Korean Geotechnical Society
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• v.30 no.1
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• pp.37-47
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• 2014

Unsaturated hydraulic conductivity (HC) is integrated theoretically from soil water retention curves (SWRC) by Mualem capillary model, but the prediction of HC is extremely sensitive to small variation of matric suction near saturation. Near saturation, the Mualem HC based on smooth SWRC decreases abruptly and has problems in the reliability of hydraulic behavior and the stability of numerical solutions. To improve van Genuchten-Mualem (VGM) HC, the van Genuchten SWRC model is modified within range of low matric suction (arbitrary air entry pressure). At an arbitrary air entry pressure, the VG SWRC is linearized in log scale until full saturation. The modified VG SWRC does not affect the fit of actual retention behavior and either the parameters of original VG SWRC fit. Using the modified VG SWRC, the VGM HC is modified to integrate for each interval decomposed by arbitrary air entry pressure. An analytical solution on modified VGM HC is proposed each interval, to protect the rapid change in HC near saturation. For silty soils, VGM models of HC function underestimate the unsaturated permeability characteristics and especially show rapid reduction near saturation. The modified VGM model predicts more accurate HC functions for Korean weathered soils. Furthermore, near saturation, the saturated HC is conserved by the modified VGM model. After 2-D infiltration analysis of an actual slope, the hydraulic behaviors are compared for VGM and the modified models. The prediction by the proposed model conserved the convergence of solutions on various rainfall conditions. However, the solution by VGM model did not converge since the conductivity near saturation reduced abruptly for heavy rainfall condition. Using VGM model, the factor of safety is overestimated in both initial and final stage during heavy rainfall. Stability analysis based on infiltration analysis could simulate the actual slope failure by the proposed model on HC.

• The Journal of Engineering Geology
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• v.32 no.3
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• pp.327-338
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• 2022

This study aims to empirically and statistically predict soil depths across areas affected by landslides. Using soil depth measurements from a landslide area in Korea, two sets of soil depths are calculated using a Z-model based on terrain elevation and a probabilistic statistical model. Both sets of calculation results are applied to derive landslide risk using the saturated infiltration depth ratio of the soil layer. This facilitates analysis of the infiltration of rainfall into soil layers for a rainfall event. In comparison with the probabilistic statistical model, the Z-model yields soil depths that are closer to measured values in the study area. Landslide risk assessment in the study area based on soil depth predictions from the two models shows that the percentage of first-grade landslide risk assessed using soil depths from the probabilistic statistical model is 2.5 times that calculated using soil depths from the Z-model. This shows that soil depths directly affect landslide risk assessment; therefore, the acquisition and application of local soil depth data are crucial to landslide risk analysis.