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Expression of recombinant protein from Oenanthe javanica DC. leaf tissues as a biofactory  

Shin, Dong-Il (Department of Biotechnology, Catholic University of Daegu)
Park, Hee-Sung (Department of Biotechnology, Catholic University of Daegu)
Publication Information
KSBB Journal / v.23, no.6, 2008 , pp. 554-556 More about this Journal
Abstract
Fresh Oenanthe javanica DC. leaves still attached to stem architecture were immersed in NaOH solution for 3 min before agroinfiltration and co-cultivation. MTT assay revealed that NaOH solution containing up to 0.7% was still safe for the leaf viability. Fluorometric GUS enzyme analysis showed that 0.5% NaOH-treated leaf tissues were efficiently transformed by vacuum infiltration for 20 min with Agrobacterium cells at a density of $OD_{600}=0.5$ to 1.0. These conditions worked well for the expression of HBsAg, which was confirmed by western blotting and ELISA.
Keywords
Oenanthe javanica DC; leafy tissue; MTT; biofactory; HBsAg;
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