• Applied Biological Chemistry
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• v.40 no.2
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• pp.95-100
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• 1997

This study was aimed to survey inexpensive and reliable sources of chitinase from the animal origin. The stomach and its content of the broiler, the cod, the yellowtail and ${\beta}-glucuronidase$ from snail gut showed a considerable chitinolytic activity, while those of the bas didn't have any detectable activity. These crude enzymes was found to have both endo- and exochitinase activity. The effects of pH and temperature on the enzyme activity were variable. The hydrolytic products of colloidal chitin by the enzyme preparation from the broiler and the cod were chitooligomers having the degree of polymerization between 3 and 5. Furthermore we observed the chitosanolytic activity from these enzymes. In the degradation of chitosan the chyme of the broiler had the highest activity and ${\beta}-glucuronidase$ from snail gut followed. On the basis of the fact that the by-product of the broiler was not only commercially available but also the most potent in the endochitinase activity and the lowest in the exochitinase activity, we conclude that the gizzard and its chyme are considered as the most suitable source of the industrial chitinase among animals studied in this paper.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.4 no.1
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• pp.71-76
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• 2001

Purpose: Many diagnostic modalities for neonatal cholestasis have shown features that are helpful, however until recently none of them are not pathognomonic of biliary atresia (BA). We carried out a prospective study of infants with cholestatic jaundice with the aim of establishing an efficient method of diagnosing BA. Methods: Twenty-seven consecutive infants with cholestatic jaundice were enrolled in this study and ranged from 7 to 152 days in age (mean; $51.2{\pm}34.2$ days). Gastroduodenoscopy was carried out using a fiberscope (Olympus N30). All the babies were fasted for at least 4 hours before the procedure and 20 ml of 10% dextrose solution was given at the time of endoscopy. The endoscopic examination focused on the 5 minutes observation of the evidence of biliary secretion. If there was lack of the evidence of the biliary secretion, endoscopy was removed and repeated the examination with some pause. Results: There are lack of the evidence of biliary secretion in all infants with BA. In non-BA group, 8 out of the 10 infants showed biliary secretion on the first trial, however one (Alagille syndrome) of the two infants without evidence of biliary secretion, finally exhibited biliary secretion on the second trial. The above observations resulted in the diagnostic accuracy of 96.3% with 100.0% sensitivity and 90.0% specificity. Conclusion: In light of the results from our relatively small study, endoscopy is a convenient, and relative inexpensive procedure. we strongly support the use of endoscopy for the diagnosis of BA in the screening and evaluation of infantile cholestasis.

• Journal of Korean Society of Rural Planning
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• v.26 no.1
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• pp.77-88
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• 2020

This study had comparatively analyzed the influence factors of the farm village business on the local farmhouses centered on the level of importance and the level of performance. For this, a questionnaire survey had been conducted with 350 local residents who had participated in the Rural Field Forum of 14 villages, including Gyeongsangnam-do, Gyeongsangbuk-do, Chungcheongnam-do, Chungcheongbuk-do, Jeollabuk-do, etc. Excluding the insincere survey questionnaires, the final 232 people had been analyzed. As a result of the analysis of this study, the factors that have the influences on the diverse, rural areas had been compressed into the three factors of the lodging and the program, the special, rural area products, and the transportation and the accessibility. In the level of importance-level of performance analysis, it had been analyzed that the special, rural area products must maintain the continuity, that the transportation accessibility must avoid the excessive endeavor, and that the lodging program had a low priority order. Therefore, it can be said that it is necessary to concentrate the capability of the rural village business in the direction of developing the local specialties in the future. Next, after dividing the level of performance and the level of importance into the two groups each, the multiple group path analysis had been carried out. As a result, in the result of the analysis of the path that has an influence on the economic factor, the path coefficient of the level of importance of the transportation accessibility -> profit increase was 0.195+0.064 and the path coefficient of the level of performance of the transportation accessibility -> profit increase was 0.251+0.074. Therefore, both of them had the statistically significant amounts of the influences. This suggests that the transportation accessibility is an important element of the profit increase of the region. As a result, in the future, the research on the urban and rural exchanges using the even more convenient and inexpensive transportation means is needed. Most of them need the voluntary effort of the residents for being able to creatively develop and sell the native, agricultural, specialty products that are petty. Also, the rural areas need the diversities of the lodging programs and the recreational programs, a convenient reservation system, and the development of a unique tourism experience program. In the future, the researches that had been advanced in the even more detailed domain that is related to these factors are highly expected. Also, the analyses that had been carried out with even more areas and subjects as the targets are needed.

• Korean Journal of Clinical Laboratory Science
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• v.38 no.1
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• pp.16-21
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• 2006

Parallel testing means ordering a number of tests at the same time so abnormalities in any of the tests can be found quickly and used in making the diagnosis. This is a good medical strategy to eliminate diseases and it is relatively inexpensive if all the tests are potential sources of information and performed on the same analyzer. In regression, the equation for the straight line is recast as y = bx + a. This change in terminology leads to confusion. Here a is the y-intercept or constant and b is the coefficient or slope of the line. A few more words of caution about regression - as in all of statistics there are certain assumptions: the x value is a true measure, both X and Y distributions are normal, and homoscedasticity, i.e., the variance of y is the same for each value of x. In this study the linearity classification made by different scientists were always in agreement. Typical examples of curves that were considered linear are presented in Fig. 1-5. Because these automated procedures values were usually within five percent of each other the curvature could be easily detected. The plot of the WBC, RBC, hemoglobin, hematocrit and platelet concentrations from approximately 74.4 to $0{\times}10^3/{\mu}L$ and $80.4-0{\times}10^3/{\mu}L$, $5.6-0{\times}10^6/{\mu}L$ and $6.1-0{\times}1106/{\mu}L$, 18.3-0 g/dL and 19.0-0 g/dL, 54.1-0% and 56.8-0% and 642.0 to $0.03{\times}10^3{\mu}L$ and $754.0-0{\times}10^3/{\mu}L$ on the ADVIA 2120 C Versus and A and B typical of an acceptable linear study as shown in Fig. 1-5. The grand mean of R2, intercept and slope is 0.99898, 0.99459 and 1.54626.

• Korean Journal of Community Nutrition
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• v.23 no.2
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• pp.93-101
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• 2018

Objectives: With the increasing number of single households and so-called Honbab-jok, those who eat alone, people tend to enjoy convenient and simple meals, such as single dish meals. This study was performed to provide data on the energy and nutrient content, and nutritional adequacy of single-dish meal. Methods: From the literature reviews, 61 types of single-dish meals were selected, and divided into 4 groups (steamed rice, noodle, porridge, and sandwich burger), and a further 11 sub-groups (bibimbab, fried rice, topped rice, rolled rice/ warm noodle, cold noodle, seasoned noodle, dukgook/ porridge/, and sandwich, burger). In addition, 382 junior high school students from Gyeonggi, Sejong, Jeonbuk, and Chungnam areas were recruited for the survey. The survey questionnaires were composed of the characteristics, preference, and intake frequency of single-dish meals. The representative energy content (arithmetic mean) of single-dish meals were calculated, and compared with the energy contents of preference and intake frequency-weighted values adjusted. The representative nutrient contents, energy contribution ratio, and INQ (index of nutritional quality) of a single-dish meal were calculated for a nutritional adequacy evaluation. Results: The study subjects considered a single-dish meal as tasty, simple and fast to prepare, inexpensive, nutritious, and no low calorie food. The preference scores of all but 1 sub-group of singledish meals were ${\geq}5.00$ (5.00~5.97), and 1 sub-group (porridge) was 4.67 on a 7-point scale. The intake frequency of 11 sub-groups were 0.31~1.71/week, porridge was the lowest at 0.31 and warm noodles was the highest at 1.71. Fried rice, rolled rice, and warm noodle' intake frequency were ${\geq}1/week$. The representative energy of steamed rice, noodle, sandwich burger were 443, 429, and 428 kcal, respectively, and that of porridge was 264 kcal. Less than 5% differences in the representative energy of 4 groups were observed when adjusted for the preference or intake frequency-weighted values. The energy contribution ratio of macro-nutrients calculations showed that porridge was a high carbohydrate and low fat food, whereas sandwich burger were high fat and low carbohydrate foods. The INQ of calcium and vitamin C were less than 1.0 in all 4 food groups, but the INQ of protein and thiamin were > 1.0 in all 4 single-dish food groups. Conclusions: The representative energy in the 4 groups of single-dish meal was 264~450 kcal, which is a rather low calorie meal, and the energy contribution ratio of macro-nutrients were inadequate. The protein and thiamin levels were sufficient but the calcium and vitamin C levels were insufficient in all 4 groups of a single-dish meal judged by the INQ. The additional intake of fruits and milk dairy products between meals with a single-dish meal, supply of calcium and vitamin C may increase, which will result in an improved nutritional balance.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.2
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• pp.1-8
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• 2012

This study is carried out to find the most optimal soil-plant combination in the urban agriculture by analyzing the association of soil base material which is being used in the urban agriculture with the growth of plants. 4 types of easily purchased soil (bed soil(A), animal vermicast soil(B), earth worm soil(C) and matured compost(D)) verified in aspects of effect and safety of soil in terms of growth of crop is selected as experimental soil and B, C, D type soils are mixed with granite soil at the ratio of 7 : 3. And granite soil(E) is set as a controlled soil and is compared to verify the effect of the experimental mixed soil. Herbaceous plants are classified into the fruit vegetables (Lycopersicon esculentum Mill. and Capsicum annuum L.), leafy vegetables (Brassica campestris L. ssp. Pekinensis and Lactuca sativa L.), medicinal vegetables (Chrysanthemum zawadskii var. latilobum and Liriope platyphylla F. T. Wang & T. Tang). The results of comparison of growth of herbaceous plants in different soil types showed that fruit vegetables and leafy vegetables in general had excellent growth in D type soil mixture and A type soil in general and had the poorest growth in E type (controlled) soil. 'Chrysanthemum zawadskii var. latilobum' had the excellent growth in D type, B type, C type mixed soil and A type soil and E type (controlled) soil are followed in order. In the case of 'Liriope platyphylla F. T. Wang & T. Tang', the difference in growth by each soil was shown to be insignificant. Therefore, the soil applied in the urban agriculture varies depending on each species of herbaceous plants, but it is considered effective to cultivate herbaceous plant which is economical and productive by using D type mixed soil which can be recycled and inexpensive compared with other experimental soils in the urban agriculture.

• Journal of Embryo Transfer
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• v.25 no.4
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• pp.229-235
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• 2010

The MTT assay is one of superior evaluation methods widely used to analyze the viability of metabolically active cell. It can be used to determine the percentage of viable sperm through measurement of the reduction of MTT granules at mitochondria in sperm tail. The purpose of this study is to determine the optimal condition of a simple and easy MTT assay to validate boar sperm viability and compare the accuracy of this test with microscopic examination. The MTT reduction rate for sperm viability were analyzed in microtiter plates (96 well) from 1 hr to 5 hr incubation periods at $37^{\circ}C$ using spectrophotometer (microplate reader) at 550 nm wavelength. The remainder of semen sample was simultaneously examined to compare the correlation of accuracy between MTT assay and other sperm parameters. Those sperm parameters were included the motility, survival rates, membrane integrity, mitochondria activity and acrosome integrity. The OD values of MTT assay (MTT reduction rates) did not greatly change at 1 hr to 5 hr incubation periods in different proportion of live and freeze-killed sperms (dead sperm). The MTT reduction rates or survival rates were decreased according to the different concentration of live and dead sperm. The linear regression at 1 hr and 4 hr incubation periods in sperm MTT assay was y=291.55x-72.176 and y= 180.64x-44.569, respectively. There are high correlation between 1 hr and 4 hr incubation periods (p<0.001). The results of MTT assay and other sperm parameters has a positive correlation (p<0.01 or 0.05). The correlation coefficients for MTT assay was 0.88115 for motility, 0.89868 for survival rates, 0.91722 for membrane integrity and 0.77372 for acrosome integrity, respectively. In conclusion, the MTT assay can be used as a reliable and efficient evaluation method for boar sperm viability. It can be use practical means to evaluate the quality of boar sperm by a fast, inexpensive and easy method.

• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.531-537
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• 2000

The simple microgel electrophoresis of single cells, a 'comet assay', on fruit seeds enabled the rapid identification of irradiated fruits by comparing the intact non-irradiated cells and the damaged cells of irradiated fruits. Grapes and plums were irradiated with 0.1, 0.5, 0.7, 1.0 kGy and strawberries, peaches, apples, and nectarines were irradiated with only 1.0 kGy. Seeds were isolated, crushed, and the suspended cells were embedded in an agarose layer. After lysis of the cells, they were subjected to microgel electrophoresis for 2 minutes, and then stained. The DNA radiation-induced fragmentation of all the fruits stretched and migrated out of the cells forming a tail toward the anode giving the appearance of a comet, while the undamaged cells appeared as intact nuclei without tails. Grape and plum seeds irradiated at 0.5 kGy and higher showed significant increases in tail length. With increasing the irradiation doses, longer extention of the DNA from the nucleus toward the anode was observed. Strawberry, peach, apple, and nectarine seeds irradiated with 1.0 kGy also showed the longer tails than non-irradiated ones. DNA comet assay as a rapid and inexpensive screening technique could be an officially validated method for the detection of irradiated fruits.

• 한국지형공간정보학회:학술대회논문집
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• 2004.10a
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• pp.5-10
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• 2004

The purpose of this research is to measure a gravitational attraction about urban pulbic service facilities and to evaluate the location of public service facilities. It is important in that these facilities should provide more inexpensive and convenience public service to users. To do this, the GIS's spatial analysis and gravity model were used to analyze the efficiency of the public facilities. The gravity model was conducted as the main analysis method, and another model for this analysis was the discrete model. The gravity model is originally to anticipate migration flows, traffic flows and other types of movements so that this model compares the gravitational attraction between places. The discrete model is to find the optimal location and to evaluate a location of facility regarding urban areas as the combination of node and link. In this research, these two models were adopted to compare and analyze location of Mapo-Gu Office. The results of this research indicated that the locational evaluation of urban public service facilities discovered the appropriateness of those facilities, and the public facilities was necessary to displace to other site.

• Journal of Aquaculture
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• v.16 no.3
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• pp.171-178
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• 2003

Inorganic nutrients such as nitrogen and phosphate compounds accumulate in recirculating aquaculture systems. These nutrients must be removed from the system before they affect pH and fish health. For this purpose, aquatic plants are a simple and inexpensive method of removal. There are four commonly used aquatic plants: Eichhornia crassipes (water hyacinth), Pistia stratiotes (water lettuce), Hygrophila angustifolia, and Hydrocotyle leucocephala in freshwater recirculating aquaculture systems in Korea, but their efficiencies are not known. Therefore, removal efficiencies of inorganic nutrients from a freshwater recirculating aquaculture water with four commonly used aquatic plants were tested. Removing efficiencies of TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N of the plants in 210 L aquaria for 48-hour period were tested. The removing efficiencies of TAN, N $O_3$$^{[-10]}$ -N, and P $O_4$$^{3-}$-P of the two most effective plants, water hyacinth and water lettuce, were also tested in 690 L (surface area of 1.55 $m^2$) tanks under 2 different initial stocking densities, 4 kg and 6 kg, for 22 days. Proximate analysis major nutrients and N and P contents of the all plants were analyzed for calculating net removal weight of N and P by the plants. Water lettuce was the most effective for removing TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N from the water for 48-hour period tested followed by water hyacinth and Hygrophila angustifolia. Water lettuce reduced TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N concentration from 2.3 mg/L, 0.197 mg/L, and 21.4 mg/L to 0.4 mg/L, 0.024 mg/L and 17.4 mg/L, respectively while water hyacinth reduced them down to 0.6 mg/L, 0.029 mg/L and 17.9 mg/L, respectively. The concentrations of TAN, N $O_2$$^{[-10]}$ -N, and N $O_3$$^{[-10]}$ -N in Hydrocotyle leucocephala group were rather increased up to 3.7 mg/L, 5.7 mg/L and 48.2 mg/L, respectively. This is because the creeping stem of Hydrocotyle leucocephala had to be cut to meet stocking weight resulting in decaying of the stem in the aquaria during experiment. The net growth in weight of water hycinth and water lettuce of 4 kg each in the 1.55 $m^2$ tanks for 22 days were 9.768 kg and 10.803 kg respectively, and those at initial weight of 6 kg each were 8.393 kg and 9.433 kg, respectively. The reason of lower net growth in the later group was restricted growth space. Nitrogen and phosphorus contents in water hyacinth were 2.89% and 0.27%, and those in water lettuce were 3.87% and 0.36%, respectively. Average quantities of removed N and P from 1.55 $m^2$ tanks by water hyacinth for 22 days were 18.9 g and 1.75 g, while those by water lettuce were 36.8 g and 3.5 g, respectively. Therefore water lettuce showed much higher efficiencies for removing both N and P from recirculating aquaculture water than water hyacinth.