• Journal of the Korean Society of Industry Convergence
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• v.26 no.1
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• pp.47-57
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• 2023

In this study, the bioactive activities (antioxidant activity, whitening activity, and anti-wrinkle effect) of dried tapioca-unhulled barley grain residue (TUDDG) obtained after alcohol fermentation, were measured. In the case of DPPH radical scavenging activity, the 50% ethanol extract of TUDDG showed the highest level of about 83% at the concentration of 100 mg/ml, and the ABTS radical scavenging activity was also about 98% of the 50% ethanol extract of TUDDG even at the concentration of 10 mg/mL. In the case of mushroom tyrosinase inhibitory activity, the 50% ethanol extract of TUDDG showed the highest activity at 100 mg/ml concentration of 37%. As a result of collagenage activity inhibition and elastase analysis, the 50% ethanol extract of TUDDG showed high activity with 4.2 mg/mL (IC₅₀) and 26.1 mg/mL (IC₅₀), respectively. Therefore, considering the physiological activity as well as the extraction efficiency of physiologically active substances, the 50% ethanol extract of TUDDG is judged to be highly effective.

• Proceedings of the KSR Conference
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• 2003.10b
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• pp.8-13
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• 2003

For continuous improvement of the railway customer satisfaction service, more objective evaluation and development system for software and hardware shall be built. The entire activity of railway customer is the interface between the customer and software/hardware services. Customer's activity was investigated by participating observation and objective observation, and considering the connection of all the activities, it was divided into 9 categories and 43 items. Although the detailed activity of customer would vary per item, they show more than 430 different activities under general condition. Also, the customer's activity varies according to the numerous variants such as physical, mental, environmental and cultural features. Especially, as lots of changes, diversification and high-quality of railway industry are expected according to the middle and long term plan on railway industry development, it is necessary for experts in various fields to perform cooperative research aggressively to improve the customer satisfaction service continuously based on continuous customer activity research.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.29 no.1
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• pp.43-51
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• 1997

The various culture conditions of Trichoderma viride(ATCC 3454) and Phanerochaete chrysosporium(ATCC 26921) with glucose-pepton medium, Mandels medium, YMG medium for wood degradable enzyme were examined. Mycellium of the two species grew profusely on glucose-pepton medium. Maximum fungal growth was observed about 10days. But CMCase, Fpase, laccase activity in the culture medium with glucose-pepton was not detected. When grown in fermenter culture using Mandels medium, Trichoderma viride produced CMCase and Fpase. Its CMCase activity was 0.15 lU/ml and Fpase activity was 0.3 IU/ml within about 4-6days. Phanerochaete chrysosporium grown in a YMG medium gave the best enzyme activity when they were grown under stationary culture with an atmosphere of 100% oxygen. Levels of laccase activity of 3.0 mull were achieved in stationary culture under 100% oxygen. The enzyme condensation by ultrafiltration method caused a 2-fold(cellulase) and 6-fold(laccase) as compared to control activity.

• Journal of Korea Society of Digital Industry and Information Management
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• v.17 no.4
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• pp.121-132
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• 2021

This study analyzed the effect of the extracurricular activity management system on user satisfaction. For this purpose, the effects of content, navigation, screen frame, design, interaction, and error handling factors on user satisfaction were analyzed. A survey was conducted on 321 students of K University located in Chungcheongbuk-do, and the research results based on the survey contents are as follows. First, content, navigation, screen frame, interactivity, and error handling, which are major elements of the extracurricular activity management system, showed statistically significant results. Second, interactivity and error handling were found to have the greatest influence on the factors affecting user satisfaction of the extracurricular activity management system. In this study, it was found that the interaction of the whole system including contents is important for continuous improvement of the extracurricular activity management system, and that it has a positive effect on user satisfaction when prompt error handling is possible.

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1827-1834
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• 2015

The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the binding site groove, on the function of SMG1 lipase. Site-directed mutagenesis was conducted at these sites, and each of the mutants was expressed in the yeast Pichia pastoris, purified, and characterized for their activity toward DAG and p-nitrophenol (pNP) ester. Compared with wild-type SMG1, F278A retained approximately 78% of its activity toward DAG, but only 11% activity toward pNP octanoate (pNP-C8). L103G increased its activity on pNP-C8 by approximately 2-fold, whereas F104G showed an approximate 40% decrease in pNP-C8 activity, and they both showed decreased activity on the DAG emulsion. The deletion of 103-104 retained approximately 30% of its activity toward the DAG emulsion, with an almost complete loss of pNP-C8 activity. The deletion of 103-104 showed a weaker penetration ability to a soybean phosphocholine monolayer than wild-type SMG1. Based on the modulation of the specificity and activity observed, a pNP-C8 binding model for the ester (pNP-C8, N102, and F278 form a flexible bridge) and a specific lipid-anchoring mechanism for DAG (L103 and F104 serve as "anchors" to the lipid interface) were proposed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.3
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• pp.269-277
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• 2006

This study prepared functional oyster hydrolysates using two-step enzymatic hydrolysis and investigated their functional properties. To prepare two-step enzymatic hydrolysates (TSEH), oysters were hydrolyzed using 1% Protamex (PR) at $40^{\circ}C$ and pH 6.0 for 1 hr before sequential treatment with one of the following enzymes for 1 hr: Alcalase (AL), Flavourzyme (FL), Neutrase (NE), pepsin (PE), and trypsin (TR). The PRAL, PRNE and PRTR hydrolysates had significantly greater angiotensin I converting enzyme (ACE) inhibitory activity than did PR and the other TSEHs. Only the antioxidant activity of the PRNE hydrolysate was significantly different (p<0.05), while none of the TSEHs had antimicrobial activity. The oyster hydrolysate prepared by sequential treatment with Protamex and Neutrase (PRNE) had the best ACE inhibitory activity and antioxidant activity, with $IC_{50}$ values of 0.40 and 0.94 mg/mL, respectively. The PRNE hydrolysate was processed through an ultrafiltration (UF) series with molecular weight cut-off (MWCO) membranes of 3, 5, 10, and 30 kDa, and the ACE inhibitory, antioxidant, and antimicrobial activities of the permeates were determined. The permeate through the 3-kDa MWCO membrane had greater ACE inhibitory activity and antioxidant activity than did the other PRNE permeates, with $IC_{50}$ values of 0.11 and 0.40 mg/mL, respectively.

• Journal of Environmental Science International
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• v.24 no.7
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• pp.917-925
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• 2015

Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and leads to environmental problems. It destroys the beach scenery and causes a bad smell and pollution by trashing on the seashore. Therefore, purpose of this study was to investigate antioxidant and antihypertensive activities of different solvent extracts from S. clava tunic for recycling of fishery waste. Antioxidant and antihypertensive activities of all extracts were concentration-dependent. Of extracts, hot water extract showed the highest DPPH radical scavenging activity with the lowest effective concentration ($EC_{50}$) value (0.733 mg/ml). Chloroform extract exhibited the highest metal chelation activity with the lowest $EC_{50}$ value (2.696 mg/ml). Autoclaved water extract showed the highest NO radical scavenging activity with the lowest $EC_{50}$ value (0.491 mg/ml) and n-hexane extract showed the highest reducing power ($A_{700}=1.897$ at 100 mg/ml). And n-butanol extract showed the highest SOD-like activity with the lowest $EC_{50}$ value (19.116 mg/ml) and ACE inhibition activity with the lowest inhibitory concentration($IC_{50}$) value (0.149 mg/ml). These results indicate that extracts obtained from S. clava tunic may potential candidate to reduce diseases caused by various oxidative stresses and hypertension.

• Fisheries and Aquatic Sciences
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• v.14 no.3
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• pp.168-173
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• 2011

The purpose of this study was to obtain a fraction with high antioxidative activity from second rockfish gelatin hydrolysates (SRSGHs), which were hydrolyzed with Alcalase and Flavourzyme through ultrafiltration membranes with serial digestions for 1 and 2 h, respectively, and to investigate the feasibility of this fraction as a potential functional food ingredient. Among various fractions that were ultrafiltered from the SRSGH with four types of membrane (1, 5, 10, and 30 kDa), the SRSGH-III fraction, which permeated the 10 kDa membrane but not the 5 kDa membrane, showed the highest antioxidant activity (protection factor=5.13) and angiotensin-I-converting enzyme-inhibiting activity ($IC_{50}$=0.82 mg/mL). These results suggest that the SRSGH-III fraction from the SRSGH can be used as a functional food ingredient. However, further studies examining its antioxidant activity in vivo as well as the different antioxidant mechanisms are needed.

• Journal of the Korean Wood Science and Technology
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• v.50 no.6
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• pp.414-426
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• 2022

The Lauraceae family has commercial uses, such as in the food, pharmaceutical, and perfume industries. This study was conducted to investigate anti-asthmatic activity of essential oils from the seven species in the Lauraceae family. The essential oils were extracted from the leaves of seven species, and the chemical composition was investigated by gas chromatography-mass spectrometry. The major constituents of essential oils differed depending on the species, even if they belonged to the same family. The main constituents were camphor (89.09%) in Cinnamomum camphora, linalool (26.91%) in Cinnamomum cassia, 1,8-cineole (23.90%) in Cinnamomum japonicum, d-limonene (10.27%) and β-eudesmol (10.03%) in Lindera obtusiloba, δ-cadinene (13.85%) and α-phellandrene (11.57%) in Machilus japonica, cis-,trans-β-ocimene (13.80% and 12.06%) and elemol (11.46%) in Neolitsea aciculata, and cis-β-ocimene (37.94%) and sabinene (24.91%) in Neolitsea sericea. The anti-asthmatic activity of essential oils was investigated using the lipopolysaccharide-induced NCI-H292 cells. The relative expression levels of the pro-inflammatory cytokines [interleukin (IL)-1β and IL-6] and mucus gene (MUC5AC and MUC5B) were significantly reduced by essential oils from seven species in the Lauraceae family. Among the seven essential oils, the essential oil from L. obtusiloba had the most superior anti-asthmatic activity. These results suggest that the essential oil of L. obtusiloba leaves could be used as an agent to suppress mucus hypersecretion.

• Korean Journal of Construction Engineering and Management
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• v.13 no.4
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• pp.78-88
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• 2012

As worldwide efforts to reduce global warming gases, the construction Industry is endeavoring to diminish carbon dioxides emissions. Especially, by introducing the LCA methodology to the industry, A variety of related studies to measure the emission of carbon dioxides have been conducted. However, when the conventional LCA methodology is applied to the construction projects, some limitations have been reported. To overcome the restrictions derived from the industry characteristics, this research suggested the Activity-based LCA model by applying the Activity-based Costing (ABC), which breaks down the whole life cycles into more detailed stages. By implementing the newly developed model, forecasting accuracy of $CO_2$ emission was elevated, and the critical control points on carbon dioxides were established. Through the case study of aluminium curtain-wall system, this research verified the usefulness of the Activity-based LCA.