• International Journal of Industrial Entomology and Biomaterials
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• 제4권1호
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• pp.31-36
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• 2002

Peptidohlycan recognition protein (PGRP) is one of the pattern recognition proteins in innate immunity of insect. We isolated differentially expressed two cDNAa, BTL-LPI and BTL-LP2, in the fat body of Bombyx mori larvae injected with bacteria by subtractive hybridization method. These two clones showed amino acid sequence divergence of 30.4%. In the comparison with other insect PGRP genes, BTL-LP2 showed 48.8% and 45.2% of sequence homology to the known PGRP genes of Bombyx mori and Tricoplusia ni, respectively, and BTL-LP2 was 31.8% and 30.9% , respectively. Phylogenetic analysis showed relatively close relationship of the BTL-LP2 to the known insect PGRP, unlike BTL-LPI, which was equidistant both to insect and mammals, suggesting a divergent relationships of the two newly cloned B. mori PGRP genes. Northern blot analyses confirmed an induction of the expression of BTL-LP2 by the bacterial infection in the Int body of B. mori, suggesting the involvement of the gene in the insect immunity.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.193-197
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• 2014

To investigate the degree of individuals' likability of insects, a survey was conducted with students and adults living in Korea. Overall, for insect-related questions, positive responses were higher than negative ones. Among the positive responses, the personal rearing of insects scored lower than others. The unconditional dislike of insects scored highest in the negative responses. A significant differences and correlations were found between males and females, students and adults, people experienced in insect-related events and those who were not. In particular, previous experience in insect-related events significantly influenced insect likability. Subjects preferred coleopteran and lepidopteran insects to others. However, this preference of insects differed by gender: Male participants preferred coleopteran insects to lepidopteran insects, while female participants preferred lepidopteran insects to coleopteran ones. No significant differences in the preference of insects were found by participants' job, residence, or experience in insect-related events. These findings suggest that gender is the most important factor to consider in selecting insects to be used as therapeutic tools in mental health care.

• International Journal of Industrial Entomology and Biomaterials
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• 제31권1호
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• pp.7-13
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• 2015

In an effort to obtain foundational data required to evaluate the therapeutic effect of educational insect rearing, this study surveyed current educational pet insect rearing practices in Korea and examined the expected psychological effects from participating in the activity. Data were collected from those who visited the special insect exhibition in May 2013 using a self-reporting survey. The results indicated that 48.3% of the responders had experienced insect rearing, and that rhino and stag beetles were the most commonly reared insects. Most of the respondents (83.1%) reported they were not currently rearing any insects, which suggested that insect rearing tends to be a one-time experience. Expected psychological effects could be divided into emotional (56.3%) and cognitive (41.8%) effects, and these varied by age and gender. The differences in expected psychological effects were particularly clear between adults and adolescents, the latter of whom were the main participants in the activity. It is therefore necessary to develop standardized manuals and care programs for more diverse insect species with which to help keep up consumer interest in educational insect rearing. These efforts will help champion the psychotherapeutic effects of educational pet insect rearing and enhance its role as a learning aid.

• 농업과학연구
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• 제45권4호
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• pp.655-663
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• 2018

The insect gut microbiome is known to have important roles in host growth, development, digestion, and resistance against pathogens. In addition, the genetic diversity of the insect gut microbiota has recently been recognized as potential genetic resources for industrial bioprocessing. However, there is limited information regarding the insect gut microbiota to better help us understand their potential benefits for enhanced pig production. With the development of next-generation sequencing methods, whole genome sequence analysis has become possible beyond traditional culture-independent methods. This improvement makes it possible to identify and characterize bacteria that are not cultured and located in various environments including the gastrointestinal tract. Insect intestinal microorganisms are known to have an important role in host growth, digestion, and immunity. These gut microbiota have recently been recognized as potential genetic resources for livestock farming which is using the functions of living organisms to integrate them into animal science. The purpose of this literature review is to emphasize the necessity of research on insect gut microbiota and their applicability to pig production or bioindustry. In conclusion, bacterial metabolism of feed in the gut is often significant for the nutrition intake of animals, and the insect gut microbiome has potential to be used as feed additives for enhanced pig performance. The exploration of the structure and function of the insect gut microbiota needs further investigation for their potential use in the swine industry particularly for the improvement of growth performance and overall health status of pigs.

• 생명과학회지
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• 제31권12호
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• pp.1094-1099
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• 2021

동애등에(Hermetia illucens)에 대한 몇 가지 약리적 효능이 보고된 바 있으나, 이 추출물의 항비만 및 작용기전에 대한 보고는 전무한 실정이다. 본 연구에서는 3T3-L1 지방전구세포를 지방세포로 분화시키는 과정에 동애등에 유충 에탄올 추출물을 첨가하여 분화 억제를 통한 항비만 효과를 확인하고자 하였다. 지방세포 분화에서 나타나는 중성지방의 양을 Oil red O 염색을 통하여 지방 축적 정도를 측정하였고, 지방세포의 분화와 지방 합성에 관련된 인자들의 발현량을 western blot을 통해 확인하였다. 동애등에 추출물에 의해 지방세포의 지방구 형성은 유의한 수준으로 억제되었다. 또한, 지방세포의 분화에 관여하는 전사인자인 peroxisome proliferator-activated receptor γ (PPARγ)와 CCAAT enhancer-binding protein α (C/EBPα)의 단백질 발현은 400 ㎍/ml 동애등에 추출물에 의해 현저하게 감소되었다. 이에 본 연구결과를 토대로 동애등에 추출물은 지방세포 분화 및 지방 축적 억제를 통한 항비만 소재로 활용하기에 적합할 것으로 판단된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권1호
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• pp.57-62
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• 2002

Cationic liposomes complexed with DNA have been extensively utilized for the delivery of reporter or therapeutic genes both in culture and in vivo. We investigated and determined the optimum conditions of a cationic liposome, composed of dimethyldioctadecy-lammonium bromide (DDAB) and dioleoyl phosphati-dylethanolamine UOPE), mediated a reporter plasmid expressing luciferase into insect cell lines (Sf-21 and Bm-N) and silkworm larvae. Together the data demonstrated that Bombyx mori nuclear polyhedrosis virus (BmNPV) genomic DNA (128 kb) was successfully transfected into Bm-5 cells using this liposome. These results suggest that DDAB/DOPE liposome will be useful as delivery agents for gene transfer to insect cells both in vitro and in vivo.

• 한국응용곤충학회지
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• 제46권3호
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• pp.363-373
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• 2007

우리나라 도시 및 공단지역의 농경지에 대해 서식하고 있는 지표곤충 및 평가지수를 이용하여 농업생태계 내의 환경상태를 평가하고자 2004년부터 2006년까지 곤충 서식환경이 다른 지역을 대상으로 곤충 상을 조사하였다. 각 조사지역 내에서의 서식식물을 조사하여 식생을 평가한 결과 농업지역의 자연성이 좋았으며, 도시지역 과 공단지역은 자연환경 상태가 다소 떨어졌다. 각 지역별 수질, 토양 및 대기환경을 조사한 결과 농업지역에 비해 도시지역과 공단지역의 수질, 토양중금속, 대기 등 오염이 더 심한 것으로 나타났다. 5월부터 10월까지 서식곤충을 조사한 결과 조사지역 모두 6월부터 8월까지 가장 많은 곤충이 채집되었으며, 농업지역은 총 12목 106과 166종, 도시지역은 11목 102과 148종, 공단지역은 11목 100과 152종으로 농업지역에서 가장 많은 곤충이 서식하고 있었다. 목별로는 딱정벌레목의 곤충이 가장 많이 서식하였으며, 곤충 군집분석 결과 다양도 지수는 농업지역이 2.36으로 도시지역(1.92)과 공단지역(1.28) 보다 높았다. 이상의 결과를 종합하여 평가 항목별 기준을 보면 양호한 지역은 농업지역이며, 곤충 다양성 지수 2.1이상, 지표곤충은 남방폭탄먼지벌레, 환경조건은 수질 BOD 4.8 ml/L, 토양중금속 Cd 0.06 ppm이하였다. 보통인 지역은 도시지역이며, 곤충 다양성 지수 1.5-2.0, 지표곤충은 끝동매미충, 환경조건은 수질 BOD 10.98 ml/L, 토양중금속 Cd 0.30 ppm이하였다. 그리고 불량한 지역은 공단지역이며, 곤충 다양성 지수 1.5미만, 지표곤충은 콩잎벌레, 환경조건은 수질 BOD 29.7 ml/L, 토양중금속 Cd 1.01 ppm이었다. 따라서 도시 및 공단지역은 농업지역에 비해 곤충이 서식할 수 있는 환경조건, 특히 식생, 수질, 토양환경이 떨어졌으며, 서식하고 있는 곤충 종수, 개체수 등 서식밀도가 낮은 것으로 보아 이들 농경지에 대한 건전성을 평가할 수 있는 일부 기준이 될 것으로 사료된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.198-206
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• 2014

The olive fly Bactrocera oleae (Rossi) is the key pest for olive cultivation worldwide. Substantial effort has been invested in the development of the sterile insect technique (SIT) to control this pest. One of the limitations to develop SIT technology for olive fruit fly is the low ability of wild females to lay eggs in other medium than olive fruits, and their slow adaptation to oviposition in artificial substrates. In the present study, fruit grapes were used as an alternative egg collection medium to harvest eggs and young larvae from freshly colonized wild strains originating from France, Italy, Spain and Croatia. The larvae were allowed to develop into the fruits until the second instar, before they were extracted out and further reared on a standard artificial diet. Furthermore, F1 to F4 female flies were alternatively offered wax bottles to oviposit. Finally, the performance of hybrid strains created from crosses between wild and long colonised flies was assessed. The results showed that females of all 4 wild strains readily oviposited eggs in grapes and from the F2 generation onward, females from all strains were adapted to laying eggs in wax bottles. No difference was observed in eggs and pupae production among all strains tested. The findings are discussed for their implications on SIT application against olive fruit fly.

• 대한본초학회지
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• 제35권2호
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• pp.39-46
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• 2020

Objective: In this study, we analyzed the trend of patents registered prior to October 4, 2019, to understand the industrial trends in insect-derived medicinal materials used for the treatment of skin diseases. Methods: Using the WIPSON database, we collected information regarding the patents related to insect-derived materials for treating various skin diseases. Results: The patents registered prior to October 4, 2019, from Korea, Japan, the USA, China, and the EU, along with those registered under PCT were selected. There were 195 patents related to the use of insect-derived medicinal materials in treating various skin diseases such as psoriasis, inflammatory skin diseases, eczema, pruritus, and atopic dermatitis. China is mostly superior in total number of registered patents compared with the other countries. Korea was the major patent technology-holder for atopic dermatitis, but China dominated in the remaining categories of skin diseases. Upon first patent registration in 1992, there had been a continual increase in the number of patents. Especially, patents related to eczema, psoriasis, inflammatory skin disease were markedly increased. Most frequently used insect-derived medicinal materials was Scolopendra, Cicadidae Periostracum, Scorpions, Cantharides, and Batryticatus Bombyx. The insect-derived medicinal materials were generally used as a combined preparation with other medicinal materials in patents. Conclusion: This study could help to establish the basis for future research and development related treating skin diseases using insect-derived medicinal materials. In order to provide sufficient data, further study including analysis of rejected patents is needed.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.78-83
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• 2022

There are several subspecies of Bombyx mori, whose silk sericin variants differ. Silk sericin can induce bone morphogenic protein-2 (BMP-2) in macrophages, and silk sericin from different species may have different levels of BMP-2 induction ability. In this study, silk sericin from three B. mori subspecies (Baegokjam, Yeonnokjam, and Goldensilk) was prepared. They were administered to RAW264.7 cells and BMP-2 expression level was studied. Bone regeneration was evaluated using a rat calvarial defect model. BMP-2 expression level was the highest in the Baegokjam group. The bone volume in the Baegokjam group was significantly higher than that in the Yeonnokjam group (P = 0.003). In conclusion, sericin from Baegokjam showed higher levels of BMP-2 expression and bone regeneration than those from Yeonnokjam and Goldensilk.