• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.5 no.1
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• pp.47-53
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• 2001

A problem of approximation by irrational splines is considered. These splines have a constant curvature between interpolation nodes and need only one additional boundary condition for derivatives, which should be set only at one of two boundary nodes, that is impossible for usual polynomial splines required boundary conditions at both boundary nodal points. Some estimations for numerical differentiation and rounding error analysis are presented.

• International Journal of Industrial Entomology and Biomaterials
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• v.27 no.1
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• pp.159-165
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• 2013

Bone morphogenetic proteins (BMPs) belong to the transforming growth factor (TGF-${\beta}$) superfamily and are involved in osteoblastic differentiation. The largest TGF-${\beta}$ superfamily subgroup shares genetic homology with human BMPs (hBMPs) and silkworm decapentaplegic (dpp). In addition, hBMPs are functionally interchangeable with Drosophila dpp. Bombyx mori dpp may induce bone formation in mammalian cells. To test this hypothesis, we synthesized the 1,285-base pairs cDNA of full-length B. mori dpp using total RNAs obtained from the fat body of 3-day-old of the $5^{th}$ instar larvae and cloned the cDNA into the pCEP4 mammalian expression vector. Next, B. mori dpp was expressed in C3H10T1/2 cells. The target cells transfected with the pCEP4-Bm dpp plasmid showed biological functions similar to those of osteogenic differentiation induction growth factors such as hBMPs. We determined the relative mRNA expression rates of Runt-related transcription factor 2 (RUNX2), osterix, osteocalcin, and alkaline phosphatase (ALP) to validate the osteoblast-specific differentiation effects of B. mori dpp by performing quantitative real-time RT-PCR. Interestingly, mRNA expression levels of the 3 marker genes except RUNX2, in cells expressing B. mori dpp were much higher than those in control cells and C3H10T1/2 cells transfected with pCEP4. These results suggested that B. mori dpp signaling regulates osterix expression during osteogenic differentiation via RUNX2-independent mechanisms.

• Current Optics and Photonics
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• v.1 no.4
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• pp.412-420
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• 2017

Multinucleated bone resorptive osteoclasts differentiate from bone marrow-derived monocyte/macrophage precursor cells. During osteoclast differentiation, mononuclear pre-osteoclasts change their morphology and biochemical characteristics. In this study, Raman spectroscopy with multivariate techniques such as Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were used to extract biochemical information related to various cellular events during osteoclastogenesis. This technique allowed for label-free and noninvasive monitoring of differentiating cells, and clearly discriminated four different time points during osteoclast differentiation. The Raman band intensity showed significant time-dependent changes that increased up to day 4. The results of Raman spectroscopy agreed with results from atomic force microscopy (AFM) and tartrate-resistant acid phosphatase (TRAP) staining, a conventional biological assay. Under AFM, normal spindle-like mononuclear pre-osteoclasts became round and smaller at day 2 after treatment with a receptor activator of nuclear $factor-{\kappa}B$ ligand and they formed multinucleated giant cells at day 4. Thus, Raman spectroscopy, in combination with PCA-LDA, may be useful for noninvasive label-free quality assessment of cell status during osteoclast differentiation, enabling more efficient optimization of the bioprocesses.

• Industry Promotion Research
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• v.7 no.2
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• pp.31-42
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• 2022

This study was conducted in-depth interviews and examinations by selecting mothers with low levels of self-differentiation and experiencing anxiety about parenting among mothers with low levels of self-differentiation, and analyzed them around categories and analyzed them into general structural techniques. As a current period task, it is the cause of the rasing-anxiety of parenthood in school age and the number of children due to nuclear familyization, and by generalizing the family's problems and experiences of parenting insolvency into categories, it is the reason for recognizing problems such as the child's social response and emotional support and the cause of the mother's low level of self-differentiation and anxiety, and setting the correct direction for parenting.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.9
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• pp.3358-3365
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• 2010

This study was carried out to investigate the effect of Sorbus commixta (SC), Geranium thunbergii (GT) and their mixture (SC:GT=1:1, MIX) on inhibition of bone loss and chondral defect. To examine their activities, we measured the alkaline phosphatase (ALP) activity in human osteoblast-like MG-63 cells and performed tartrate-resistant acid phosphate (TRAP) staining in osteoclast differentiated from Raw264.7 cells. To investigate the influence on chondrocyte differentiation, we performed alcian-blue staining in chondrocyte differentiated from ATDC5 cells. All of SC, GT and MIX did not increase ALP activity in MG-63 cells. However, SC and mixture (SC:GT=1:1, MIX) significantly inhibited osteoclastic differentiation. And they also induced chondrocyte differentiation. These results suggest that SC and GT may have a potential for the treatment of bone loss and chondral defect by suppression of osteoclast differentiation and stimulation of chondrocyte differentiation. Therefore, clarification of their mechanisms and active components will be needed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.1
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• pp.52-63
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• 2019

Biased performance implications are common in conducting empirical analysis on leading firms in strategic management field. Venture businesses in which successful and failing firms in the future are mixed in the present could provide a better discriminative result for examining the determinants of performance. We suggest hypotheses on how competitive strategy, CEO characteristics, and their interactions affect firm performance. We examine these hypotheses through empirical analyses on the basis of a survey collected from 387 venture businesses in order to assure the validity of strategic management theories by using more deviated data. Empirical results show that innovative differentiation from competitive strategies affects firm performance and innovativeness and long-term orientation from CEO characteristics affect firm performance. According to the results of the interaction analyses between competitive strategies and CEO characteristics, cost-leadership and marketing differentiation strategies have combination effects with risk taking, innovativeness, and long-term orientation, while innovative differentiation strategy has no combination effects with risk taking, innovativeness, and long-term orientation. We conclude that cost-leadership and marketing differentiation strategies have no direct effect on firm performance but have combination effects with CEO characteristics, while innovative differentiation strategy has direct effect on firm performance but has no combination effect. Our primary contribution is that we test and confirm that the fit between competitive strategies and CEO characteristics are an important consideration to increase firm performance in venture businesses.

• Journal of Industrial Convergence
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• v.21 no.5
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• pp.15-29
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• 2023

The purpose of this study was intended to analyze the effect of the level of self-differentiation in the financial companies on customer orientation, and to find out the mediating effect of job stress between self-differentiation and customer orientation. In addition, this study was intended to suggest implications that can be used for customer management and marketing activities of financial companies. The results of this study are as follows: First, a low level of fusion with others was found to have a negative(-) effect on customer orientation. second, a level of emotional-cutoff did not affect customer orientation. third, a low level of emotional-reactivity had a positive(+) effect on customer orientation. fourthly, a high level of 'I'-positon has a positive(+) effect on customer orientation. Fifthly, job stress was found to partially mediate between customer orientation and fusion with others, emotional-reactivity, 'I'-positon. This study has academic significance in that it identified patterns of customer orientation according to the level of self-differentiation of employees in the financial companies. As for practical implications, in order to improve customer orientation in employees in the financial companies, first, job stress of organizational members must be reduced. second, members with a low level of emotional-reactivity and a high level of 'I'-positon should be assigned to sales positions, and third, there is a need to assign a lot of authority and responsibility to employees in middle and lower positions.

• Plant Biotechnology Reports
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• v.3 no.4
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• pp.333-340
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• 2009

Developmental anomalies in the plumule meristem of peanut (Arachis hypogaea L.) somatic embryos resulted in poor shoot differentiation and reduced plant recovery. Existing meristems with caulogenic potential have never been tested for embryogenesis in peanut. The present experiment was designed to test the mature zygotic embryo axis derived plumule with three meristems for somatic embryogenesis. Embryogenic masses and embryos developed from the caulogenic meristems in the axils. Exposure of 2 weeks in primary medium with $90.5{\mu}M$ 2,4-D suppressed the shoot tip differentiation temporarily which then regained the ability to form the shoot on withdrawal of 2,4-D. Exposure of 4 weeks in primary medium with $90.5{\mu}M$ 2,4-D suppressed the shoot tip differentiation irreversibly. No shoot formation was noted from the tips in any of the cultures which were in secondary medium with $13.6{\mu}M$ 2,4-D. Development of somatic embryos directly from axillary meristems was confirmed histologically. Conversion frequency of these embryos was 11%. Thus, in this report, we describe a method to obtain somatic embryos from the determined organogenic buds of the axillary meristem, by culturing the nodal explant vertically on embryo induction medium. It also displays the possibility of obtaining both embryogenic and organogenic potential in two parts of the same explant simultaneously. The possibility of extending this approach for genetic transformation in in vivo system through direct DNA delivery or Agrobacterium injection in meristems can also be explored. Using Agrobacterium rhizogenes, we have demonstrated the possibility of gene transfer in the axillary meristems of seed-derived plumule explant.

• Journal of the Korea Convergence Society
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• v.9 no.3
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• pp.181-186
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• 2018

Car-sharing services are one of the successful model of sharing economies. The car-sharing service is a kind of car rental service that can pay the cost per minutes, also can makes it easy to book and return by smart phone at any time. Experts predict that the car-sharing services will likely dominate the auto market in the future by reducing the burden on consumers' purchasing cost of car and resolving the environmental issues caused by the vehicle. Therefore, a differentiated service strategy is needed to establish a competitive caching service among these companies. In this study, we surveyed differentiation cases by comparing and analyzing domestic caching companies, And customized service tailored to the situation and effective vehicle type infotainment. As a result of this study, the proposed service method change, customized service provision, and new platform application are expected to be detailed and in depth.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.9
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• pp.4183-4191
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• 2011

Considering the characteristics of a data stream whose data elements are continuously generated and may change over time, there have been many techniques to differentiate the importance of data elements in a data stream by their generation time. The conventional techniques are efficient to get an analysis result focusing on the recent information in a data stream, but they have a limitation to differentiate the importance of information in various ways more flexible. An information differentiation technique based on the term of a fuzzy set can be an alternative way to compensate the limitation. A term of a fuzzy set has been widely used in various data mining fields, which can overcome the sharp boundary problem and give an analysis result reflecting the requirements in real world applications more. In this paper, a fuzzy window mechanism is proposed, which is adapting a term of a fuzzy set and is efficiently used to differentiate the importance of information in mining data streams. Basic concepts including fuzzy calendars are described first, and subsequently details on data stream mining of weighted patterns using a fuzzy window technique are described.