• Journal of Photoscience
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• 제9권2호
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• pp.90-93
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• 2002

Phytochrome A (phyA) and phytochrome B (phyB) perceive light and adapt to fluctuating circumstances by different manners in terms of effective wavelengths, required fluence and photoreversibility. Action spectra for induction of seed germination and inhibition of hypocotyl elongation using phytochrome mutants of Arabidopsis showed major difference. PhyA is the principal photoreceptor for the very low fluence responses and the far-red light-induced high irradiance responses, while phyB controls low fluence response in a red/far-red reversible mode. The structural requirement of their bilin chromophores for photosensory specificity of phyA and phyB was investigated by reconstituting holophytochromes through feeding various synthetic bilins to the following chromophore-deficient mutants: hy1, hyl/phyA and hyl/phyB mutants of Arabidopsis. We found that the vinyl side-chain of the D-ring in phytochromobilin interacts with phyA apoprotein. This interaction plays a direct role in mediating the specific photosensory function of phyA. The ethyl side-chain of the D-ring in phycocyanobilin fails to interact with phyA apoprotein, therefore, phyA specific photosensory function is not observed. In contrast, both phytochromobilin and phycocyanobilin interact with phyB apoprotein and induce phyB specific photosensory functions. Structural requirements of the apoproteins and the chromophores for the specific photoperception of phyA and phyB are discussed.

• Journal of Korean Dental Science
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• 제8권2호
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• pp.74-81
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• 2015

Purpose: In osteoclast differentiation, actin-rich membrane protrusions play a crucial role in cell adhesion. Odontogenic ameloblast-associated protein (Odam) contributes to cell adhesion by inducing actin rearrangement. Odam-mediated RhoA activity may play a significant role in multinucleation of osteoclasts. However, the precise function of Odam in osteoclast cell adhesion and differentiation remains largely unknown. Here, we identify a critical role for Odam in inducing osteoclast adhesion and differentiation. Materials and Methods: The expression of Odam in osteoclasts was evaluated by immunohistochemistry. Primary mouse bone marrow and RAW264.7 cells were used to test the cell adhesion and actin ring formation induced by Odam. Result: Odam was expressed in osteoclasts around alveolar bone. Odam transfection induced actin filament rearrangement and cell adhesion compared with the control or collagen groups. Overexpression of Odam promoted actin stress fiber remodeling and cell adhesion, resulting in increased osteoclast fusion. Conclusion: These results suggest that Odam expression in primary mouse osteoclasts and RAW264.7 cells promotes their adhesion, resulting in the induction of osteoclast differentiation.

• 한국항공우주학회지
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• 제44권5호
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• pp.431-438
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• 2016

본 논문은 자가발전형 쿼드콥터를 설계하고 제작한 내용을 다룬다. 본 논문에서 제안하는 자가발전형 쿼드콥터는 전자기유도원리를 이용해 설계된 두 가지 발전장치를 통해 추가 전력을 얻는다. 첫 번째 발전장치는 상용 BLDC모터와 프로펠러를 결합해 함께 회전시켜, BLDC모터를 발전기처럼 활용해 전력을 생산하는 장치이다. 두 번째 발전장치는 프로펠러의 회전축에 작은 자석들을 포함한 프레임을 결합해 함께 회전시켜 코일이 감긴 보호링에서 전력을 생산하는 장치이다. 본 발전장치들은 상용 쿼드콥터 프로펠러 하단에 장착되어 실용적인 발전을 할 수 있으리라 기대된다.

• The Korean Journal of Physiology and Pharmacology
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• 제5권1호
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• pp.87-92
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• 2001

Carbon monoxide (CO) binds to soluble guanylate cyclase to lead its activation and elicits smooth muscle relaxation. The vascular tissues have a high capacity to produce CO, since heme oxygenase-2 (HO-2) is constitutively expressed in endothelial and smooth muscle cells, and HO-1 can be greatly up-regulated by oxidative stress. Moreover, the substrate of HO, heme, is readily available for catalysis in vascular tissue. Although the activation of heme oxygenase pathway under various stress conditions may provide a defence mechanism in compromised tissues, the specific role of HO-1-derived CO in the control of aortic contractility still remains to be elucidated. The present study was done to determine the effect of HO-1 induction on the aortic contractility. Thus, the effects of incubation of aortic tissue with S-nitroso-N-acetylpenicillamine (SNAP) for 1 hr on the aortic contractile response to phenylephrine were studied. The preincubation with SNAP resulted in depression of the vasoconstrictor response to phenylephrine. This effect was restored by HO inhibitor or methylene blue but not by NOS inhibitor. The attenuation of vascular reactivity by preincubation with SNAP was also revealed in endothelium-free rings. $AlF4^--evoked$ contraction in control did not differ from that in SNP-treated group. These results suggest that increased production of CO was responsible for the reduction of the contractile response to phenylephrine in aortic ring preincubated with SNAP and this effect of SNAP was independent on endothelium.

• Asian-Australasian Journal of Animal Sciences
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• 제30권8호
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• pp.1183-1189
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• 2017

Objective: Owing to the public availability of complete genome sequences, including avian species, massive bioinformatics analyses may be conducted for computational gene prediction and the identification of gene regulatory networks through various informatics tools. However, to evaluate the biofunctional activity of a predicted target gene, in vivo and in vitro functional genomic analyses should be a prerequisite. Methods: Due to a lack of quail genomic sequence information, we first identified the partial genomic structure and sequences of the quail SH3 domain containing ring finger 2 (SH3RF2) gene. Subsequently, SH3RF2 was knocked out using clustered regularly interspaced short palindromic repeat/Cas9 technology and single cell-derived SH3RF2 mutant sublines were established to study the biofunctional activity of SH3RF2 in quail myoblast (QM7) cells during muscle differentiation. Results: Through a T7 endonuclease I assay and genotyping analysis, we established an SH3RF2 knockout (KO) QM7#4 subline with 61 and 155 nucleotide deletion mutations in SH3RF2. After the induction of myotube differentiation, the expression profiles were analyzed and compared between regular QM7 and SH3RF2 KO QM7#4 cells by global RNA sequencing and bioinformatics analysis. Conclusion: We did not detect any statistically significant role of SH3RF2 during myotube differentiation in QM7 myoblast cells. However, additional experiments are necessary to examine the biofunctional activity of SH3RF2 in cell proliferation and muscle growth.

• 한국재료학회지
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• 제23권9호
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• pp.483-488
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• 2013

Over the past few decades, high-nitrogen austenitic steels have steadily received greater attention since they provide a unique combination of high strength and ductility, good corrosion resistance, and non-magnetic properties. Recently, highnitrogen 18Mn-18Cr austenitic steels with enhanced strength have been developed and widely used for generator retaining rings in order to prevent the copper wiring from being displaced by the centrifugal forces occurring during high-speed rotation. The high-nitrogen austenitic steels for generator retaining ring should be expanded at room temperature and then stress relief annealed at around $400^{\circ}C$ to achieve the required mechanical properties. In this study, four kinds of high-nitrogen 18Mn-18Cr austenitic steels with different nitrogen content were fabricated by using a pressurized vacuum induction melting furnace, and then the effects of nitrogen content, cold working, and stress relieving on tensile properties were investigated. The yield and tensile strengths increased proportionally with increasing nitrogen content and cold working, and they further increased after stress relieving treatment. Based on these results, a semi-empirical equation was proposed to predict the tensile strength of highnitrogen 18Mn-18Cr austenitic steels for generator retaining rings. It will be a useful for the effective fabrication of high-nitrogen 18Mn-18Cr austenitic steels for generator retaining rings with the required tensile properties.

• Bulletin of the Korean Chemical Society
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• 제34권9호
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• pp.2640-2644
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• 2013

The all-hydrocarbon i,i+4 stapling system using an oct-4-enyl crosslink is one of the most widely employed chemical tools to stabilize an ${\alpha}$-helical conformation of a short peptide. This crosslinking system has greatly extended our ability to modulate intracellular protein-macromolecule interactions. The helix-inducing property of the i,i+4 staple has shown to be highly dependent on the length and the stereochemistry of the oct-4-enyl crosslink. Here we show that changing the double bond position within the i,i+4 staple has a considerable impact not only on the formation of the crosslink but also on ${\alpha}$-helix induction. The data further increases the understanding of the structure-activity relationships of this valuable chemical tool.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1992년도 춘계학술대회
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• pp.115-117
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• 1992

고주파 자장 유도를 사용하는 공막 탐지 코일(scleral search coil) 법에 의한 안구 운동의 측정은 분해능, DC 안정성의 장점때문에 정밀 측정의 대표적인 기법으로 정착되었다. 본 연구에서는 공막 탐지 코일법의 한 변형으로서 비교적 간단한 자장 구동 및 탐지회로를 채택하였고 보다 논은 착용감을 주도록 고안된 금박 공막테를 사용하여 안구 운동을 측정한 결과에 관한 것이다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2002년도 하계학술대회 논문집
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• pp.168-171
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• 2002

A SSIMT(Suppressed Sidewall Injection Magnetotransistor) sensor with high linearity is presented in this thesis. The prototype is fabricated by using the Hynix 0.6$\mu\textrm{m}$ P-substrate twin-well double poly three-metal CMOS Process. The fabricated SSIMT shows that variation of the collector current is extremely linear by varing the magnetic induction from -200mT to 200mT at I$\_$B/=500${\mu}$A, V$\_$CE/=2V and V$\_$SUB/=5V. The relative sensitivity is up to 120%/T. At B = 0, magnetic offset is about 79mT, there relative sensitivity is 30.5%/T. The nonlinearity of the fabricated SSIMT is measured about 1.4%.

• Journal of Electrical Engineering and Technology
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• 제6권3호
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• pp.369-374
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• 2011

An induction motor operated with high voltage source generally generates high current in starting mode and has a long transient time after being started. This large and sustaining starting current causes the end windings of the stator to have excessive electromagnetic force. This force is the source of vibration and has a negative and serious influence on the insulation of end windings. Therefore, designing the end winding part with an appropriate support system is needed. To design the support ring enclosing the end windings, we analyze the distribution of electromagnetic force on the end windings by applying the Biot-Savart's law and the 3D finite element method (FEM), and comparing two simulation methods. Finally, we verify the safety of the support structure of the end winding part using stress analysis, which is analyzed with the electromagnetic forces from the 3D FEM simulation.