• Journal of Power Electronics
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• v.11 no.4
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• pp.418-423
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• 2011

This paper presents a simulation model and a parameter identification scheme of an induction motor drive for electric vehicle. The induction motor in automotive applications should operate in very high efficiency and achieve the maximum-torque-per-ampere (MTPA) feature even with saturated magnetic flux under very high torque. The indirect vector control which is typically adopted in traction drive system requires precise information of motor parameters, particularly rotor time constants. This work models an induction motor considering magnetic saturation and proposes an empirical identification method using the current controller in the synchronous reference frame. The proposed method is applied to a 22kW-rated induction motor for electric vehicle.

• The Transactions of the Korean Institute of Power Electronics
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• v.2 no.1
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• pp.39-45
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• 1997

In this paper, a high speed motor drive system using an indirect adaptive neural network controller is proposed. In the variable high speed motor drives, the speed response can be deteriorated by long settling time and high overshoot. To obtain a good dynamical performance, an adaptive feedforward controller consisted of Neural Network Controller(NNC) and Neural Network Emulator(NNE) is applied. The NNE is used to identify the parameters and characteristics of high speed motor. To train the controller, the weights are dynamically adjusted using the back propagation algorithm. Computer simulation and implementation of the proposed system is described.

• Journal of Power Electronics
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• v.13 no.5
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• pp.806-813
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• 2013

This paper aims to compare the performance of three phase induction motor drives using Five Leg Inverter (FLI) and Three Leg Inverter (TLI) configurations. An Indirect Field Oriented Control (IFOC) method using a TLI is well established and incorporated for high performance speed drives in various industries. The FLI dual motor drive system on the other hand shows good workability in the independent control of two induction motor drives simultaneously. In this experiment, the IFOC method is utilized for both drive systems, and Space Vector Pulse Width Modulation (SVPWM) is used to generate pulses for both inverters. For the FLI, the Double Zero Sequence (DZS) Injection technique is used to generate the modulation signal. The complete experiment setup is done by using a DSpace 1103 controller board. The individual motor performances are analyzed using similar schemes, equipment setups and controller parameter values. The results show similar speed performance response capability between the single motor operation using a TLI system and the two motor operation using a FLI system based on the variable speed range either in forward or reverse operation. They also show similar load rejection abilities. However, the single motor with a TLI has a better power quality aspect such as ripple current and total harmonics distortion (THD).

• The Plant Pathology Journal
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• v.40 no.2
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• pp.139-150
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• 2024

Huanglongbing (HLB) is a disease caused by the phloem-limited Candidatus Liberibacter asiaticus (CLas) that affects the citrus industry worldwide. To date, only indirect strategies have been implemented to eradicate HLB. Included among these is the population control of the psyllid vector (Diaphorina citri), which usually provides inconsistent results. Even though strategies for direct CLas suppression seem a priori more promising, only a handful of reports have been focused on a confrontation of the pathogen. Recent developments in polymer chemistry have allowed the design of polycationic self-assembled block copolymers with outstanding antibacterial capabilities. Here, we report the use of polymeric nano-sized bactericide particles (PNB) to control CLas directly in the phloem vasculature. The field experiments were performed in Rioverde, San Luis Potosí, and is one of the most important citrusproducing regions in Mexico. An average 52% reduction in the bacterial population was produced when PNB was injected directly into the trunk of 20 infected trees, although, in some cases, reduction levels reached 97%. These results position PNB as a novel and promising nanotechnological tool for citrus crop protection against CLas and other related pathogens.

• Journal of Veterinary Science
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• v.24 no.3
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• pp.38.1-38.12
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• 2023

Background: Poor disease management and irregular vector control could predispose sheltered animals to disease such as feline Bartonella infection, a vector-borne zoonotic disease primarily caused by Bartonella henselae. Objectives: This study investigated the status of Bartonella infection in cats from eight (n = 8) shelters by molecular and serological approaches, profiling the CD4:CD8 ratio and the risk factors associated with Bartonella infection in shelter cats. Methods: Bartonella deoxyribonucleic acid (DNA) was detected through polymerase chain reaction (PCR) targeting 16S-23S rRNA internal transcribed spacer gene, followed by DNA sequencing. Bartonella IgM and IgG antibody titre, CD4 and CD8 profiles were detected using indirect immunofluorescence assay and flow cytometric analysis, respectively. Results: B. henselae was detected through PCR and sequencing in 1.0% (1/101) oral swab and 2.0% (1/50) cat fleas, while another 3/50 cat fleas carried B. clarridgeiae. Only 18/101 cats were seronegative against B. henselae, whereas 30.7% (31/101) cats were positive for both IgM and IgG, 8% (18/101) cats had IgM, and 33.7% (34/101) cats had IgG antibody only. None of the eight shelters sampled had Bartonella antibody-free cats. Although abnormal CD4:CD8 ratio was observed in 48/83 seropositive cats, flea infestation was the only significant risk factor observed in this study. Conclusions: The present study provides the first comparison on the Bartonella spp. antigen, antibody status and CD4:CD8 ratio among shelter cats. The high B. henselae seropositivity among shelter cats presumably due to significant flea infestation triggers an alarm of whether the infection could go undetectable and its potential transmission to humans.

• Parasites, Hosts and Diseases
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• v.51 no.2
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• pp.147-154
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• 2013

To control coccidiosis without using prophylactic medications, a DNA vaccine targeting the gametophyte antigen Gam56 from Eimeria maxima in chickens was constructed, and the immunogenicity and protective effects were evaluated. The ORF of Gam56 gene was cloned into an eukaryotic expression vector pcDNA3.1(zeo)+. Expression of Gam56 protein in COS-7 cells transfected with recombinant plasmid pcDNA-Gam56 was confirmed by indirect immunofluorescence assay. The DNA vaccine was injected intramuscularly to yellow feathered broilers of 1-week old at 3 dosages (25, 50, and $100{\mu}g/chick$). Injection was repeated once 1 week later. One week after the second injection, birds were challenged orally with $5{\times}10^4$ sporulated oocysts of E. maxima, then weighed and killed at day 8 post challenge. Blood samples were collected and examined for specific peripheral blood lymphocyte proliferation activity and serum antibody levels. Compared with control groups, the administration of pcDNA-Gam56 vaccine markedly increased the lymphocyte proliferation activity (P<0.05) at day 7 and 14 after the first immunization. The level of lymphocyte proliferation started to decrease on day 21 after the first immunization. A similar trend was seen in specific antibody levels. Among the 3 pcDNA-Gam56 immunized groups, the median dosage group displayed the highest lymphocyte proliferation and antibody levels (P<0.05). The median dosage group had the greatest relative body weight gain (89.7%), and the greatest oocyst shedding reduction (53.7%). These results indicate that median dosage of DNA vaccine had good immunogenicity and immune protection effects, and may be used in field applications for coccidiosis control.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.43.1-43.1
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• 2011

The detail simulation modeling of fully-fed induction generator is investigated through PC based MATLAB/Simulink environment. Generator's stator currents are controlled by indirect vector control method. In this method, generator side converter controls the maximum excitation (air gap flux) by stator d-axis current and controls generator torque by stator q-axis current. Induction generator speed is controlled by tip speed ratio (TSR) upon the wind speed variations in order to generate the maximum output power. The generator torque model is specified as a 3-blade wind turbine with rating, then, the model is simulated under normal operating condition and three different fault conditions. The matlab model designed for fully-fed induction generator based wind farm provides good performance under normal and grid fault conditions. It provides good results for different pwm techniques and fault conditions except the single-phase line to ground fault, which should be verified with real time data from wind farms.

• Journal of Korea Water Resources Association
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• v.45 no.7
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• pp.729-741
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• 2012

Among the methods of precipitation data acquisition, a rain gauge station has a distinctive advantage of direct measurement of rainfall itself, but multiple stations should be installed in order to obtain areal precipitation data required for hydrological analysis. On the other hand, a rainfall radar may provide areal distribution of rainfall in real time though it is an indirect measurement of radar echoes on rain drops. Rainfall radars have been shown useful especially for forecasting short-term localized torrential storms that may cause catastrophic flash floods. CAPPI (Constant Altitude Plan Position Indicator), which is one of the several types of radar rainfall image data, has been provided on the Internet in real time by Korea Meteorological Administration (KMA). It is one of the most widely available rainfall data in Korea with fairly high level of confidence as it is produced with bias adjustment and quality control procedures by KMA. The objective of this study is to develop an improved way to extract quantitative rainfall data applicable to even very small watersheds from CAPPI using CIVCOM, which is a new image processing method based on a vector-based scheme proposed in this study rather than raster-based schemes proposed by other researchers. This study shows usefulness of CIVCOM through comparison of rainfall data produced by image processing methods including traditional raster-based schemes and a newly proposed vector-based one.

• Tuberculosis and Respiratory Diseases
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• v.67 no.2
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• pp.95-104
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• 2009

Background: The pathophysiologic mechanisms of early acute lung injury (ALI) differ according to the type of primary insult. It is important to differentiate between direct and indirect pathophysiologic pathways, and this may influence the approach to treatment strategies. NF-$\kappa$B decoy oligodeoxynucleotide (ODN) is a useful tool for the blockade of the expression of NF-$\kappa$B-dependent proinflammatory mediators and has been reported to be effective in indirect ALI. The purpose of this study was to investigate the effect of NF-$\kappa$B decoy ODN in the lipopolysaccharide (LPS)-induced direct ALI model. Methods: Five-week-old specific pathogen-free male BALB/c mice were used for the experiment. In the preliminary studies, tumor necrosis factor (TNF)-$\alpha$, interleukine (IL)-6 and NF-$\kappa$B activity peaked at 6 hours after LPS administration. Myeloperoxidase (MPO) activity and ALI score were highest at 36 and 48 hours, respectively. Therefore, it was decided to measure each parameter at the time of its highest level. The study mice were randomly divided into three experimental groups: (1) control group which was administered 50 ${\mu}L$ of saline and treated with intratracheal administration of 200 ${\mu}L$ DW containing only hemagglutinating virus of Japan (HVJ) vector (n=24); (2) LPS group in which LPS-induced ALI mice were treated with intratracheal administration of 200 ${\mu}L$ DW containing only HVJ vector (n=24); (3) LPS+ODN group in which LPS-induced ALI mice were treated with intratracheal administration of 200 ${\mu}L$ DW containing 160 ${\mu}g$ of NF-$\kappa$B decoy ODN and HVJ vector (n=24). Each group was subdivided into four experimental subgroups: (1) tissue subgroup for histopathological examination for ALI at 48 hours (n=6); (2) 6-hour bronchoalveolar lavage (BAL) subgroup for measurement of TNF-$\alpha$ and IL-6 in BAL fluid (BALF) (n=6); (3) 36-hour BAL subgroup for MPO activity assays in BALF (n=6); and (4) tissue homogenate subgroup for measurement of NF-$\kappa$B activity in lung tissue homogenates at 6 hours (n=6). Results: NF-$\kappa$B decoy ODN treatment significantly decreased NF-$\kappa$B activity in lung tissues. However, it failed to improve the parameters of LPS-induced direct ALI, including the concentrations of tumor necrosis factor-$\alpha$ and interleukin-6 in BALF, myeloperoxidase activity in BALF and histopathologic changes measured by the ALI score. Conclusion: NF-$\kappa$B decoy ODN, which has been proven to be effective in indirect models, had no effect in the direct ALI model.

• Journal of Microbiology and Biotechnology
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• v.32 no.9
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• pp.1120-1125
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• 2022

Japanese encephalitis virus (JEV), the causative agent of Japanese encephalitis (JE), is an importantly zoonotic, vector-borne virus widely prevalent in Asia. Although JE has been well controlled in China, its prevalence remains a huge threat to the pig industry as well as human health. Herein, we report on our molecular and serological investigations of JEV among pigs from different regions in Hunan Province of China from 2019 to 2021. Collectively, 19.27% (583/3026, 95% Confidential Interval (CI) 17.86-20.68) of sampled pigs were positive for JEV IgG antibody as revealed by indirect enzyme-linked immunosorbent assay, and the seroprevalence of JEV among pigs was significantly associated with the development stage and breeding scale (p < 0.01). Meanwhile, 10.99% (42/382, 95% CI 7.86-14.13) of tissue samples of pigs with suspected clinical symptoms of JE and 23.44% (15/64, 95% CI 13.06-33.82) of mosquito batches were JEV-positive via reverse polymerase chain reaction. In addition, the complete E gene sequences of 14 JEV strains identified in this study were amplified and sequenced. Phylogenetic analysis showed that all 14 JEV strains belonged to genotype I-b and displayed a distinct genetic relationship to the present JEV vaccine strain (SA14-14-2). In conclusion, our results revealed not only the severe prevalence of JEV in Hunan Province, but also that JEV I-b might be the predominant genotype in Hunan Province, suggesting therefore that effective measures for JE control are urgently needed.