• Tunnel and Underground Space
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• v.32 no.6
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• pp.411-434
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• 2022

In deep geological disposal concept for spent nuclear fuel, it is well-known that rock mass at near-field experiences the thermal-hydraulic-mechanical (THM) coupled behavior. The mechanical properties of rock changes during the coupled process, and it is important to consider the changes into the analysis of numerical simulation and in-situ tests for long-term stability evaluation of nuclear waste disposal repository. This report collected the previous studies on indirect coupled behaviors of rock. The effects of water saturation and temperature on some mechanical properties of rock was considered, while the change in hydraulic conductivity of rock due to stress was included in the indirect coupled behavior.

• Computers and Concrete
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• v.16 no.4
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• pp.605-623
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• 2015

A coupled experimental and numerical study of shear fracture in the edge-notched beam specimens of quasi-brittle materials (concrete-like materials) are carried out using four point bending flexural tests. The crack initiation, propagation and breaking process of beam specimens are experimentally studied by producing the double inclined edge notches with different ligament angles in beams under four point bending. The effects of ligament angles on the shear fracturing path in the bridge areas of the double edge-notched beam specimens are studied. Moreover, the influence of the inclined edge notches on the shear-fracture behavior of double edge-notched beam specimens which represents a practical crack orientation is investigated. The same specimens are numerically simulated by an indirect boundary element method known as displacement discontinuity method. These numerical results are compared with the performed experimental results proving the accuracy and validity of the proposed study.

• Structural Engineering and Mechanics
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• v.71 no.6
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• pp.699-712
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• 2019

The reinforced concrete lining of hydraulic pressure tunnels tends to crack under high inner water pressure (IWP), which results in the inner water exosmosis along cracks and involves typical hydro-mechanical interaction. This study aims at the development, validation and application of an indirect-coupled method to simulate the lining cracking process. Based on the concrete damage plasticity (CDP) model, the utility routine GETVRM and the user subroutine USDFLD in the finite element code ABAQUS is employed to calculate and adjust the secondary hydraulic conductivity according to the material damage and the plastic volume strain. The friction-contact method (FCM) is introduced to track the lining-rock interface behavior. Compared with the traditional node-shared method (NSM) model, the FCM model is more feasible to simulate the lining cracking process. The number of cracks and the reinforcement stress can be significantly reduced, which matches well with the observed results in engineering practices. Moreover, the damage evolution of reinforced concrete lining can be effectively slowed down. This numerical method provides an insight into the cracking process of reinforced concrete lining in hydraulic pressure tunnels.

• Computers and Concrete
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• v.20 no.1
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• pp.57-63
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• 2017

A coupled experimental-numerical study on shear fracture in concrete specimens with different geometries is carried out. The crack initiation, propagation and final breakage of concrete specimens are experimentally studied under compression loading. The load-strain and the strength of the specimens are experimentally measured, indicating decreasing effects of the shear behavior on the failure load of the specimen. The effects of specimen geometries on the shear fracturing path in the concrete specimens are also investigate. Numerical models using an indirect boundary element method are made to evaluate the crack propagation paths of concrete specimens. These numerical results are compared with the performed experiments and are validated experimentally.

• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.248-254
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• 2008

Candida magnoliae, an osmotolerant and erythritol producing yeast, prefers D-fructose to D-glucose as carbon sources. For the investigation of the fructophilic characteristics with respect to sugar transportation, a sequential extraction method using various detergents and ultracentrifugation was developed to isolate cellular membrane proteins in C. magnoliae. Immunoblot analysis with the Pma1 antibody and two-dimensional electrophoresis analysis coupled with MS showed that the fraction II was enriched with membrane proteins. Eighteen proteins out of 36 spots were identified as membrane or membrane-associated proteins involved in sugar uptake, stress response, carbon metabolism, and so on. Among them, three proteins were significantly upregulated under the fructose supplying conditions. The hexose transporter was highly homologous to Ght6p in Schizosaccharomyces pombe, which was known as a predominant transporter for the fructose uptake of S. pombe because it exhibited higher affinity to D-fructose than D-glucose. The physicochemical properties of the ATP-binding cassette transporter and inorganic transporter explained their direct or indirect associations with the fructophilic behavior of C. magnoliae. The identification and characterization of membrane proteins involved in sugar uptake might contribute to the elucidation of the selective utilization of fructose to glucose by C. magnoliae at a molecular level.