• Title/Summary/Keyword: Indirect Calorimetry

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Correlation between Measured Resting Energy Expenditure and Predicted Basal Energy Expenditure in Female College Students (여대생을 대상으로 한 실측 휴식대사량과 예측 기초대사량의 상관관계에 관한 연구)

  • Chang, Un-Jae;Lee, Kyeong-Ryeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.2
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    • pp.196-201
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    • 2005
  • The aim of this study was to confirm the validity of predictive equations for the calculation of basal energy expenditure (BEE). One hundred twenty female college students were participated in this study. The resting energy expenditure (REE) was measured by indirect calorimetry for 30 minutes following an 12 hour overnight fasting. Among the available equations for predict BEE, Harris-Benedict, WHO/FAO/UNU and Cunningham methods were selected. Body composition was estimated by bioelectrical impedance analysis (BIA) for the equation of predicted BEE. The mean of measured REE was 1257.2$\pm$147.9 kcal/day, while the predicted value by Harris-Benedict, WHO/FAO/UNU and Cunningham were 1373.3$\pm$45.4 kcal/day, 1290.0$\pm$61.7 kcal/day and 1187.6$\pm$49.2 kcal/day, respectively. The Cunningham equation was more closed to measured values than Harris-Benedict and WHO/FAO/UNU equation by the correlation coefficient. Comparing Pearson's correlation coefficients, fat-free mass (FFM), body surface area (BSA) and body weight were higher than others such as height, body mass index (BMI), fat and fat%. The FFM's correlation coefficient was the highest as 0.74. Thus, the conclusion of this study suggested that the main determinant of BEE was FFM, and we derived a prediction equation as follows: BEE=-569.86+48.27 (FFM).

Metabolizable energy requirement for maintenance estimated by regression analysis of body weight gain or metabolizable energy intake in growing pigs

  • Liu, Hu;Chen, Yifan;Li, Zhongchao;Li, Yakui;Lai, Changhua;Piao, Xiangshu;van Milgen, Jaap;Wang, Fenglai
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.9
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    • pp.1397-1406
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    • 2019
  • Objective: Feed energy required for pigs is first prioritized to meet maintenance costs. Additional energy intake in excess of the energy requirement for maintenance is retained as protein and fat in the body, leading to weight gain. The objective of this study was to estimate the metabolizable energy requirements for maintenance ($ME_m$) by regressing body weight (BW) gain against metabolizable energy intake (MEI) in growing pigs. Methods: Thirty-six growing pigs ($26.3{\pm}1.7kg$) were allotted to 1 of 6 treatments with 6 replicates per treatment in a randomized complete block design. Treatments were 6 feeding levels which were calculated as 50%, 60%, 70%, 80%, 90%, or 100% of the estimated ad libitum MEI ($2,400kJ/kg\;BW^{0.60}\;d$). All pigs were individually housed in metabolism crates for 30 d and weighed every 5 d. Moreover, each pig from each treatment was placed in the open-circuit respiration chambers to measure heat production (HP) and energy retained as protein ($RE_p$) and fat ($RE_f$) every 5 d. Serum biochemical parameters of pigs were analyzed at the end of the experiment. Results: The average daily gain (ADG) and HP as well as the $RE_p$ and $RE_f$ linearly increased with increasing feed intake (p<0.010). ${\beta}$-hydroxybutyrate concentration of serum tended to increase with increasing feed intake (p = 0.080). The regression equations of MEI on ADG were MEI, $kJ/kg\;BW^{0.60}\;d=1.88{\times}ADG$, g/d+782 ($R^2=0.86$) and $ME_m$ was estimated at $782kJ/kg\;BW^{0.60}\;d$. Protein retention of growing pigs would be positive while REf would be negative at this feeding level via regression equations of $RE_p$ and $RE_f$ on MEI. Conclusion: The $ME_m$ was estimated at $782kJ/kg\;BW^{0.60}\;d$ in current experiment. Furthermore, growing pigs will deposit protein and oxidize fat if provided feed at the estimated maintenance level.

Prediction of net energy values in expeller-pressed and solvent-extracted rapeseed meal for growing pigs

  • Li, Zhongchao;Lyu, Zhiqian;Liu, Hu;Liu, Dewen;Jaworski, Neil;Li, Yakui;Lai, Changhua
    • Animal Bioscience
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    • v.34 no.1
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    • pp.109-118
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    • 2021
  • Objective: The objective of this study was to determine net energy (NE) of expeller-press (EP-RSM) and solvent-extracted rapeseed meal (SE-RSM) and to establish equations for predicting the NE in rapeseed meal (RSM) fed to growing pigs. Methods: Thirty-six barrows (initial body weight [BW], 41.1±2.2 kg) were allotted into 6 diets comprising a corn-soybean meal basal diet and 5 diets containing 19.50% RSM added at the expense of corn and soybean meal. The experiment had 6 periods and 6 replicate pigs per diet. During each period, the pigs were individually housed in metabolism crates for 16 days which included 7 days for adaption to diets. On day 8, pigs were transferred to respiration chambers and fed their respective diet at 2,000 kJ metabolizable energy (ME)/kg BW0.6/d. Feces and urine were collected, and daily heat production was measured from day 9 to 13. On days 14 and 15, the pigs were fed at 890 kJ ME/kg BW0.6/d and fasted on day 16 for evaluation of fasting heat production (FHP). Results: The FHP of pigs averaged 790 kJ/kg BW0.6/d and was not affected by the diet composition. The NE values were 10.80 and 8.45 MJ/kg DM for EP-RSM and SE-RSM, respectively. The NE value was positively correlated with gross energy (GE), digestible energy (DE), ME, and ether extract (EE). The best fit equation for NE of RSM was NE (MJ/kg DM) = 1.14×DE (MJ/kg DM)+0.46×crude protein (% of DM)-25.24 (n = 8, R2 = 0.96, p<0.01). The equation NE (MJ/kg DM) = 0.22×EE (% of DM)-0.79×ash (% of DM)+14.36 (n = 8, R2 = 0.77, p = 0.018) may be utilized to quickly determine the NE in RSM when DE or ME values are unavailable. Conclusion: The NE values of EP-RSM and SE-RSM were 10.80 and 8.45 MJ/kg DM. The NE value of RSM can be well predicted based on energy content (GE, DE, and ME) and proximate analysis.

Evaluating and predicting net energy value of wheat and wheat bran for broiler chickens

  • Ning, Ran;Cheng, Zichen;Liu, Xingbo;Ban, Zhibin;Guo, Yuming;Nie, Wei
    • Animal Bioscience
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    • v.35 no.11
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    • pp.1760-1770
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    • 2022
  • Objective: It is crucial to accurately determine the net energy (NE) values of feed ingredients because the NE system is expected to be applied to the formulation of broilers feed. The NE values of 5 wheat and 5 wheat brans were determined in 12-to 14-day old Arbor Acres (AA) broilers with substitution method and indirect calorimetry method. Methods: A total of 12 diets, including 2 reference diets (REF) and 10 test diets (5 wheat diets and 5 wheat bran diets) containing 30% of test ingredients, were randomly fed to 864 male AA birds with 6 replicates of 12 birds per treatment. These birds were used to determine metabolizable energy (ME) (8 birds per replicate) in the chicken house and NE (4 birds per replicate) in the chamber respectively at the same time. After a 4-d dietary and environment adaptation period, growth performance, energy values, energy balance and energy utilization were measured during the following 3 d. Multiple linear regression analyses were further performed to generate prediction equations for NE values based on the chemical components and ME values. The NE prediction equation were also validated on another wheat diet and another wheat bran diet with high correlation (r = 0.98, r = 0.75). Results: The NE values of 5 wheat and 5 wheat bran samples are 9.34, 10.02, 10.27, 11.33, and 10.49 MJ/kg, and 5.37, 5.17, 4.87, 5.06, and 4.88 MJ/kg DM, respectively. The equation with the best fit were NE = 1.968AME-0.411×ADF-14.227 (for wheat) and NE = -0.382×CF-0.362×CP-0.244×ADF+20.870 (for wheat bran). Conclusion: The mean NE values of wheat and wheat bran are 10.29 and 5.07 MJ/kg DM in AA broilers. The NE values of ingredients could be predicted by their chemical composition and energy value with good fitness.

Dynamic of heat production partitioning in rooster by indirect calorimetry

  • Rony Lizana, Riveros;Rosiane, de Sousa Camargos;Marcos, Macari;Matheus, de Paula Reis;Bruno Balbino, Leme;Nilva Kazue, Sakomura
    • Animal Bioscience
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    • v.36 no.1
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    • pp.75-83
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    • 2023
  • Objective: The objective of this study was to describe a methodological procedure to quantify the heat production (HP) partitioning in basal metabolism or fasting heat production (FHP), heat production due to physical activity (HPA), and the thermic effect of feeding (TEF) in roosters. Methods: Eighteen 54-wk-old Hy Line Brown roosters (2.916±0.15 kg) were allocated in an open-circuit chamber of respirometry for O2 consumption (VO2), CO2 production (VCO2), and physical activity (PA) measurements, under environmental comfort conditions, following the protocol: adaptation (3 d), ad libitum feeding (1 d), and fasting conditions (1 d). The Brouwer equation was used to calculate the HP from VO2 and VCO2. The plateau-FHP (parameter L) was estimated through the broken line model: HP = U×(R-t)×I+L; I = 1 if t<R or I = 0 if t>R; Where the broken-point (R) was assigned as the time (t) that defined the difference between a short and long fasting period, I is conditional, and U is the decreasing rate after the feed was withdrawn. The HP components description was characterized by three events: ad libitum feeding and short and long fasting periods. Linear regression was adjusted between physical activity (PA) and HP to determine the HPA and to estimate the standardized FHP (st-FHP) as the intercept of PA = 0. Results: The time when plateau-FHP was reached at 11.7 h after withdrawal feed, with a mean value of 386 kJ/kg0.75/d, differing in 32 kJ from st-FHP (354 kJ/kg0.75/d). The slope of HP per unit of PA was 4.52 kJ/mV. The total HP in roosters partitioned into the st-FHP, termal effect of feeding (TEF), and HPA was 56.6%, 25.7%, and 17.7%, respectively. Conclusion: The FHP represents the largest fraction of energy expenditure in roosters, followed by the TEF. Furthermore, the PA increased the variation of HP measurements.

Validation of a physical activity classification table in Korean adults and elderly using a doubly labeled water method (한국 성인과 노인을 대상으로 이중표식수법을 이용한 신체활동분류표 타당도 평가)

  • Hye-Ji Han ;Ha-Yeon Jun;Jonghoon Park;Kazuko Ishikawa-Takata;Eun-Kyung Kim
    • Journal of Nutrition and Health
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    • v.56 no.4
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    • pp.391-403
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    • 2023
  • Purpose: This study evaluated the validity of a physical activity classification table (PACT) based on total energy expenditure (TEE) and physical activity level (PAL) measured using the doubly labeled water (DLW) method in Korean adults and the elderly. Methods: A total of 141 (male 70, female 71) adults and elderly were included. The reference standards TEEDLW, PALDLW were measured over a 14-day period using DLW. A 24-hour physical activity diary was kept for three days (two days during the week and one day on the weekend). PALPACT was calculated by classifying the activity type and intensity using the PACT. PALPACT was multiplied by resting energy expenditure measured by indirect calorimetry to estimate TEEPACT. Results: The mean age of the study participants was 50.5 ± 18.8 years, and the mean body mass index was 23.4 ± 3.3 kg/m2. A comparison of TEEDLW and TEEPACT by sex and age showed no significant differences. The bias, the difference between TEEDLW and TEEPACT, was male 17.3 kcal/day and female -4.5 kcal/day. The percentage of accurate predictions (values within ± 10% of the TEEDLW) of TEEPACT was 58.6% in males and 54.9% in females, with the highest prediction values in the age group 40-64 years (70.9%) in males and over 65 years (73.9%) in females. The spearman correlation coefficient (r) between TEEPACT and TEEDLW was 0.769, indicating a significant positive correlation (p < 0.001). Conclusion: In this study, the use of a new PACT for calculating TEE and PAL was evaluated as valid. A web version of the software program and a smartphone application need to be developed using PACT to make it easier to apply for research purposes.

Energy expenditure of physical activity in Korean adults and assessment of accelerometer accuracy by gender (성인의 13가지 신체활동의 에너지 소비량 및 가속도계 정확성의 남녀비교)

  • Choi, Yeon-jung;Ju, Mun-jeong;Park, Jung-hye;Park, Jong-hoon;Kim, Eun-kyung
    • Journal of Nutrition and Health
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    • v.50 no.6
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    • pp.552-564
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    • 2017
  • Purpose: The purpose of this study was to measure energy expenditure (EE) the metabolic equivalents (METs) of 13 common physical activities by using a portable telemetry gas exchange system ($K4b^2$) and to assess the accuracy of the accelerometer (Actigraph $GT3X^+$) by gender in Korean adults. Methods: A total of 109 adults (54 males, 55 females) with normal BMI (body mass index) participated in this study. EE and METs of 13 selected activities were simultaneously measured by the $K4b^2$ portable indirect calorimeter and predicted by the $GT3X^+$ Actigraph accelerometer. The accuracy of the accelerometer was assessed by comparing the predicted with the measured EE and METs. Results: EE (kcal/kg/hr) and METs of treadmill walking (3.2 km/h, 4.8 km/h and 5.6 km/h) and running (6.4 km/h) were significantly higher in female than in male participants (p < 0.05). On the other hand, the accelerometer significantly underestimated the EE and METs for all activities except descending stairs, moderate walking, and fast walking in males as well as descending stairs in females. Low intensity activities had the highest rate of accurate classifications (88.3% in males and 91.3% females), whereas vigorous intensity activities had the lowest rate of accurate classifications (43.6% in males and 27.7% in females). Across all activities, the rate of accurate classification was significantly higher in males than in females (75.2% and 58.3% respectively, p < 0.01). Error between the accelerometer and $K4b^2$ was smaller in males than in females, and EE and METs were more accurately estimated during treadmill activities than other activities in both males and females. Conclusion: The accelerometer underestimated EE and METs across various activities in Korean adults. In addition, there appears to be a gender difference in the rate of accurate accelerometer classification of activities according to intensity. Our results indicate the need to develop new accelerometer equations for this population, and gender differences should be considered.

The Relationship of the Severity of Sleep Apnea Syndrome to the Resting Energy Expenditure and Leptin (수면무호흡증의 중증도와 안정시 에너지 대사 및 혈중 Leptin과의 관계)

  • Lee, Kwan-Ho;Shin, Kyeong-Cheol;Ahn, Jae-Hee
    • Tuberculosis and Respiratory Diseases
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    • v.46 no.6
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    • pp.836-845
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    • 1999
  • Background : Obesity is present in the majority of adult patients with obstructive sleep apnea(OSA) and is considered to be a major risk factor for its development. A reduction in body weight has been associated with substantial improvement in the severity of apnea. However, a variety of treatment strategies for obesity have yielded limited sucess. This study was done to determine resting energy expenditure(REE) in patients with obstructive sleep apnea and the correlation between the severity of sleep apnea and REE, and to investigate whether leptin influences REE and correlated with the severity of sleep apnea in 39 patients with OSA and 45 controls matched for obesity. Method : Overnight polysomnography was performed on all subjects using standard techniques. Measurements of REE were made using a Sensormedic Vmax 229 and a canopy system. Serum leptin concentration was measured by human leptin RIA kit of LINCO Research INC. Results : REE was greater in patients with OSA compared with controls, but there was no difference between the two groups on REE%. And also there was no significant correlation between anthropometric data, polysomnographic data and REE%. Serum leptin was linearly related to body mass index(BMI), apnea index, apnea hypopnea index and lowest arterial oxygen saturation($SaO_2$) but not related to REE%. Conclusion : This study suggests the followings. Firstly patients patients with sleep apnea have a pattern of obesity characterized by energy homeostasis at an elevated body weight set-point. In order to achieve a lower body weight in these patients, it may be necessary to increase energy expenditure by increasing physical activity. Secondly leptin level was not correlated with REE, suggesting that leptin may predominantly regulate body fat by altering eating behavior rather than calorigenesis. Lastly leptin level was significantly correlated with the severity of sleep apnea. These elevated level of leptin in patients of sleep apnea may be related to the obesity, however it needs further studies to determine the relationship between the severity of sleep apnea and serum leptin.

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Effects of High Carbohydrate, High Fat and Protein Meal on Postprandial Thermogenesis in Young Women (한국인 젊은 여성에서 고당질, 고지방 및 고단백질 식사가 식후 열생성에 미치는 영향)

  • Ro, Hee-Kyong;Choi, In-Seon;Oh, Seung-Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.8
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    • pp.1202-1209
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    • 2005
  • The purpose of this study was to observe the effects of high-carbohydrate (HC) diet, high-protein (HP) diet and high-fat (HF) diet with a normal diet (N) on diet-induced thermogenesis (DIT) in healthy Korean women. The four isoenergetic test meals consisted as follows: HC (75$ \% $ energy from carbohydrate, 10$ \% $ from protein and 15$ \% $ from fat), HP (10$ \% $ energy from carbohydrate, 75$ \% $ from protein and 15$ \% $ from fat) HF (15$ \% $ energy from carbohydrate, 10$ \% $ from protein and 75$ \% $ from fat) diet and N (65$ \% $ energy from carbohydrate, 15$ \% $ from protein and 20$ \% $ from fat) diet. Fasting and postprandial thermogenesis were measured after each test meals in eight subjects by indirect calorimetry for 3 hours. Fasting and postprandial serum glucose, insulin and triacylglycerol contents were also measured for 3 hours. DIT were 10.4$\pm$3.2 for HC, 12.7$\pm$0.5 for HP, 6.9$\pm$2.8 for HF and 8.7$\pm$4.8 for N diet as a percentage of the energy load. DIT of HP was greater than HF significantly, but had no differences with HC and N Integrated areas under the curves ($ \delta- $-AUC) of plasma glucose and insulin were significantly higher for HC compared to HP, HF and N diets. $ \delta$-AUC of plasma triacylglycerol was significantly higher for HF compared to the other test meals. In conclusion, intake of protein rich meal stimulates postprandial energy expenditure, but has no relation between DIT and insulin response, body composition.

Validation of the physical activity classification table for Korean youth and assessment of total energy expenditure, estimated energy requirement and physical activity in Korean children and adolescents (한국 소아청소년을 위한 신체활동분류표의 타당도 평가 및 이를 이용한 일일 총에너지소비량, 에너지필요추정량과 신체활동 평가)

  • Ji-Yeon Gwak;Myung-Hee Kim;Jonghoon Park;Kazuko Ishikawa-Takata;Eun-Kyung Kim
    • Journal of Nutrition and Health
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    • v.56 no.1
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    • pp.35-53
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    • 2023
  • Purpose: The purpose of the first part of this study was to evaluate the validity of the physical activity classification table for youth (Youth-PACT). The second part of this study was aimed at comparing the estimated energy requirement (EER) with the total energy expenditure (TEE) and evaluating the physical activity patterns of Korean children and adolescents. Methods: The subjects of the first part of the study were 17 children aged 10 to 12 years, and their total energy expenditure (TEEDLW) was measured using the double labeled water (DLW) method. A total of 166 children and adolescents aged 6-18 years participated in the second part of this study. Their resting energy expenditure (REE) was measured using indirect calorimetry and the TEEYouth-PACT and physical activity level were calculated by applying the Youth-PACT to the physical activity diary prepared by the subjects. Results: In the first part of this study, there were no significant differences between the TEEDLW and the TEEYouth-PACT. The TEEYouth-PACT accurately predicted TEEDLW in 37.5% of the subjects. In the second part of the study, the rates at which EER accurately predicted TEE YouthPACT and overestimated TEE Youth-PACT were 29.6% and 47.3%, respectively. The time spent based on intensity of physical activity and the physical activity categories which were obtained using Youth-PACT showed different patterns according to sex and age group. Age showed significant positive correlations with REE, TEE, and the time spent in sedentary behavior, but age was significantly negatively correlated with REE/body weight, TEE/body weight, and the time spent in low-intensity and high-intensity activities. Conclusion: The results of this study showed that the Youth-PACT can be used to evaluate the TEE and PAL of children and adolescents. However, further studies are needed to validate the TEEYouth-PACT and to set the EER for children and adolescents.