• Title/Summary/Keyword: Indica Rice

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Identification of Subspecies-specific STS Markers and Their Association with Segregation Distortion in Rice(Oryza sativa L.)

  • Chin, Joong-Hyoun;Kim, Jung-Hee;Jiang, Wenzhu;Chu, Sang-Ho;Woo, Mi-Ok;Han, Longzhi;Brar, Darshan;Koh, Hee-Jong
    • Journal of Crop Science and Biotechnology
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    • v.10 no.3
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    • pp.175-184
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    • 2007
  • Two subspecies, japonica and indica, have been reported in rice, which differ in several ecotypic traits. However, reproductive barriers in hybrid progenies between subspecies have been major obstacles in breeding programs using inter-subspecific hybridization. As the first step to elucidate the reproductive barriers, we developed subspecies-specific(SS) STS markers in this study. A total of 765 STS primers were designed through comparing DNA sequences at every $2{\sim}3$cM interval between japonica and indica rices, which are available at Web DBs such as IRGSP, NCBI, TIGR, and GRAMENE, and tested for subspecies-specificity using 15 indica and 15 japonica varieties of diverse origin. Of them, 67 STS markers were identified as SS STS markers and their subspecies-specificity scores were estimated. The SS markers were dispersed throughout the genome along chromosomes. Of them, 64 SS markers were mapped on an RIL population derived from a Dasanbyeo(indica)/TR22183(japonica) cross. Genomic inclination of RILs was evaluated based on the genotyping with different types of markers. Association test between markers and segregation distortion revealed that segregation distortion might not be the cause of generating SS markers. The SS markers will be applicable to estimate the genomic inclination of varieties or lines and to study the differentiation of indica and japonica, and ultimately to breed true hybrid rice varieties in which desirable characters from both subspecies are recombined.

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Genetic Diversity of Rice Collections using Subspecies-specific STS Markers (아종특이적 STS 마커를 이용한 벼 품종의 유전다양성 분석)

  • Kim, Bong-Song;Jiang, Wenzhu;Koh, Hee-Jong
    • Korean Journal of Breeding Science
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    • v.41 no.2
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    • pp.101-105
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    • 2009
  • Rice (Oryza sativa L.), the world's most important crop, is usually classified into ssp. indica and japonica based on morpho-physiological traits. In the previous study, we have developed subspecies-specific STS markers (SS markers) to readily discriminate between indica and japonica in O. sativa. In this study, we employed SS markers to investigate the genomic inclination of worldwide collections of O. sativa. A total of 320 varieties were divided into two groups with 63 SS markers. Namely, they formed two distinctive groups, indica and japonica, as expected by their geographic origin. The population structure analysis revealed that the variability of genetic background was greater in indica than in japonica. Some of them, however, exhibited intermediate genomic inclination between indica and japonica. These results are in general agreement with the previous studies, suggesting that SS markers are powerful tools for both determination of subspecies genome and assessment of genetic diversity in rice.

Effect of Copper on Callus Formation and Plant Regeneration in Seed Culture of Rice (벼 종자배양에서 Copper가 캘러스 형성 및 식물체 재분화에 미치는 영향)

  • 권용삼;손재근
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.4
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    • pp.205-208
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    • 2001
  • This study was conducted to improve the regeneration efficiency from seed-derived calli of rice by optimizing the copper concentrations in the media. Mature seeds were cultured on MS medium supplemented with copper sulphate (0 to 5.0 mg/L) and 2 mg/L 2,4-D. Callus growth was influenced by the levels of copper sulphate containing with medium, The addition of copper sulphate (2.5 mg/L) in regeneration medium enhanced dramatically the ability of plant regeneration from seed-derived calli. The mean frequency of plant regeneration of 6 indica rices was 27.4% on medium containing copper sulphate, whereas that of the cultivars on copper-free medium was 2.4%. These results suggest that copper sulphate may have an important role in improving regeneration ability of indica rices.

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Comparative Study of Stomatal Density and Gas Diffusion Resistance in Leaves of Various Types of Rice (벼 품종유형간 잎 기공밀도와 기체확산저항 비교)

  • Chen, Wenfu;Su, Zenjin;Qian, Taiyong;Zhang, Longbu;Joo Yeul, Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.2
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    • pp.125-132
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    • 1995
  • Studies were made on differences among types and varieties of rice in stomatal density and gas diffusion resistance, and on the relationship between these traits and photosynthetic rate. Significant differences among types and varieties were found stomatal density and gas diffusion resistance. Generally, stomatal density was higher in indica varieties than in Japonica varieties, gas diffusion resistance was lower in the former than in the later, in varieties developed through indica-japonica hybridization it was intermadiate. The stomatal density was closely positively correlated with the gas conductivity and the net photosynthetic rate, was not correlated with single leaf area, and had significant negative correlation with specific leaf weight. Higher photosynthetic rate of indica varieties mainly results from its high stomatal density and low gas diffusion resistance. The result also suggested that high photosynthetic rate might be obtained if the high stomatal density and low gas diffusion resistance in indica could be combined with the larger specific leaf weight in japonica through crossing between two.

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Chemical Components Related with Seed Dormancy and Viviparous Germination in Rice (벼 종실의 휴면 관련 성분과 휴면성 및 수발아성과의 관계)

  • 김용욱
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.1
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    • pp.113-119
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    • 1995
  • This excperiment was conducted to investigate relation on seed dormancy and vi-viparous germination with sucrose content, fatty acid composition and abscisic acid content of rice seeds. The following results were obtained by this experiment. Sucrose content of brown rice harvested at 20 and 40 days after heading was higher in Japonica and Sare type varieties than in Tongil and Indica type rices. Positive relationship was found be-tween sucrose content of brown rice and viviparous germination rate of rice varieties tested.J aponica and Sare type rice varieties showed low proportion of oleic acid and higher proportion of linoleic acid in brown rice harvested at 20 and 40 days after heading than Tongil and Indica rices tested. Viviparous germination was negatively correlated with the proportion oleic acid and positively with that of linoleic acid. Abscisic acid content in hull of rice seeds stored for 30 days at 25/15$^{\circ}C$ fluctuation temperature condition was decreased more rapidly than 4$^{\circ}C$, and Japonica varieties tested showed lower con-tent of abscisic acid in rice hull than Tongil and Indica varieties used.

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Effect of different seedling ages and transplanting times on growth and yield of Indica × Japonica rice for noodle processing

  • Kim, Sang Yeol;Oh, Seong Hwan;Seo, Jong Ho;Yi, Hwi Jong;Hwang, Chung Dong;Bae, Hyun Kyung;Choi, Won Yeong;Oh, Myung Kyu
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.327-327
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    • 2017
  • The effect of different seedling ages and transplanting times on the growth and yield of Indica ${\times}$ Japonica rice for noodle processing was evaluated to develop a high yielding cultivation technology for increasing the competition against the imported foreign rice. Four seedling ages (10-, 20-, 30- and 33-day old) of two Indica ${\times}$ Japonica rice cultivars (cvs. Saemimyeon and Palbangmi) and three transplanting dates (May 20, May 30 and June 9) were used in the study. Our results showed that the growth and rice yield of the two cultivars were significantly affected by the different seedling ages and transplanting times. Dry matter production at the panicle heading of the two rice cultivars were generally higher in the 30-day old seedling than the other seedling age treatments and then gradually decreased as the transplanting time was delayed from May 20 to June 9. Similar high panicle number per square meter were recorded at the 30-day old seedling between May 20-May 30 transplanting times. In contrast, other yield parameters that includes spikelet number per panicle, 1,000-brown rice weight, and ripened grain ratio (except for the June 9 transplanting time of Palbangmi) were not significantly affected. The milled rice yield of Saemimyeon was higher than that of the Palbangmi regardless of seedling ages and it was also the highest at the 30-day old seedling with four seedling ages. The milled rice yield of Saemimyeon was only slightly decreased as the transplanting time was delayed from the May 20 to June 9 but Palbangmi had a significantly lower milled rice yield at June 9 transplanting due to the low ripened grain ratio. The result indicates that the most suitable seedling age for the cultivars tested was at 30-day old seedling for noodle processing rice and recommended transplanting times were between May 20 and May 30 for the high rice yield in the Yeongnam plain area.

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Comparison of Amylogram Properties among Several Subspecies of Rice (여러 아종 벼 품종들간 아밀로그램 특성 비교)

  • Kwak Tae-Soon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.3
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    • pp.186-190
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    • 2005
  • This experiment was conducted to collect the basic information on the varietal diversity in amylogram properties of 3 different rice sub-species under tropical conditions in IRRI 2001 wet season based upon correlation and principal component analysis. The peak viscosity and breakdown property of Tongil type, i.e.; temperate Indica species showed higher similarity with Japonica type species rather than typical Indica and tropical japonica types. The amylogram properties such as final viscosity, pasting consistency and setback of Tongil type varieties were lower than those of typical Indica and tropical japonica types. The peak viscosity showed positive correlation with trough, while the breakdown showed negative correlation with setback in all tested 3 rice subspecies. The first principal component was applicable to increase the gelatinization temperature, final viscosity, pasting consistency and setback, and applicable to decrease the peak viscosity and breakdown. Varietal classification by the principal component score of each pedigree could be applied to the interpretation of the community by the scatter diagram for the amylogram properties to the different sub-species of rice at IRRI conditions.

Mechanical and Rheological Properties of Rice Plant (수도(水稻)의 역학적(力學的) 및 리올러지 특성(特性)에 관(關)한 연구(硏究))

  • Huh, Yun Kun;Cha, Gyun Do
    • Korean Journal of Agricultural Science
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    • v.14 no.1
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    • pp.98-133
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    • 1987
  • The mechanical and rheological properties of agricultural materials are important for engineering design and analysis of their mechanical harvesting, handling, transporting and processing systems. Agricultural materials, which composed of structural members and fluids do not react in a purely elastic manner, and their response when subjected to stress and strain is a combination of elastic and viscous behavior so called viscoelastic behavior. Many researchers have conducted studies on the mechanical and rheological properties of the various agricultural products, but a few researcher has studied those properties of rice plant, and also those data are available only for foreign varieties of rice plant. This study are conducted to experimentally determine the mechanical and the rheological properties such as axial compressive strength, tensile strength, bending and shear strength, stress relaxation and creep behavior of rice stems, and grain detachment strength. The rheological models for the rice stem were developed from the test data. The shearing characteristics were examined at some different levels of portion, cross-sectional area, moisture content of rice stem and shearing angle. The results obtained from this study were summarized as follows 1. The mechanical properties of the stems of the J aponica types were greater than those of the Indica ${\times}$ Japonica hybrid in compression, tension, bendingand shearing. 2. The mean value of the compressive force was 80.5 N in the Japonica types and 55.5 N in the Indica ${\times}$ Japonica hybrid which was about 70 percent to that of the Japonica types, and then the value increased progressively at the lower portion of the stems generally. 3. The average tensile force was about 226.6 N in the Japonica types and 123.6 N in the Indica ${\times}$ Japonica hybrid which was about 55 percent to that of the Japonica types. 4. The bending moment was $0.19N{\cdot}m$ in the Japonica types and $0.13N{\cdot}m$ in the Indica ${\times}$ Japonica hybrid which was 68 percent to that of the Japonica types and the bending strength was 7.7 MPa in the Japonica types and 6.5 MPa in the Indica ${\times}$ Japonica hybrid respectively. 5. The shearing force was 141.1 N in Jinju, the Japonica type and 101.4 N in Taebaeg, the Indica ${\times}$ Japonica hybrid which was 72 percent to that of Jinju, and the shearing strength of Taebaeg was 63 percent to that of Jinju. 6. The shearing force and the shearing energy along the stem portion in Jinju increased progressively together at the lower portions, meanwhile in Taebaeg the shearing force showed the maximum value at the intermediate portion and the shearing energy was the greatest at the portion of 21 cm from the ground level, and also the shearing strength and the shearing energy per unit cross-sectional area of the stem were the greater values at the intermediate portion than at any other portions. 7. The shearing force and the shearing energy increased with increase of the cross-sectional area of the rice stem and with decrease of the shearing angie from $90^{\circ}$ to $50^{\circ}$. 8. The shearing forces showed the minimum values of 110 N at Jinju and of 60 N at Taebaeg, the shearing energy at the moisture content decreased about 15 percent point from initial moisture content showed value of 50 mJ in Jinju and of 30 mJ in Taebaeg, respectively. 9. The stress relaxation behavior could be described by the generalized Maxwell model and also the compression creep behavior by Burger's model, respectively in the rice stem. 10. With increase of loading rate, the stress relaxation intensity increased, meanwhile the relaxation time and residual stress decreased. 11. In the compression creep test, the logarithmic creep occured at the stress less than 2.0 MPa and the steady-state creep at the stress larger than 2.0 MPa. 12. The stress level had not a significant effect on the relaxation time, while the relaxation intensity and residual stress increased with increase of the stress level. 13. In the compression creep test of the rice stem, the instantaneous elastic modulus of Burger's model showed the range of 60 to 80 MPa and the viscosities of the free dashpot were very large numerical value which was well explained that the rice stem was viscoelastic material. 14. The tensile detachment forces were about 1.7 to 2.3 N in the Japonica types while about 1.0 to 1.3 N in Indica ${\times}$ Japonica hybrid corresponding to 58 percent of Japonica types, and the bending detachment forces were about 0.6 to 1.1 N corresponding to 30 to 50 percent of the tensile detachment forces, and the bending detachment of the Indica ${\times}$ Japonica hybrid was 0.1 to 0.3 N which was 7 to 21 percent of Japonica types. 15. The detachment force of the lower portion was little bigger than that of the upper portion in a penicle and was not significantly affected by the harvesting period from September 28 to October 20. 16. The tensile and bending detachment forces decreased with decrease of the moisture content from 23 to 13 percent (w.b.) by the natural drying, and the decreasing rate of detachment forces along the moisture content was the greater in the bending detachment force than the tensile detachment force.

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Influence of Different Supplements on the Commercial Cultivation of Milky White Mushroom

  • Alam, Nuhu;Amin, Ruhul;Khair, Abul;Lee, Tae-Soo
    • Mycobiology
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    • v.38 no.3
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    • pp.184-188
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    • 2010
  • Calocybe indica, known as milky white mushroom, grows and cultivated in the sub-tropical and temperate zones of South Asia. We investigated the most suitable supplements and their levels for the commercial cultivation of milky white mushroom. Rice bran, maize powder, and wheat bran with their different levels (10, 20, 30, 40, and 50%) were used as supplements to evaluate the yield and yield contributing characteristics of C. indica. Primordia initiation was observed between 13.5 and 19.3 days. The results indicated that the 30% maize powder supplement was effective for producing viable fruiting bodies. The maximum diameters of the pileus and stalk were observed with 30% maize powder. The highest biological and economic yield and biological efficiency were also obtained with 30% maize powder as a supplement. The results indicate that increasing the supplement level resulted in less biological efficiency, and that 30% maize powder was the best supplement level for rice straw substrate to cultivate milky white mushrooms.

Influence of the Application of Plant Growth-Regulating Hormone upon the Resistance to Grain Dropping of Indica-Japonica Species Rice (식물성장조절호르몬의 시용이 Indica-Japonica계 벼의 탈입저항에 미치는 영향)

  • 차균도;조성찬
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.26 no.4
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    • pp.66-71
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    • 1984
  • In order to find out the effect of a plant growth regulating hormone, commonly called as 2, 4, 5-TP, for checking the resisting energy against falling of grain of Indica-Japonica species rice, an experiment was conducted with five levels of consistency (5,10, 15, 20, 25 ppm) and with four levels of spray timing (8, 17, 27, 34 DAH). Ten applications were made for measuring the resisting energy of rice heads sampled from every lot of treatments. Followings are the results; 1.The rice heads applied with 2, 4, 5-TP showed remarkably higher resisting energy against falling of grain comparing with those from the control plot. 2.The highest average value of the resisting energy was found as 83, 326 erg per grain on the plots of 8 DAH spray. 3.The general tendency was found that the more the timing of spraying was early, the more the consistency was lean. 4.A regression equation for expressing the peak resistance versus the timing was derived by means of least squares as follow; C=0. 00042D$^3$-0. 012D$^2$+0. 267D+8. 394 5.No significant differences were found among the values of weight per 1, 000 grains neither among moisture content.

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