• Title/Summary/Keyword: Incubation temperature

Search Result 838, Processing Time 0.035 seconds

A Study on the Standardization of the Preparation Method for Waxy Rice Nochi (찹쌀 노치 제조법에 관한 연구)

  • 정영선;이혜수
    • Korean journal of food and cookery science
    • /
    • v.7 no.4
    • /
    • pp.9-13
    • /
    • 1991
  • Nochi is a kind of Korean traditional food made from glutinous rice or millet. This study attempted to examine the effects o( the various factors and to clarify the factors which affect the overall eating quality of Noch. The results were summarized as follows. 1. Starch content of waxy rice was 72.4% and IBC was 0.017%. 2. $\alpha$-amylase and $\beta$-amylase activity of malt were 40.13 and 8.94 units respectively. 3. Waxy rice soaked for 3 hours was ground to flour then sifted 20 mesh sieve. The mixture of waxy rice and malt was steamed for 20 minutes. 4. In sensory evaluation on varying the amount of malt and incubation time, the most favorite tendancy of the overall eating quality was at the level of 8hours incubation made by 5% addition of malt. 5. Total sugar contents, reducing sugar contents and the intensities of iodine stain at the incubation temperature of $60^{\circ}C$ were significantly different from those at $50^{\circ}C$.

  • PDF

Optimum Conditions for Glycoside Conversion to Aglycone by ${\gamma}-Galactosidase$ (유당분해효소에 의한 Glycoside의 Aglycone으로 전환을 위한 최적 조건 확립)

  • Kim, Nam-Chul;Jeon, Byung-Ju;Ahn, Joung-Jwa;Kwak, Hae-Soo
    • Food Science of Animal Resources
    • /
    • v.27 no.3
    • /
    • pp.329-336
    • /
    • 2007
  • This study was designed to find the optimum conditions for converting isoflavone glycoside to aglycone by ${\beta}-galactosidase$. Three different forms of the enzyme were tested and the optimum enzyme concentration, incubation temperature, pH, and incubation time were determined. Before treatment with enzyme, isoflavone contained 89.4% glycoside including daidzin, glycitin and genistin, and only 10.6% aglycone including daidzein, glycitein and genistein. Among the enzymes tested, the highest rate of isoflavone hydrolysis to aglycone, 35%, was observed when 3 unit/g Fungal Lactase (Amano Enzyme) was used. Higher incubation temperatures resulted in a higher rate of hydrolysis along with a greater loss of isoflavone mass. Therefore, body temperature $(37^{\circ}C)$ may be adequate for isoflavone conversion, with 44.9% hydrolysis and less than 10% loss of mass. As expected, a higher amount of aglycone was produced at pH 7 compared with other pH values. During 5hr of incubation, the conversion of glycoside to aglycone increased dramatically from 0 to 1hr, and plateaued thereafter. In addition, commercial soy-based milk was hydrolyzed more effectively with ${\beta}-galactosidase$ when incubated for 5hr. Based on the above results, the optimum conditions for isoflavone hydrolysis by ${\beta}-galactosidase$ were for 3 hr at $37^{\circ}C$, pH 7 with 3 unit/g Fungal Lactase (Amano Lactase), yielding an average total amount of aglycone ranging from 40 to 47%.

The Study of Nanocrystalline Silicon Bottom-gate Thin Film Transistor Fabricated at Low Temperature for Flexible Display

  • Lee, Youn-Jin;Lee, Kyoung-Min;Hwang, Jae-Dam;No, Kil-Sun;Yoon, Kap-Soo;Yang, Sung-Hoon;Hong, Wan-Shick
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2009.10a
    • /
    • pp.557-559
    • /
    • 2009
  • We attempted modulation of hydrogen dilution ratio to achieve both the minimal incubation layer and high deposition rate. The incubation layer thickness was estimated by transmission electron microscopy (TEM) and crystallization fraction was measured by Raman spectroscopy.

  • PDF

Studies on the Quality Control of Insulin Radioimnunoassay Kit (I) -Pitfalls in Radioimmunoassay of Insulin-

  • Lee, Kyung-Hee;Awh, Ok-Doo;Kim, Jae-Rok
    • Nuclear Engineering and Technology
    • /
    • v.10 no.4
    • /
    • pp.223-229
    • /
    • 1978
  • A typical abnormal standard dose response curve of nearly convex form. often encountered in insulin radioimmunoassay (IRIA) has been analyzed by varying incubation conditions. The cause of such abnormality has been turned out to be the results of an incomplete equilibrium between the two reactants. By careful control of the temperature of serum sample an immediate cause of sharp deviation of B/F value of tile sample tube from the measurable range in the standard curve has also been investigated. The two main troubles have been proven to be stemed from incubation conditions. Incubation at 4$^{\circ}C$ for 48 hrs is emphasized for IRIA.

  • PDF

Study on Motility and Acrosome Morphology of Fresh and Deep-frozen Korean Native Goat Spermatozoa (액상 및 동결보존된 한국재래산양 정자의 운동성 및 첨체형태에 관한 연구)

  • 황덕수;양문한;이규승;박창식
    • Korean Journal of Animal Reproduction
    • /
    • v.13 no.1
    • /
    • pp.18-25
    • /
    • 1989
  • This study was carried out to investigate the general semen characteristics of the Korean native goat and the effect of temperature, incubation time, dilution rate, freezing rate and glycerol concentration on motility and NAR (normal apical ridge) acrosome of fresh and frozen Korean native goat spermatozoa. 1. Average semen volume per ejaculate, motility, concentration and pH of fresh Korean native goat spermatozoa were 0.19${\pm}$0.09 ml, 94.5${\pm}$0.47%, 26.17${\times}$108${\pm}$1.68/ml and 6.63${\pm}$0.18, respectively. 2. Motility and NAR acrosome of fresh spermatozoa during incubation were higher at 22$^{\circ}C$ than at 5$^{\circ}C$ or 37$^{\circ}C$(P<.01). 3. Motility and NAR acrosome of spermatozoa diluted 1:4 during incubation were higher at 22$^{\circ}C$ than at 5$^{\circ}C$ or 37$^{\circ}C$(P<.01). 4. Motility and NAR acrosome of spermatozoa during incubation were higher for samples diluted 1:1, 1:2, or 1:4 than for samples diluted 1:6(P<.01). 5. Motility and NAR acrosome of post-thaw spermatozoa were higher at freezing rate of 12$^{\circ}C$/min than at freezing rate of 1$^{\circ}C$/min or 24$^{\circ}C$/min when glycerol concentration was 9%(P<.01).

  • PDF

Nucleation and Growth Rate of CVD-W on TiN (TiN상에서의 CVD-W의 핵생상 및 성장속도)

  • Kim, Eui-Song;Lee, Chong-Mu;Lee, Jong-Gil
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
    • /
    • 1992.11a
    • /
    • pp.28-30
    • /
    • 1992
  • Long incubation period of W nucleation on the TiN glue layer is a serious problem in blanket W process. In this study we investigated the dependence of W nucleation and growth rate on the preparation method of the TiN film, deposition temperature, chemistry, $SiH_4/WF_6$ ratio and sputter etching, ion implantation, and $SiH_4$ flushing pre-treatments. Incubation periods of W nucleation and deposition rates of W growth on three different TiNs are in the order of TiN>RTP-TiN> annealed TiN and TiN${\leq}$RTP-TiN${\leq}$ annealed TiN, respectively. $\beta$-W is not found on TiN substrate even for high $SiH_4/WF_6$ ratio. Sputter etching pre-treatment increases incubation period of W nucleation, while it decreases deposition rate. $SiH_4$ flushing pre-treatment decreases incubation period, but it slightly decreases deposition rate.

  • PDF

AN EXPERIMENTAL STUDY ON THE CYTOTOXICITY OF SEVERAL COMPOSITE RESINS (수종(數種) 치과(齒科) 수복용(修復用) 복합(複合)레진의 세포독성(細胞毒性)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Myoung, Jae-Keun;Lee, Myoung-Jong
    • Restorative Dentistry and Endodontics
    • /
    • v.14 no.1
    • /
    • pp.25-40
    • /
    • 1989
  • This study was conducted to evaluate the influence of 5 microfilled composite resin to fibroblast cultivated from human pulp (age 13). Each composite resin was manually mixed and filled in cylinder. Resin filled cylinders were placed in dishes (35mm in diameter) containing 3 ml of ${\alpha}$-MEM. Filters (pore size 0.22 ${\mu}m$) to simulate dentin were also placed between the bottom of cylinder and the dish floor. Then stored in 5% $CO_2$ containing incubator for 1 and 2 weeks at the temperature 36.6 C. The results analysed after 1 and 2 weeks were as follows: 1. Experimental group except group 2, 2 weeks incubation cases showed the cytotoxicity compared to the control group in cell count. 2. After 2 week-incubation of group 1 and group 4, cell count was more decreased than 1 week cases and cytotoxicity seemed to be constantly influenced to the cell multiplication. 3. The cell growth rate of 1 week incubation in group 3 and group 5 was similar to the control group and recognized the cytotoxicities of these groups were mild. 4. The cell multiplication rate of 2 week incubation cases in group 2 was greater than control group.

  • PDF

Production of the Microbial Chitosan from Rhizopus oligosporus ATCC22959 (Rhizopus oligosporus ATCC22959 균체에 의한 Chitosan의 생산)

  • 이윤동;이현수
    • The Korean Journal of Food And Nutrition
    • /
    • v.10 no.4
    • /
    • pp.491-496
    • /
    • 1997
  • To increase the productivity of microbial chitosan from Rhizopus oligosporus ATCC22959, production medium and incubation conditions were optimized. The composition of the medium and the incubation conditions were like follows : starch+glucose(1:1) 3.0%, yeast extract 3.0%, KH2PO4 0.05%, MgSO4 0.01%, ZnSO4 0.002%, pH 5.0, incubation temperature 3$0^{\circ}C$, and incubation time 96hours. The productivity of chitosan of optimized medium was about 6.4 times higher than that of basal medium.

  • PDF

A Study on the Hydriding Reaction of Pre-oxidized Zr Alloys (산화막을 입힌 지르코늄 합금의 수소화 반응에 관한 연구)

  • Kim, Sun-Ki;Bang, Je-Geon;Kim, Dae-Ho;Lim, Ik-Sung;Yang, Yong-Sik;Song, Kun-Woo
    • Journal of the Korean Ceramic Society
    • /
    • v.47 no.2
    • /
    • pp.106-112
    • /
    • 2010
  • This paper presents some experimental results on incubation time for massive hydriding of Zr alloys with oxide thickness. Oxide effects experiments on massive hydriding reaction of commercial Zr alloy claddings and pre-oxidized Zr alloys with hydrogen gas were carried out in the temperature range from 300 to $400^{\circ}C$ with thermo-gravimetric apparatus. Experimental results for oxide effects on massive hydriding kinetics show that incubation time is not proportional to oxide thickness and that the massive hydriding kinetics of pre-filmed Zr alloys follows linear kinetic law and the hydriding rate are similar to that of oxide-free Zr alloys once massive hydriding is initiated. There was a difference in micro-structures between oxide during incubation time and oxide after incubation time. Physical defects such as micro-cracks and pores were observed in only oxide after incubation time. Therefore, the massive hydriding of Zr alloys seems to be ascribed to short circuit path, mechacical or physical defects, such as micro-cracks and pores in the oxide rather than hydrogen diffusion through the oxide resulting from the increase of oxygen vacancies in the hypostoichiometric oxide.

Effects of NaCl/H3PO4 Flame Retardant Treatment on Lyocell Fiber for Thermal Stability and Anti-oxidation Properties (NaCl/H3PO4 내염화 처리가 라이오셀 섬유의 열 안정 및 내산화 특성에 미치는 영향)

  • Kim, Eun Ae;Bai, Byong Chol;Jeon, Young-Pyo;Lee, Chul Wee;Lee, Young-Seak;In, Se Jin;Im, Ji Sun
    • Applied Chemistry for Engineering
    • /
    • v.25 no.4
    • /
    • pp.418-424
    • /
    • 2014
  • The improved thermal stability and anti-oxidation properties of Lyocell fiber were studied based on flame retardant treatment by using NaCl/$H_3PO_4$ solution. The optimized conditions of flame retardant treatment were studied on various maxing ratio of NaCl and $H_3PO_4$ and the mechanism was proposed through experimental results of thermal stability anti-oxidation. The IPDT (integral procedural decomposition temperature), LOI (limited oxygen index) and $E_a$ (activation energy) increased 23, 30 and 24% respectively via flame retardant treatment. It is noted that thermal stability and anti-oxidation improved based on char and carbon layer formation by dehydrogenation and dissociation of C-C bond resulting the hindrance of oxygen and heat energy into polymer resin. The optimized conditions for efficient flame retardant property of Lyocell fiber were provided using NaCl/$H_3PO_4$ solution and the mechanism was also studied based on experimental results such as IDT (initial decomposition temperature), IPDT, LOI and $E_a$.