• 제목/요약/키워드: Incubation Temperature

검색결과 838건 처리시간 0.03초

찹쌀 노치 제조법에 관한 연구 (A Study on the Standardization of the Preparation Method for Waxy Rice Nochi)

  • 정영선;이혜수
    • 한국식품조리과학회지
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    • 제7권4호
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    • pp.9-13
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    • 1991
  • Nochi is a kind of Korean traditional food made from glutinous rice or millet. This study attempted to examine the effects o( the various factors and to clarify the factors which affect the overall eating quality of Noch. The results were summarized as follows. 1. Starch content of waxy rice was 72.4% and IBC was 0.017%. 2. $\alpha$-amylase and $\beta$-amylase activity of malt were 40.13 and 8.94 units respectively. 3. Waxy rice soaked for 3 hours was ground to flour then sifted 20 mesh sieve. The mixture of waxy rice and malt was steamed for 20 minutes. 4. In sensory evaluation on varying the amount of malt and incubation time, the most favorite tendancy of the overall eating quality was at the level of 8hours incubation made by 5% addition of malt. 5. Total sugar contents, reducing sugar contents and the intensities of iodine stain at the incubation temperature of $60^{\circ}C$ were significantly different from those at $50^{\circ}C$.

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유당분해효소에 의한 Glycoside의 Aglycone으로 전환을 위한 최적 조건 확립 (Optimum Conditions for Glycoside Conversion to Aglycone by ${\gamma}-Galactosidase$)

  • 김남철;전병주;안정좌;곽해수
    • 한국축산식품학회지
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    • 제27권3호
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    • pp.329-336
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    • 2007
  • This study was designed to find the optimum conditions for converting isoflavone glycoside to aglycone by ${\beta}-galactosidase$. Three different forms of the enzyme were tested and the optimum enzyme concentration, incubation temperature, pH, and incubation time were determined. Before treatment with enzyme, isoflavone contained 89.4% glycoside including daidzin, glycitin and genistin, and only 10.6% aglycone including daidzein, glycitein and genistein. Among the enzymes tested, the highest rate of isoflavone hydrolysis to aglycone, 35%, was observed when 3 unit/g Fungal Lactase (Amano Enzyme) was used. Higher incubation temperatures resulted in a higher rate of hydrolysis along with a greater loss of isoflavone mass. Therefore, body temperature $(37^{\circ}C)$ may be adequate for isoflavone conversion, with 44.9% hydrolysis and less than 10% loss of mass. As expected, a higher amount of aglycone was produced at pH 7 compared with other pH values. During 5hr of incubation, the conversion of glycoside to aglycone increased dramatically from 0 to 1hr, and plateaued thereafter. In addition, commercial soy-based milk was hydrolyzed more effectively with ${\beta}-galactosidase$ when incubated for 5hr. Based on the above results, the optimum conditions for isoflavone hydrolysis by ${\beta}-galactosidase$ were for 3 hr at $37^{\circ}C$, pH 7 with 3 unit/g Fungal Lactase (Amano Lactase), yielding an average total amount of aglycone ranging from 40 to 47%.

The Study of Nanocrystalline Silicon Bottom-gate Thin Film Transistor Fabricated at Low Temperature for Flexible Display

  • Lee, Youn-Jin;Lee, Kyoung-Min;Hwang, Jae-Dam;No, Kil-Sun;Yoon, Kap-Soo;Yang, Sung-Hoon;Hong, Wan-Shick
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2009년도 9th International Meeting on Information Display
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    • pp.557-559
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    • 2009
  • We attempted modulation of hydrogen dilution ratio to achieve both the minimal incubation layer and high deposition rate. The incubation layer thickness was estimated by transmission electron microscopy (TEM) and crystallization fraction was measured by Raman spectroscopy.

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Studies on the Quality Control of Insulin Radioimnunoassay Kit (I) -Pitfalls in Radioimmunoassay of Insulin-

  • Lee, Kyung-Hee;Awh, Ok-Doo;Kim, Jae-Rok
    • Nuclear Engineering and Technology
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    • 제10권4호
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    • pp.223-229
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    • 1978
  • 인슈린방사면역측정(IRIA)에서 흔히 목격하는 비정상적인 표준투여응답곡선 (standard dose response curve)을 정온유지 (incubation) 조건을 변화시킴으로써 해석 하였다. 그와 같은 이상(abnormality)의 원인은 두 반응물질간의 불안전한 평형에 있는 것으로 밝혀졌다. 인슈린측정용 혈청시료의 온도를 조절함으로써 혈청시료에 대한 B/F 값이 표준 곡선의 측정가능범위로부터 크게 벗어나는 원인을 조사할 수 있었다. 이들 두 실패요인은 주로 정온유지조건에 관계되었으므로 4$^{\circ}C$에서 48시간 실시하는 정온유지조건이 IRIA를 위하여 강조되었다.

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액상 및 동결보존된 한국재래산양 정자의 운동성 및 첨체형태에 관한 연구 (Study on Motility and Acrosome Morphology of Fresh and Deep-frozen Korean Native Goat Spermatozoa)

  • 황덕수;양문한;이규승;박창식
    • 한국가축번식학회지
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    • 제13권1호
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    • pp.18-25
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    • 1989
  • This study was carried out to investigate the general semen characteristics of the Korean native goat and the effect of temperature, incubation time, dilution rate, freezing rate and glycerol concentration on motility and NAR (normal apical ridge) acrosome of fresh and frozen Korean native goat spermatozoa. 1. Average semen volume per ejaculate, motility, concentration and pH of fresh Korean native goat spermatozoa were 0.19${\pm}$0.09 ml, 94.5${\pm}$0.47%, 26.17${\times}$108${\pm}$1.68/ml and 6.63${\pm}$0.18, respectively. 2. Motility and NAR acrosome of fresh spermatozoa during incubation were higher at 22$^{\circ}C$ than at 5$^{\circ}C$ or 37$^{\circ}C$(P<.01). 3. Motility and NAR acrosome of spermatozoa diluted 1:4 during incubation were higher at 22$^{\circ}C$ than at 5$^{\circ}C$ or 37$^{\circ}C$(P<.01). 4. Motility and NAR acrosome of spermatozoa during incubation were higher for samples diluted 1:1, 1:2, or 1:4 than for samples diluted 1:6(P<.01). 5. Motility and NAR acrosome of post-thaw spermatozoa were higher at freezing rate of 12$^{\circ}C$/min than at freezing rate of 1$^{\circ}C$/min or 24$^{\circ}C$/min when glycerol concentration was 9%(P<.01).

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TiN상에서의 CVD-W의 핵생상 및 성장속도 (Nucleation and Growth Rate of CVD-W on TiN)

  • 김의송;이종무;이종길
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 1992년도 추계학술대회 논문집
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    • pp.28-30
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    • 1992
  • Long incubation period of W nucleation on the TiN glue layer is a serious problem in blanket W process. In this study we investigated the dependence of W nucleation and growth rate on the preparation method of the TiN film, deposition temperature, chemistry, $SiH_4/WF_6$ ratio and sputter etching, ion implantation, and $SiH_4$ flushing pre-treatments. Incubation periods of W nucleation and deposition rates of W growth on three different TiNs are in the order of TiN>RTP-TiN> annealed TiN and TiN${\leq}$RTP-TiN${\leq}$ annealed TiN, respectively. $\beta$-W is not found on TiN substrate even for high $SiH_4/WF_6$ ratio. Sputter etching pre-treatment increases incubation period of W nucleation, while it decreases deposition rate. $SiH_4$ flushing pre-treatment decreases incubation period, but it slightly decreases deposition rate.

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수종(數種) 치과(齒科) 수복용(修復用) 복합(複合)레진의 세포독성(細胞毒性)에 관(關)한 실험적(實驗的) 연구(硏究) (AN EXPERIMENTAL STUDY ON THE CYTOTOXICITY OF SEVERAL COMPOSITE RESINS)

  • 명제근;이명종
    • Restorative Dentistry and Endodontics
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    • 제14권1호
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    • pp.25-40
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    • 1989
  • This study was conducted to evaluate the influence of 5 microfilled composite resin to fibroblast cultivated from human pulp (age 13). Each composite resin was manually mixed and filled in cylinder. Resin filled cylinders were placed in dishes (35mm in diameter) containing 3 ml of ${\alpha}$-MEM. Filters (pore size 0.22 ${\mu}m$) to simulate dentin were also placed between the bottom of cylinder and the dish floor. Then stored in 5% $CO_2$ containing incubator for 1 and 2 weeks at the temperature 36.6 C. The results analysed after 1 and 2 weeks were as follows: 1. Experimental group except group 2, 2 weeks incubation cases showed the cytotoxicity compared to the control group in cell count. 2. After 2 week-incubation of group 1 and group 4, cell count was more decreased than 1 week cases and cytotoxicity seemed to be constantly influenced to the cell multiplication. 3. The cell growth rate of 1 week incubation in group 3 and group 5 was similar to the control group and recognized the cytotoxicities of these groups were mild. 4. The cell multiplication rate of 2 week incubation cases in group 2 was greater than control group.

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Rhizopus oligosporus ATCC22959 균체에 의한 Chitosan의 생산 (Production of the Microbial Chitosan from Rhizopus oligosporus ATCC22959)

  • 이윤동;이현수
    • 한국식품영양학회지
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    • 제10권4호
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    • pp.491-496
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    • 1997
  • Rhizopus japonicus ATCC33959로부터 미생물성 chitosan을 생산하기 위한 최적 조건을 조사하였다. 탄소원으로써 starch와 glucose(1:1)를 3.0%, 질소원으로써 yeast extract를 3.0%, 무기염류로써 ZnSO4를 0.02% 첨가하고 pH를 5.0으로 맞춘 배지에 Rhizopus japonicus ATCC22959의 포자를 접종한 후 3$0^{\circ}C$에서 96시간 배양하는 것이었다. Chitosan 생산을 위한 기본 배지와 최적 배지에 각각 1.25$\times$105개의 포자를 접종하여 배양한 후 chitosan의 생산성을 비교한 결과는 각각 0.08g/200ml와 0.51g/200ml로 약 6.4배정도 수율이 증가하였다.

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산화막을 입힌 지르코늄 합금의 수소화 반응에 관한 연구 (A Study on the Hydriding Reaction of Pre-oxidized Zr Alloys)

  • 김선기;방제건;김대호;임익성;양용식;송근우
    • 한국세라믹학회지
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    • 제47권2호
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    • pp.106-112
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    • 2010
  • This paper presents some experimental results on incubation time for massive hydriding of Zr alloys with oxide thickness. Oxide effects experiments on massive hydriding reaction of commercial Zr alloy claddings and pre-oxidized Zr alloys with hydrogen gas were carried out in the temperature range from 300 to $400^{\circ}C$ with thermo-gravimetric apparatus. Experimental results for oxide effects on massive hydriding kinetics show that incubation time is not proportional to oxide thickness and that the massive hydriding kinetics of pre-filmed Zr alloys follows linear kinetic law and the hydriding rate are similar to that of oxide-free Zr alloys once massive hydriding is initiated. There was a difference in micro-structures between oxide during incubation time and oxide after incubation time. Physical defects such as micro-cracks and pores were observed in only oxide after incubation time. Therefore, the massive hydriding of Zr alloys seems to be ascribed to short circuit path, mechacical or physical defects, such as micro-cracks and pores in the oxide rather than hydrogen diffusion through the oxide resulting from the increase of oxygen vacancies in the hypostoichiometric oxide.

NaCl/H3PO4 내염화 처리가 라이오셀 섬유의 열 안정 및 내산화 특성에 미치는 영향 (Effects of NaCl/H3PO4 Flame Retardant Treatment on Lyocell Fiber for Thermal Stability and Anti-oxidation Properties)

  • 김은애;배병철;이철위;전영표;이영석;인세진;임지선
    • 공업화학
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    • 제25권4호
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    • pp.418-424
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    • 2014
  • 본 연구에서는 NaCl/$H_3PO_4$ 혼합수용액을 사용하여 라이오셀 섬유의 내염화 처리를 수행하고 이에 따른 열 안정성과 내산화성의 향상 효과를 고찰하였다. 라이오셀 섬유를 다양한 공정조건으로 내염화 처리한 후 열 안정성과 내산화성을 측정 및 분석하고 그에 따른 메커니즘을 제시하였다. 실험결과, 내염화 처리된 라이오셀 섬유의 적분 열분해 온도(integral procedural decomposition temperature, IPDT)와 한계산소지수(limited oxygen index, LOI)는 약 23, 30% 증가하였으며, 활성화 에너지(activation energy, $E_a$) 값은 약 24% 향상된 것을 알 수 있었다. 이러한 결과는 $H_3PO_4$와 NaCl가 연소시 에스테르화 반응, 탈수소화 반응 및 C-C결합의 분해반응으로 char 형성을 촉진하고 섬유 표면에 형성된 탄소 층을 형성함으로써, 고분자 수지 내부로 산소와 열 공급을 물리적으로 차단하여 열 안정성과 내산화성이 향상된 것으로 판단된다. 이러한 결과를 바탕으로, NaCl/$H_3PO_4$ 혼합수용액을 이용한 내염화 처리 공정의 최적화된 인자 및 메커니즘을 제시하였고 열 안정성과 내산화성이 향상된 라이오셀을 성공적으로 제조하였다.