• Industrial Engineering and Management Systems
• /
• 제13권3호
• /
• pp.330-341
• /
• 2014

An instrument which practitioners have acknowledged as an essential mechanism used in supporting greenhorn companies is the business incubation system. Several countries of the world have implemented the business incubation concept ever since it was conceived and developed in the United States in 1959. It is a model that has typically given itself much more readily within industrialized countries with identical environments to the United States. Nigeria also adapted it in 1993 where the resulting process and practice failed to live up to anticipations. To determine the significant role of government policy on the incubation dimensions and its success is the purpose of this research. Data collection process involved the surveying of stakeholders in Nigeria with some direct involvement in the national programme. The Partial Least Squares (PLS) was employed for the analysis. The findings showed that all exogenous variables collectively explained 52.4% of the variance in success. Meanwhile, when a moderation effect is present; the variable increased to 62.3%. Consequently, it is recommended that for a business incubation scheme to be successful and effective, government needs to implement efficient policies since results showed that these policies influenced all the relationships. The results' implications as well as limitations of the study are discussed.

• Journal of Electrochemical Science and Technology
• /
• 제3권1호
• /
• pp.24-28
• /
• 2012

The electrocatalytic activities and surface roughness of indium-tin-oxide (ITO) electrodes have been investigated after thermal treatment at 100, 150, or $200^{\circ}C$ for 30 min, 2 h, or 8 h. To check electrocatalytic activities, the electrochemical behavior of four electroactive species (p-hydroquinone, $Ru(NH_3){_6}^{3+}$, ferrocenemethanol, and $Fe(CN){_6}^{4-}$) has been measured. The electron transfer rate for p-hydroquinone oxidation and ferrocenemethanol oxidation increases with increasing the incubation temperature and the incubation period of time, but the rate for $Ru(NH_3){_6}^{3+}$ is similar irrespective of the incubation temperature and period because $Ru(NH_3){_6}^{3+}$ undergoes a fast outer-sphere reaction. Overall, the electrocatalytic activities of ITO electrodes increase with increasing the incubation temperature and period. The surface roughness of ITO electrodes increases with increasing the incubation temperature, and the thermal treatment generates many towering pillars as high as several tens of nanometer.

• 대한본초학회지
• /
• 제26권1호
• /
• pp.53-58
• /
• 2011

Objectives : The purpose of this study is to investigate effects of Scutellariae Radix Water Extract on hydrogen peroxide production in RAW 264.7 mouse macrophages. Methods : Scutellariae Radix produced from South Korea (SK) and Scutellariae Radix produced from China (SC) were extracted by hot water. Effects of SK and SC on hydrogen peroxide production in RAW 264.7 were measured by dihydrorhodamine 123 assay after 2, 4, 20, 24, 28, 44, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL. Results : SK significantly increase hydrogen peroxide production in RAW 264.7 cells for 2, 4, 20, 24, 28, 44, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). SC also significantly increase hydrogen peroxide production in RAW 264.7 cells for 4, 20, 24, 28, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). For 2 h incubation, SC significantly increase hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, and 100 ug/mL (P < 0.05). For 44 h incubation, SC significantly increase hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, and 50 ug/mL (P < 0.05). Conclusions : These results suggest that Scutellariae Radix has the immune - enhancing property related with its increasement of hydrogen peroxide production in macrophages.

• Toxicological Research
• /
• 제11권2호
• /
• pp.329-335
• /
• 1995

Incubation of aflatoxin $B_1$ $(AFB_1)$ with microsomes isolated from human liver number 110 yielded two metabolite peaks which were aflatoxin $Q_1$ $(AFQ_1)$ and $(AFB_1)$-exo-8, 9-epoxide (exo-epoxide) in high performance liquid chromatography. Production ratio of $AFQ_1$ to exo-epoxide was 2.43$\pm $0.04. Metabolism of $(AFB_1)$ to $(AFQ_1)$ and exo-epoxide was inhibited by troleandomycin in a same degree although troleandomycin was not activated as a mechanism-based inhibitor. The inhibitory effect was dependent upon either the incubation time with $(AFB_1)$ or the preincubation time before the addition of $(AFB_1)$. Incubation of troleandomycin and NADPH by the microsomes resulted in the formation of a cytochrome P 450 (P450)-metabollc intermediate (MI) complex and the level was approximately 80% of total P450 3A4 in the microsomes. This figure was similar to that of the inhibitory effect of troleandomycin on $AFB_1$ metabolism. Glutathione which was reported that it prevented the formation of MI complex in rat liver microsomes did not inhibit the formation of MI complex in human liver microsomes. These results suggested that the inhibitory effect of troleandomycin on $AFB_1$ metabolism is due to the formation of MI complex with P450 3A4. And the reaction mechanism of troleandomycin by human liver microsomes might be dlfferent from that one by rat liver microsomes.

• Asian-Australasian Journal of Animal Sciences
• /
• 제14권4호
• /
• pp.501-506
• /
• 2001

This experiment was conducted to evaluate the effects of supplemental unsaturated fatty acids (UFA) on fermentation characteristics, especially on gas production, cellulose degradation and volatile fatty acid (VFA) concentration by mixed ruminal microorganisms. In order to attain this objective, unsaturated fatty acids including oleic acid (C 18:1), linoleic acid (C18:2) and arachidonic acid (C22:4) were added at varying level. Mixed ruminal microbes used in this experiment were obtained from the rumen of a cannulated Holstein cow. Medium pH values after 7 d incubation were significantly affected by type and level of unsaturated fatty acids (p<0.01). All of UFA inhibited total gas production, and especially treatment of arachidonic acid at the levels of 0.01% gave the lowest gas. production after 7 d incubation (p<0.01). Comparison of the population of protozoa revealed that UFA did not have any significant effect on the total protozoa number. The addition of UFA did not effect dry matter degradation. Volatile fatty acid (VFA) composition of the culture was influenced little by UFA, although the considerable amount of iso-type VFA were detected in UFA supplemented incubations. The ratio of acetic acids to propionic acids, however, was lower than control in all the treatments after 7 d incubation (p<0.01).

• Ecology and Resilient Infrastructure
• /
• 제5권1호
• /
• pp.11-18
• /
• 2018

Phosphorous remains as one of the most limiting nutrients to plant growth, second only to nitrogen. Research on use of biochar as a soil amendment for available phosphorus in temperate calcareous soils has limited studies compared with to tropical acidic soils. An incubation experiment to assess phosphorous availability in a biochar amended calcareous soil under inorganic (Fused superphosphate, FSP) and organic fertilizer (bone meal, BM) and respectively, at the dose of 40, 80 and $120mg\;P\;kg^{-1}$ was carried out. Soil was incubated at $25^{\circ}C$ for 70 days. Results show that the rate of increase in available P was proportional to the fertilizer application rate with or without biochar amendment. Biochar did not have a significant effect on soils amended with either fertilizeron the values of available P. However, time had a significant effect (p<0.001) on the amount of available P during the incubation period. Inorganic fertilizer treatments had recorded high amount of available P with time compared to organic fertilizer treatments. Organic fertilizer treatment sample were significantly not different from control and for most of the incubation time biochar acted as a soil conditioner. Further research is required to understand the holistic and long-term effect of biochar.

• 벤처혁신연구
• /
• 제6권3호
• /
• pp.1-20
• /
• 2023

본 연구는 액셀러레이터 보육프로그램을 경험한 스타트업 대표자를 대상으로, 액셀러레이터 보육프로그램(멘토링, 초기투자)와 창업자기효능감이 경쟁우위성과에 영향을 주는지 실증분석을 하였고, 이때 혁신역량의 매개효과도 함께 살펴보았다. 표본의 수집은 전국 액셀러레이터, 창조경제혁신센터 등이 보육한 스타트업 대표자 대상으로 수집한 334건의 표본을 Smart PLS 4.0 프로그램을 사용하여 가설을 검정하였으며, 분석 결과는 다음과 같다. 첫째, 멘토링은 경쟁우위성과에 유의한 영향을 미치지 않지만, 초기투자와 창업자기효능감은 경쟁우위성과에 정(+)의 영향을 미치는 것으로 나타났다. 둘째 멘토링, 초기투자, 창업자기효능감은 혁신역량에 정(+)의 영향을 미치는 것으로 나타났다. 셋째, 혁신역량은 경쟁우위성과에 정(+)의 영향을 미치는 것으로 나타났다. 넷째, 혁신역량은 멘토링, 초기투자, 창업자기효능감과 경쟁우위성과 간에 정(+)의 영향을 미치며, 매개효과가 있음을 확인하였다. 결과적으로 멘토링은 경쟁우위성과에 직접적으로 유의한 영향을 주지 않지만, 혁신역량을 매개로 하여 유의한 영향이 있음을 확인되었으며, 초기투자, 창업자기효능감, 혁신역량은 경쟁우위성과에 직접적으로 도움이 되는 것으로 나타났고, 혁신역량이 매개 작용으로 경쟁우위성과에 영향 관계가 있음을 확인하였다. 본 연구를 통하여 스타트업의 경쟁우위성과를 높이는 데 도움이 될 수 있는 방향을 제시할 수 있을 것으로 기대된다.

• 약학회지
• /
• 제32권2호
• /
• pp.137-140
• /
• 1988

To investigate polyacetylene compounds isolated from petroleum ether extract of panax ginseng effect on the macromolecule synthesis, lympoid lukemia L1210 cell was incubated with them at 4, 8, 12,16 hours. Panaxydol, panaxynol and panaxytriol as cytotoxic substances inhibited the synthesis of macromolecule such as DNA, RNA and protein. Panaxydol which had the most potent cytotoxicity among these three compounds showed the strongest inhibitory effect on DNA, RNA and protein synthesis. For DNA and RNA synthesis, panaxynol and panaxytriol decreased the rate of inhibition with the incubation time but panaxydol had a strongest inhibitory effect at 16 hour incubation time. Protein synthesis was markedly inhibited by all these polyacetylene compounds. It was obserbed that there is a relationship between cytotoxicities of polyacetylene compounds and the inhibition of macromolecule synthesis.

• BMB Reports
• /
• 제30권1호
• /
• pp.1-6
• /
• 1997

The ability of Hep-G2 cells to process $[^{125}I]LDL$ under basal conditions was investigated. The receptor-binding and internalization of $[^{125}I]LDL$ increased with the time of incubation in a saturable manner. After 4 h of incubation, 31.4 ng of $[^{125}I]LDL$ was cell bound. The cells rapidly internalized $[^{125}I]LDL$ via specific, receptor-mediated endocytosis. The amount of internalized $[^{125}I]LDL$ reached a maximun of 96.7 ng at 2 h of incubation and remained constant for the next 2 h. The rate of degradation of internalized $[^{125}I]LDL$ proceeded in a linear manner over the entire 4 h of incubation after an initial lag period. The effects of individial fatty acids (C18:0. C18:1, C18:2. and C18:3), differing in their degree of unsaturation. on the receptor-binding, internalization and degradation of $[^{125}I]LDL$ were also investigated. Inclusion of 1.0 mM of each fatty acid into the culture medium significantly increased $[^{125}I]LDL$ metabolism in Hep-G2 cells. Among the fatty acids tested, stearic acid had the least effect on the receptor-binding activity. There were no significant differences among the unsaturated fatty acids in LDL-receptor binding. The effect of individual fatty acids on the $[^{125}I]LDL$ uptake was similar to that of the receptor-binding. showing a significantly lower effect with stearic acid. The amount of degraded material of internalized $[^{125}I]LDL$ was the lowest with stearic acid when it was compared with unsaturated fatty acids.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권3호
• /
• pp.364-368
• /
• 2011

An experiment was conducted to examine the effects of Cordyceps militaris mycelia on microbial populations and fibrolytic enzyme activities in vitro. C. militaris mycelia was added to buffered rumen fluid with final concentrations of 0.00, 0.10, 0.15, 0.20, 0.25 and 0.30 g/L and incubation times were for 3, 6, 9, 12, 24, 36, 48 and 72 h. At all incubation times, the supplementation of C. militaris mycelia linearly increased the number of total viable and celluloytic bacteria; maximum responses were seen with 0.25 g/L supplementation of C. militaris mycelia. The addition of C. militaris mycelia above the level of 0.20 g/L significantly (p<0.01) increased the number of total and cellulolytic bacteria compared with the control. On the other hand, the response of fungal counts to the supplementation of C. militaris mycelia showed a linear decrease; the lowest response was seen with 0.30 g/L supplementation of C. militaris mycelia. It would seem that C. militaris mycelia possess a strong negative effect on rumen fungi since the lowest level of C. militaris mycelia supplementation markedly decreased fungal counts. Carboxylmethyl cellulase activities were linearly increased by the addition of C. militaris mycelia except at 3 and 9 h incubation times. At all incubation times, the supplementation of C. militaris mycelia linearly increased the activities of xylanase and avicelase. In conclusion, the supplementation of C. militaris mycelia to the culture of mixed rumen microorganisms showed a positive effect on cellulolytic bacteria and cellulolytic enzyme activities but a negative effect on fungi.