Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of Cordyceps militaris Mycelia on Fibrolytic Enzyme Activities and Microbial Populations In vitro

  • Yeo, Joon-Mo (Department of Animal Science, Korea National Agricultural College, RDA) ;
  • Lee, Shin-Ja (Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University) ;
  • Shin, Sung-Hwan (Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University) ;
  • Lee, Sung-Hoon (Gyeongsangnam-Do Livestock Veterinary Research Institute) ;
  • Ha, Jong-Kyu (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Wan-Young (Department of Animal Science, Korea National Agricultural College, RDA) ;
  • Lee, Sung-Sill (Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University)
  • Received : 2009.02.02
  • Accepted : 2010.12.07
  • Published : 2011.03.01
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Abstract

An experiment was conducted to examine the effects of Cordyceps militaris mycelia on microbial populations and fibrolytic enzyme activities in vitro. C. militaris mycelia was added to buffered rumen fluid with final concentrations of 0.00, 0.10, 0.15, 0.20, 0.25 and 0.30 g/L and incubation times were for 3, 6, 9, 12, 24, 36, 48 and 72 h. At all incubation times, the supplementation of C. militaris mycelia linearly increased the number of total viable and celluloytic bacteria; maximum responses were seen with 0.25 g/L supplementation of C. militaris mycelia. The addition of C. militaris mycelia above the level of 0.20 g/L significantly (p<0.01) increased the number of total and cellulolytic bacteria compared with the control. On the other hand, the response of fungal counts to the supplementation of C. militaris mycelia showed a linear decrease; the lowest response was seen with 0.30 g/L supplementation of C. militaris mycelia. It would seem that C. militaris mycelia possess a strong negative effect on rumen fungi since the lowest level of C. militaris mycelia supplementation markedly decreased fungal counts. Carboxylmethyl cellulase activities were linearly increased by the addition of C. militaris mycelia except at 3 and 9 h incubation times. At all incubation times, the supplementation of C. militaris mycelia linearly increased the activities of xylanase and avicelase. In conclusion, the supplementation of C. militaris mycelia to the culture of mixed rumen microorganisms showed a positive effect on cellulolytic bacteria and cellulolytic enzyme activities but a negative effect on fungi.

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References

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