• Title/Summary/Keyword: Incubating

Search Result 358, Processing Time 0.025 seconds

Characteristics and pathogenicity of the Entomopathogenic Fungus Beauveria bassiana101AA on the silkworm (Bombyx mori) (백강균(Beauveria bassiana)101A의 특성 및 가잠(Bombyx mori)에 대한 병원성 검정)

  • 정이연;남성희;조세연
    • Journal of Sericultural and Entomological Science
    • /
    • v.42 no.2
    • /
    • pp.99-103
    • /
    • 2000
  • This study was carried out to investigate incubating characteristics, LTSEM observation, nature of a disease of infected silkworms(bombyx mori), LD$\_$50/, optimum infective condition for mass production and infection percentage(%) on conservation periods. On the V8 media, the colony was 0.5∼2.0cm/8 days in diameter and white or slighty colored with a white fluffy to powdery appearance. The conidia was subglobos in shape, zig-zag appearance, 1-celled, hyaline and 3.2$\times$2.4 ㎛ in size on the average. The conidiophore was irregular grouped, hyaline, rounded or flask-shaped. The LD$\_$50/ values of the 2nd and 4th silkworms were each other 6.6142(Log) in natural temperature and humidity (25$\^{C}$, 65%). On optimum infective condition for mass production, all of preservation time(hr.) postinoculation in 1.0$\times$10$\^$8/ conidia/㎖ was over 97% and only 20 hr in 1.0$\times$10$\^$7/ condia/㎖ was over 90%.

  • PDF

Plant Regeneration from Mesophyll Protoplasts Culture of Solanum sisymbriifolium

  • Kim Hag-Hyun;Shin Un-Dong
    • Journal of Plant Biotechnology
    • /
    • v.7 no.3
    • /
    • pp.169-174
    • /
    • 2005
  • The optimal culture conditions were studied for plant regeneration from mesophyll protoplasts of Solanum sisymbriifolium. Axenic seedlings of S. sisymbriifolium were used as a explant for protoplast culture. Many viable protoplasts were isolated by incubating leaf slices in an enzyme solution containing 0.25% Meicerase and 0.05% Macerozyme for 16 hr at $25^{\circ}C$ without shaking. Protoplast density of $5.0{\times}10^4\;ml^{-1}$ in Kao medium containing 5.0 mg/L NAA, 1.0 mg/L 2,4-D and 1.0 mg/L BA was optimal for colony formation. Most colonies were formed when protoplasts were cultured at $25^{\circ}C$ after initial culture at $30^{\circ}C$ for one week. On the MS agar medium with 1.0 mg/L zeatin, 38.4% of protoplast-derived calli differentiated shoots. These shoots rooted on 1/2MS medium with 5.0 g/L sucrose and 2.5 g/L gellan gum, and developed into whole plants.

Inhibitory Effects of Methanol Extracts from Korean Medicinal Plants against HIV-1 Protease Activity

  • Park, Jong-Cheol;Miyashiro, Hirotsugu;Hattori, Masao
    • Korean Journal of Medicinal Crop Science
    • /
    • v.11 no.4
    • /
    • pp.264-267
    • /
    • 2003
  • Korean medicinal plants were screened for their inhibitory activity against HIV-1 protease. The inhibitory activity of protease was determined by incubating the extracts in reaction mixtures containing protease and substrate $His-Lys-Ala-Arg-Val-Leu-(p-NO_{2}-Phe)-Glu-Ala-Nle-Ser-NH_{2}$ to perform proteolytic cleavage reactions. In this study the twenty six extracts from medicinal plants were investigated. Of the extracts tested, the extracts from the stem of Morus alba. exhibited the strongest activity with inhibition of 81% at a concentration of $100{\mu}g/ml$. The extracts of the flower of Saxjfraga stolonifera, and stems of Euonymus japonica and Castanea crenata showed appreciable inhibitory activity (>50%) against HIV-1 protease at same concentration.

The Disruption Yeast Cell Wall by chemical Treatment (화학적 처리방법에 의한 효모의 세포벽 제거)

  • 문정혜;김중균
    • Journal of Life Science
    • /
    • v.8 no.2
    • /
    • pp.197-202
    • /
    • 1998
  • The cell of Kluyveromyces fragilis yeast, which is worthy of an algal substitute, was disrupted by a chemical treatment to increase the digestion of filter-feeders that yeasts are fed to. The optimum conditions of the chemical treatment were obtained by incubating yeasts at 3$0^{\circ}C$ for one hour after treated by 1 M of Na$_{2}$-EDTA that was dissolved in 0.2 M of Tris-buffer and by 0.3 m of 2-mercaptoethanol. The percentage of protop[last production was about 30%. The percentage could be doubled by the pretreatment of three times of 30 seconds sonication.

  • PDF

The Development of Venture Firms in Taejon, with Special Reference to Spin-offs from Taeduk Science Town (대전지역 벤처기업의 현황 및 활성화 방안 - 대덕연구단지에서 스핀오프된 벤처기업을 중심으로 -)

  • 민완기;신동호
    • Journal of Korea Technology Innovation Society
    • /
    • v.2 no.1
    • /
    • pp.58-73
    • /
    • 1999
  • The venture firms originating from the government-financed research institutes of Taeduk Science Town play a dominant role in Taejon. Those venture fm have the following characteristics. First, they are innovative in the growth stage, although not so innovative in the beginning stage. Second, they still largely depend on the domestic market because of their import-substitution strategy. Third, they mostly lack administrative capabilities. Finally, the infrastructure of venture firms is not good in spite of many supporting government policies. The efficient management of the existing business incubators, the establishment of special venture capital, and continuous supports by the incubating organizations should be warranted to encourage the spin-offs from the government- financed research institutes.

  • PDF

Effect of Gamma Irradiation on Egg White Protein (卵白단백질에 대한 감마선 조사의 영향)

  • 변명우;임성일;육홍선;윤혜현;김영지
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.27 no.2
    • /
    • pp.291-295
    • /
    • 1998
  • The ovalbumins obtained from hen's and duck's egg white were irradiated with up to 100 kGy at room temperature. The purified proteins were evaluated for their in vitro digestibility by incubating successively with pepsin and pancreatin conjugate. Amino acid compositions and SDS-PAGE pattern in these proteins were also analyzed. The obtained results indicated that gamma irradiation within the tested dose range(up to 100kGy) produced no statistically significant changes in the in vitro digestibility an amino acid compositions. Analysis of gamma-irradiated ovalbumins by SDS-PAGE revealed radiolysis of ovalbumin into proteins or peptides of low molecular weights.

  • PDF

Antioxidative Effect of Histidine and Alanine on Oil Rancidity (Histidine과 Alanine의 유지에 대한 항산화 효과)

  • 조희숙
    • Journal of the East Asian Society of Dietary Life
    • /
    • v.9 no.1
    • /
    • pp.93-99
    • /
    • 1999
  • The purpose of this study was to investigate the antioxidant and synergistic effects upon different concentrations(0.02, 0.1, l%) of histidine and alanine in soybean oil during incubation at 6$0^{\circ}C$. Acid value(AV), peroxide value (POV) and thiobarbituric acid(TBA) value of each oil was monitored. Histidine and alanine showed antioxidative effects in all concentrations. In the case of the incubating antioxidative effect of histidine was lower than that of TBHQ. That of alanine was considerably higher than that of $\alpha$-tocopherol, but was lower than that of histidine. Synergistic effects among histidine, alanine and some food antioxidants were shown to exist available in all substrates and the best effect was shown in substrate added compound of histidine and $\alpha$-tocopherol.

  • PDF

A study on the correlation between the objectives of being members and their request to activate the operation of Business Incubation Systems (창업보육센터 활성화를 위한 입주목적과 지원요구사항간의 상관관계에 관한 연구)

  • Park, Roh-Gook
    • Journal of Korean Society of Industrial and Systems Engineering
    • /
    • v.28 no.3
    • /
    • pp.109-114
    • /
    • 2005
  • The founding purpose of Business Incubation System is to induce those businessmen who have technology and promising business ideas but are lacking fund, information, and management skill, and to speed up their business establishment and enhance the level of success by providing them with managerial knowledge, production technology, design, and equipment. While some universities made Business Incubation Center to achieve the above purpose, some other universities, especially the local ones, believed that Business Incubation Center, if managed successfully, could be an accelerator picking up the sluggish local economies. This study studied the motivators that let them move in an Incubating Center, their request for support, their level of expert utilization, and the role of head of a Center. This study found a significant level of correlation among the variables such as industrial-educational cooperation, the development of common items, utilization of professors with matching majors, utilization of research professors, and transfer of technology.

Add to Cart or Buy It Now? Factors Influencing the Usage Intention of Online Shopping Cart

  • Kim, Namhee;Chun, Sungyong
    • Journal of Information Technology Applications and Management
    • /
    • v.25 no.2
    • /
    • pp.117-132
    • /
    • 2018
  • This study examines the reason why consumers try to use the online shopping cart more, and especially analyzes the influence of personal psychological factor such as regulatory focus. Hypotheses are tested with a two-way ANOVA model using experimental data collected from 210 undergraduate students at a business school in South Korea. We found that prevention focused consumers try to use online shopping cart more than promotion focused consumers. It is possible that prevention focused consumers try to consider as many alternatives as possible to ensure safety as they focus on negative results or losses during shopping online. However, we also found out when the buying purpose is utilitarian, promotion focused consumers are not different from the promotion in terms of the usage intention of online shopping carts. Marketing managers can provide different messages customized for their consumers by leading them to use the online shopping cart in a more effective way.

Commercialization of Bioinformatics: Importance of External Integration (바이오인포매틱스 제품의 상용화 : 외부통합의 중요성)

  • Soh, Hong-Seok;Choung, Jae-Yong
    • Journal of Technology Innovation
    • /
    • v.12 no.3
    • /
    • pp.229-258
    • /
    • 2004
  • To promote successful bioinformatics commercialization in terms of CoPS, we try to explore a commercialization process based on the characteristics of the product. Our study shows that external integration with sector-specific infrastructure is a critical factor to obtaining a knowledge base for CoPS commercialization, and that sector-specific local infrastructure is useful resource for early commercialization stages(imagining, incubating, and demonstrating stages). This paper analyzes Ensoltek's commercialization process on the basis of analysis of bioinformatics industry and Daeduck Science Park. This leads us to suggest the following policy implication for supporting commercialization activities in bioinformatics industry ; long-term investment policy towards infrastructure and software R&D, promotion policy for collaboration, and introduction of commercialisation based industry-university-public research institute cooperation.

  • PDF