• 한국수산과학회지
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• 제54권1호
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• pp.124-128
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• 2021

This study was conducted to evaluate the effects of incubation methods and transfer timings on the hatching rate of fertilized eggs of the river puffer Takifugu obscurus. Four incubation methods were tested, a) control (fertilized eggs attached to the glass plate), b) bottom (fertilized eggs spread on the bottom of the tank without any treatment), c) S-bottom (removing the stickiness of the fertilized eggs, and then spreading the eggs on the bottom of the tank), and d) incubator (removing the stickiness of the fertilized eggs, and then incubating the eggs in an incubator). Additionally, four transfer timings were tested: a) control (no transfer from the incubation tank), b) zygote (fertilized eggs transferred at the zygote stage), c) segmentation (fertilized eggs transferred at the segmentation stage), and d) pharygula (fertilized eggs transferred at the pharygula state). The results showed that the hatching rate of incubator was significantly higher than those of control, bottom, and glass (P<0.05). The results also showed that the hatching rates of control and pharygula were significantly higher than those of zygote and segmentation (P<0.05).

• Journal of Species Research
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• 제11권3호
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• pp.143-154
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• 2022

To obtain unrecorded bacterial species in Korea, various marine samples were collected from Jeollanam-do Province, Korea in 2021. After plating the samples on marine agar and marine R2A agar, and incubating aerobically and anaerobically, approximately 1200 bacterial strains were isolated and identified using 16S rRNA gene sequences. A total of 30 strains showed ≥98.7% 16S rRNA gene sequence similarity with validly published bacterial species but not reported in Korea, indicating that they are unrecorded bacterial species in Korea. The unrecorded bacterial strains belonged to 4 phyla, 7 classes, 13 orders, 19 families, and 22 genera, which were assigned to Azospirllium, Loktanella, and Pseudovibrio of the class Alphaproteobacteria; Grimontia, Halomonas, Marinobacter, Microbulbifer, Photobacterium, Pseudoalteromonas, Pseudidiomarina, Ferrimonas, Shewanella, Simiduia, Thalassotalea, and Vibrio of the class Gammaproteobacteria; Priestia and Enterococcus of the class Bacilli; Persicobacter of the class Cytophagia; Aureivirga of the class Flavobacteriia; Propionigenium and Psychrilyobacter of the class Fusobacteriia; and Tepidibacter of the class Clostridia. The details of the unreported species including Gram reaction, colony and cell morphology, biochemical characteristics, and phylogenetic position are also provided in the description of the strains.

• Journal of Species Research
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• 제12권spc2호
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• pp.54-59
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• 2023

To obtain unrecorded bacterial species from Korean islands, various samples were collected from the islands in 2022. After plating the samples on marine agar or Reasoner's 2A, and incubating aerobically, approximately 1,200 bacterial strains were isolated and identified using 16S rRNA gene sequences. A total of 10 strains showed ≥98.7% 16S rRNA gene sequence similarity with the bacterial species that were validly published but not reported in Korea. The unrecorded bacterial strains belong to three phyla, five classes, 10 orders, 10 families, and 10 genera, which are assigned to Sphingomonas, Falsirhodobacter and Asticcacaulis of the class Alphaproteobacteria; Colwellia and Halomonas of the class Gammaproteobacteria; Chitinophaga of the class Chitinophagia; Chryseobacterium of the class Flavobacteriia; Microlunatus, Zhihengliuella, and Streptomyces of the class Actinomycetia. The details of the unreported species including Gram reaction, colony and cell morphology, biochemical characteristics, and phylogenetic position are also provided in the description of the strains.

• Journal of Species Research
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• 제12권4호
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• pp.267-276
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• 2023

To obtain unrecorded bacterial species in Korea, various soils of coastal areas were collected from the Republic of Korea in 2022. After plating the samples on marine agar and incubating aerobically and anaerobically, approximately 1,700 bacterial strains were isolated and identified using 16S rRNA gene sequences. A total of 20 strains showed ≥98.7% 16S rRNA gene sequence similarity with validly published bacterial species but not reported in Korea, indicating they are unrecorded bacterial species in Korea. The unrecorded bacterial strains belonged to four phyla, six classes, 15 orders, 16 families, and 19 genera which were assigned to Blastomonas and Sphingomonas of the class Alphaproteobacteria; Pseudidiomarina, Kushneria, Salinicola, and Salinisphaera of the class Gammaproteobacteria; Evansella, Virgibacillus, and Paenibacillus of the class Bacilli; Cyclobacterium of the class Cytophagia; Pedobacter of the class Sphingobacteriia; and Demequina, Ornithinimicrobium, Blastococcus, Jatrophihabitans, Kineococcus, Glaciihabitans, Aeromicrobium and Streptomyces of the class Actinomycetes. The details of the 20 unreported species, including Gram reaction, morphology, biochemical characteristics, and phylogenetic position are also provided in the description of the strains.

• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1189-1196
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• 2023

This study proposed to demonstrate the neuroprotective effects of heat-killed Levilactobacillus brevis KU15152. Heat-killed L. brevis KU15152 showed antioxidant activity similar to that of Lacticaseibacillus rhamnosus GG, in terms of radical scavenging activity. To evaluate the neuroprotective effects, conditioned medium (CM) obtained by incubating heat-killed bacteria in intestinal cells (HT-29) was used through gut-brain axis. CM from L. brevis KU15152 protected neuroblastoma cells (SH-SY5Y) against H₂O₂-induced oxidative stress. Pretreatment with CM significantly alleviated the morphological changes induced by H₂O₂. Heat-killed L. brevis KU15152 showed an increased brain-derived neurotrophic factor (BDNF) expression in HT-29 cells. L. brevis KU15152-CM remarkably downregulated the Bax/Bcl-2 ratio, while upregulating the expression of BDNF and tyrosine hydroxylase (TH) in SH-SY5Y cells. Furthermore, L. brevis KU15152-CM reduced caspase-3 activity following H₂O₂ treatment. In conclusion, L. brevis KU15152 can be potentially used as food materials to avoid neurodegenerative diseases.

• 한국IT서비스학회지
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• 제23권3호
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• pp.91-101
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• 2024

Based on the analysis of 174 Young Startups Project Papers from university students majoring in entrepreneurship, this study aimed to identify the thoughts and characteristics of prospective entrepreneurs and derive their main agendas. Detailed information was collected on their preparation and ambitions for entrepreneurship, market research in their fields of interest, analysis of potential target users, requirements, and team management. We refined, categorized, and interpreted 84,818 words across 396 digital pages on LMS, and visualized using word clouds program. Additionally, interviews about peer learning were conducted by Zoom. The analysis revealed unique differences in students' perspectives on entrepreneurship based on their major, academic year, and gender. Also we could find that they actively utilize AI tech with a strong spirit of challenge in the startups. Interestingly, they emphasized horizontal communication and collaboration capabilities to improve productivity. This suggests the need to consider diversity in the design of entrepreneurship incubating content and programs for generation Z technically and academically.

• Journal of Species Research
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• 제13권3호
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• pp.293-305
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• 2024

Various samples were collected from Korean islands in order to obtain unrecorded bacterial species in 2023. After aerobically incubating on marine agar and Reasoner's 2A agar, approximately 1,200 bacterial strains were isolated and identified using 16S rRNA gene sequences. A total of 36 strains showed ≥98.7% sequence similarity to previously published and validated bacterial species. However, these strains have not previously been reported in the Republic of Korea, indicating that they belong to Korean unrecorded bacterial species. The unrecorded bacterial species were assigned to the classes Actinomycetes, Bacilli, Bacteroidia, Flavobacteriia, Sphingobacteriia, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria. The information we obtained by examining the strains includes details of the Gram reactions, colony and cell morphology, biochemical characteristics, and phylogenetic positions of the unrecorded species.

• Asian Pacific Journal of Cancer Prevention
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• 제13권1호
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• pp.289-294
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• 2012

Background: Myofibroblasts play an important role in the development of oral submucous fibrosis (OSF). In the current study, we investigate the effect of curcumin on growth and apoptosis of myofibroblasts derived from human oral mucosa. Methods: Myofibroblasts were generated by incubating fibroblasts, obtained from human oral mucosa, with transforming growth factor-${\beta}1$ (TGF-${\beta}1$). MTT, PI staining, and FACS assays were used to investigate curcumin's effect on proliferation and cell cycle of fibroblasts and myofibroblasts. Annexin V/PI binding and FACS assays were used to examine apoptosis of myofibroblasts, Western blotting to determine the levels of Bcl-2 and Bax, and enzyme-linked immunosorbant assay was employed to examine the levels of collagen type I and III in the supernatants of myofibroblasts. Results: Curcumin inhibits proliferation of fibroblasts and myofibroblasts; it also disturbs the cell cycle, induces apoptosis and decreases the generation of collagen type I and III in myofibroblasts, which are more sensitive to its effects than fibroblasts. Curcumin induces apoptosis in myofibroblasts by down-regulating the Bcl-2/ Bax ratio. Conclusion: Our results demonstrate the antifibrotic effect of curcumin in vitro. It may therefore be a candidate for the treatment of OSF.

• 벤처창업연구
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• 제11권2호
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• pp.135-144
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• 2016

중소기업청은 대학 및 연구기관의 인적(교수, 연구원) 물적(설비, 공간) 자원을 활용하기 위하여 1998년부터 최근까지 약 4,000여억원의 예산을 투입하여 창업보육센터(Business Incubator, BI)의 건립 및 운영을 지원하여 왔다. 그 결과 2013년 말 기준으로 5,500여개의 기업이 보육공간에 입주하여 매출 1조 6천억원과 고용인력 1만 6천여명을 성과를 창출하였다. 하지만 이러한 양적인 성장에도 불구하고 하드웨어 중심의 지원, 기관 내 자원의 활용부족, 민간부문과의 협력부족, 스타기업 발굴 부진 등의 여러 문제점이 노출되었다. 본 연구는 이러한 질적 성장의 한계점에 봉착한 창업보육센터의 역량강화 및 혁신방안을 모색하기 위하여 창의성 개념을 도입하여 센터의 개선방향을 제시하고자 한다. 특히 죽음의 계곡을 극복하는 방법으로서 기술사업화의 관점에서 역량, 제품, 시장이라는 CPM(Capability, Product, Market) 조합이 원활히 될 수 있도록 보육센터의 역할을 제시하였다. 또한 대학 내 자원의 유기적 연계를 제안하였다.

• 유기물자원화
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• 제8권3호
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• pp.104-111
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• 2000

본 연구는 제지슬러지퇴비의 농업적 이용을 위하여 퇴비를 사질양토에 일정 비율로 혼합한 후 인산의 가용화와 토양중의 무기 인산의 변화를 구명하기 위하여 수행하였다. 온도와 수분에 관한 영향을 찾고자 incubation 실험을 실시하였다. Incubation 실험의 처리내용은 제지슬러지퇴비의 혼합율을 0, 2, 8및 16%의 비율로 혼합하였고, 수분조건은10과 15%로 하였으며, 온도는 4와 $30^{\circ}C$로 하였다. Incubation 실험에서 제지슬러지퇴비의 혼합비율이 증가할수록 pH값은 상승하였으며, 처리조건중 온도조건에서는 뚜렷한 경향이 없었고, 수분의 조건에서 pH변화는 없었다. Incubation 실험에서 무기인산의 분획물중 Saloid-P와 Fe-P는 혼합량이 증가할수록 감소하였으며, 처리량이 많았던 8과 16% 처리구에서는 존재하지 않거나 미량 존재 하였다. 시간에 따른 변화량은 낮게 나타났다. Al-P에서는 혼합량이 증가할수록 증가하는 경향이었다. Ca-P의 함량은 혼합량이 증가할수록 증가하는 경향이었다.