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http://dx.doi.org/10.5657/KFAS.2021.0124

Effects of Incubation Method and Transfer Timing on the Hatching Rate of Fertilized Eggs of the River Puffer Takifugu obscurus  

Yoo, Gwangyeol (Food & Biological Resources Examination Division, Korean Intellectual Property Office)
Hur, Junwook (Faculty of Marine Applied Biosciences, Kunsan National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.54, no.1, 2021 , pp. 124-128 More about this Journal
Abstract
This study was conducted to evaluate the effects of incubation methods and transfer timings on the hatching rate of fertilized eggs of the river puffer Takifugu obscurus. Four incubation methods were tested, a) control (fertilized eggs attached to the glass plate), b) bottom (fertilized eggs spread on the bottom of the tank without any treatment), c) S-bottom (removing the stickiness of the fertilized eggs, and then spreading the eggs on the bottom of the tank), and d) incubator (removing the stickiness of the fertilized eggs, and then incubating the eggs in an incubator). Additionally, four transfer timings were tested: a) control (no transfer from the incubation tank), b) zygote (fertilized eggs transferred at the zygote stage), c) segmentation (fertilized eggs transferred at the segmentation stage), and d) pharygula (fertilized eggs transferred at the pharygula state). The results showed that the hatching rate of incubator was significantly higher than those of control, bottom, and glass (P<0.05). The results also showed that the hatching rates of control and pharygula were significantly higher than those of zygote and segmentation (P<0.05).
Keywords
Puffer; Hatching rate; Incubation method; Transfer timing; Adhesiveness;
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