• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2005.11a
• /
• pp.252-252
• /
• 2005

• 한국약용작물학회:학술대회논문집
• /
• 2008.05a
• /
• pp.170-171
• /
• 2008

• Proceedings of the Korean Society of Crop Science Conference
• /
• 1996.10a
• /
• pp.108-109
• /
• 1996

• Clinical and Experimental Pediatrics
• /
• v.46 no.7
• /
• pp.718-721
• /
• 2003

The isoimmune hemolytic disease of newborn due to the incompatibility of minor blood groups is characterized by progressive neonatal hyperbilirubinemia and anemia caused by the IgG antibody transmitted from the mother to the fetus. Recently we had a case of hemolytic disease in a newborn due to $anti-Jk^b$. There were no ABO and Rh(D) incompatibilities between mother and baby. The infant's direct and indirect antiglobulin tests were strongly positive. From the mother and baby, an irregular antibody was found and identified as $anti-Jk^b$. Generally, hemolytic disease of the newborn resulting from $anti-Jk^b$ incompatibility has a benign clinical course and a good prognosis. This patient completely recovered without exchange transfusion. We report this case with a brief review of relevant literature.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.8
• /
• pp.1163-1178
• /
• 2010

Plasmids play important roles in horizontal gene transfer among Vibrionaceae, but surprisingly little is known about their replication and incompatibility systems. In this study, we successfully developed a bioinformatics-assisted strategy of experimental identification of seven Vibrio plasmid replicons. Comparative sequences analysis of the seven Vibrio plasmid replicons obtained in this study together with eight published Vibrionaceae plasmid sequences revealed replication-participating elements involved in the ColE1 mode of replication initiation and regulation. Like plasmid ColE1, these Vibrionaceae plasmids encode two RNA species (the primer RNA and the antisense RNA) for replication initiation and regulation, and as a result, the 15 Vibrionaceae plasmids were designated as ColE1-like Vibrionaceae (CLV) plasmids. Two subgroups were obtained for the 15 CLV plasmids, based on comparison of replicon organization and phylogenetic analysis of replication regions. Coexistence of CLV plasmids were demonstrated by direct sequencing analysis and Southern hybridization, strongly suggesting that the incompatibility of CLV plasmids is determined mainly by the RNA I species like the ColE1-like plasmids. Sequences resembling the conserved Xer recombination sites were also identified on the CLV plasmids, indicating that the CLV plasmids probably use the host site-specific recombination system for multimer resolution like that used by ColE1-like plasmids. All the results indicated that the 15 plasmids form a new ColE1-like group, providing a basis for the rapid characterization and classification of Vibrionaceae plasmids.

• Journal of Forest and Environmental Science
• /
• v.33 no.3
• /
• pp.197-201
• /
• 2017

Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at ${\alpha}=0.01%$. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at ${\alpha}=0.05%$. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.

• Korea Journal of Hospital Management
• /
• v.23 no.2
• /
• pp.95-105
• /
• 2018

Objective: This study conducted an empirical research on foreign patients who had visited the medical institutions of Daegu region in order to identify the effects of the factor satisfaction and experience satisfaction with medical services on their revisit intentions. Methods: This study reviewed the existing studies on medical services to foreign patients with a main focus on medical tourism, and conducted the empirical research through a survey on foreign patients from the Russian, Chinese and English-speaking regions based on the Daegu Medical Tourism Promotion Institute's DB of foreign patients. The empirical research conducted a multiple regression analysis on factor satisfaction and experience satisfaction regarding return visit intentions. Results: The multiple regression analysis results showed that both the factor satisfaction and the experience satisfaction with medical services of foreign patients had positive effects on their return visit intentions, especially, with a relatively greater effect from the factor satisfaction. Specifically, it was found that factor satisfaction elements of 'friendliness of employees', 'good communication', and 'expertise of medical workers'; and experience satisfaction elements of 'reliability of medical information', 'efforts to minimize incompatibility towards foreigners', 'convenient access to medical information' and the like enhanced the return visit intentions. Conclusion: We discovered that, in the context of medical tourism activities, it is important to enhance factor satisfaction and experience satisfaction with medical services in order to promote return visits of the foreign patients who we once attracted. Especially, there are implications that reliability of medical information and efforts to minimize incompatibility towards foreigners enhance return visits of foreign patients.

• Journal of Plant Biotechnology
• /
• v.42 no.3
• /
• pp.271-276
• /
• 2015

Crab apple cultivars, 'Maypole', 'Tuscan', and 'Manchurian', were evaluated as potential pollinizers of major apple cultivars, 'Fuji', 'Hongro', and 'Tsugaru', cultivated in high-density apple orchard systems. Numerous cultivar characteristics, including blooming time, pollen germination, fruit set, disease and pest resistance, and self-incompatibility, were examined. The blooming times of both 'Maypole' and 'Tuscan' ranged from April 19 to May 5, which was 2-4 days earlier than those of the major commercial apple cultivars. PCR analysis did not reveal the presence of any of the S-alleles (S1, S3, S7, or S9) identified in major commercial apple cultivars. In addition, the percentage of the fruit set was high after trees were artificially fertilized with crab apple pollen. Artificially cross-pollinated fruits were of similar or higher quality than open-pollinated fruits. They also demonstrated resistance to apple blotch, sooty blotch, and fly speck. The results indicate that the two crab apples, 'Maypole' and 'Tuscan' would be potential candidates for pollinizers of major apple cultivars in Korea. Use of the pollen of these crap apples in commercial production will improve fruit quality and promote sustainable and robust fruit production.

• The Korean Journal of Mycology
• /
• v.36 no.2
• /
• pp.144-147
• /
• 2008

Inonotus obliquus could be isolated from Betula platyphylla var. japonica with diameter in the range of 6$\sim$13 cm that artificially inoculated by the fungus. The diameter and/or inoculation point of tree did not show any significant relationships with the infection rate of the fungus. Inonotus obliquus showed rapid growth on vertical direction of the infected tree while the growth was quite low on radial direction. The isolated fungus from the infected tree did not show vegetative incompatibility with the original fungus used for inoculation. We could isolate 8 contaminants from the inoculated area; Trichoderma reesei, T. atroviride, Cryptococcus neoformans, Botrytis cinerea, Fusarium sp. and 3 unknown species.

• Korean journal of applied entomology
• /
• v.40 no.4
• /
• pp.321-326
• /
• 2001

Contribution of Wolbachia infection to fitness of a species (developmental time, adult life span, fecundity and ovipositional period) was measured in the susceptible and dicofol-resistant strains of two spotted spider mite, Tetranychus urticae Koch, on miniature roses. Based on ftsZPCR assay, Wolbachia infection was confirmed only in the susceptible strain. The susceptible strain had significantly higher fecundity (eggs/female) and shorter developmental times than the resistant strain. Longer adult life span and ovipositional period were observed in the susceptible strain. Fitness differences were appeared to influence dicofol resistance development. Similar measurements were performed with progeny from two reciprocal $F_1$crosses. Similar to other examples of cytoplasmic incompatibility induced by Wolbachia, subsequent cross between uninfected female and infected male spider mites were different from the other combination: a high egg mortality and a male-biased sex ratio. When the intrinsic rate of natural increase was calculated, the cross between uninfected female and infected male spider mites had a significantly lower rate($0.09\pm$0.01) than did the other combination ($0.20\pm$0.01). These results suggest that the dynamic and evolution of the fitness are closely associated with dicofol resistance and Wolbachia infection in the two spotted spider mites.