The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Artificial Inoculation of Inonotus obliquus on Betula platyphylla var. japonica

자작나무를 이용한 차가버섯균의 인공접종

  • Lee, Bong-Hun (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Park, Hyun (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Lee, Hye-Min (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Bak, Won-Chull (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Ryu, Sung-Ryul (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute)
  • 이봉훈 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과) ;
  • 박현 (국립산림과학원 화학미생물과) ;
  • 이혜민 (국립산림과학원 화학미생물과) ;
  • 박원철 (국립산림과학원 화학미생물과) ;
  • 유성열 (국립산림과학원 화학미생물과)
  • Published : 2008.12.31
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Abstract

Inonotus obliquus could be isolated from Betula platyphylla var. japonica with diameter in the range of 6$\sim$13 cm that artificially inoculated by the fungus. The diameter and/or inoculation point of tree did not show any significant relationships with the infection rate of the fungus. Inonotus obliquus showed rapid growth on vertical direction of the infected tree while the growth was quite low on radial direction. The isolated fungus from the infected tree did not show vegetative incompatibility with the original fungus used for inoculation. We could isolate 8 contaminants from the inoculated area; Trichoderma reesei, T. atroviride, Cryptococcus neoformans, Botrytis cinerea, Fusarium sp. and 3 unknown species.

차가버섯균을 접종한 자작나무를 조사한 결과, 6$\sim$13cm 직경의 나무들에서 균이 확인되었다. 또한 나무 직경과 차가버섯균의 활착 간에는 유의성이 없는 것으로 나타났으며 접종 위치에 따른 활착 차이도 거의 없었다. 차가버섯균이 활착된 나무의 절단면 관찰에서는 차가버섯균이 위아래 방향으로는 활발히 움직인 반면에 방사상으로는 그렇지 않음을 알 수 있었다. 그리고 나무에서 분리한 균과 보관균 간의 대치배양에서는 대치선이 형성되지 않았다. 차가버섯 접종목에서 8종(Trichoderma reesei, T. atroviride, Cryptococcus neoformans, Botrytis cinerea, Fusarium sp., 명확하게 되지 않는 균 3종)의 오염균이 분리되었다.

Keywords

References

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