• Journal of Oral Medicine and Pain
• /
• v.32 no.1
• /
• pp.35-43
• /
• 2007

This study was conducted to investigate the preventing effects of OPB (water extracts of Rehmannia glutinosa Libosch and Eleutherococcus senticosus Max) on bone loss in ovariectomized rats. Twenty Sprague Dawley rats of 13 week-old were divided into two groups: control group (ovariectomized, OVX)) and experimental group (OVX + OPB). The preventing effects of OPB on bone loss, OPB were fed with 100 mg OPB/kg body weight from 3 days after ovariectomization. The duration of the treatment period was 8 weeks. All bone mineral density, bone mineral content indices and bone strength indices measured by peripheral quantitative computerized tomography (pQCT) and serum bone marker assessment were carried out at end of experiment. pQCT scanning showed that OVX induced a significant decrease in cancellous bone mineral density in the proximal tibia ($-29.8{\pm}3.0%$). These decreases were significantly prevented by the administration of OPB 100 mg/kg ($-21.4{\pm}2.3%$. p<0.05). Bone strength indices showed significant difference between OVX and OPB treated rats (anti-fracture, anti-twisting, p<0.05). These data suggested that administration of OPB inhibited the loss of bone in OVX rats. CTx level were lower than in the OPB-treated animals compared with OVX. However there was no significant difference between OVX and OPB treated OVX rat. Our results suggest that OPB is effective in preventing the development of bone loss induced by ovariectomy in rats.

• Journal of Microbiology
• /
• v.40 no.3
• /
• pp.211-218
• /
• 2002

A 4.8-kb DNA fragment encoding the P-450 type hydroxylase and ferredoxin genes was cloned from Pseudonocardia autotrophica IFO 12743 that can convert vitamin D$\_$3/ into its hydroxylated active forms. In order to isolate the P-450 gene cluster in this organism, we designed PCR primers on the basis of the regions of an oxygen binding site and a heme ligand pocket that are general characteristics of the P-450 hydroxylase. Sequencing analysis of the BamHI fragment revealed the presence of four complete and one incomplete ORFs, named PauA, PauB, PauC, and PauD, respectively. As a result of computer-based analyses, PauA and PauB have homology with enoyl-CoA hydratase from several organisms and the positive regulators belonging to the tetR family, respectively. PauC and PauD show similarity with SuaB/C proteins and ferredoxins, respectively, which are composed of P-450 monooxygenase systems for metabolizing two sulfonylurea herbicides in Streptomyces griseolus PauC shows the highest similarity with another CytP-450$\_$Sca2/ protein that is responsible for production of a specific HMG-CoA reductase inhibitor, pravastatin, in S. carbophilus. Cultures of Steptomyces lividans transformant, containing the P-450 gene cluster on the pWHM3 plasmid, was unable to convert vitamin D$\_$3/ to its hydroxylated forms.

• Proceedings of the SAREK Conference
• /
• 2008.06a
• /
• pp.552-557
• /
• 2008

This paper introduces to schematic design for Korea Institute of Toxicology Second Campus at Jeong-eup and provides fundamental information and data in order to design this laboratory facility to meet the criteria of G.L.P(Good Laboratory Practice), AAALAC(Association for Assessment and Accreditation of Laboratory Animal Care) and so on. This paper especially focus on design criteria, HVAC(Heating Ventilating and Air Conditioning) equipment and systems, plumbing equipment and systems, maintenance of equipment and systems and energy saving system of this laboratory facility.

• Proceedings of the Korea Information Processing Society Conference
• /
• 2011.04a
• /
• pp.953-955
• /
• 2011

본 제품은 RFC(Recursive Flow Classification) 알고리듬의 네트워크 접근제어를 구현한 방화벽 기반의 정보유출방지 솔루션이다. 네트워크 단에서의 정보유출은 대부분 이메일, 메신저, 웹하드, P2P 등을 통해 이루어진다. 따라서 본 제품은 업로드 트래픽의 크기를 제한하고 사용자가 송수신하는 모든 메일을 필터링 하여 저장한다. 웹상에서는 정보유출 가능성이 있는 URL을 등록, 사용을 제한하는 기능을 통하여 네트워크를 통한 정보유출의 가능성을 원천적으로 차단한다. 동시에 사용자 중심의 인터페이스와 성능이 뛰어나면서도 저렴한 통합 플랫폼을 제공함으로써 중소기업환경에 최적화된 네트워크 정보보안의 대안을 제시한다.

• Journal of Korean Society of Disaster and Security
• /
• v.14 no.3
• /
• pp.51-63
• /
• 2021

This study is for the quality improvement of water-sealing & reinforcement grouting in tunnel of the construction of the underground transportation network underneath the city. Existing tunnel grouting process did not technically utilize P~q~t charts fully. It is due to the absence of technical methods to decide how P~q~t charts change in the presence of trouble and what the change represents in grouting. There were no standards to decide which chart pattern represents which ground characteristics, how to categorize ground types, and how to take measures according to the standards. This paper studies on the grouting type, ground characteristics, ground type categorizing method, and countermeasures for both general and algorithm-processed grouting in soil and rock layer to address the aforementioned problems. Newly improved P~q~t charts from grouting in soil was categorized into six different types. Different characteristics and categorization method was developed for each type. Countermeasures for each type of grouting process were developed so that on-site application can be readily available. Improved P~q~t charts for rock layer also have six different types of grouting. Each type was given the countermeasures for rock layer grouting process for easier applications. Therefore, it is expected to be used through out the entire process of grouting from preparation to the last report of the water-sealing & reinforcement grouting in tunnel of the construction of the underground transportation network underneath the city.

• The monthly packaging world
• /
• s.156
• /
• pp.115-128
• /
• 2006

• The monthly packaging world
• /
• s.51
• /
• pp.118-121
• /
• 1997

• Korean Journal of Microbiology
• /
• v.25 no.2
• /
• pp.87-93
• /
• 1987

The properties of the plasmid pKU41 isolated from Pseudomonas putkda KU190 have been investigated, pKU41 was defined as an R plasmid having a transmissible ampicillin and tetracycline resistance determinant, and could be classified as a plasmid belonging to IncP-1 group according to incompatibility grouping.

• Journal of Sensor Science and Technology
• /
• v.25 no.1
• /
• pp.51-56
• /
• 2016

This study evaluates the performance of an optical sensor and measurement system (CMA-24) which can analyze the fluctuation of dissolved oxygen and pH simultaneously. In the optical sensor system, the fluorescent materials, Rudpp and HPTS which are sensitive to dissolved oxygen and pH, respectively, are coated on the bottom of a 24-well -plate by the sol-gel technology. The detection times of the emission light of the oxygen sensor were $4,186{\pm}13.90{\mu}s$ and $4,452{\pm}36.68{\mu}s$ for the dissolved oxygen of 17% $O_2$ and 7.6% $O_2$, respectively. On the other hand, the detection times of the pH sensor were $6,699.43{\pm}14.64{\mu}s$, $6,722.24{\pm}6.21{\mu}s$, and $6,748.52{\pm}2.63{\mu}s$ using pH 6, 7, and 8, respectively. When we determined cellular respiration levels of C2C12 myocytes with CMA-24, $O_2$/pH measurement system, the ratio of the uncoupled to coupled OCR (oxygen consumption rate) was 1.41. The results mean that this CMA-24 system shows almost the same sensitiveness as the commercial system.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.5
• /
• pp.705-709
• /
• 2021

Porphyromonas gingivalis (P. gingivalis) is a major bacterial pathogen that causes periodontitis, a chronic inflammatory disease of tissues around the teeth. Periodontitis is known to be related to other diseases, such as oral cancer, Alzheimer's disease, and rheumatism. Thus, a precise and sensitive test to detect P. gingivalis is necessary for the early diagnosis of periodontitis. The objective of this study was to optimize a rapid visual detection system for P. gingivalis. First, we performed a visual membrane immunoassay using 3,3',5,5'-tetramethylbenzidine (TMB; blue) and coating and detection antibodies that could bind to the host laboratory strain, ATCC 33277. Antibodies against the P. gingivalis surface adhesion molecules RgpB (arginine proteinase) and Kgp (lysine proteinase) were determined to be the most specific coating and detection antibodies, respectively. Using these two selected antibodies, the streptavidin-horseradish peroxidase (HRP) reaction was performed using a nitrocellulose membrane and visualized with a detection range of 10³-10⁵ bacterial cells/ml following incubation for 15 min. These selected conditions were applied to test other oral bacteria, and the results showed that P. gingivalis could be detected without cross-reactivity to other bacteria, including Streptococcus mutans and Escherichia fergusonii. Furthermore, three clinical strains of P. gingivalis, KCOM 2880, KCOM 2803, and KCOM 3190, were also recognized using this optimized enzyme immunoassay (EIA) system. To conclude, we established optimized conditions for P. gingivalis detection with specificity, accuracy, and sensitivity. These results could be utilized to manufacture economical and rapid detection kits for P. gingivalis.