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http://dx.doi.org/10.5369/JSST.2016.25.1.51

Characteristics and Fabrication of Dissolved Oxygen and pH Measurement System based on the Optical Sensor for Analysis of Cell Metabolic Functions  

Jang, Jiwoon (Technology Development Laboratory, DKInnotech Inc.)
Hwang, Insook (Technology Development Laboratory, DKInnotech Inc.)
Lee, Jongmok (Technology Development Laboratory, DKInnotech Inc.)
Lee, Sunmin (Division of Gastroenterology, Seoul National University Bundang Hospital)
Kang, Sora (Department of Neurocscience, Graduate School, Kyung Hee University)
Kim Pak, Youngmi (Department of Neurocscience, Graduate School, Kyung Hee University)
Kim, Nayoung (Division of Gastroenterology, Seoul National University Bundang Hospital)
Publication Information
Journal of Sensor Science and Technology / v.25, no.1, 2016 , pp. 51-56 More about this Journal
Abstract
This study evaluates the performance of an optical sensor and measurement system (CMA-24) which can analyze the fluctuation of dissolved oxygen and pH simultaneously. In the optical sensor system, the fluorescent materials, Rudpp and HPTS which are sensitive to dissolved oxygen and pH, respectively, are coated on the bottom of a 24-well -plate by the sol-gel technology. The detection times of the emission light of the oxygen sensor were $4,186{\pm}13.90{\mu}s$ and $4,452{\pm}36.68{\mu}s$ for the dissolved oxygen of 17% $O_2$ and 7.6% $O_2$, respectively. On the other hand, the detection times of the pH sensor were $6,699.43{\pm}14.64{\mu}s$, $6,722.24{\pm}6.21{\mu}s$, and $6,748.52{\pm}2.63{\mu}s$ using pH 6, 7, and 8, respectively. When we determined cellular respiration levels of C2C12 myocytes with CMA-24, $O_2$/pH measurement system, the ratio of the uncoupled to coupled OCR (oxygen consumption rate) was 1.41. The results mean that this CMA-24 system shows almost the same sensitiveness as the commercial system.
Keywords
Fluorescent sensing membrane; Dissolved oxygen; pH; Oxygen consumption rate; cellular respiration level;
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  • Reference
1 A. Niazi and C. J. Anthony, "Development of oxygen sensor by integrating the low cost printed circuit board technology and solid electrolyte membrane", Proc. Of ICBES, p.137, Prague, Czech, 2014.
2 E. Gnaiger, Drug-Induced Mitochondiral Dysfunction, John Wiley & Sons, New York, pp. 327-352, 2008.
3 H. K. Lee, Y. M. Cho, Y. Y. Lee, and Y. K. Pak, "Mitochondria-based model for fetal origin of adult disease and insulin resistance", Ann. N. Y. Acad Sci., Vol. 1042, pp. 1-18, 2005.   DOI
4 M. L. Hitchman, Measurement of dissolved oxygen, Wiley, New York, p. 255, 1978.
5 J. Lin and D. Liu, "An optical pH sensor with a linear response over a broad range", Anal. Chem Acta., Vol. 408, pp. 49-55, 2000.   DOI
6 J. Goicoechea, C. R. Zamarreno, I. R. Matias, and F. J. Arregui, "Optical fiber pH Sensor based on layer-by-layer electrostatic self-assembled neutral red", Sens. Actuators B, Vol. 132, pp. 305-311, 2008.   DOI
7 C. Mcdoagh, C. Kolle, A. K. Mcevoy, D. L. Dowling, A. A. Cafolla, S. J. Cullen, B. D. Maccraith, "Phase fluorometric dissolved oxygen sensor", Sens. Actuators B,, Vol. 74, pp. 124-127, 2001.   DOI
8 G. O'keeefe, B. D. Maccraith, A. K. Mcevoy, C. Mcdonagh, and J. F. Mcgilp, "Development of a led-based phase fluorimetric oxygen sensor using evanescent wave excitation of a sol-gel immobilized dye", Sens. Actuators, Vol. 29, pp. 226-230, 1995.   DOI
9 G. Holst, R. N. Glud, M. Kuhl, and I. Klimant, "A microoptode array for fine-scale measurement of oxygen distribution", Sens. Actuators, Vol. 29, pp. 123-129, 1997.
10 D. Wencel, C. Higgins, A. Klukowska, B. D. Maccraith, and C. Mcdonagh, "Novel sol-gel derived films for luminescence-based oxygen and pH sensing", Materials Science-Poland, Vol. 25, No. 3, pp. 768-770, 2007.
11 D. A. Nivens, Y. Zhang, and S. M. Angel, "A fiber-optic pH sensor prepared using a base-catalyzed organo-silica solgel", Anal. Chim. Acta., Vol. 376, pp. 235-245, 1998.   DOI
12 O. B. Miled, H. B. Ouada, and J. Livage, "pH sensor based on a detection sol-gel layer onto optical fiber", Mater. Sci. Eng. C, Vol. 21, pp. 183-188, 2002.   DOI