Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Characteristics and Fabrication of Dissolved Oxygen and pH Measurement System based on the Optical Sensor for Analysis of Cell Metabolic Functions

세포대사 기능 분석을 위한 광학센서 기반 용존산소와 pH 측정 시스템의 제작 및 특성 분석

  • Jang, Jiwoon (Technology Development Laboratory, DKInnotech Inc.) ;
  • Hwang, Insook (Technology Development Laboratory, DKInnotech Inc.) ;
  • Lee, Jongmok (Technology Development Laboratory, DKInnotech Inc.) ;
  • Lee, Sunmin (Division of Gastroenterology, Seoul National University Bundang Hospital) ;
  • Kang, Sora (Department of Neurocscience, Graduate School, Kyung Hee University) ;
  • Kim Pak, Youngmi (Department of Neurocscience, Graduate School, Kyung Hee University) ;
  • Kim, Nayoung (Division of Gastroenterology, Seoul National University Bundang Hospital)
  • 장지운 ((주)동국이노텍 기술개발연구소) ;
  • 황인숙 ((주)동국이노텍 기술개발연구소) ;
  • 이종목 ((주)동국이노텍 기술개발연구소) ;
  • 이선민 (분당 서울대학교 병원 소화기내과) ;
  • 강소라 (경희대학교 대학원 신경과학과) ;
  • 김영미 (경희대학교 대학원 신경과학과) ;
  • 김나영 (분당 서울대학교 병원 소화기내과)
  • Received : 2015.12.09
  • Accepted : 2016.01.25
  • Published : 2016.01.29
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Abstract

This study evaluates the performance of an optical sensor and measurement system (CMA-24) which can analyze the fluctuation of dissolved oxygen and pH simultaneously. In the optical sensor system, the fluorescent materials, Rudpp and HPTS which are sensitive to dissolved oxygen and pH, respectively, are coated on the bottom of a 24-well -plate by the sol-gel technology. The detection times of the emission light of the oxygen sensor were $4,186{\pm}13.90{\mu}s$ and $4,452{\pm}36.68{\mu}s$ for the dissolved oxygen of 17% $O_2$ and 7.6% $O_2$, respectively. On the other hand, the detection times of the pH sensor were $6,699.43{\pm}14.64{\mu}s$, $6,722.24{\pm}6.21{\mu}s$, and $6,748.52{\pm}2.63{\mu}s$ using pH 6, 7, and 8, respectively. When we determined cellular respiration levels of C2C12 myocytes with CMA-24, $O_2$/pH measurement system, the ratio of the uncoupled to coupled OCR (oxygen consumption rate) was 1.41. The results mean that this CMA-24 system shows almost the same sensitiveness as the commercial system.

Keywords

References

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