• Title/Summary/Keyword: Inbred lines

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A Study on the Improvement Methods for Hybrid Sorghum Seeds Production

  • Ji-Young Kim;Sang-ik Han;Seok-bo Song;Byeong-won Lee;Ji-ho Chu;Young-kwang Ju;Chung Song Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.205-205
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    • 2022
  • Sorghum (Sorghum bicolor L.) is a major cereal grain crop of the world and It can be cultivated under high temperature and dry condition with high adaptability to the adverse environment. It is usually eaten with rice in korea and most of domestic sorghum cultivars have been developed by pure line selection and cross breeding. In prior studies, Hybrid cultivar (F1 seed) was known to improve 30-40% in yield and resist to disease and pest than pure line varieties due to hybrid vigor. Therefore, study on the hybrid sorghum is continuously necessary. In korea, hybrid sorghum cultivar have been developed, so we need to find methods seed production technology for supply and commercialization of hybrid seed cultivar. Female inbred line(A-line) and male inbred line(R-line) are needed for hybrid seed production. This study was carried out to investigate growth characteristics and yield of hybrid sorghum according to the seedling period and planting rate between female inbred line(YSA1) and male inbred line (Sodamchal, Miryang 19). When the flowering period of two inbred groups in the process of hybrid seed production is coincides, hybrid seed production is increase. The female inbred line and male inbred lines were cultivated at Daegu in 2021. Two inbred lines were sown at intervals of 3 days and 7days and were evaluated flowering period. As a result, the flowering period of the female inbred line and male inbred lines were matched at the 7days interval. To find out optimal planting rate, two inbred lines were cultivated under different plating rate (4:2, 5:2, 6:2). Yield of YSA1/Sodamchal(F1) was the highest 130(kg/10a) in the 4:2 ratio and yield of YSA1/Miryang 19 was the highest quantity of 139(kg/10a) in the 6:2 ratio. The results of this study could be helpful for hybrid Sorghum seeds production.

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Chemical Composition of Seed from Inbred Lines and Hybrids of Maize Recently Developed in Korea (국내 육성 주요 옥수수 자식계통 및 교잡종의 종실 성분 특성)

  • Son, Beom-Young;Kim, Jung-Tae;Lee, Jin-Seok;Baek, Seong-Bum;Kim, Sun-Lim;Ku, Ja-Hwan;Hwang, Jong-Jin;Cha, Sun-Mi;Kwon, Young-Up
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.2
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    • pp.188-194
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    • 2012
  • This study was carried out to evaluate the quality properties, fatty acid composition and amino acid composition of major Korean inbred lines and hybrids of maize for corn breeding. Protein content of $F_2$ seeds was 9.0%, which was slightly lower than those of inbred lines, 11.4%, and $F_1$ seeds, 10.9%. in hybrid maizes. Lipid content of $F_2$ seeds of hybrid maizes was 4.2%, slightly higher than those of inbred lines, 3.3%, and $F_1$ seeds, 3.6%. However, there was no significant difference in ash content. The amount of linoleic acid in inbred lines, $F_1$ and $F_2$ seeds of hybrid maizes was highest among all fatty acids and followed by oleic acid, palmitic acid, stearic acid and linolenic acid. The composition of saturated fatty acid of $F_2$ seeds was 17.6%, similar to $F_1$ seeds but it was lower than that of inbred lines. The composition of unsaturated fatty acid of $F_2$ seeds was 82.4%, similar to $F_1$ seeds but was higher than inbred lines. The content of leucine of $F_2$ seeds was lower than inbred lines and $F_1$ seeds. The content of valine, serine, threonine, cysteine, aspartic acid, lysine, arginine, histidine of $F_2$ seeds was higher than inbred lines and $F_1$ seeds. MMA (monoamino monocarboxylic acid) in inbred lines, $F_1$ and $F_2$ seeds of hybrid maizes was highest among all amino acids and followed by DMA (diamino monocarboxylic acid) and AAA (aromatic amino acid).

Analysis of Morphological Characteristics Among Popcorn Inbred Lines (튀김옥수수 자식계통들에 대한 형태적 특성)

  • Chang, Eun-Ha;Sa, Kyu Jin;Kim, Jong-Hwa;Lee, Ju Kyong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.3
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    • pp.267-273
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    • 2013
  • We evaluated the morphological characteristics of 79 popcorn inbred lines, which were developed to breeding popcorn variety at Maize Experiment Station, Gangwon Agricultural Research and Extension Services, by examining ten quantitative and three qualitative characteristics. In the survey of 3 qualitative traits for 79 popcorn inbred lines, most inbred lines respectively showed purple (46 inbred lines) at tassel color (QL1), green (55inbred lines) at silk color (QL2) and green (75 inbred lines) at stem color (QL3). While, on the survey of 10 quantitative traits among 79 popcorn inbred lines, they showed the morphological variation in plant height (QN1, $174.2{\pm}34.9$ cm), ear height (QN2, $103.4{\pm}24.7$ cm), ear length (QN3, $9.4{\pm}3.0$ cm), kernel setting length (QN4, $8.4{\pm}2.6$ cm), ear thickness (QN5, $24.9{\pm}7.7$ mm), ear row number (QN6, $14.0{\pm}2.3$ number), ear weight (QN7, $36.5{\pm}26.0$ g), kernel weight (QN8, $30.9{\pm}19.3$ g), 100 kernel weight (QN9, $10.4{\pm}3.8$ g) and germination rate (QN10, $95.3{\pm}8.1%$), respectively. As a result, 5 inbred lines (PS0-001, PS0-003, PS1-002, PS1-003, PS2-009) in the 79 popcorn inbred lines have showed comparatively high values for the five quantitative traits. On the principal component analysis, silk color (QL2), ear length (QN3), kernel setting length (QN4), ear thickness (QN5), ear weight (QN7), kernel weight (QN8) and 100 kernel weight (QN9) greatly contributed in positive direction on the first principal components, whereas tassel color (QL1), stem color (QL3), ear height (QN2) and ear row number (QN6) contributed in negative direction on the first principal component. In addition, plant height (QN1), ear height (QN2), and kernel weight (QN8) contributed in positive direction on the second principal component, and also tassel color (QL1), silk color (QL2), ear length (QN3), ear row number (QN6) and 100 kernel weight (QN9) contributed in negative direction on the second principal component.

Block Designs for Comparisons within Two Groups of Inbred Lines in Diallel Crosses

  • Choi, Kuey-Chung;Lee, Jang-Jae;Oh, Ji-Ae
    • Proceedings of the Korean Statistical Society Conference
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    • 2002.05a
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    • pp.165-170
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    • 2002
  • Sometimes we have two groups of inbred lines and there are only interest in gca comparisons within group($p_{1}$) and group($p_{2}$) and not between two groups. For example, suppose there two Lab, each of the 2 Labs have obtained the best lines. For this purpose we now give a method of constructing block designs for diallel cross experiments and we will explain how to calculate efficiency. Then we show the efficiencies in the table.

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Morphometric Characterization of Honey Bee, Apis mellifera Linnaeus, Inbred Lines in Korea (국내 서양종꿀벌 순계의 형태적 특징)

  • Frunze, Olga;Сhoi, Yong Soo;Kim, Dong Won;Park, Bo Sun;Park, Hee Geun;Kang, Eun Jin
    • Korean journal of applied entomology
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    • v.59 no.4
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    • pp.371-382
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    • 2020
  • The A, C, F colonies of Apis mellifera ligustica Spin. and D, V colony of Apis mellifera caucasia Gorb. bees were collected from 2005-2007. Consequently, inbred lines were derived from the bees of original colonies by matting in the isolated island with due regard for pure breeding. This project helps in the selection of colonies with higher production capacity, aiming to improve honey and royal jelly production and breeding programs. Twenty-three standard morphological traits of honeybee were evaluated, and samples were compared with the data of the two original subspecies. The result suggested that 8 traits partly preserved in bees of inbred lines, and the bees from A. m. ligustica preserved more traits than bees from A. m. caucasia. Among the studied inbred lines, the F line is distinguished by an increase in leg parameters, considered as a favorable phenotypic trait of inbred lines. Importantly, bred of beelines in the same area can be classified as remote and isolated areas. Therefore, we observed differences of inbred lines with the origin subspecies in description acquired with morphometric characteristics as a result of adaptation, breeding, purebred individual lines used as an important resource for breeding novel cross-breeding colonies.

Block Designs For Comparisons Within Two Groups of Inbred Lines in Diallel Crosses

  • Son, Young-Nam
    • Communications for Statistical Applications and Methods
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    • v.9 no.3
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    • pp.733-741
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    • 2002
  • A class of block designs for general combining ability comparisons within two groups of inbred lines in diallel crosses is given. These block designs are constructed by using balanced block designs obtained by cyclically developing a single initial block. Also, the efficiencies of block designs are tabulated for number of lines 26 or less.

FREQUENCY OF GIEMSA C-BAND CHROMOSOMES IN THREE INBRED LINES OF CHICKENS

  • Yeo, J.S.;Shoffner, R.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.2 no.1
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    • pp.35-38
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    • 1989
  • Giemsa C-banded mitotic chromosome prepatations from White Leghorn, New Hampshire and Rhode Island Red inbred lines were compared for frequency of C-band regions on individual chromosomes. Except for autosomes 3, 6, 8 and 9 and W sex chromosomes, C-banding was extremely variable in other macrochromosomes. No divergence for C-band difference between homologous chromosomes of these lines was detected. Approximately 75% of the mitotic metaphase microchromosomes have recognizable C-band regions with the current technique.

Comparison of Inbred Lines Within Two Groups

  • Park, Kuey-Chung
    • International Journal of Reliability and Applications
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    • v.3 no.3
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    • pp.125-132
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    • 2002
  • Sometimes we have two groups of inbred lines and there are only interest in gca comparisons within two groups of lines of sizes$\P_1$ and $\P_@$, not between two groups. For example, suppose there two Lab, each of the 2 Labs have obtained the best line. For this purpose we now give a method of constructing block designs for diallel cross experiments and we will explain how to calculate efficiency. Then we show the efficiencies in the table.

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Comparative Gene Expression Analysis of Seed Development in Waxy and Dent Corn (Zea mays L.)

  • Sa, Kyu Jin;Choi, Ik-Young;Park, Dae Hyun;Lee, Ju Kyong
    • Plant Breeding and Biotechnology
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    • v.6 no.4
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    • pp.337-353
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    • 2018
  • We used Illumina/HiSeq sequencing for analysis of gene expression profiling among four maize seed types (dent, CM3 and CM6; waxy, CM5 and CM19) at 10 DAP (days after pollination). A total of 88,993,000 (CM3), 103,817,340 (CM6), 103,139,640 (CM5), and 66,978,958 (CM19) sequence reads were generated with read lengths of about 0.9, 1.0, 1.0, and 0.7 billion bp, respectively. We obtained 69.1 (CM3), 71.0 (CM6), 71.2 (CM5), and 71.8% (CM19) high quality reads from the raw data and compared them with reference RNA sequences in a public DB (NCBI). It was revealed that mapped reads were 58%, 63%, 62%, and 62% of the EST reference in CM3, CM6, CM5 and CM19, respectively; and more than 51,000 genes were expressed based on RPKM criteria (over 0.25 value) in each CM3, CM6, CM5, and CM19 inbred line. In differentially expressed gene (DEG) analysis, we found that 3,527 genes were differentially expressed by at least two-fold with 1,709 upregulated in the two waxy inbred lines and 1,818 upregulated in the two dent inbred lines. We also detected genes for the sucrose and starch biosynthesis pathways based on BINs, and different expression patterns between waxy and dent inbred lines were shown for the gene set for starch synthesis, such as sh2, bt2, du1, wx1, and ae1. Although some genes were more expressed in dent lines, most genes for starch synthesis were much expressed in waxy lines. Especially, there was greater expression of the sus2 gene in both waxy lines compared with the dent lines.

The Response of QTL in Generation during Selection (선발과정에서의 세대별 QTL 좌위 고정에 관한 연구)

  • Lee Ji-Woong
    • Journal of Embryo Transfer
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    • v.20 no.3
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    • pp.217-232
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    • 2005
  • The objective of this study was to determine the response of QTL in each generation during selection to develop inbred lines. The simulation program was written in Fortran. Magnitude of QTL effects, base population size, number of QTL assigned to population, and the allelic frequency for the positive allele at each major QTL were highly associated with number of generations to fixation of QTLs during selection. Populations with larger QTL effects and larger base population size had more individuals with fixed QTL. However, a smaller number of QTL assigned to population had a higher fraction of individuals with fixed QTL at each generation compared with more populations with QTL. This simulation study will help to design biological experiments for detection of QTL-marker association using inbred population and to determine optimum number of lines with fixed QTL during inbred line development. To complement this study, additional simulation should be need with abundant replicates, more various population sizes, magnitude of QTL effects, and recombination between markers and QTLs.