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Performance Evaluation of Microorganisms Immobilized Reactive Capping Materials on Elution Blocking of Organic, Nitrogen, and Phosphorus Compounds (미생물이 고정화된 반응성 피복재의 유기물, 질소 및 인 용출 차단성능 평가)

  • Park, Hyungjin;Kim, Young-Kee
    • Journal of Korean Society on Water Environment
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    • v.33 no.4
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    • pp.409-415
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    • 2017
  • This study aims to evaluate the effect of capping materials on blocking pollutant elution from contaminated sediment to water body. Experiments were carried out under conditions in which the elution rate was intensified artificially using compost with high concentration of organic compound and nutrient salts instead of sediments. Activated carbon (AC), modified activated carbon (MAC), P. putida immobilized activated carbon (PBAC) and effective microorganisms immobilized activated carbon (EBAC) were used as capping materials. Zeolite (ZT) and two kinds of commercially available microorganisms immobilized zeolite products (ZC, ZN) were used for comparison experiment. The elution rate of organic compound, nitrogen and phosphorus were compared with that of control experiment. The experiments were conducted for 56 days. Concentrations of chemical oxygen demand, total nitrogen, and total phosphorus were measured to use the comparison of release rate of organic compound, nitrogen and phosphorus. From the experimental results, AC based materials showed better performance to block the elution of organic compound and nitrogen than ZT based materials. Although ZT based materials were more effective than AC and PBAC to block phosphorus, MAC and EBAC showed the best performance of phosphorus elution blocking among the all candidate materials. In conclusion, EBAC is considered as the most effective capping materials, because organic compound, nitrogen and phosphorus will be degraded continuously by EM in the long term.

Effect of Complete Fasting on Energy Metabolites and Serum Parathyroid Hormone and Mineral Excretion in Women (여성에서 절식이 에너지 대사산물 및 혈청 PTH 수준과 무기질 배설에 미치는 영향)

  • 박현서;이은옥
    • Journal of Nutrition and Health
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    • v.34 no.5
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    • pp.547-553
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    • 2001
  • This study was designed to observe the change of body composition and nutrient metabolites and the excretion of minerals during complete fasting for 10 days in thirty women. Average loss of body weight was 7.98kg and body fat was gradually reduced after 9 days fasting, but the loss of lean body mass was reduced after 7 days fasting. Serum glucose level was sustained at constant level, but serum levels of blood urea nitrogen, free acid and $\beta$-hydroxybutyrate were significantly increased during fasting but decreased after re-feeding. Urinary excretions of 3-methylhistidine, total creatinine, and urea were high in the beginning of fasting but gradually decreased. Serum level of parathyroid hormone was significantly reduced by fasting but regained after re-feeding. Serum level of minerals was at the constant level throughout the experimental period. The urinary excretion of minerals(Ca, K, Mg, P) was significantly increased in the early stage of fasting and then reduced from 7 days, but the excretion of Zn was continuously increased until the late stage of fasting. These results showed that amino acid fatty acid released from the breakdown of muscle protein and body fat, respectively, were utilized for energy during fasting. Body weight and BMI were reduced because of the increased muscle protein breakdown and body water excretion during early stage of fasting, but the significant body fat loss was after 9 days fasting. Therefore, it could be suggested to fast for longer than 10 days if the reduction of body fat was planned by fasting, and recommed to exercise and ingest more vitamins and minerals to replace the excreted minerals during fasting. (Korean J Nutrition 34(5) : 547~553, 2001)

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Available Components of Chajogi (Perilla sikokiana B.) at Different Harvest Periods (차조기(紫蘇葉) 채취시기별 유효성분 함량의 변화)

  • Ahn, Hong
    • Food Science and Preservation
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    • v.13 no.6
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    • pp.703-707
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    • 2006
  • The propose of this study was to investigate the changes of available components in Chajogi (Perilla sikokiana B.) by different harvest periods. The Chajogi samples were divided into four groups by harvest period. The four groups were divided into I (harvesting sample at 14, July, 2005), II (harvesting sample at 25, July, 2005), III (harvesting sample at 15, Aug, 2005) and IV sample (harvesting sample at 2, Sept, 2005), and then analysed with regard to the content of proximate compositions, minerals, amino acids, perillaldehyde and anthocyanin. The contents of reducing sugar increased with increasing harvesting periods, while those of crude protein, crude lipid and total amino acids decreased for IV sample as compared to the other samples. The levels of heavy metals in all samples were not detected. The Zn, Fe and Mg contents of IV sample decreased sharply. The perilladehyde contents were 0.36%, 0.42% 0.45%, and 0.35% for I, II, III, and IV products, respectively. III sample as contented to the other samples was shown to have the highest anthocyanine content (1.21% in dry basis). In conclusion, the date of harvesting Chajogi affects the contents of available components.

Dietary Behavior, Health Related Behavior and Nutrient Intake of Female College Students in Chungbuk Area (충북지역 일부 여자대학생들의 건강관련행동과 식행동 및 그에 따른 영양섭취수준 비교)

  • Jung, Eun-Hee
    • The Korean Journal of Community Living Science
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    • v.22 no.4
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    • pp.509-518
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    • 2011
  • This study was intended to investigate dietary behavior, health related behavior and the effects of nutrient intake of female college students. Dietary behavior, health related behavior and the perception of the body conditions were surveyed with anonymous questionnaires, and 24hrs recall method for nutrient intake was obtained from 161 female college students in Chungbuk area. The mean height and weight of the subjects were $161.4{\pm}5.0$cm and $53.8{\pm}7.4 $kg, respectively. The dietary behavior of female college students was generally inadequate. More specifically, irregularity of meals, indifference of dietary balance, and skipping breakfast, showing $4.77{\pm}2.20$for dietary behavior score, $11.76{\pm}3.89$ for DVS and $3.58{\pm}0.82$ for DDS, respectively. It was found that almost 63% of subjects did not anything for health care and the frequency of exercise of subjects was low. The perception of body conditions of the subjects seemed to be generally positive. However, satisfaction levels on body shape was very negative showing that only 12.4% of subjects answered as 'very satisfied' and 'satisfied'. When nutrient intake of the female college students was compared with Dietary Reference Intake for Korean, folate(45%), potassium(55%), vitamin C(59%), Ca(72%), fiber(72%), Fe(79%) and energy(80%) were found to be insufficient. But protein(138%), P(122%) and Na(179%) were found to be oversupplied. The nutrient intake of fiber, plant Ca, vitamin A, ${\beta}$-carotene, vitamin B6, vitamin C, folate and vitamin E were significantly higher, and those of Fe, Zn and protein were a little high in the high score group of dietary behavior. Also Food group intake, DDS and DVS were higher in the high score group of dietary behavior.

Purification and Characterization of a Serine Protease (CPM-2) with Fibrinolytic Activity from the Dung Beetles

  • Ahn, Mi-Young;Hahn, Bum-Soo;Ryu, Kang-Sun;Hwang, Jae-Sam;Kim, Yeong-Shik
    • Archives of Pharmacal Research
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    • v.28 no.7
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    • pp.816-822
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    • 2005
  • Catharsius protease-2 (CPM-2) was isolated from the body of dung beetles, Catharsius molossus, using a three step purification process (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and affinity chromatography on DEAE Affi-Gel blue). The purified CPM-2, having a molecular weight of 24 kDa, was assessed homogeneously by SDS-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of CPM-2 was composed of X Val Gin Asp Phe Val Glu Glu lie Leu. CPM-2 was inactivated by $Cu^{2+}\;and\;Zn^{2+}$ and strongly inhibited by typical serine proteinase inhibitors such as TLCK, soybean trypsin inhibitor, aprotinin, benzamidine, and ${\alpha}_1$-antitrypsin. However, EDTA, EGTA, cysteine, $\beta$-mercaptoethanol, E64, and elastatinal had little effect on enzyme activity. In addition, antiplasmin and antithrombin III were not sensitive to CPM-2. Based on the results of a fibrinolytic activity test, CPM-2 readily cleaved $A{\alpha}-$ and $B{\beta}$-chains of fibrinogen and fibrin, and y-chain of fibrinogen more slowly. The nonspecific action of the enzyme resulted in extensive hydrolysis, releasing a variety of fibrinopeptides of fibrinogen and fibrin. Polyclonal antibodies of CPM-2 were reactive to the native form of antigen. The ELISA was applied to detect quantities, in nanograms, of the antigen in CPM-2 protein.

The Effect of Dehydroepiandrosterone on Isoproterenol-induced Cardiomyopathy in Rats

  • Jeong, Ji-Hoon;Kim, Chan-Woong;Yim, Sung-Hyuk;Shin, Yong-Kyoo;Park, Kyung-Wha;Park, Eon-Sub
    • The Korean Journal of Physiology and Pharmacology
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    • v.10 no.2
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    • pp.79-83
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    • 2006
  • We evaluated therapeutic and preventive properties of dehydroepiandrosterone (DHEA), a weak androgenic steroid, against isoproterenol-induced cardiomyopathy. The cardiomyopathy was induced by daily i.p. administration of isoproterenol to rats for five days. One group of rats were given with daily s.c. for 5 days during isoproterenol and the other group with daily s.c. DHEA for total 10 days, including 5 days before and during isoproterenol. The animals were killed after each treatment, and cardiac muscle failure was evaluated using histopathologic examination and biochemical indices. DHEA was found to reduce the damaged area and inhibit the elevation in the serum levels of glutamic oxaloacetic transaminase (SGOT), lactate dehydrogenase (LDH), skeletal muscle creatine kinase (CK) and heart creatine kinase (CK-MB) induced by isoproterenol. We also assayed widely used oxidative stress parameters, including thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), catalase and glutathion peroxidase (GPx). DHEA decreased the escalated level of TBARS and enhanced the anti oxidant defense reaction with an increase in Mn-SOD and Cu/Zn-SOD. On the other hand, the treatment with DHEA did not affect catalase and GPx activity. The present study indicates that DHEA has a therapeutic and preventive effect against isoproterenol-induced cardiomyopathy and its effects may depend largely on the increase in SOD activity.

Role of modifiers on the structural, mechanical, optical and radiation protection attributes of Eu3+ incorporated multi constituent glasses

  • Poojha, M.K. Komal;Marimuthu, K.;Teresa, P. Evangelin;Almousa, Nouf;Sayyed, M.I.
    • Nuclear Engineering and Technology
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    • v.54 no.10
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    • pp.3841-3848
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    • 2022
  • The effect of modifiers on the optical features and radiation defying ability of the Eu3+ ions doped multi constituent glasses was examined. XRD has established the amorphous nature of the specimen. The presence of various functional/fundamental groups in the present glasses was analyzed through FTIR spectra. The physical, structural and elastic traits of the glasses were explored. The variation in the structural compactness of the glass structure according to the incorporated modifier was enlightened to describe their suitability for a better shielding media. For the examined glasses, the metallization criterion value varied in the range 0.613-0.692, indicating the non-metallic character of the glasses with possible nonlinear optical applications. The computed elastic moduli expose the Li-containing glass (BTLi:Eu) to be tightly packed and rigid, which is a requirement for a better shielding channel. Furthermore, the optical bandgap and the Urbach energy values are calculated based on the optical absorption spectra. The evaluated bonding parameters revealed the nature of the fabricated glasses covalent. In addition, we investigated the radiation attenuation attributes of the prepared Eu3+ ions doped multi constituent glasses using Phy-X software. We determined the linear attenuation coefficient (LAC) and reported the influence of the five oxides Li2O3, CaO, BaO, SrO, and ZnO on the LAC values. The LAC varied between 0.433 and 0.549 cm-1 at 0.284 MeV. The 39B2O3-25TeO2-15Li2O3-10Na2O-10K2O-1Eu2O3 glass has a much smaller LAC than the other glasses.

Elemental characteristics of sialoliths extracted from a patient with recurrent sialolithiasis

  • Buyanbileg Sodnom-Ish;Mi Young Eo;Kezia Rachellea Mustakim;Yun Ju Cho;Soung Min Kim
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.50 no.2
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    • pp.94-102
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    • 2024
  • The exact mechanism of sialolith formation has yet to be determined. Recurrence of sialolithiasis is rare, affecting only 1%-10% of patients. The current study presents a case of recurrent stones that occurred twice on the right submandibular gland 6 months postoperative and 7 months after reoperation in a 48-year-old female patient. The stones were analyzed using histology, scanning electron microscopy, energy dispersive spectroscopy, and transmission electron microscopy (TEM). The first stone showed a three-layered structure with a poorly mineralized peripheral multilayered zone, highly mineralized middle layer, and the central nidus. The stones were composed of Ca, C, O, Cu, F, N, P, Si, Zn, and Zr. In TEM, compact bi-layered bacterial cell membrane was found on the peripheral layer and the central nidus of the stone as well as exosomes in the central nidus. The results demonstrated the essential components of sialolith formation, including bacteria, inflammatory exosomes, and exfoliated salivary epithelial cells that cooperatively underwent the pathogenetic progresses of central nidus formation, induction of compact zone calcification of the middle layer, and repeated subsequent deposition in the peripheral multilayer zone. The rapid recurrence could have resulted from residual pieces of a sialolith acting as the nidus of bacterial infection.

Study on the Change of Nutrients Contents according to the Use of various Multi-Functional Grinder (믹서의 종류에 따른 영양소 함량 변화에 대한 연구)

  • Choi, Byung Bum;Kim, Chun Huem;Kim, Young Soon
    • Culinary science and hospitality research
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    • v.20 no.6
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    • pp.262-274
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    • 2014
  • In this study, changes of nutrients such as vitamins, minerals, and antioxidants were determined by using a multi-functional grinder. Contents of vitamin C and vitamin $B_1$ were measured by HPLC, and antioxidant activity was estimated toward free radical scavenging effect in using DPPH. Contents of minerals(Zn, Mn, Fe, Mg, K, and Ca) were computed by ICP-Mass. Results identified that the contents of vitamin C was $44.06{\pm}10.86mg/kg$ in mandarine, $132.1{\pm}22.80mg/kg$ in orange, and $12.79{\pm}2.01mg/kg$ in pineapple by using the hand blender for 3 minutes, and the loss rate of vitamin C contents were 12%, 7%, and 12% in comparison with the control group. In addition, the contents of vitamin C were $41.89{\pm}5.55mg/kg$ in mandarine, $131.51{\pm}12.67mg/kg$ in orange and $16.76{\pm}1.47mg/kg$ in pineapple when using of the grinder for 3 minutes, and the loss rate of vitamin C contents were 16%, 8%, and 17%. The results of vitamin $B_1$ showed a tendency to decrease in the same manner as the content of vitamin C even if there was no significant difference. Furthermore, the result of antioxidant activity revealed that free radical scavenging effect of sample was 60% decreased when using a hand blender and 10% decreased when using a grinder. Thus decrease rate of antioxidant activity when using the hand blender was higher than grinder. Lastly, current study could find any significant differences among the 16 food samples for cooking when employing the multi-functional grinder(p<0.05).

Comparison of Nutritional Composition in Korean Rices (쌀 품종별 백미와 현미의 영양성분 조성 비교)

  • 최정숙;안훈희;남희정
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.5
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    • pp.885-892
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    • 2002
  • The purpose of this research was to prove the excellency of unpolished (brown) rice with respect to nutritional composition and to compare chemical composition among six varieties of rice. Some nutritional composition in the Polished (well -milled) rice and unpolished (brown) rice of the six typical Korean varieties (Chucheong, Hwasung, Odae, Ilmi, Dongjin, Ilpum) were determined. Most of nutrients in the brown rice were much greater than those in the milled rice and there was significant difference among rice varieties in some chemical compositions. The major minerals of milled rices were Ca, 6~15 mg%;P, 91~125mg%;Fe, 0.3~1.2mg%; Na, 10~14mg%;K, 106~205 mg%; Zn, 1.0~1.8mg%; and Mg, 32~58 mg%. The mineral contents of brown rices wre Ca, 4~11mg%;P, 92~286mg%;Fe, 1.3~1.9mg%;Na, 12~15mg%;K, 243~320mg%;Zn, 1.5~2.3mg%;Mg, 112~140mg%. Major fatty acids in six rice varieties were linoleic acid, oleic acid and palmitic acid, which comprised of about 93% of total fatty acid. Amino acid analysis showed that aspartic acid, glutamic acid, arginine and leucine were major amino acids, whereas histidine, threonine and tryptophan were minor ones of rice. Most ammo acid contents was higher in Ilmi than the other varieties. The contents of vitamin in brown rices were considerably higher than those in well-milled rice. There was not significant difference in total dietary fiber among rice varieties : 1.1~1.2% for milled rice,3.2~3.5% for brown rice.