• Asian-Australasian Journal of Animal Sciences
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• v.15 no.12
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• pp.1695-1701
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• 2002

The purposes of this study were to optimize the in vitro maturation (IVM) and culture (IVC) systems of rabbit oocytes. Cytoskeletal structures in the germinal vesicle stage (GV) and during IVM are also investigated. Ovaries were transported from local slaughterhouses and the cumulus-oocyte complexes (COCs) were collected from ovarian follicles (${\geq}1mm$). COCs were randomly allocated to TCM199-based medium ($T_1$, TCM-199) supplemented with $NaHCO_3$, glucose, sodium pyruvate and FSH ($T_2$), $T_2+E_2+LH$ ($T_3$), $T_3+FBS$ ($T_4$), or $T_1+E_2+LH+FSH+FBS$ ($T_5$), for IVM. In Experiment 1, COCs were retrieved from the follicles and 51 GV oocytes were fixed in the fixative (MTSB-XF) for nuclear and cytoplasmic examinations. In Experiment 2, progressive changes of both the nucleus and the cytoskeleton were examined at 0, 6, 16, and 20 h after IVM. Maturation (MR) and developmental rates were assessed in Experiment 3. Cytoplasmic microtubules (MT) were clearly observed in rabbit GV oocytes. To our knowledge, this is the first report that describes the appearance of MT structures in the GV stage ooplasm. Tremendous variations in cytoskeletal alterations were observed among treatments with the exception of the vitelline ring (VR), which is constantly visible and unchanged during maturation. Germinal vesicle breakdown (GVBD) does not occur at 6 h after onset of maturation culture. When the oocytes for IVM were collected within 2 h, results from Experiment 3 showed that rates of nuclear maturation were 42, 8, 42, 37 and 65% at 16 h of IVM for $T_1$ through $T_5$, respectively, in which $T_1$, $T_4$ and $T_5$ had significantly greater MR than those in other groups (p<0.05). Morula/blastocyst development after parthenogenetic activation ranged from 20 to 63% with significantly greater rates in $T_3$, $T_4$ and $T_5$ (p<0.05). These results suggested that oocytes recovered from slaughterhouse ovaries can be matured and parthenogenetically activated in vitro, but the MR remained low in this study. Addition of $E_2$ and LH in the medium may be beneficial for cytoplasmic maturation, but FBS exerts a nega- tive role in the subsequent development of parthenogenetic embryos when energy substrates are provided in the IVC media. More studies are required for improving the MR and further development of the GV stage rabbit oocytes.

• Journal of Korean Academy of Nursing
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• v.28 no.1
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• pp.132-142
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• 1998

This study was conducted to investigate whether exercise therapy applied in an efficacy expectation promoting program based on the self-efficacy theory of Bandura(1977) would increase self-efficacy and metabolism in NIDDM patients. The study design was a nonequivalent control group pre-test post-test quasi-experimental desist The exercise therapy applied in the efficacy expectation promoting program was composed of a staged exercise program, a small booklet relating personal experience with diabetes mellitus and a telephone coaching program on performance accomplishment. vicarious experience and verbal persuasion, which are all induction modes of efficacy expectation. The subjects of the study were twenty eight NIDDM patients who received follow-up care regularly through the out-patient department of endocrine medicine in one general hospital which had a diabetic clinic. Fourteen were assigned to the experimental group and fourteen to the control group. The experimental group participated in the exercise therapy applied in the efficacy expectation promoting program from three to five times per week for four weeks and the control group did not have the program. The collected data were analyzed using the X²-test, t-test, paired t-test, and Cronbach's Alpha using SPSS /PC/sup +/. The results are summarized as follows 1. Experimental group had higher efficacy score than control group(t=5.98, p=.00). And. There was a significant different in the efficacy score before exercise therapy applied in the efficacy expectation promoting program and after in experimental group(t=-6.42, p=.00). 2. Experimental group did not have lower level of glucose metabolism than control group(FBS : t=.32, p=.75, HbAlC : t=.60, p=.55, pc 2hrs. glucose : t=-.29, p=.78). But, There was a significant different in the aunt of glucose metabolism before exercise therapy applied in the efficacy expectation promoting program and after in experimental group(FBS : t=3.63, p=.003, HbAlC t=4.20, p=.00, pc 2hrs. glucose : t=1.93, p=.001). 3. Levels of lipid metabolism were partly a significant different between Experimental group and control group(triglyceride t=-1.87, p=.07, HDL cholesterol : t=-.29, p=.77. body weight : t=1.78, p=.09, Total cholesterol : t=-2.17, p=.04). And, There was partly a significant different in the amount of lipid metabolism before exercise therapy applied in the efficacy expectation promoting program and after in experimental group(triglyceride : t=2.50, p=.03, HDL cholesterol : t=-.43, p=.67, body weight : t=5.34. p=.00, Total cholesterol : t=2.26, p=.04). In conclusion, it was found that exercise therapy applied in an efficacy expectation promoting program was an effective nursing intervention for increasing self-efficacy and metabolism.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.4
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• pp.23-29
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• 2017

This experiment was carried out to compare the productivity and feed value between standard cultivation and organic mixed expeller cake fertilizer cultivation to develop organic cultivation technology except from standard cultivation using chemical fertilizers in the field of forage production. This study was conducted in the rice field of Livestock Research Institute Jacheon-myeon, Gangin-gun Jellanam-do. Organic mixed expeller cake fertilizer consists of 4.0% nitrogen, 65% castor, 30% seeds and 5% fish meal. Sorghum-Sudangrass Hybrids(SX17) was sowed 40kg per hectare on May 25. Test group formation was consisted of a standard group and test treatment. The standard group(Con.) was fertilized chemical fertilizer according to the N-P-K kg/ha, 200-150-150 (basal 100kg, uptake 100kg). Test treatment was carried out organic fertilizer 100%(T1), organic fertilizer 150%(T2) and organic fertilizer 200%(T3) according to the nitrogen content of standard group(Con.). Plant height of harvest time depending on the treatment was that Con was 317cm, T1 was 252cm, T2 was 269cm and T3 was 293cm. Dry matter yield was that Con was 11,386kg/ha, T1 was 6,015kg/ha, T2 was 6,799kg/ha, T3 was 7,627kg/ha. In the test treatment, plant height and dry matter yield increased with increasing fertilizer. Crude protein was the height in Con(7.0%) and Crude protein of test treatment was T1(5.7%), T2(6.3%), and T3(5.1%). As a result of this study, T2 was recommended.

• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.624-630
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• 2008

Terminal restriction fragment length polymorphism (T-RFLP) is a method that has been frequently used to survey the microbial diversity of environmental samples and to monitor changes in microbial communities. T-RFLP is a highly sensitive and reproducible procedure that combines a PCR with a labeled primer, restriction digestion of the amplified DNA, and separation of the terminal restriction fragment (T-RF). The reliable identification of T-RF requires the information of nucleotide sequences as well as the size of T-RF. However, it is difficult to obtain the information of nucleotide sequences because the T-RFs are fragmented and lack a priming site of 3'-end for efficient cloning and sequence analysis. Here, we improved on the T-RFLP method in order to analyze the nucleotide sequences of the distinct T-RFs. The first method is to selectively amplify the portion of T-RF ligated with specific oligonucleotide adapters. In the second method, the termini of T-RFs were tailed with deoxynucleotides using terminal deoxynucleotidyl transferase (TdT) and amplified by a second round of PCR. The major T-RFs generated from reference strains and from T-RFLP profiles of activated sludge samples were efficiently isolated and identified by using two modified T-RFLP methods. These methods are less time consuming and labor-intensive when compared with other methods. The T-RFLP method using TdT has the advantages of being a simple process and having no limit of restriction enzymes. Our results suggest that these methods could be useful tools for the taxonomic interpretation of T-RFs.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.5
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• pp.664-671
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• 2020

A Smart Building, one that uses automated processes to control its operations, refers in this study to one that uses both Internet of Things (IoT) devices and cloud services software. Cloud service providers (e.g. Amazon, Google, and Microsoft) have recently providedIoT cloud platform application services on IoT devices. According to Postscapes, there are now 152 IoT cloud platforms. Choosing one for a smart building is challenging. We selected Microsoft Azure IoT Hub and Amazon's AWS (Amazon Web Services) IoT. The two platforms were evaluated and selected from a smart building perspective. Each prototype was evaluated on two different IoTplatforms, assuming a typical smart building scenario. The selection was based on information and experience gained from developing the prototype system using the IoT cloud platform. The assessment made in this evaluation may be used to select an IoTcloud platform for smart buildings in the future.

• Journal of Korea Foundry Society
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• v.1 no.2
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• pp.10-18
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• 1981

In this paper tensile strength and fatigue propagation rate were investigated by aging treatment $(T_6)$ and thermomechanical treatment (TMT) of 7075-Al Alloy specimen. The results of teat showed that TMT improved tensile strength and fatigue crack propagation due to bomogenization of microstructure. In TMT, the results of comparison between T-H' AHA and T-AHA and $T-AH{\cdot}{\cdot}{\cdot}$and $T-HA{\cdot}{\cdot}{\cdot}$were showed that T-AHA and $T-AH{\cdot}{\cdot}{\cdot}$treatments, after solution treatment, which are aging treated before rolling have higher tensile strength. Our investigation on high temperature stability at the Specimen for $T_6$, T-AHA, $T-AH{\cdot}{\cdot}{\cdot}$treatments resulted in rapid reduction of tensile strength over $150^{\circ}C$, but the reduction of tensile strength for specimen of TMT was smoothed than $T_6$.

• East Asian mathematical journal
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• v.24 no.1
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• pp.81-95
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• 2008

Let E be a uniformly convex Banach space with a uniformly $G{\hat{a}}teaux$ differentiable norm, C a nonempty closed convex subset of E, and $T\;:\;C\;{\rightarrow}\;E$ a nonexpansive mapping satisfying the weak inwardness condition. Assume that every weakly compact convex subset of E has the fixed point property. For $f\;:\;C\;{\rightarrow}\;C$ a contraction and $t\;{\in}\;(0,\;1)$, let $x_t$ be a unique fixed point of a contraction $T_t\;:\;C\;{\rightarrow}\;E$, defined by $T_tx\;=\;tf(x)\;+\;(1\;-\;t)Tx$, $x\;{\in}\;C$. It is proved that if {$x_t$} is bounded, then $x_t$ converges to a fixed point of T, which is the unique solution of certain variational inequality. Moreover, the strong convergence of other implicit and explicit iterative schemes involving the sunny nonexpansive retraction is also given in a reflexive and strictly convex Banach space with a uniformly $G{\hat{a}}teaux$ differentiable norm.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2014.10a
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• pp.358-360
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• 2014

For the several decades, various nanomaterials are broadly used in industry and research. With the growth of nanotechnology, the study of nanotoxicity is being accelerated. Particularly, mercury ion is widely used in real life. Because the mercury is representative high toxic material, it is highly recommended to detect the mercury ion. In previous reported work, thymine-thymine mismatches (T-T) capture mercury ion and create very stable base pair ($T-Hg^{2+}-T$). Here, we performed the high sensitive sensing method for direct label free detection of mercury ions and DNA binding using Kelvin Probe Force Microscope (KPFM). In this method, 30 base pairs of thymine (T-30) is used for mercury specific DNA binding ($T-Hg^{2+}-T$). KPFM is able to detect the mercury ion because there is difference between bare T-30 DNA and mercury mediated DNA ($T-Hg^{2+}-T$).

• Journal of the Korean Society for Heat Treatment
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• v.27 no.6
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• pp.288-294
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• 2014

The study on the migration of carbon in T23/T91 dissimilar metal welds for the boiler was conducted at $600^{\circ}C$ with an increase of aging time from 1 to 100 hrs. Following results were obtained. The heavily etched dark band tended to increase from a molten boundary owing to an increase of aging time, which leads to form hard (T91) and soft (T23) zones with different hardness. It was found that carbon was migrated from T23 area to T91 area due to different carbon activities. In addition, soft and hard zones were formed in regional area mainly resulted from carbide dissolutions and precipitates.

• Journal of Radiation Industry
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• v.10 no.4
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• pp.227-230
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• 2016

DWI of biological effects are independent of magnetic field strength in various regions. High field strength, however, does affect the signal to noise ratio (SNR) and artifacts of diffusion weighted imaging (DWI) images, which ultimately will influence the quantitative of diffusion imaging. In this study, the effects of field strength on DWI are reviewed. The effects of the diseases also are discussed. Comparing DWI in cerebellum, WM, GM, Hyperacute region measurements both as a function of field strength (1.5T and 3.0T). Overall, the SNR of the DWI roughly doubled going from 1.5 T to 3.0 T. In summary, DWI studies at 3.0 T is provided significantly improved DWI measurements relative to studies at 1.5T.