• The Journal of the Korean bone and joint tumor society
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• v.3 no.1
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• pp.9-17
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• 1997

Resistance to combination chemotherapy remains challenge in the treatment of osteosarcoma. One of the mechanisms of multiple drug resistance is an increased expression of the multidrug resistance gene(mdr1). Expression of the P-glycoprotein(mdr-1 gene product) was studied immunohistochemically and the mdr-1 gene by in situ hybridization in 33 osteosarcomas relating to various prognostic factors. Thirty cases out of 33 osteosarcomas(90.9%) showed positive cytoplasmic reactions with P-glycoprotein and nineteen instances(57.6%) were strong positive(2+). The older(>20 years) and female patients revealed more intense immunohistochemical reactions rather than those of the younger and male patients. Osteoblastic and chondroblastic osteosarcomas revealed more strong immunohistochemical reactions compared to fibroblastic types. There were no significant staining differences between the type of bony involvement, Broder's grade and the presence of necrosis. On follow-up, the mean survival rate was decreased in the strong positive group, however, this was not statistically significant. In situ hybridization for mdr-1 gene revealed positive signals in 22 cases out of 29 osteosarcomas(75.9%). Chemotherapy was done in 15 cases out of 28 patients(53.6%). The results of immunohistochemistry and in situ hybridization were not correlated with the protocols for chemotherapy. However, this result should be confirmed by a larger scale study about mdr1 mRNA expression.

• The Korean Journal of Zoology
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• v.37 no.3
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• pp.304-310
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• 1994

Using in situ hybridization, we have mapped the anatomical localization of perikarya containing myNA that codes for sonadotropin releasing hormone (GnRH) in the brains of female frogs, R. dybowskii. DNA olisomers, with sequences complementary to the GnRH portion of pro-GnRH myNA sequence, were synthesized and hybridized to paraformaldehvde-fixed, sagittal sections of the whole brain stem. The distribution of the GnRH mRNA containing cell bodies was similar to that described for GnRH peptide by immunohistochemistrv. That is, cells containing GnRH mRNA were observed in the medial septal area, anterior preoptic area, ventromedial hvpothalamus and infundibular regions. However, another cell groups which contains GnRH mRNAs were also detected by in situ hybridization in the bed nucleus of hippocampal commissure, preoptic area, nucleus infundibularis dorsalis, mesencephalic nuclei and intermediolateral cell column of spinal cord areas. These results demonstrate the feasibility of using in situ hybridization as a strategy to study the distribution of GnRH neurons and the detection of GnRH gene expression in the vertebrates.

• Journal of Genetic Medicine
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• v.2 no.2
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• pp.79-81
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• 1998

Genetic amniocenteses were performed in a series of 127 patients as a routine study. Samples from the patients were cultured by in situ method, flask method or both according to the state of amniotic fluid. The overall success rate of culture was 97.6% and no culture failure was observed in the flask method. It took 5 days first of all and 8.15 days average from set-up to harvest and there were 7.2 colonies per dish in in situ method. Therefore, it is suggested that in situ method which decreased the mean culture days and made clonal analyses possible, is a clinically available and even more reliable method in parallel with flask method in prenatal diagnosis.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.03a
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• pp.65-84
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• 2000

Europe and US which have more restrictive regulations than Korea about the noise and vibration during construction are using Auger-cast Pile to reduce the problem relating with noise and vibration. However Auger-cast Pile has problems like difficult quality control and low bearing capacity. In Europe, Displacement in-situ concrete Pile has been used to sove that problems since 1990s, and Korea has performed the test construction in 1997 and it has been used as the real structural foundation since 1998. Test and real construction results verified that the allowable capacity of the pile(diameter = 410mm) is between 70 and 100ton. Though De Beer & Van Imps design method utilizing CPT result is used to calculate the bearing capacity of the Displacement in-situ Pile, Korea is dependant upon the SPT as the sounding test, so design method utilizing SPT result is necessary to promote the application of the pile. To find out reasonable design method using SPT result, rearing capacity of the pile constructed in sand and clay in Korea was calculated using Meyerhof, SPT-CPT translation method, Nordlund, Douglas and DM-7 method, and the calculation results were compared to the load test result. Analysis result shows that SPT-CPT translation method is more reliable than others and economical design can be possible because it considers efficiently the friction capacity of Displacement in-situ Pile.

• Atmosphere
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• v.14 no.2
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• pp.7-10
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• 2004

A short review on TOTO (TOtable Tornado Observatory), one of the earliest in-situ observing systems for tornado, is provided. TOTO was outfitted with sensors for measuring wind, pressure and humidity, and storm researchers, in mid-1980's, tried to put it inside tornadoes for detailed studies on tornado, but failed. However, the accumulated knowledge and experience with TOTO lead to a successful field program in mid-1990's. A story about DOROTHY, a parody of TO TO in the movie "Twister!", is also provided.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1994.11a
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• pp.54-58
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• 1994

H$_2$ bake and GeH$_4$ clean are used as a in-situ pre-clean method in low temperature Si based epitaxial growth technology using rapid thermal processing chemical vapor deposition (RTPCVD). In this paper, the H$_2$ bake and GeH$_4$ clean processes are optimized for low surface defect density using Taguchi method. In H$_2$ bake process, the epitaxial growth temperature affects dominantly on the surface defect density, and the next affecting factors are H$_2$ bake temperature and rinse time in de-ionised water. In GeH$_4$ clean process, GeH$_4$ clean temperature affects most strongly on the surface defect density, and the minor factor is GeH$_4$flow rate. The optimum process conditions predicted fly Taguchi method agree well with tile experimental data in both in-situ clean processes.

• Nuclear Engineering and Technology
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• v.51 no.3
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• pp.867-876
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• 2019

Second harmonic generation using nonlinear ultrasonic waves have been shown to be an early indicator of possible fatigue damage in nuclear power plant components. This technique relies on measuring amplitudes, making it highly susceptible to variations in transducer coupling and instrumentation. This paper proposes an experimental procedure for in-situ surface wave nonlinear ultrasound measurements on specimen with permanently mounted transducers under high cycle fatigue loading without interrupting the experiment. It allows continuous monitoring and minimizes variation due to transducer coupling. Moreover, relations describing the effects of the measurement system nonlinearity including the effects of the material transfer function on the measured nonlinearity parameter are derived. An in-situ high cycle fatigue test was conducted using two 304 stainless steel specimens with two different excitation frequencies. A comprehensive analysis of the nonlinear sources, which result in variations in the measured nonlinearity parameters, was performed and the effects of the system nonlinearities are explained and identified. In both specimens, monotonic trend was observed in nonlinear parameter when the value of fundamental amplitude was not changing.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.4
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• pp.697-703
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• 2006

In this research, model tests for in-situ Top-Base Foundation are carried out in other to investigate the load delivering mechanism and the incremental effect of bearing capacity. According to the result of model tests, the load-settlement curves of both in-situ Top-Base(In-situ TBF) and Precast Top-Base Foundation(PC-TBF) showed similar results in term of the ground movement and effect of bearing capacity. Also, the range of vertical stresses delivered into ground was decreased with Top-Base method regarding other types foundations.

• Journal of Ocean Engineering and Technology
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• v.24 no.1
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• pp.99-105
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• 2010

The concentration and size analysis of in-situ suspended particulate material were measured using an optical instrument, LISST-100, in the bottom layer at the three inlets of the Seomjin River Estuary, mouth of Gwangyang bay, and Gwangyang bay-side of the Namhae Bridge. In the Seomjin river estuary and mouth of Gwangyang bay-side of the Namhae Bridge, the in-situ mean grain size of the suspended material changed from a uni-modal distribution with a dominant peak at a coarse fraction to a bi-modal distribution with a secondary peak at the finer particles. Seomjin River. The interactions between suspended particulate concentration and beam attenuation coefficient of suspended particulate matters depended on the supply of finer and coarser particles in the mouth of Gwangyang Bay and Seomjin River. So, interactions reflected difference of the concentration, mean size and sorting of suspended particulate matters. The difference of interaction showed dynamic behaviors to the resuspension and deflocculation processes increased river discharge. This showed that it may be possible to use the interactions between the suspended particle concentration and beam attenuation coefficient to monitor the temporal and spatial distributions of in-situ particles.

• Analytical Science and Technology
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• v.8 no.4
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• pp.723-730
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• 1995

Cyclic voltammetry and in situ STM were employed to examine the interfacial structures of a Pt(111) electrode in 0.1 mM KCN (pH9.5) and 0.1 mM KSCN (pH7) solutions. In situ STM atomic resolution revealed well ordered (2${\surd}$3${\times}$2${\surd}$3)$R30^{\circ}$-6CN and ($2{\times}2$)-2SCN structures within the double layer charging region. Six CN adsorbates formed a hollow hexagon, which embraced a coadsorbed $K^+$ cation. In contrast, the coadsorbed $K^+$ cations on the SCN covered Pt(111) were poorly ordered, despite adsorbed SCN formed a long range ordered ($2{\times}2$)-2SCN adlattice. In situ STM revealed the pronounced influence of potential in controlling the structures of compact layers at the proximity of a Pt electrode. Cathodic polarization facilitated the replacement of the coadsorbed cations by protons.