• Title/Summary/Keyword: In-Situ experiment

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Limiting Nutrients for Phytoplankton Growth in the Seomjin River Estuary as Determined by Algal Bioassay Experiment (생물검정실험에 의한 섬진강 하구역 식물플랑크톤 성장의 제한영양염 평가)

  • Kwon Kee Young;Kim Chang Hoon;Kang Chang Keun;Moon Chang Ho;Park Mi Ok;Yang Sung Ryull
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.5
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    • pp.455-461
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    • 2002
  • limiting nutrients for phytoplankton were determined by dissored inorganic nitrogen/phosphorous (DIN/DIP) in situ and algal bioassay experiment in the Seomjin River estuary during a study period from March 1999 to October 2001. DIN/DIP ranged from 14.7 to 681.1 during the study period. DIN/DIP was over 16 at the upper and middle estuarine region where salinity was lower than 25 psu and chlorophyll a concentration was high, probably indicating P-limitation in this region while the ratio was less than 16 at the high saline (> 25 psu) region, reflecting the supply of DIP from Gwangyang Bay and thereby indicating N-limitation at the lower estuarine region. These results suggested that the spatial distribution of DIN/DIP in the study region was controlled by the high supply of phosphate from Gwangyang Bay, the low input from Seomjin River and the active uptake by phytoplankton within the estuarine system. The bioassay experiments using Skeletonema costatum, Thalaasiosira rotula and in situ phytoplankton assemblage displayed relatively higher growth of phytoplankton in the P-added culture media, indicating P-limitation. This result was well consistent with the spatial distribution of inorganic nutrients. S. costatum showed a rapid adaptation to the low salinity compared to other phytoplankton species. This phenomenon seemed to account for the strong (> $90\%$ in total cell number) S. costatum bloom in autumn in this estuary. Moreover, although phytoplankton growth rate was higher in the P-added culture media at the end of culture experiment of in situ phytoplankton, the fast growth in the trace metal-added media at the beginning of the experiment suggested a possibility of limitation by other micro-nutrients such as trace metal and vitamin etc.

Microscopic damping mechanism of micro-porous metal films

  • Du, Guangyu;Tan, Zhen;Li, Zhuolong;Liu, Kun;Lin, Zeng;Ba, Yaoshuai;Ba, Dechun
    • Current Applied Physics
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    • v.18 no.11
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    • pp.1388-1392
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    • 2018
  • Metal thin films are used widely to solve the vibration problem. However, damping mechanism is still not clear, which limits the further improvement of the damping properties for film and the development of multi-functional damping coating. In this paper, Damping microscopic mechanism of porous metal films was investigated at both macroscopically and microscopically mixed levels. Molecular dynamics simulation method was used to model and simulate the loading-unloading numerical experiment on the micro-pore and vacancy model to get the stress-strain curve and the microstructure diagram of different defects. And damping factor was calculated by the stress-strain curve. The results show that dislocations and new vacancies appear in the micro-pores when metal film is stretched. The energetic consumption from the motion of dislocation is the main reason for the damping properties of materials. Micro-mechanism of damping properties is discussed with the results of in-situ experiment.

Enhanced In-situ Mobilization and Biodegradation of Phenanthrens from Soil by a Solvent/Surfactant System

  • Kim, Eun-Ki;Ahn, Ik-Sung;L.W.Lion;M.L.Shuler
    • Journal of Microbiology and Biotechnology
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    • v.11 no.4
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    • pp.716-719
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    • 2001
  • The mobilization and biodegradation of phenanthrene in soil was enhanced by using paraffin oil, which was stabilized by the addition of a surfactant (Brji 30). The ratio of paraffin oil/Brij 30 was determined by measuring the change in the critical micelle concentration. When only surfactant was used, the stabilized paraffin oil emulsion could dissolve more phenanthrene in the water phase. Column experiment showed increased phenanthrene mobilization from the contaminated soil. The phenanthrene mobilized in the paraffine oil/Brij 30 emulsion was biodegraded faster than that in water phase or surfactant solution. This result indicates that a paraffin oil/surfactant system can be effectively used for the removal of PAH from contaminated soil.

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Combined Effect of Fluorouracil with Metoclopramide or Propantheline Bromide in Rats and Rabbits (Fluorouracil과 Metoclopramide 및 Propantheline.Bromide와의 병용시(倂用時) 항암효과(抗癌効果)에 관한 연구(硏究))

  • Choi, Jun-Shik;Lee, Jin-Hwan;Baik, Chai-Sun
    • Journal of Pharmaceutical Investigation
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    • v.14 no.1
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    • pp.11-18
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    • 1984
  • This paper was attempted to study on the effect of metoclopramide or propantheline on the absorption rate and bioavailability and pharmacokinetic parameter of fluorouracil in rats and rabbits. The result are as follows; Metoclopramide increased the absorption rate of fluorouracil but propantheline decreased in situ experiment with rat small intestine. Metoclopramide increased the blood level and relative bioavailability and absorption rate constant of fluorouracil in rabbits. Prolonged the peak blood level (tmax) and decreased the absorption rate constant (Ka) but did not affect the blood level and relative bioavailability of fluorouracil in rabbits. As a matter of fact, it is considerd that the coadministration of metoclopramide or propantheline is more desirable than the single administration of fluorouracil for available dosage regimen.

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OVERVIEW OF KOMPSAT APPLICATION PRODUCT VALIDATION SITE AND THE RELATED ACTIVITIES

  • Lee, Kwang-Jae;Youn, Bo-Yeol;Kim, Duk-Jin;Kim, Youn-Soo
    • Proceedings of the KSRS Conference
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    • 2007.10a
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    • pp.122-125
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    • 2007
  • In recent years, there has been an increasing demand for improved accuracy and reliability of Earth Observation Satellite (EOS) data. Most of the data users in the field of remote sensing require understanding of product accuracy and uncertainty. Especially, EOS application products should be validated for practical application in the field. In order to evaluate the availability and applicability of application products, it will be necessary to establish a systematic validation system including techniques, equipments, ground truth data, etc. The Product Validation Site (PVS) for generation and validation of KOMPSAT application products was designed and established with various in-situ equipment and dataset. This paper presents the status of PVS and summarizes some results from experiment studies at PVS.

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Electron Holography of Advanced Nanomaterials

  • Shindo, D.;Park, H.S.;Kim, J.J.;Oikawa, T.;Tomita, T.
    • Applied Microscopy
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    • v.36 no.spc1
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    • pp.63-69
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    • 2006
  • By utilizing a field emission gun and a biprism installed on a transmission electron microscope (TEM), electron holography is extensively carried out to visualize the electric and magnetic fields of nanomaterials. In the electric field analysis, the distribution of electric potential in a sharp tip made of W coated with $ZrO_2$ is visualized by applying the voltage to the tip. Denser contour lines due to the electric potential are observed with an increase in the bias voltage. In the magnetic field analysis by producing the strong magnetic field with a sharp magnetic needle made of a permanent magnet, the in situ experiment is carried out to investigate the magnetization of hard magnetic materials. The results of these experiments clearly demonstrate that electron holography is a promising advanced transmission electron microscopy technique to characterize the electric and magnetic properties of nanomaterials.

In Situ Localization of Rice dwarf phytoreovirus P12 Protein in Infected Rice Plant (벼오갈병 바이러스 P12 단백질의 벼 감염세포 내 소재양식)

  • Lee Bong-Choon;Hong Yeon-Kyu;Hong Sung-Jun;Park Sung-Tae
    • Research in Plant Disease
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    • v.12 no.1
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    • pp.25-27
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    • 2006
  • Rice dwarf phytoreovirus (RDV), a member of the family Reoviridae has a genome composed of 12 segmented dsRNAs designated as 51 to 512 with an increasing order of mobility in polyacrylamide gel electrophoresis (PAGE). RDV encode 12 structural and non-structural proteins, $P1{\sim}P12$ which are encoded by the $S1{\sim}S12$ segments of the dsRNA genome, respectively. In this experiment, we confirmed in situ localization of RDV particles and P12 in cytoplasm of infected rice plant. We observed specific reaction of the gold particles using virus particle and P12 protein specific antiserum with protein A-gold immunolabelling in electron microscope. It was observed that gold particles specifically react to virus particles in cytoplasm in case using the antiserum for virus particles. In the case of antiserum for P12 protein, gold particles sporadically existing on cytoplasm without existing in organelle of cytoplasm specifically. As this result, RDV P12 protein encoded by S12 located in cytoplasm.

Pharmaceutical Study on Pranoprofen Argininate and Pranoprofen Lysinate (Pranoprofen Argininate 및 Pranoprofen Lysinate의 약제학적 연구)

  • Jee, Ung-Kil
    • Journal of Pharmaceutical Investigation
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    • v.17 no.4
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    • pp.197-204
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    • 1987
  • Novel pranoprofen algininate and lysinate salts were manufactured and their salt formation was confirmed by melting point, infrared spectroscopy, nuclear magnetic resornance spectroscopy, differential scanning calorimetry and powder X-ray diffractometry. The physical properties of pranoprofen lysinate and argininate salts were compared with those of pranoprofen through in vitro and in vivo tests. Solubility, $pK_a$ and lipid-water partition coefficient were measured through in vitro experiments, while antiinflammatory efficacy, analgesic effect, acute toxicity and in situ absorption were tested through in vivo experiments. The results obtained were as follows: 1) The solubilities of pranoprofen argininate and lysinate salts were increased markedly in pH 6.8 and pH 7.5 phosphate buffer solutions, comparing with that of pranoprofen itself. 2) $pK_a$ values of pranoprofen, pranoprofen argininate and lysinate salts were 6.34, 7.99 and 7.56 in carbon tetrachloride, and 5.86, 6.69 and 7.92 in chloroform, respectively by liquid-liquid partition method. 3) The lipid-water partition coefficients of pranoprofen argininate and lysinate salts were increased more than that of pranoprofen in carbon tetrachloride, chloroform, or benzene-pH 6.8 buffer system, but were nearly identical using pH 1.2 buffer as water phase. 4) Antiinflammatory effects of pranoprofen argininate and lysinate salts were remarkably increased and analgesic effects of the salts were as same as that of pranoprofen. 5) Pranoprofen argininate and lysinate salts were safer than pranoprofen itself in acute toxicity, and the in situ absorption rates of pranoprofen, pranoprofen argininate and lysinate salts were 0.392, 0.960 and $0.762\;hr^{-1}$, respectively according to the rat intestine recirculation experiment.

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The Effect of the Oxygen Scavenging System on the pH of Buffered Sample Solutions: in the Context of Single-molecule Fluorescence Measurements

  • Kim, Sung-Eun;Lee, Il-Buem;Hong, Seok-Cheol
    • Bulletin of the Korean Chemical Society
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    • v.33 no.3
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    • pp.958-962
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    • 2012
  • In single-molecule fluorescence experiment, the oxygen scavenging system is indispensable for avoiding photo-bleaching of fluorescent dyes. Here we report that the gloxy-based oxygen scavenging system commonly used in single molecule fluorescence experiments can disturb the solution pH considerably. To track in situ pH change, we utilized the pH-sensitive conformational transition of i-motif and examined the transition with ensemble and single-molecule FRET measurements. Based on our results, we also suggested several practical remedies for the stability of the solution pH.

Thermal Performance of the Microencapsulated PCM

  • Lee, Hyo-Jin;Lee, Jae-Goo
    • International Journal of Air-Conditioning and Refrigeration
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    • v.10 no.1
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    • pp.31-39
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    • 2002
  • Microencapsulated pcm (MPCM) particles are mixed with distilled water and utilized to evaluate its characteristics and performance as a thermal storage medium transporting heat. For the present study, tetradecane ($C_14$$H_30$, $T_m$=5.5$^{\circ}C$) is capsulated in the core, coated with the melamine for their surface. The size of particles is well-controlled under 10$\mu$m in the process of in-situ polymerization with melamine-formaldehyde resin. For the experiment, the concentractions of slurries are prepared for 20 wt%, 30 wt%, and 40 wt%. The results are compared with those of water and 100% tetradecane oil. The pure water and tetradecane start solidifying within 20 minutes after introducing cooling water into the thermal storage tank whose flow rates are varied by 125 cc/min, 250 cc/min, and 500 cc/min. However, MPCM slurries are required relatively longer period of time for their phase change than pure phase change materials. That is, the entrained MPCM particles restrict their heat transfer in terms of natural convection and conduction to them.