• Asian-Australasian Journal of Animal Sciences
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• 제20권8호
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• pp.1208-1214
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• 2007

Three male Gayal, two years of age and with a mean live weight of $203{\pm}26$ kg, and three adult Yunnan Yellow Cattle, with a mean live weight of $338{\pm}18$ kg were fed a ration of pelleted lucerne hay and used to collect rumen fluid for in vitro measurements of digestibilities and gas production from fermentation of a range of forages. The forages were: bamboo stems, bamboo twigs, bamboo leaves, rice straw, barley straw, annual ryegrass hay, smooth vetch hay and pelleted lucerne hay. There were significant (p<0.05) effects of the source of rumen fluid on in vitro dry matter digestibility (IVDMD) and gas production during fermentation of forage. For the roughage of lowest quality (bamboo stems and rice straw), gas production during fermentation was higher (p<0.05) in the presence of rumen fluid from Gayal than Yunnan Yellow Cattle. Differences for these parameters were found for the better quality roughages with gas production being enhanced in the presence of rumen fluid from Yunnan Yellow Cattle. Moreover, the IVDMD of investigated roughages was significantly higher (p<0.05) in Gayal than Yunnan Yellow Cattle. The results offer an explanation for the positive live weight gains recorded for Gayal foraging in their natural environment where the normal diet consists of poor quality roughages.

• Asian-Australasian Journal of Animal Sciences
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• 제8권5호
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• pp.433-441
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• 1995

A series of experiments was conducted to determine the influence of various pepsin-HCL pretreatment factor, hereby the factors of duration of washing for the retrieved bags, inherent to the mobile nylon bag technique (MNBT), on apparent ileal digestibility of crude protein (AIDCP) and apparent ileal digestibility of dry matter (AIDDM). At last, the AIDCP and apparent ileal digestibility of amino acids (AIDAA) in maize, barley, wheat, rapeseed meal, cottonseed meal and three mixed diets were determined with the MNBT and ileo-rectal anastomis pigs (IRAT). For the MNBT techniques, bag measuring $25{\times}40$ MM and containing 0.75 g feedstuff samples, after pre-digestion in vitro, were introduced into the ileo-rectal anastomis pigs (IRAT) gastrointestinal tract through a duodenal cannula and recovered in the ileal digesta between 6 and 12 h. later. 1. The apparent ileal digestibility of dry matter (AIDDM) and crude protein (AIDCP) of the tested samples, with the exception of fish meal, determined by MNBT were not affected by the different pepsin-HCL pretreatment times in vitro between 2.5 h. and 4 h. 2. There was no significant (p > 0.05) difference of the AIDCP and AIDDM of maize determined by the MNBT among different pepsin concentration (0.03%, 0.07% and 0.1 %) treatment in vitro. 3. The AIDCP determined with the MNBT was affected by the washed and unwashed recovered bags from the ileal digesta. 4. The AIDCP and AID amino acids (AIDAA) of maize, barley, wheat, rapeseed meal, soya-bean meal, cottonseed meal and three mixed diets from the MNBT, with a solution of 0.01N HCL (PH 2) and 0.1% of pepsin concentration, a pepsin-HCL pretreatment time in vitro or 4h. and a washing time of the recovered bag from the ileal digesta compared well with those from the IRAT. The linear regression analysis showed a significant correlation (p < 0.01) of AIDCP and AIDDA between the IRAT and MNBT.

• 한국작물학회지
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• 제48권3호
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• pp.276-280
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• 2003

남부지역에 적응한 사초용 유채의 춘파 품종을 선발하기 위하여 3월 25일에 파종한 그 결과는 다음과 같다. 1. 다수성이며 조단백질 함량과 in vitro dry matter digestibility(IVDMD)가 높고 NDF, ADF, cellulose 및 lignin 함량이 낮아서 영양가가 우수한 춘파 용 사초 유채는 Velox 품종이었다. 2. Velox품종은 초장이 길고 경태가 두꺼웠으며 주경의 잎도 많았고 생체중 과 총건물수량이 가장 많았다. 3. 모든 형질에 대한 유전력은 대부분의 형질에서 높았으나 hemicellulose에서만이 낮게 평가되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권6호
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• pp.797-805
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• 2014

The effect of concentrate mixtures with crude protein (CP) levels 10%, 13%, 16%, and 19% and diets with roughage to concentrate ratios 80:20, 60:40, 40:60, and 20:80 (w/w) were determined on dry matter (DM) and organic matter (OM) digestibility, and fermentation metabolites using an in vitro fermentation technique. In vitro fermented attributes were measured after 4, 24, and 48 h of incubation respectively. The digestibility of DM and OM, and total volatile fatty acid (VFA) increased whereas pH decreased with the increased amount of concentrate in the diet (p<0.001), however CP levels of concentrate did not have any influence on these attributes. Gas production reduced with increased CP levels, while it increased with increasing concentrate levels. Ammonia nitrogen ($NH_3$-N) concentration and microbial CP production increased significantly (p<0.05) by increasing CP levels and with increasing concentrate levels in diet as well, however, no significant difference was found between 16% and 19% CP levels. Therefore, 16% CP in concentrate and increasing proportion of concentrate up to 80% in diet all had improved digestibility of DM and organic matter, and higher microbial protein production, with improved fermentation characteristics.

• Journal of Animal Science and Technology
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• 제63권5호
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• pp.1018-1033
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• 2021

In this study, we aimed to assess the effect of flaking on the nutrient digestibility of corn grain in ruminants. In this regard, in vitro rumen fermentation, in situ rumen degradability, and in vivo metabolic experiments were performed. The automated gas production technique was used for the in vitro fermentation experiments. Six types of corn flakes with various degrees of gelatinization (32%, 41%, 48%, 66%, 86%, and 89%) were ground and incubated in rumen fluid to measure rumen fermentation characteristics and digestion rate. The in situ degradability of ground corn, whole corn, and corn flakes with 62% and 66% gelatinization was measured by incubation in the rumen of two cannulated Holstein cows. In vivo metabolic experiments were performed using 12 crossbred goats (29.8 ± 4.37 kg) using a 3 × 3 Latin square design. The dietary treatments consisted of ground corn and flaked corn with 48% or 62% gelatinization. In vitro experiments showed that as the degree of gelatinization increased, the digestion rate increased linearly, while the discrete lag time decreased linearly (p < 0.05). The effective rumen dry matter degradability, determined by in situ fermentation, was 37%p lower in corn flakes than ground corn, assuming a passage rate of 6%/h (p < 0.01), and there was no difference between the two flakes. In the in vivo experiment, there was no difference in dry matter intake, average daily gain, feed efficiency, and nitrogen utilization among the treatment groups (p > 0.05); however, the crude fat digestibility was lower for corn flakes than for ground corn (p < 0.05). To summarize, the rate of fermentation of corn flakes increased as the degree of gelatinization increased. However, non-ground corn flakes had lower rumen digestibility and did not improve in vivo apparent nutrient digestibility, compared with ground corn. In contrast to the assumption that flaked corn provides more energy to ruminant animals than ground corn, we conclude that the digestibility and energy value of corn flakes are lower than those of ground corn if mastication does not sufficiently reduce the particle size of corn flakes.

• 한국초지조사료학회지
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• 제4권2호
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• pp.89-97
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• 1983

전남지역(全南地域)의 간척지(干拓地), 간사지(干潟地) 및 하천제방(河川堤防) 등에 널리 자생(自生)하고 있는 갈대에 대(對)하여 1982년(年) 5월(月)부터 동년(同年) 10월(月)까지 전남 영암군 군서면 해창리, 함평군 학교면 곡창리, 광양군 진월면 망덕리 지역(地域)의 간척지(干拓地), 하천제방(河川堤防) 및 간석지(干潟地)를 중심(中心)으로 생육시기별(生育時期別), 지역별(地域別) 수량(收量), 영양소(營養素) 함량(含量) 및 in-vitro 건물소화율(乾物消化率)을 조사(調査) 분석(分析)하였는데 그 결과(結果)는 다음과 같다. 1. 갈대의 초장(草長)은 5월(月)과 6월(月)에 급속(急速)히 신장(伸長)하였고, 건물수량(乾物收量)은 9월(月)까지 증가(增加)한 후(後) 감소(減少)하였다. 2. 갈대는 일반적(一般的)으로 생육(生育)이 진행(進行)됨에 따라 조단백질(粗蛋白質) 함량(含量)과 in-vitro 건물소화율(乾物消化率)은 감소(減少)하였고 섬유소(纖維素) 함량(含量)은 증가(增加)하였다. 3. 갈대는 생육지역(生育地域)에 따라 일반성분(一般咸分), $SiO_2$, Ca 및 P 함량(含量)에 상당한 차이(差異)가 나타났다. 4. 갈대의 조단백질(粗蛋白質) 함량(含量)과 섬유소(纖維素) 함량(含量) 사이에는 부(負)의 상관관계(相關關係)가 있었고, 조단백질(粗蛋白質)과 in-vitro 건물소화율(乾物消化率)과는 정(正)의 상관관계(相關關係)가 있었으며, in-vitro 건물소화율(乾物消化率)과 섬유소(纖維素) 함량(含量)과는 부(負)의 상관관계(相關關係)가 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권1호
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• pp.70-74
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• 2007

An in vitro study was carried out to investigate the differences in rumen microbes and fiber degradation capacity between sheep and goats. Three local male sheep and three Inner Mongolia male cashmere goats (aged 1.5 to 2 years; weight 25.0 to 32.0 kg) were each fitted with a permanent rumen cannula used to provide rumen fluid. Cycloheximide was used to eliminate rumen anaerobic fungi. The results showed that the quantities of fungal zoospores in the culture fluid of the control group were significantly greater in the sheep than in the goats; however, bacteria and protozoa counts were significantly higher in goats than in sheep. The digestibility of straw dry matter did not differ significantly between the two species before elimination of fungi, but tended to be higher for sheep (55.4%) than for goats (53.3%). The results also indicated that bacteria counts increased significantly after elimination of anaerobic fungi; however, the digestibility of straw dry matter significantly decreased by 12.1% and 8.6% for sheep and goats respectively. This indicated that the anaerobic fungi of the rumen played an important role in degradation of fiber.

• Asian-Australasian Journal of Animal Sciences
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• 제11권1호
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• pp.13-16
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• 1998

A $2{\times}2{\times}4$ factorial study was conducted to examine the possibility of improving estimates of in vitro digestibility, using untreated (UBS) and ammonia-treated (ABS) barley straw, through homogenization of rumen fluid (RF) and by additions of urea (U) and casein (C) as N sources and Xylose + Glucose (XG) as energy sources into artificial saliva. Digestibiltiy of ABS was significantly greater than that of UBS (p < 0.001). There was a significant decrease in digestibility when additions (U, UC, UCXG) were compared with the control (p < 0.001). A 2-way interaction between RF and straw type was significant (p < 0.05) for dry matter digestibility (DMD). Homogenization of RF increased DMD of ABS (p < 0.05) whereas it decreased DMD of UBS (p > 0.05). The study showed that addition of N alone or in combination with energy sources was not better than control, rather the reverse, digestion was inhibited by a combination of U and casein (UC). It was concluded that sufficient N and branched chain fatty acids were supplied in the inoculum from sheep fed high protein grass cubes to support the growth of cellulolytic microbes during in vitro incubation. Further studies are, however, required to explore the potential of homogenization in improving the in vitro method to estimate digestibility of cereal straws.

• Asian-Australasian Journal of Animal Sciences
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• 제17권2호
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• pp.207-212
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• 2004

Ruminal digestion of dry matter (DM) and neutral detergent fiber (NDF) of processed (ensiled, deepstacked or composted) broiler litter (BL) was determined in situ and in vitro, and compared with rice straw (RS). DM disappearances at 24 and 48 h and digestion of differently processed BL were higher than those of RS. Compared with RS, processed BL was low in NDF disappearance at 72 h incubation, digestion rate ($K_dB$) and digestibility at 0.025 of passage rate; however, deepstacked BL was similar in these NDF characteristics. Processing of BL affected ruminal digestion of nutrients such as DM and NDF adversely. NDF of composted BL, especially, was the most indigestible. This in situ nutritional evaluation indicated that deepstacked BL, the most widely used form of BL, was superior in DM characteristics (fractions, ruminal disappearance and digestibility) and similar in NDF characteristics (ruminal disappearance and digestibility) to RS.

• Asian-Australasian Journal of Animal Sciences
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• 제13권8호
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• pp.1113-1120
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• 2000

Productivity, nutrient contents, in vitro gas production and in sacco degradability of different fractions and whole OPF were determined to assess the feeding value of OPF as a ruminant feed. An in vivo digestibility trial was also carried out using goat. Freshly harvested OPF was randomly collected, partitioned and weighed. An OPF from 21 years older palm weighed 13.4 kg and the annual fresh matter yield of petiole, leaflet and midrib was 46.5, 11.8 and 3.4 ton/ha, respectively. Leaflet contained 439, 926, 698, 501, 168, 196, 748 and 52 (g/kg) of dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose (CE), hemicellulose (HC), total carbohydrate (TC) and non fiber carbohydrate (NFC), respectively. Petiole contained lower (p<0.01) DM, CP and EE contents than leaflet. Organic matter, CE and TC contents were higher (p<0.01) in petiole compared to leaflet. Silica and lignin contents were highest (p<0.01) in midrib followed by leaflet, whole OPF and least in petiole. The Ca, P, Na, K and Mg contents (g/100 g DM) of leaflet were 0.529, 0.182, 0.039, 0.876, and 0.168, respectively. In vitro DM digestibility (g/100 g) at 48 h of leaflet, petiole and midrib was 32.7, 38.7 and 30.2, respectively. The in sacco DM degradation (g/100 g) at 48 h of leaflet was higher than that of whole OPF, petiole and midrib. The in vivo digestibility of DM, OM, CP and ADF of whole OPF was 52, 56, 43 and 26%, respectively. It can be concluded that leaflet is the most nutritious fraction of OPF and midrib is the least. The nutrient content and digestibility of the whole OPF showed that OPF could be an alternative roughage source for ruminant diets.