Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Fungal Elimination on Bacteria and Protozoa Populations and Degradation of Straw Dry Matter in the Rumen of Sheep and Goats

  • Li, D.B. (College of Animal Science and Medicine, Inner Mongolia Agricultural University) ;
  • Hou, X.Z. (College of Animal Science and Medicine, Inner Mongolia Agricultural University)
  • Received : 2005.12.02
  • Accepted : 2006.04.13
  • Published : 2007.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

An in vitro study was carried out to investigate the differences in rumen microbes and fiber degradation capacity between sheep and goats. Three local male sheep and three Inner Mongolia male cashmere goats (aged 1.5 to 2 years; weight 25.0 to 32.0 kg) were each fitted with a permanent rumen cannula used to provide rumen fluid. Cycloheximide was used to eliminate rumen anaerobic fungi. The results showed that the quantities of fungal zoospores in the culture fluid of the control group were significantly greater in the sheep than in the goats; however, bacteria and protozoa counts were significantly higher in goats than in sheep. The digestibility of straw dry matter did not differ significantly between the two species before elimination of fungi, but tended to be higher for sheep (55.4%) than for goats (53.3%). The results also indicated that bacteria counts increased significantly after elimination of anaerobic fungi; however, the digestibility of straw dry matter significantly decreased by 12.1% and 8.6% for sheep and goats respectively. This indicated that the anaerobic fungi of the rumen played an important role in degradation of fiber.

Keywords

References

  1. Alan, G., N. Williams, Keith Joblin and G. Fonty. 1994. Interactions between the rumen chytrid fungi and other microorganisms. In: Anaerobic Fungi, 12nd (Ed. D. O. Mountfort and C. G. Orpin). Marcel Dekker, Inc. 207 Madison Avenue, New York. pp. 191-227.
  2. Akin, D. E. and W. S. Borneman. 1990. Role of rumen fungi in fiber degradation. J. Dairy Sci. 73:3023-3032. https://doi.org/10.3168/jds.S0022-0302(90)78989-8
  3. Akin, D. E. and L. L. Rigsby. 1987. Mixed fungal populations and lignocellulosic tissue degradation in the bovine rumen. Appl. Environ. Microbiol. 9:1987-1995.
  4. Bauchop, T. 1979. Rumen anaerobic fungi of cattle and sheep. Appl. Environ. Microbiol. 38:148-158.
  5. Bauchop, T. 1981. The anaerobic fungi in rumen fiber digestion. Agric. Environ. 6:339-348. https://doi.org/10.1016/0304-1131(81)90021-7
  6. Chen, G. and J. B. Russell. 1988. Fermentation of peptides and amino acids by a monensin-sensitive ruminal peptostreptococcus. Appl. Environ. Microbiol. 54:2742-2749.
  7. Domingue, B. M. F., D. W. Dellow and T. N. Barry. 1991. Voluntary intake and rumen digestion of a low-quality roughage by goats and sheep. J. Agric. Sci. Camb. 117:111-120. https://doi.org/10.1017/S002185960007903X
  8. Fazaeli, H., H. Mahmodzadeh, A. Azizi, Z. A. Jelan, J. B. Liang, Y. Rouzbehan and A. Osman. 2004. Nutritive Value of Wheat Straw Treated with Pleurotus Fungi. Asian-Aust. J. Anim. Sci. 17:1681-1688. https://doi.org/10.5713/ajas.2004.1681
  9. Hadjigeorgiou, I. E., I. J. Gordon and J. A. Milne. 2001. The intake and digestion of a range of temperate forages by sheep and fibre-producing goats. Small Rumin. Res. 39:167-179. https://doi.org/10.1016/S0921-4488(00)00189-9
  10. Hillaire, M. C., J. P. Jouany and G. Fonty. 1990. Wheat straw degradation, in rusitec, in the presence and absence of rumen anaerobic fungi. Proceed. Nutr. Soc. 49:127A.
  11. Ho, Y. W. and N. Abdulan. 1999. The role of rumen fungi in fibre digestion. Asian-Aust. J. Anim. Sci. 12:104-112. https://doi.org/10.5713/ajas.1999.104
  12. Huston, J. E., B. S. Engdahl and K. W. Bales. 1988. Intake and digestibility in sheep and goats fed three forages with different levels of supplemental protein. Small Rumin Res. 1:81-92. https://doi.org/10.1016/0921-4488(88)90047-8
  13. Mao, S. Y. 2001. Isolation and purification of anaerobic fungi from the rumen and faeces of goats and comparison of fermentation activity between isolates in vitro. Master. Thesis, Nan Jing Agricultural University, Nan Jing, China.
  14. Joblin, K. N. 1981. Isolation, enumeration, and maintenance of rumen anaerobic fungi in roll tubes. Appl. Environ. Microbiol. 42:1119-1122.
  15. Joblin, K. N. and G. Naylor. 1989. Fermentation of woods by rumen anaerobic fungi. Fems Microbiol. Letters. 65:119-122. https://doi.org/10.1111/j.1574-6968.1989.tb03608.x
  16. Jouany, J. P. 1989. Effect of diet on populations of rumen protozoa in relation to fiber digestion. In: The role of protozoa and fungi in ruminant digestion (Ed. J. V. Nolan, R. A. Leng and D. I. Demeyer). Armidale, Australia: Penambul Books. pp. 59-74.
  17. Martin, C. and E. Devillard. 1999. Influence of sampling site on concentrations and carbohydrate-degrading enzyme activities of protozoa and bacteria in the rumen. J. Anim. Sci. 77:979-987. https://doi.org/10.2527/1999.774979x
  18. Orpin, C. G. 1977. Studies on the defaunation of the ovine rumen using dioctyl sodium sulphosuccinate. J. Appl. Bacteriol. 43:309-318 https://doi.org/10.1111/j.1365-2672.1977.tb00756.x
  19. Ramanzin, M., L. Bailoni and S. Schiavon. 1997. Effect of forage to concentrate ratio on comparative digestion in sheep, goats and fallow deer. Anim. Sci. 64:163-170. https://doi.org/10.1017/S1357729800015678
  20. Richardson, A. J., C. S. Stewart and G. P. Campbell. 1986. Influence of co-culture with rumen bacteria on the lignocellulolytic activity of phycomycetous fungi from the rumen. XIV Intl. Congress Microbiol. 233:2-24 (Abstr).
  21. Taylor, G. T. and S. J. Pirt. 1977. Nutrition and factors limiting the growth of a methanogenic bacterium (Methanobacterium thermoautotrophicum). Arch Microbiol. 113(1-2):17-22. https://doi.org/10.1007/BF00428574
  22. Van Os M., J. P. Dulphy and R. Baumont. 1995. The effect of protein degradation products in grass silages on feed intake and intake behaviour in sheep. Br. J. Nutr. 73:51-64. https://doi.org/10.1079/BJN19950008
  23. Yanez Ruiz, D. R., A. Moumen, A. I. Martin Garcia and E. Molina Alcaide. 2004. Ruminal fermentation and degradation patterns, protozoa population, and urinary purine derivatives excretion in goats and wethers fed diets based on two-stage olive cake: effect of PEG supply. J. Anim. Sci. 82:2023-2032. https://doi.org/10.2527/2004.8272023x

Cited by

  1. In vitro rumen fermentation characteristics of goat and sheep supplemented with polyunsaturated fatty acids vol.57, pp.8, 2017, https://doi.org/10.1071/AN15684
  2. The Effect of a High-Grain Diet on the Rumen Microbiome of Goats with a Special Focus on Anaerobic Fungi vol.9, pp.1, 2021, https://doi.org/10.3390/microorganisms9010157