• 대한수의학회지
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• 제42권3호
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• pp.351-362
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• 2002

Newcastle disease (ND) is a highly contagious infection of poultry, Two pathology-based techniques, in situ RT-PCR and in situ hybridization (ISH) were applied to formalin-fixed, paraffin-embedded tissues from chickens naturally infected with velogenic ND virus (VNDV). Two pairs of primers and a probe for ISH and in situ RT-PCR, respectively, were selected from highly conserved region of matrix gene of NDV. The ISH experiment was carried out using MicroProbe$^{TM}$ capillary action system within 2 hours. In situ RT-PCR was performed using MicroProbe$^{TM}$ capillary action system and GeneAmp In Situ PCR system. With ISH and in situ RT-PCR, viral nucleic acid was detected in the central nervous system of chickens from infected with neurotropic velogenic Newcastle disease virus (NVNDV), whereas viral nucleic acid was detected in various organs or tissues of chickens from infected with viscerotropic velogenic Newcastle disease virus (VVNDV). In the NVND group, positive signals were characteristically defined in the cytoplasm of neuron, vascular endothelial cells, and perivascular mononuclear macrophages in the central nervous system. One of NVND group, chicken from one farm exhibited positive signals in the bronchial epithelium. The VVND group chickens showed positive reaction in the macrophages, vascular endothelium, and bronchiolar epithelium. Markedly, viral nucleic acid was detected in the macrophages of morphologically normal tissues which were peripheral or located in distant areas from lesions. The central nervous system of chickens infected with VVND virus had positive signals in the vascular endothelial cell, perivascular mononuclear macrophages and some neuron. The number and intensity of the positive cells by in situ RT-PCR were more and stronger, respectively, in comparison with those by ISH. Particularly, positive reaction was detected in macrophages infiltrating in cardiac muscle by in situ RT-PCR, but not obtained by ISH. Therefore, these results demonstrated that ISH is a rapid diagnostic method for detection of NDV and in situ RT-PCR can be used as an efficient method for detection of low viral load infection or subclinical viral infection of NDV.

• 대한의생명과학회지
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• 제2권2호
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• pp.257-265
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• 1996

토끼 바이러스성 출혈증의 원인체를 실험 토끼에 접종하여 증식을 유도하고 간장에서 hematoxylin & eosin 염 색 에서 조직학적 진단과 세포내 viral RNA의 소재를 결정하기 위해 post-unicryl 포매한 block의 절편을 사용하여 단 염색과 전자현미경적 in situ hybridization을 시도하였다. 토끼 출혈증 viral RNA의 보합 결합에 이용하는 probe는 4717에서 4800(84bases)까지 oligonucleotide를 5'말단에 biotin-CE phosphoramidite로 표지하여 사용하였다. 보합결합물의 증명은 신호 표지로서 antibiotin antibody-l0nm gold를 사용하였으며, hybridization이나 증명은 기존 protocol에서 약간의 변법을 사용하였다. 0.02% glutaraldehyde에서 고정하고 unicryl resin 포매한 표본, biotinylated oligonucleotide probe, antibiotin antibody-l0nm gold로 실험한 결과 증강된 신호를 얻을 수 있었다. 특히 전처리를 생략하므로써 실험 과정을 간단하게 하여 신속한 결과를 얻을 수가 있었다. 전자현미경 in situ hybridization을 통하여 토끼 출혈증 바이러스의 주요 표적은 간세포로 감염 세포의 세포질 내 미토콘드리아와 핵 사이에서 immune gold입자가 뚜렷하게 표지 됨으로서 viral RNA를 증명할 수 있었다.

• 한국음향학회지
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• 제11권2호
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• pp.15-27
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• 1992

헤드폰을 사용하여 청각실험을 하는 경우에 합리적인 헤드폰 측정방법이 확립되지 않아 그 측정방법에 따라 다른 결과를 초래하는 문제점이 있기 때문에, 헤드폰의 주파수 특성에 관한 표준 측정방법이 요구된다. 본고에서는 이러한 중요성에 입각하여 종래의 의사귀를 이용한 측정방법과 최근 새롭게 제안되고 있는 HATS를 이용한 측정방법을 사용하여, 사람에 있어서 자연적인 청취상태에 입각한 헤드폰 측정방법에 대해 고차하고, 종래의 측정방법과 비교 검토하였다. 그 결과 사람의 귀의 음향 임피던스와 머리의 회절요과를 정확히 모사하지 못한 의사귀 측정방법에 비해, 이 문제점을 해결한 HATS를 이용한 측정방법에서 사람에 있어 보다 자연적인 청취상태에 입각한 주파수 특성을 측정할 수 있었다.

• 대한바이러스학회지
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• 제30권2호
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• pp.125-130
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• 2000

Infection with human cytomegalovirus (HCMV) is of considerable clinical relevance after placental transmission and in immunosuppressed patients such as transplant recipients or patients with AIDS. The rapid detection method of HCMV has been required to overcome the time-consuming methods such as classical plaque assay or other immunological methods. This study was performed to establish the in situ ELISA, in which human lung fibroblasts infected with HCMV were fixed and used directly as antigen in 96 well culture plate. Expressed HCMV antigens were detected with HCMV-specific monoclonal antibodies. This method could detect HCMV dose-dependently upto $3{\times}10^2\;pfu/ml$. Antiviral activity of ganciclovir could be assayed within the known range of effective dose. This result showed that HCMV could be quantitated by in situ ELISA. The chemical, which was selected on the basis of component analysis in natural product, was tested to have the anti-HCMV activity by in situ ELISA, and three among five samples were found to have anti-HCMV activity with the dose-dependent manner. Conclusively in situ ELISA could be useful method for quantitation of HCMV and screening antiviral activity of samples to HCMV.

• 한국터널지하공간학회 논문집
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• 제9권3호
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• pp.275-286
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• 2007

지하암반에서 지하구조물을 구축하는 경우, 지하구조물의 안정성 확보를 위한 굴착, 지보 및 대책공법은 현지 암반응력의 크기 및 방향에 큰 영향을 받게 된다. 따라서 터널과 같은 지하구조물의 설계와 안정성 해석에 있어서 현지 암반응력의 분포특성을 고려하는 것은 대단히 중요한 문제라고 할 수 있다. 본 연구에서는 암반응력을 고려한 터널설계 사례를 통하여 합리적이고, 안정적인 지하구조물 설계 방안에 대하여 고찰하고자 하였다 이를 위하여 Q-System에 의한 암반분류시 입력변수인 응력저감계수(SRF) 평가과정 및 표준지보패턴 설계 사례에 대하여 검토하였다. 또한 터널안정성 해석시 측압계수 크기 및 주응력 방향을 변수로 한 수치해석을 수행하여 터널 안정성 해석시 개선사항에 대하여 제안하였다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2007년도 추계학술대회논문집
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• pp.987-990
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• 2007

The accuracy of sound strength (G) measurement method in ISO 3382 has been questioned. One of the main reason is the difficulty in measuring a reference sound level in an anechoic chamber with the same set-up which was applied for the actual hall measurements. In the present paper, an in-situ measurement method for G was proposed by investigating the present G measurement method shown in ISO 3382. In addition, the sound radiation characteristics of typical omnidirectional loudspeakers were investigated and Phi (O) of auto correlation function (ACF) parameters was also calculated from an actual music excerpt to characterize the sound energy distribution in concert halls.

• Geomechanics and Engineering
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• 제8권6호
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• pp.783-799
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• 2015

In this study, effect of horizontal in situ stress on failure mechanism around underground openings excavated in isotropic, elastic rock zones is investigated. For estimating the plastic zone occurrence, an induced stress influence area approach (Bray Equations) was modified to define critical stress ratio according to the Mohr-Coulomb failure criterion. Results obtained from modified calculations were compared with results of some other analytical solutions for plastic zone thickness estimation and the numerical modelling (finite difference method software, FLAC2D) study. Plastic zone and its geometry around tunnels were analyzed for different in situ stress conditions. The modified equations gave similar results with those obtained from the other approaches. However, safer results were calculated using the modified equations for high in situ stress conditions and excessive ratio of horizontal to vertical in situ stresses. As the outcome of this study, the modified equations are suggested to use for estimating the plastic zone occurrence and its thickness around the tunnels with circular cross-section.

• 한국농공학회논문집
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• 제50권5호
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• pp.73-81
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• 2008

This study evaluated the applicability of in-situ concrete pile as a stabilization materials of embankment slopes including agricultural reservoir and rural road etc. The experimental embankment slopes was constructed to investigate the strength properties of in-situ concrete pile with embankment materials and boring methods. The test variable were applied the boring method(driving and augering) and water-cement ratio. In order to analyze the physical and mechanical properties of embankment materials, permeability and water contents test were was performed. Also, the freshly and harden of in-situ concrete properties were measured by the slump and compressive strength tests. The results showed the water content and permeability of embankment materials and boring methods affected on compressive strength of in-situ concrete pile.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2006년도 추계학술대회 논문집 Vol.19
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• pp.278-279
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• 2006

In this study, we investigated the electro-optical (EO) characteristic of fringe-field switching (FFS) mode cell by the two kinds of ultraviolet (UV) alignment method on the organic thin film (polyimide Pl). The suitable organic layers for FFS cell and the aligning capabilities of nematic liquid crystal (NLC) using the in-situ photo-alignment method were studied. An unstable V-T curve of UV-aligned FFS-LCD with conventional photo-alignment method can be achieved. However, a stable V-T curve of UV-aligned FFS-LCD with in-situ photo-alignment method (1h), and V-T curve of UV-aligned FFS-LCD with in-situ photo-alignment method was much stable comparing with that of other UV-aligned FFS-LCD's, As a result, more stable EO performance of UV-aligned FFS-LCD with in-Situ photo-alignment method is obtained than that of the other UV-aligned FFS-LCD's.

• 한국전기전자재료학회논문지
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• 제35권5호
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• pp.431-438
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• 2022

기능성 소재연구에서 in situ 분석 기법은 외부 자극 (전기장, 자기장, 빛, 등) 또는 주변 환경 (온도, 습도, 압력, 등)과 같이 주어진 자극에 의해 소재의 물리적 특성이 어떻게 활성화/진화되는지 분석하는데 있어서 매우 중요하다. 특히, 전기장 인가에 따른 in situ X-선 회절(XRD) 실험은 다양한 강유전체, 압전체, 전왜 재료의 외부 전기장 인가에 따른 전기-기계적 반응의 기본 원리를 이해하기 위해 광범위하게 활용되었다. 본 튜토리얼 논문에서는 일반 실험실 규모의 XRD 장비를 이용하여 전기장 인가에 따른 in situ XRD 분석의 기본 원리/핵심 개념을 간략하게 소개한다. In situ XRD 측정법은 외부 전기장을 인가하여 구동되는 다양한 전기-기계 재료의 구조적 변형을 체계적으로 식별/모니터링하는 데 매우 유용할 것으로 기대한다.