• Macromolecular Research
• /
• v.12 no.1
• /
• pp.107-111
• /
• 2004

We have studied the polymerization kinetics of acrylamide in aqueous solution with potassium persulfate as an initiator by using quantitative real-time in situ IR spectroscopy and monitoring the profiles of peaks in the range 1900-850 cm$\^$-1/. The conversion of acrylamide was calculated from the disappearance of the peak at 988 cm$\^$-1/, which is the out-of-plane bending mode of the=C-H unit, normalized to the C=O stretching peak at 1675 cm$\^$-1/, as an internal standard. For reaction temperatures in the range 40-65$^{\circ}C$ and initiator and monomer concentrations of 0.9-2.6 mmol/L and 0.5-1.1 mol/L, respectively, we deduced that the rate of monomer consumption follows the relation R$\_$p/=k［K$_2$S$_2$O$\_$8/］$\^$0.5/ [Μ］$\^$1.35${\pm}$0.10/. In addition, we obtained activation parameters from an evaluation of the kinetic data.

• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.2
• /
• pp.230-236
• /
• 2018

Objective: Two ex vivo experiments were conducted to verify the effect of barley grain (Nusrat cultivar) treated with alkaline compounds (AC) including alum, ammonium, and sodium hydroxide or cation-exchanged organic extracts (OE) prepared from alfalfa hay, sugar beet pulp and Ulva Fasciata, on extent and digestion of starch. Methods: In the first study, the in vitro first order disappearance kinetic parameters of dry matter (DM), crude protein (CP) and starch were estimated using a non-linear model ($D_{(t)}=D_{(i)}{\cdot}e^{(-k_d{\cdot}time)}+I$, where: $D_{(t)}$ = potentially digestible residues at any time, $D_{(i)}$ = potentially digestible fraction at any time, $k_d$ = fractional rate constant of digestion (/h), I = indigestible fraction at any time). In the second experiment, the ruminal and post-ruminal disappearance of DM, CP, and starch were determined using in situ mobile nylon bag. Results: Barley grains treated with alum and alfalfa extract had a higher constant rate of starch digestion (0.11 and 0.09/h) than others. Barley grain treated with OE had a higher constant rate of CP digestion and that of treated with AC had a higher constant rate of starch digestion (0.08 and 0.11/h) compared with those of the other treatments. The indigestible fraction of starch treated with alum and sugar beet pulp extract was higher than that of the control group (0.24 and 0.25 vs 0.21). Barley grain treated with AC and OE had significant CP disappearance in the rumen, post-rumen and total tract, and also starch disappearance for post-rumen and total tract compared with the untreated (p<0.001). Conclusion: This study demonstrated that AC and OE might have positive effects on the starch degradation of the barley grain. In addition, treating barley grain with alum and sugar beet pulp extract could change the site and extend digestion of protein and starch.

• Asian-Australasian Journal of Animal Sciences
• /
• v.6 no.4
• /
• pp.561-567
• /
• 1993

Twenty Korean native bulls averaging 181 kg body weight were fed a fixed amount (1.5% of live weight) of concentrate and free choice roughage cubes which had four ratios of rice straw and orchard grass hay; 100:0, 85:15, 70:30 and 55:45 on a weight basis. Five bulls were assigned to each treatment and fed for 98 days in a confinement house. Nutrient digestibility and available energy content of mixed rations (40% concentrate and 60% roughage cubes) was determined in a digestion trial with twelve wethers. In vitro dry matter digestibility (IVDMD) and in situ dry matter disappearance of the roughage cubes were also determined. The ratio of grass hay to rice straw did not influence dry matter intake. Significant improvements in body weight gains and feed/gain ratios were obtained as grass hay levels increased. Average daily gain and feed/gain for each treatment was 0.83, 0.88, 0.98 and 0.99 kg; 7.63m 7.59, 6.83 and 6.41, respectively. Digestibility of the nutrients was improved with increasing levels of grass hay in the cubes. The IVDMD of roughage samples having a ritio of 100:0, 85:15, 70:30 and 55:45 between rice straw and orchard grass hay were 31.0, 37.1, 41.8 and 43.4%, respectively. Grass hay improved the IVDMD of rice straw diets in a linear manner up to 30%. In situ dry matter disappearance rate was also increased as the level of orchard grass hay increased.

• Journal of Animal Science and Technology
• /
• v.64 no.6
• /
• pp.1077-1091
• /
• 2022

This study was conducted to determine the rumen fermentation dynamics of lupin flakes and elucidate the effects of lupin flake supplementation on the growth performance, blood metabolites, and carcass characteristics of Hanwoo steers. In vitro and in situ trials of lupin grains and lupin flakes were conducted using three Hanwoo cows with rumen fistulas. The feeding trial included 40 early-fattening Hanwoo steers randomly divided into four groups: control, T1, T2, and T3. Their formula feed contained 0%, 3%, 6%, and 9% lupin flakes, respectively. In vitro rumen pH and ammonia concentrations were lower in the lupin flake group than in the lupin grain group after 6 and 24 h of incubation, respectively (p < 0.05). Concentrations of propionate, butyrate, and total volatile fatty acids were higher in the lupin flake group than in the lupin grain group after 12 h of incubation (p < 0.05), as was the crude protein disappearance rate at 9 and 12 h of rumen fermentation (p < 0.05). Supplementation with lupin flakes did not affect the average daily gain. Compared to that in the control group, dry matter intake was lower in the lupin flake-supplemented groups (p < 0.05); the feed conversion ratio was lower in T2 and T3 (p < 0.05); and plasma total protein concentration in 29-month-old steers was lower in T1 and T3 (p < 0.05). Plasma triglyceride concentration was lower in the lupin flake-supplemented groups than in the control group (p < 0.05). The incidence rate of yield grade A was higher in T1 and T2 than in the control group; the incidence rate of meat quality 1⁺ grade or higher was highest in T2. The carcass auction price was higher in T2 than in the other groups. Overall, compared to whole lupin grains, lupin flakes seem to more substantially affect rumen ammonia concentrations and crude protein disappearance rate. Additionally, we suggest that supplementation with 6% lupin flake formula feed exerts positive effects on the feed conversion ratio, yield grade, and quality grade of Hanwoo steers.

• Asian-Australasian Journal of Animal Sciences
• /
• v.9 no.5
• /
• pp.533-538
• /
• 1996

The rate and extent of digestion of dietary carbohydrates has a tremendous impact on ruminal fermentation and the productivity of the animals. The objective of the study was to determine the dry matter (DM) and neutral detergent fiber (NDF) degradabilities and rate and extent of feed byproducts (cotton seed cake, wheat bran), legumes [berseem (Egyptian clover), lucern (Medicago sativa), cowpeas (Vigna sinensis)], grasses [maize (Zea mays), millet (Panicum miliaceum), sorghum (Sorghum vulgare)] and wheat straw in ruminally fistulated male buffalo calves. By using nylon bags, 10 grams sample was exposed to the ruminal fermentation for 0, 1, 2, 4, 6, 10, 16, 24, 36, 48 and 96 hours. Dry matter and NDF degradability was measured at 48 hours. Extent of DM and NDF disappearance was determined at each time point. Rates of disappearance of DM and NDF were determined by regressing the natural logarithm of the percentage of original DM and NDF remaining in the bags between 1 and 96 hours. The dry matter digestibility (DMD) of the feed byproducts (FBP) and legume forages when incubated in the rumen of male buffalo calves were greater (p < 0.05) than grasses. Extent of digestion followed similar pattern as DMD. Rate of DMD was higher in FBP than in legumes and was the lowest in the wheat straw. The NDF degradability (NDFD) of FBP, legumes and grasses did not differ, however, wheat straw had the lowest NDFD from all the feeds tested. The lowest NDFD of wheat straw may have been due to the depressing effect of lignin on fiber digestion. The FBP and legumes had higher (p < 0.05) rates and lower extents of NDF digestion than grasses.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.6
• /
• pp.793-799
• /
• 2002

Four feeds, three concentrates (rice bran, soybean meal and flaked corn) and one forage (corn silage) were incubated in four ruminally fistulated Holstein steers over three one week periods in a 3${\times}$4 incomplete latin square design where steers served as blocks and feeds as treatment. The objectives of the study were to investigate in situ DM, N and P degradability characteristics of feeds in order to assess availability of these nutrients by ruminants. In each period, all feeds were incubated in quadruplets (corn silage in triplicates) in the rumen of each steer in a reverse order for 3, 6, 9, 12, 18, 24 and 48 h. The DM 'a' fraction was higher and lower (p<0.001) in corn silage and rice bran respectively. Although corn silage contained the lowest (p<0.01) DM 'b' fraction, flaked corn contained the highest. Rate of DM degradation of flaked corn and corn silage were half (p<0.05) of the rate of DM degradation of either rice bran or soybean meal. Potential or effective DM degradability (p<0.05 to 0.001) at various passage rates were the lowest for rice bran and the highest for soybean meal. Corn silage N 'a' and 'b' was the highest and lowest, respectively (p<0.01). N 'c' of corn silage and rice bran was higher (p<0.001) than other feeds. Potential N degradability was the lowest in flaked corn (p<0.05). P 'a' was high (p<0.01) for corn silage and rice bran. P 'b' fraction was very high (p<0.001) in soybean meal but was absent in corn silage. Availability of DM (p<0.01 or 0.001), N (p<0.001) and P (p<0.05) differed between feeds at various passage rates except P availability at k=0.02 per h (p>0.05). The results demonstrate that the availability of DM, N and P by ruminants depends on feed as well as categories of animal.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.6
• /
• pp.800-805
• /
• 2012

Rice straw is an important roughage resource for ruminants in many rice-producing countries. In this study, a rice brittle mutant (BM, mutation in OsCesA4, encoding cellulose synthase) and its wild type (WT) were employed to investigate the effects of a cellulose synthase gene mutation on rice straw morphological fractions, chemical composition, stem histological structure and in situ digestibility. The morphological fractions investigation showed that BM had a higher leaf sheath proportion (43.70% vs 38.21%, p<0.01) and a lower leaf blade proportion (25.21% vs 32.14%, p<0.01) than WT. Chemical composition analysis showed that BM rice straw was significantly (p<0.01) higher in CP (crude protein), hemicellulose and acid insoluble ash (AIA) contents, but lower in dry matter (DM), acid detergent fiber (ADFom) and cellulose contents when compared to WT. No significant difference (p>0.05) was detected in neutral detergent fiber (NDFom) and ADL contents for both strains. Histological structure observation indicated that BM stems had fewer sclerenchyma cells and a thinner sclerenchyma cell wall than WT. The results of in situ digestion showed that BM had higher DM, NDFom, cellulose and hemicellulose disappearance at 24 or 48 h of incubation (p<0.05). The effective digestibility of BM rice straw DM and NDFom was greater than that of WT (31.4% vs 26.7% for DM, 29.1% vs 24.3% for NDFom, p<0.05), but the rate of digestion of the slowly digested fraction of BM rice straw DM and NDF was decreased. These results indicated that the mutation in the cellulose synthase gene could improve the nutritive value of rice straw for ruminants.

• Animal Bioscience
• /
• v.34 no.1
• /
• pp.85-92
• /
• 2021

Objective: The objectives of this study were to determine the in situ degradation parameters and appropriate feeding level of pineapple cannery by-products (PCB) based on the growth performance and blood parameters of growing Hanwoo (Bos taurus coreanae) steers fed various levels of PCB. Methods: Two ruminally cannulated Holstein cows were used for in situ disappearance rate measurements. Nylon bags (5×10 cm, 45 ㎛ pore size) filled with 5 g of PCB in triplicate were inserted into the ventral sac of the two cannulated Holsteins cows and incubated for 0, 2, 4, 8, 16, 24, and 48 h. A total of 16 castrated growing Hanwoo steers (12.5±0.5 months old, 302.9±25.7 kg of initial body weight [BW]) were used for the experiment. Animals were stratified by initial BW and then randomly assigned to one of four experimental diets (0%, 1.5%, 3.0%, or 6.0% of PCB, on the dry matter [DM] basis) fed for 91-d, including 30-d of adaptation. Results: Soluble fraction a of DM and crude protein (CP) was 61.9% and 86.0%, fraction b of DM and CP was 32.7% and 11.2%, and indigestible fraction c of DM and CP was 5.4% and 2.8%. The 6.0% PCB feeding group showed lower productivity compared to animals in the other treatments. Increasing the dietary level of PCB did not alter DM intake, but it was numerically lowest in the 6.0% feeding group. The gain to feed ratio was linearly decreased by increasing of PCB. The quadratic broken-line test estimated that 2.5% (DM basis) was the maximum feeding level of PCB in growing Hanwoo steers (y = 0.103 - 0.001×[1.245-x]2, R2 = 0.18). Conclusion: Diets containing up to 2.5% PCB can be fed to growing Hanwoo steers without adverse effects on growth performance.

• Journal of Life Science
• /
• v.18 no.11
• /
• pp.1561-1569
• /
• 2008

This study was conducted to investigate the effects of steam flaking of corn grains on in situ dry matter degradability in the rumen and contents of mycotoxins (aflatoxin, ochratoxin) during storage. Yellow dent corns imported from USA and India were flaked, and stored for 8 weeks under the standard temperature and pressure (STP; $25^{\circ}C$/ 1 atm.). Experimental treatments were composed of four corn grains (untreated-USA corn, USCW; steam flaked-USA corn, USCF; untreated-India corn, IDCW; steam flaked-India corn, IDCF) with 4 replications according to 9 storage periods (0, 1, 2, 3, 4, 5, 6, 7, 8 week). Two ruminally cannulated Holstein bulls were used for in situ trial. Pathogen contamination trial was performed by comparing the mycotoxin contents in corns during storage periods. Dry matter disappearance rate in the rumen was about 3.0 to 44.1% higher (P<0.05) for USCW than IDCW, but was not difference between USCF and IDCF. With steam flaking of corn, dry matter degradability in the rumen was significantly (P<0.05) increased in corn from India, but was not affected in corn from USA. Aflatoxin content was very low level in corns from USA and steam flaked corns, but was higher than the tolerance limit of domestic aflatoxin content regulation when IDCW was stored over 6 week under STP. Ochratoxin content was low level in all treatments. From above results, it is reasonable that the corn imported from India might be flaked for enhance the ruminal DM degradability and safe from aflatoxin pathogen.

• Asian-Australasian Journal of Animal Sciences
• /
• v.28 no.11
• /
• pp.1606-1613
• /
• 2015

This study was carried out to investigate the effects of Yerba Mate (YM) supplementation on nutrients' degradation, in vitro dry matter disappearance, gas production and rumen ammonia concentration. Three rumen-fistulated Holstein Friesian cows were used for the in situ incubations and provided rumen liquor for in vitro incubations. The inclusion of YM in a control diet (pasture+pellets) affected some in sacco degradation parameters. YM supplementation decreased the effective degradability and degradation rate of pasture crude protein (CP), and it seems to slow down the degradation of pasture neutral detergent fiber. A significant increase of degradation of pasture acid detergent fiber (ADF) was detected after YM inclusion in the control diet. YM supplementation reduced in vitro gas production of pasture and ammonia concentration of pellets. The addition of YM in ruminant diet could decrease ammonia production and increase protein availability for productive purposes. The moderate presence of tannins in YM could have affected the degradation kinetics of pasture CP and ADF and the ammonia production of pellets.